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Proceeding of International Congress
Published by Universitas Slamet Riyadi
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Articles 30 Documents
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ANTIBACTERIAL ASSESSMENT OF ALOE VERA (Aloe vera chinensis Linn) CORTEX EXTRACT AND ITS APPLICATION AS INHIBITOR FOR KING PRAWNS (Macrobrachium rosenbergii Linn) SPOILAGE DURING STORAGE AT 5oC Muhammad Nur Cahyanto, Sutardi Andri Nofreeana
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

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Abstract

Aloe vera (Aloe vera chinensis Linn) cortex contains antibacterial compounds such as aloe emodin andaloin, that have potency as natural preservative for foods. The objectives of the research were to determinethe minimum concentration of aloe vera cortex extract to inhibit bacterial growth, and the effects of soakingin the extract and storage at 5oC on the quality parameters of king prawns. The concentrations of extractwere varied i.e. 125, 250, 500 and 1000 μg/ml, respectively, and minimum concentration of extract forinhibition of Pseudomonas fluorescens was determined by Minimum Inhibitory Concentration (MIC)method. Antibacterial compounds in the extract were analyzed by HPLC and TLC methods. TPC, TVB‐N,TMA, pH and organoleptic characteristics of soaked king prawns were evaluated. The results showed 500μg/ml aloe vera cortex extract was an optimum concentration, and soaking for 90 minutes was able toinhibit and to reduce one log cycle of bacteria population. Aloe vera cortex extracts contained 18.21 ppmaloe emodin and 15.5 mg/g aloin (1.55%). After 6 days storage at 5oC, therefore the king prawns was performedby TPC 3.9 x 105, TVB‐N 28,6, TMA 4.60 and pH 7.7. The best quality of king prawns was resultedby 60 minutes soaking with organoleptic score 7.Keywords: Aloe vera; antibacterial; aloe emodin; aloin; king prawns.
FERMENTATION IN SALT SOLUTION TO PRODUCE JACK BEANS (Canavalia ensiformis L) SAUCE Yusman Taufik, Yudi Garnida
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

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Abstract

The purpose of this research was to determine the proportion or optimal formulation so as to produce jackbean sauce best quality using Design Expert D‐Optimal method. This research uses statistical data processingprogram for which the software Design Expert 7.0.0 with Mixture D‐optimal design to design variablethe jack bean (X1), concentration of salt (X2), Rhizopus sp (X3), garlic (X4), coriander (X5), anise(X6), turmeric (X7), bay leaves (X8), leaves of lemongrass (X9), galangal (X10), MSG (X11), brown sugar(X12), and keluak (X13). Analyzed response were cyanide acid content, protein content, water content,total dissolved solids, viscosity, flavor, color, consistency, and aroma. Selected jack bean sauce withoptimal formulation is sauce with the following composition jack bean 12,5%; salt solution 20%; Garlic1,1%; coriander 0,5%; anise 0,05%; turmeric 0,5%; bay leaf 0,6%; lemongrass leaves 0,6%; galangal 1,2%;MSG 0,4%; brown sugar 60,5%;keluak 2%. The formulation has been predicted by the program with levelsof cyanide 13,79 mg/kg; protein content of 3,46%; water content of 28,16%; total dissolved solids 20.58 oBrix; viscosity 21.91 d.Pa.s; the flavor attribute scores 4.52; the color attributes score 4.55; the viscosityattributes scores on 3.96; and the aroma attributes score 3.63.Keywords: Formulation; Jackbeans; Soy Sauce; Design Expert; D‐Optimal.
MONITORING OF Eschericia. coli O157 FROM RAW COW’S MILK IN THE STORAGE TANK IN SLEMAN DISTRICT, YOGYAKARTA Tri Yahya Budiarso, Marcella Indah Kristanti,
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

