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INDONESIA
Biopropal Industri
Published by Kementerian Perindustrian Republik Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering
Arjuna Subject : -
Articles 208 Documents
 11   12   13   14   15 
PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE OLEH EKSTRAK KASAR ANTOSIANIN UBI JALAR UNGU SEGAR DAN PRODUK OLAHANNYA (Inhibition Activity of α-Glucosidase by Anthocyanin Crude Extract from Purple Sweet Potato and Its Products) Siti Nurdjanah; Neti Yuliana; Danita Aprisia; Azhari Rangga
Biopropal Industri Vol 10, No 2 (2019)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2052.504 KB) | DOI: 10.36974/jbi.v10i2.4765

Abstract

 Purple sweet potato is one of the anthocyanin sources that potential to normalize blood sugar levels in diabetics because it can inhibit α-glucosidase. Since it easily damaged, processing into flour or chips is an alternative to extend the shelf life. This study aimed to determine the content of total phenol, total anthocyanin and inhibition of the α-glucosidase enzyme by crude anthocyanin extract from fresh purple sweet potato, conventional and modified flour and chips. Testing of total phenol, anthocyanin and inhibition of α-glucosidase enzyme activity were carried out using the spectrophotometric method. The results showed that total phenol, anthocyanin and inhibition of α-glucosidase enzyme activity from crude anthocyanin extracts using acid solutions in fresh purple sweet potato were respectively 291.7 mg GAEs/100g, 122.1 mg/100 g; 41.73%; sweet potato flour at 212.1 mg GAEs/100 g, 171.3 mg/100 g; 37.61%; purple sweet potato flour rich in resistant starch  of 182.5 mg GAEs/100 g, 141.1 mg/100 g; 65.59%; purple sweet potato flour partially gelatinized of 193.5 mg GAEs/100 g, 139.4 mg/100 g; 39.91%; and purple sweet potato chips of 299.5 mg GAEs/100 g, 203.6 mg/100 g, 44.73%. The ability of anthocyanin to inhibit the activity of the α-glucosidase enzyme could be increased through the retrogradation of starch and frying into chips.Keywords: α-glucosidase, anthocyanin, diabetic, purple sweet potatoABSTRAKUbi jalar ungu merupakan salah satu sumber antosianin yang berpotensi menormalkan kadar gula darah penderita diabetes karena dapat menghambat enzim α-glukosidase. Ubi jalar ungu segar mudah mengalami kerusakan, sehingga pengolahan dalam bentuk tepung atau keripik merupakan suatu alternatif untuk memperpanjang masa simpan. Penelitan ini bertujuan untuk mengetahui kandungan total fenol, total antosianin serta penghambatan enzim α-glukosidase oleh ekstrak kasar antosianin dari ubi jalar ungu segar, tepung ubi jalar ungu konvensional dan yang termodifikasi secara fisik serta keripik ubi jalar ungu. Pengujian total fenol, antosianin dan penghambatan aktivitas enzim α-glukosidase dilakukan dengan menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa total fenol, antosianin dan  penghambatan aktivitas enzim α-glukosidase dari ekstrak kasar antosianin menggunakan larutan asam pada ubi jalar ungu segar  berturut-turut sebesar 291,7 mg GAEs/100 g, 122,1 mg/100 g; 41,73%; tepung ubi jalar sebesar 212,1 mg GAEs/100 g, 171,3 mg/100 g; 37,61%; tepung ubi jalar ungu kaya pati resisten (TP) sebesar 182,5 mg GAEs/100 g, 141,1 mg/100 g; 65,59%; tepung ubi jalar ungu tergelatinisasi parsial (TG) sebesar 193,5 mg GAEs/100 g, 139,4 mg/100 g; 39,91%; dan keripik  ubi jalar ungu (KU) sebesar 299,5 mg GAEs/100 g, 203,6 mg/100 g; 44,73%. Kemampuan antosianin dalam menghambat aktivitas enzim α-glukosidase dapat ditingkatkan melalui proses retrogradasi pati dalam ubi dan penggorengan ubi jalar ungu menjadi keripik.Kata kunci: α-glukosidas, antosianin, diabetes mellitus, ubi jalar ungu
PREDIKSI STRUKTUR TIGA DIMENSI PROTEIN ALERGEN PANGAN DENGAN METODE HOMOLOGI MENGGUNAKAN PROGRAM SWISS-MODEL - (Prediction of Three-Dimensional Structure from Food Allergen Protein Through Homology Method Using SWISS-MODEL Program) Hendra Wijaya; Fitri Hasanah
Biopropal Industri Vol 7, No 2 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.704 KB) | DOI: 10.36974/jbi.v7i2.692