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Abstract

Escherichia coli O157 is a member of Enterobacteriaceae which has somatic antigen O157. E. coli O157 isassociated with life threatening diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome(HUS) and thrombotic thrombocytopenic purpura (TTP). Raw milk is considered a high risk food as it ishighly nutritious and serves as an ideal medium for bacterial growth. The aim of this study was to monitorE. coli O157 contamination in the storage tank before distribution in Sleman district, Yogyakarta. Totalof 30 raw milk samples were collected from the storage tank in Sleman district. Tryptic Soy Broth (TSB)media added with novobiocin was used as enrichment medium, while Chromocult Coliform Agar (CCA)and Chromagar O157 medium for screening test. Additional analysis including serologic and moleculartest of isolates obtained. Based on the screening result, 11,428 colonies were considered as E. coli O157 suspectthat produced red colour in CCA medium. Further screening employing Chromagar O157 mediumresulted in 3 potential colonies which produce mauve colour. These colonies were later tested with LatexTest O157 for serological reason, showing that none were E. coli O157. Molecular analysis with primer pairsfor detection of Stx1 and Stx2 genes confirm that none of the suspected strains have genes that encodedthe toxin, Stx1 and Stx2. These results showed that the presence of STEC (Shiga toxin E. coli) hasn’t foundin the tested samples of raw cow’s milk.Keywords: E. coli O157, raw cow’s milk, storage tank, PCR
THE EFFECT OF RED SWEET POTATO (Ipomoea batatas L.) SUBSTITUTION ON SKIM MILK AS PREBIOTIC ON SYNBIOTIC DRINK POWDER CHARACTERISTIC Edhi Nurhartadi, Ratih Nawangwulan, Rohula Utami,
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

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Abstract

Red sweet potato (Ipomoea batatas L.) is known as a source of prebiotic because it contains oligosaccharideraffinose. Usage of prebiotic along with probiotic known as synbiotic. Powdered fermented synbioticdrink is one of the fermented drink diversification. The aim of this research was to study the effect of formulationratio of red sweet potato filtrate (RSPF) and skim milk (SM) on probiotic bacterial count, physicochemicaland sensory characteristic of synbiotic drink powder that dried using spray dryer.Formulation ratio of RSPF:SM = 75%:25% (F1), 50%:50% (F2), and 25%:75% (F3). Formulation ratio of RSPFand SM showed effect on pH, microencapsulation efficiency, solubility, and sensory properties. Lowest pHvalue (4.55), highest microencapsulation efficiency (4.47%) obtained from the formulation of 75% RSPFand 25% SM. Highest solubility obtained from the formulation of 50% RSPF and 50% SM. Based on sensoryanalysis, formulation of 75% RSPF and 25% SM had the highest sensory properties with ‘neutral’aroma (3.20); ‘neutral’ flavor (3.3); and ‘like’ overall (3.50).Keywords: fermented drink, raffinose, red sweet potato, spray drying, synbiotic
SCREENING OF SOYBEAN GERMPLASM COLLECTION RESISTANCE TO RUST DISEASE (Phakopsora pachyrhizi) -, Sumartini
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

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Abstract

Rust disease is an important disease on soybean, it was widely distributed in almost all soybeanproducing countries, the yield losses can be reach 85 %. One of the control measured is planting theresistant varieties. Resistant gens of one character can be obtained from the germplasm collection. Thestudy aiar to evaluate the resistance of soybean germplasm collection against rust diseases. The study wasconducted at Kendalpayak experimental station, Indonesian Legumes and Tuber Crops ResearchInstitute, in June to September 2013. Materials used were 175 genotypes of soybean germplasm collection.Observations of rust disease resistance based on the method of International Working Group on SoybeanRust system. The results showed that out of 175 soybean genotypes tested, 162 genotypes were moderatelyresistant, 12 genotypes were moderately susceptible , and one genotype was susceptible to rust disease.Keywords: soybean , rust disease, resistance.
TOTAL PHENOLIC CONTENTS AND ANTIOXIDANT ACTIVITIES OF TEN SOYBEAN PROMISING LINES TOLERANT TO ACID SOIL Heru Kuswantoro, Eriyanto Yusnawan, Alfi Inayati &
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
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Abstract

Marginal land such as acid soil has been recently used to cultivate soybean since soybean cultivated areain Java has shifted to other valuable crops. This expansion aims to fulfill national soybean demand and toreduce soybean import as well as to ensure soybean security. Ten soybean promising lines tolerant to acidsoil have been bred to address this issue. Secondary metabolites in soybean, i.e. phenolic compounds varyin each genotype. These compounds function mainly as chemical defense against insects and pathogens.Also, the phenolic compounds including flavonoids which hypothetically have antioxidant activities havepositive effects to human health. Phenolic contents of ten soybean promising lines ranged from 2.87 to3.73 mg GAE/g. Five varieties, i.e. Wilis, Tanggamus, Anjasmoro, Grobogan, and Argomulyo as the checkshad the phenolic contents of 3.02 to 3.93 mg GAE/g. Flavonoid contents of the ten lines varied from 0.57to 0.76 mg CAE/g. There were no check varieties which had the flavonoid contents higher than those ofthe promising lines. The Tgm/Anj‐784, Tgm/Anj‐856, Tgm/Anj‐844, and Tgm/Anj‐857 had the equallyhigh flavonoid contents, i.e. 0.76, 0.75, 0.72, and 0.72 mg CAE/g, respectively. Antioxidant activities as indicatedby DPPH values of the ten soybean lines were lower than those of Wilis, Argomulyo, andGrobogan.Keywords: soybean promising line, acid soil, phenolic content, flavonoid content, antioxidant activity.
CHEMICAL AND FREE FATTY ACID COMPOSITION OF GOAT MILK CHEESE RIPENED WITH Lactobacillus acidophilus AND EXTRACT RABBIT STOMACH AS COAGULANT Indratiningsih, Nurliyani, Sulvia Dwi Astuti &
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
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Abstract

Rabbit stomach is waste from rabbit slaughtering that has the potential to be used as an alternativesource of rennet for cheese production. The purpose of this study was to determine the effect of ripeningby using Lactobacillus acidophilus on the chemical composition and free fatty acids (FFA) profile ofgoat milk cheese with extract of rabbit stomach as coagulant. Lactobacillus acidophilus (1%) was inoculatedinto pasteurized goat milk and incubated for 1 h, rennet extract up to 3% was added and incubatedfor 25 min. Cheese was stored at 4οC for 42 d. The moisture, fat, protein and ash content wereanalyzed at day 0, 21 and 42, whereas the FFA of goat milk cheese were analyzed on day 0 and 42. Therewere significantly differences in the moisture, protein, and ash content in the goat milk cheese on 21 and42 days of ripening, but there was no significant difference in the fat content. The long‐chain saturated(C16:0 and C18:0) and unsaturated (C18:2) FFA were the most abundant in goat milk cheese, and therewere no significant differences between pre or post ripening. The concentration of short chain fattyacid (C6:0, C8:0 and C10:0) and the overall concentration of FFA in goat milk cheese after 42 days ripeningwas higher compared to those pre‐ripening.Keywords: Chemical composition, Free fatty acid, Goat milk cheese, Rabbit stomach
CHARACTERISTICS OF KWETIAU MATERIAL OF FORMULATION RICE FLOUR AND UWI FLOUR, TARO FLOUR AND KIMPUL FLOUR MODIFIED BY HEAT MOISTURE TREATMENT (HMT) Ledy Purwandani, Erning Indrastuti, Muflihah Ramadhia &
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
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Abstract

The purpose of this study is to determine the effect of substitution uwi flour, taro flour, and kimpul flourmodified on rice flour that can produce the best characteristics of high resistant starch kwetiau. HMTmodification on the uwi/ keribang/ coconut yam (Dioscorea alata) flour, taro (Colocasia esculenta (L)Schott) flour and kimpul/ sarawak taro/ belitung taro (Xanthosoma sagittifolium (L) Schott) flourcharacteristics can be applied to the manufacture of kwetiau . The nature of the lack of appropriatenatural starch is expected to be improved by modification of starch is by Heat Moisture Treatment(HMT). HMT treatment is 20% and 30% water content with heating time 4, 6, 8, 10 hours at 80 ° C. Theresults showed HMT modification effect on the water content and crystallinity of uwi flour, taro flour andkimpul flour.Keywords: Heat MoistureTreatment, Kimpul, Resistant Starch, Taro, Uwi.
APPLICATION OF BIOTHECHNOLOGY AGRICULTURAL BY MANURE, EFFORT OF SESAME PRODUCTION IN COASTAL SANDY LAND Taryono & Eko Hannudin, Dewi Ratna Nurhayati, Yudhono, Prapto,
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
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Abstract