Abstract

Information about three-dimensional structure of protein is important to clearly understand its character and function in molecular level. Protein structure determination through laboratory testing needs highly cost of steps and instrument. Three dimensional structure of protein can be scientifically predicted using several in silico methods. The structure of allergen protein was predicted using SWISS-MODEL as homology method in this research. The result showed that structure prediction of allergen protein B7TWE7 (UniProt ID) from Fagus sylfatica (beechnut) have three-dimensional structure with identity value 72% and e-value = 3,4x10-58. This study used the 1e09A (PDB ID) of Prunus avinus (cherry) as a template.Keywords: allergen, homology method, protein structure, swiss-model ABSTRAKInformasi struktur tiga dimensi protein penting untuk memahami sifat dan fungsi protein ditingkat molekular secara detil. Penentuan struktur protein dengan cara pengujian di laboratorium memerlukan tahapan dan instrumentasi dengan biaya tinggi. Struktur tiga dimensi protein dapat diprediksi secara ilmiah dengan menggunakan beberapa metode secara in silico. Metode yang digunakan pada penelitian ini adalah metode homologi menggunakan program SWISS-MODEL. Protein yang akan diprediksi struktur tiga dimensinya adalah protein alergen pangan. Hasil penelitian menunjukkan bahwa prediksi struktur protein alergen B7TWE7 (UniProt ID) dari Fagus sylfatica (beechnut) dengan metode homologi menggunakan program SWISS-MODEL menghasilkan model struktur tiga dimensi protein dengan nilai identity 72% dan e-value = 3,4x10-58. Template protein yang digunakan adalah 1e09A (PDB ID) dari Prunus avinus (cherry).Kata kunci: alergen, metode homologi, struktur protein,  swiss-model
Utilization of Cacao Pods Shells For Charcoal Briquettes Suprapti Suprapti; Sitti Ramlah
Biopropal Industri Vol 4, No 2 (2013)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.177 KB) | DOI: 10.36974/jbi.v4i2.814

Abstract

Utilization of cacao pod shells waste into charcoal briquettes is a way to find an alternative energy that can be produced within household scale. The aim of the research is to produce charcoal briquettes from cacao pod shells and its characterization. Research was designed with two factors, which is charcoal size (30, 50, and 70 mesh) and the percentage of starch used (3, 5, and 7%). Charcoal briquette produced then tested its physical and chemical properties. In general, charcoal briquettes from cacao pod shells with a mesh of 30 and 7% starch gives the best results. This charcoal briquette have a moisture content of 6.52%, ash content 16.73%, density 0.96 g/cm3; levels of volatile matter 20.18%, bonded carbon content 56.58%, and calorific value 4163.11 cal./g. Most of the parameters have not been meet the quality standards for charcoal briquettes of the SNI, Japan, England, nor American.
Optimization Process of Virgin Coconut Oil Production to Meet The Quality of Lulur Cosmetics
Biopropal Industri Vol 1, No 2 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.454 KB) | DOI: 10.36974/jbi.v1i2.722

Abstract

Optimization process of virgin coconut oil (VCO) production to meet the  quality of lulur cosmetics was carried out in the Institute for Industrial Research and Standardization Pontianak. This research aimed to  increase the productivity of pure coconut oil both quantity nor quality that were produced so it can meet the quality characteristics as  raw material of  lulur cosmetic. This research was use coconut and pineapple in a local species as the main raw material and other supporting materials such as VCO and others. There were 2 (two) treatment factor used  namely  A (bromelin extract volume) and B (mixing time). The parameters observed were water content, acid number, saponification  number, and free fatty acid (FFA). Results showed that the treatment without bromelin extract  with 5 minutes mixing time were the best treatment for raw material of lulur cosmetic, with the  water content 0.122%, the free fatty acid 0.062%, the acid number 0.174, and saponification  number 261,21  (the condition for oil as the cosmetic raw material was 0.2% max.).
Preliminary Studies with Filler Organoclay Nanocomposite Making for Packaging Yoyon Suyono
Biopropal Industri Vol 3, No 2 (2012)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.732 KB) | DOI: 10.36974/jbi.v3i2.740