Coastal sandy land as one of marginal land which potential become to be a sustainable productiveland. Annual crops such as Sesame cultivation proper to developed as an agribusiness crops in coastalsandy land. Integrated fertilizer model ( by manure, or organic fertilizer and in organic fertilizer )Manurefertilizer, is one of Biotechnology in agricultures products. This study aimed to know the growth andyield of Sesame (Sesamum indicum L.) in quantity and quality through the combination treatment of manureand inorganic proportionately which refers to the dose and type of manure elected. Phase II studyconducted in a polybag, for planting. This study conducted in Banguntapan Yogyakarta, Agricultural stationlab. from September ‐ December 2012 aimed to determine the proportion of fertilizer best combinationof sesame results based on the dose of manure was selected from the results of the Phase I study(chicken manure 30 t / ha).This study used a factorial experiment (7 X 2) were prepared by completely randomized design (CRD)with repetition three times. Experiment arranged in factorial with two‐factor, repeated three times. Firstfactor is combination of manure and inorganic fertilizer (K), there are 7 types: (Sandy Land media: K0 1.Chicken manure 30 t / ha: K1, 2. Chicken manure 15 tonnes / ha (100% recommendation farmers) + inorganicfertilizers (N, P, K deficiency is equivalent to substitute 15 tonnes / ha of chicken manure): K2, 3.chicken manure 11.25 tonnes / ha (75% of farmers recommendation) + inorganic fertilizers (N, P, K deficiencyreplacement dose equivalent to 18.75 tonnes / ha of cow manure): K3 4. chicken manure 7.5 t / ha(50% of farmers recommendation) + inorganic fertilizers (N, P, K deficiency replacement dose equivalentto 22.5 tonnes / ha of chicken manure): K4 ,5. chicken manure 3.75 t / ha (25% of farmers recommendation)+ inorganic fertilizers (N, P, K deficiency replacement dose equivalent to 26.25 tonnes / ha of chickenmanure): K5 ,6. inorganic fertilizers (N, P, K deficiency replacement dose is equivalent to 30 tons / ha ofchicken manure): K6.Second factor is the variety: Sumberrejo‐1, Sumberrejo‐2, Variable observed were:plant height, days to flowering, day of harvest, seed weight per plant, weight of 1000 seeds, and seed oilcontent. The data analyzed by Anova 5%, with further testing Duncan 5%. The results showed The fastestflowering (45 days) was on the control treatment. The age of harvest is almost the same, 105 days. Highesttotal seed oil content, 42.72%, obtained in the treatment of chicken manure 7.5 t / ha (50% of farmers recommendation)+ inorganic fertilizers (N, P, K deficiency replacement dose equivalent to 22.5 tonnes / haof chicken manure) in Sumberrejo‐1Keywords: Coastal sandy land, organic fertilizer, in organic, Sumberrejo‐1, Sumberrejo‐2 Sesame(Sesamum indicum L.)
ECOLOGICAL ANALYSIS OF UNDERSTORY VEGETATION AROUND ENDANGERED ORCHID PENCIL (Vanda hookeriana Rchb.) FROM LAKE DUSUN BESAR NATURE RESERVE BENGKULU Roziaty, Efri
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
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Abstract

The research on Ecological Analysis Of Understory Vegetation Around Endangered Orchid Pencil (VandaHookeriana Rchb.) From Danau Dusun Besar (DDS) Nature Reserve Bengkulu held on February to December2013. The research is located around endangered Pencil orchid in DDS Bengkulu Province. Pencilorchid is an endangered orchid in the world. It’s an endemic orchid from DDS Bengkulu Province. Theresearch used purpossive sampling techniques in site selection and using 2 m x 2 m = 4 m2 plot for sampling.The highest Important Value Index (INP) value is Imperata sp. (Graminae) 78.60 %. Some ecologicalfactors which influenced the vegetation such as temperature, humidity, sunlight penetration and the pHof the water. The researcher stated that the assessment needs to be done about the ecological aspects oforchid pencil in their natural habitat.Key words : Danau Dusun Besar Bengkulu, understory vegetation, endemic orchid.

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