Abstract

Clay from West Kalimantan is still used as a raw material in the ceramic industry and it doesn’t have spesific processes for the manufacture of other products. The objective of this study is to determine the effect of organoclay filler on the physical properties of tensile strength clay nanocomposite food packaging applications. The step for clay nanocomposite were produced by purification, milling, organoclay, clay nanocomposite. Clay nanocomposite were characterized by analysis cation exchange capacities (CEC), X-ray diffraction (XRD), particle size analysis (PSA), scanning electron microscopy (SEM), tensile strength test and elongation at break. The purified with hydrochloric acid can increase the value of CEC and remove of the impurities. High energy milling (HEM) technique are with a time of 2 hours gained 225.3 nm sized nanoparticles as raw material organoclay. The addition of 1% organoclay filler increases the tensile strength of nanocomposite clay, according grade 2 and is suitable for second packaging or  not directly contact to food.
ISOLASI DAN IDENTIFIKASI KAPANG ENDOFIT DARI TANAMAN KUNYIT (Curcuma longa L.) SEBAGAI PENGHASIL ANTIOKSIDAN Tiwit Widowati; Bustanussalam Bustanussalam; Harmastini Sukiman; Partomuan Simanjuntak
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.611 KB) | DOI: 10.36974/jbi.v7i1.686

Abstract

Endophyte fungi are microbe that living inside the plant tissue without harming the host plant. Endophyte fungi can produce secondary metabolite which can be used as antioxidant, anticancer and antimicobes compound. Endophyte fungi can be found in many plants especially herbs such as turmeric (Curcuma longa L). The aims of this study are to isolate and identify endophyte fungi from stem of C. longa L. which is potential as an antioxidant producer. The endophyte fungi isolated from turmeric stem were 12 isolates. Antioxidant activity was assayed using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) showed that isolate K.Cl.Sb.B1 produced the highest inhibition value (78,81%). Based on molecular identification, the isolate K.Cl.Sb.B1 was Colletotrichum sp.Keywords: Curcuma longa L., endophyte fungi, identification antioxidant ABSTRAKKapang endofit merupakan mikroba yang terdapat di dalam jaringan tanaman tanpa membahayakan tanaman inang. Kapang endofit mampu menghasilkan metabolit sekunder yang dapat dimanfaatkan sebagai senyawa antioksidan, antikanker dan antimikroba. Kapang endofit dapat ditemukan pada berbagai jenis tanaman terutama tanaman obat seperti kunyit (Curcuma longa L). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang endofit dari batang tanaman kunyit yang berpotensi sebagai penghasil antioksidan. Kapang endofit yang diisolasi dari batang tanaman kunyit diperoleh 12 isolat. Uji antioksidan menggunakan 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) menunjukkan bahwa isolat K.Cl.Sb.B1 menghasilkan nilai inhibisi tertinggi (78,81%). Berdasarkan identifikasi molekuler, isolat K.Cl.Sb.B1 merupakan Colletotrichum sp.Kata kunci: Curcuma longa L., identifikasi antioksidan, kapang endofit
and Antioxidant Activity of Endophytic Fungi from Mahogoni Plant (Swietenia macrophylla King Edward J Dompeipen; Partomuan Simanjuntak
Biopropal Industri Vol 6, No 1 (2015)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.689 KB) | DOI: 10.36974/jbi.v6i1.829

Abstract

Diabetes mellitus is a degenerative disease characterized by hyperglycemia due to insulin insulin deficiency either absoluteor relative. This study was conducted to isolate endophytic fungi from plant twigs mahogany (Swietenia macrophylla King) which active as antidiabetic and antioxidant. Antidiabetic activity conducted by using the α-glucosidase inhibitory and antioxidant activity using free radical reduction method with reagent 2.2-diphenyl-1-picrylhydrazyl (DPPH). Isolation of microbes conducted in the media Corn Meal Malt Agar (CMMA) and Potato Dextrose Agar (PDA) which 7 isolates of fungus in total. Inhibitory activity against α-glucosidase to extract the filtrate and biomass of the isolates A.Sm.2F (72.59 and 92.22%), A.Sm.3F (81.87 and 79.37%), B.Sm.1F (63.40 and 98.84%), B.Sm.2F (65.60 and 62.72%), B.Sm.3F (93.91 and 51.48%), B.Sm.4F (87.48 and 74.64%) thus has potential as an antidiabetic activity. B.Sm.1F was the only isolates active as antioxidants with IC50 of 84.41.
Debittering of Pontianak Orange Juice using Naringinase Enzyme Farid Salahudin
Biopropal Industri Vol 1, No 1 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5012.892 KB) | DOI: 10.36974/jbi.v1i1.742

Abstract

The goal of this research is debittering of orange juices with enzimatic process. Naringinase enzim is produced with Penicillium decumbens. The research metode is complete random design with two variabel. The first variabel is concentration of enzim : 0,0; 200; 400 ppm and the second variabel is time of incubation : 1, 2 and 3 hours. The product of this research is tested ascorbic acid, Total Plate Count and organoleptic test. The result showed concentration of naringinase enzim 400 ppm and incubation 3 hours can eliminated the after taste of orangge juice without reduce the ascorbic acid and colour of orange juice.
Karakteristik Membran Asimetris Polietersufone (PES) dengan Pelarut Dimetil Formamide dan N-Metil-2-Pyrolidone - (Characteristic of Poliethersulfone (PES) Asymmetric Membrane with Dimethyl Formamide and N-Methyl Pyrolidone as Solvent) Sri Mulyati; Fachrul Razi; Zuhra Zuhra
Biopropal Industri Vol 8, No 1 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (797.947 KB) | DOI: 10.36974/jbi.v8i1.1617

Abstract

Membrane that is generally used for separation process could be made using phase inversion technique. This research aims to create polyethersulfone (PES) asymmetric membranes via phase inversion technique using solvent and Trans Membrane Pressure (TMP) as variable. SEM analysis indicated that membranes had asymmetric structure that consits of two layers which denser skin layer on the top surface and the porous support on the bottom. PES/DMF membrane showed larger pore structure than PES/NMP. The permeability coefficients of both membranes were in the ultrafiltration range. The coefficient permeability (Lp) of PES/DMF membrane was 35.769 L/m2.hour, much greater compared to PES/NMP membrane which was 15.364 L/m2.hour.bar. Molecular Weight Cut-Off (MWCO) of PES/DMF membrane was 177 Kda, meanwhile PES/NMP was 186 Kda. Performances of the membranes were evaluated  using dextrane as feed solution. PES/DMF membrane resulted in an higher flux and lower rejection than PES/NMP.Keywords: asymmetric membrane, membrane performance, Molecular Weight Cut-Off (MWCO), morphology, permeability ABSTRAKMembran yang umumnya digunakan untuk proses pemisahan dapat dibuat menggunakan teknik inversi fasa. Penelitian ini bertujuan untuk mengetahui karakteristik membran asimetris polietersulfone (PES) yang dibuat menggunakan teknik inversi fasa dengan variabel jenis pelarut dan Trans Membrane Pressure (TMP). Hasil analisis Scanning Electron Microscopy (SEM) terhadap morfologi membran membuktikan bahwa membran yang dihasilkan merupakan membran asimetris yang terdiri dari dua lapisan yaitu bagian atas merupakan lapisan tipis dan lapisan bawah adalah lapisan berpori. Membran PES/DMF memiliki struktur pori yang lebih besar dibandingkan membran PES/NMP. Koefisien permeabilitas kedua membran yang dihasilkan berada dalam jangkauan ultrafiltrasi. Koefisien permeabilitas (Lp) membran PES/DMF sebesar 35,769 L/m2.jam, nilai ini jauh lebih besar dibandingkan PES/NMP yaitu 15,364 L/m2.jam.bar. Molecular Weight Cut-Off (MWCO) dari membran PES/DMF yaitu 177 Kda sedangkan membran PES/NMP sebesar 186 Kda. Kinerja membran PES/DMF terhadap pemisahan larutan dekstran memberikan nilai fluks yang lebih tinggi daripada membran PES/NMP sedangkan rejeksi yang dihasilkan lebih rendah. Fluks tertinggi diperoleh pada TMP 2 bar sebesar 11,4 L/m2.jam untuk membran PES/DMF dan 10,2 L/m2.jam untuk membran PES/NMP.Kata kunci:          kinerja membran, membran asimetris, Molecular Weight Cut-Off (MWCO), morfologi, permeabilitas
Fermentative Extraction of Coconut Oil to Maintain A Quality of Medium Chain Fatty Acid Farid Salahudin; Nana Supriyatna
Biopropal Industri Vol 5, No 1 (2014)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.781 KB) | DOI: 10.36974/jbi.v5i1.820

Abstract

Coconut oil is healthy vegetable oil because it contains Medium Chain Fatty Acid (MCFA). The used of bleaching agent and excessive heating in coconut oil process will produce low quality oil (rancid). Therefore, it is necessary to processing that does not use chemicals and excessive heating such as fermentation using microbe and enzyme. The aim of this study was to find out the effect of bromelin enzyme concentration and Saccharomyces cereviceae fermentation to MCFA content in coconut oil. This research was done by adding the enzyme bromelain at 0, 50, 100, 200 and 400 ppm and S. cereviceae inoculated in coconut milk. The resulting oil is then tested the water content, acid number and fatty acid content. The result showed that fermentation with 200 ppm bromeline enzyme and S. cereviceae inoculation can produce the best quality coconut oil containing MCFA that meet the APCC standard.

Page 13 of 21 | Total Record : 208

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