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Y. Andi Trisyono
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anditrisyono@ugm.ac.id
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jpti.faperta@ugm.ac.id
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 10, No 1 (2004)" : 7 Documents clear
Perbaikan Kualitas Semai Tusam Pasca Sapih melalui Aplikasi Trichoderma Formulasi Pupuk Lambat Tersedia dan Substitusi Media Tumbuh S. M. Widyastuti; Sumardi Sumardi; N. Estikasari
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12210

Abstract

The experiment was conducted based on damage developed on pine nursery, which killed pine seedlings at a significant number. The damage started on 4 month-aged seedlings indicated by yellowing of needle, followed by development of brown spots on the needle and subsequently could kill the transplanted seedlings. Application of formulated Trichoderma and slow released fertilizer was aimed to avoid the initial symptom, which was assumed due to nutrient deficiency and to control the subsequent infection and disease development. Substitution of composting materials and rice hull into regular growth medium as the main treatment resulted 4 combination treatments. The results indicated that despite all treatments showed significant effects, application of Trichoderma together with slow released fertilizer (PJTJ) on regular medium (medium IV) gave the highest growth increment performance and suppression of damage in seedlings.
Daya Tahan Hidup Pseudomonas putida Strain Pf-20 dalam Beberapa Macam Inokulum Yenny Wuryandari; Triwidodo Arwiyanto; Bambang Hadisutrisno; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12211

Abstract

For any crop-protection agent, an efficient formulation is a necessity to translate laboratory activity into adequate field performance. There are particular challenges to be faced in formulation of biological control agents, because the active ingredient is a living organism that must be kept relatively immobile and inactive while in storage, but quickly resume its  normal metabolic processes once applied to the target site. The objective of the research was  to study survival of Pseudomonas putida strain Pf-20 in various formulations at the storage  time and germination. The twelve formulations include carriers, additives and concentration  of Pf-20. The efficacy of various formulation in maintaining the population of Pf-20 in storage was assessed. The research result showed that population of Pf 20  in the formulation  number seven was the highest, with the combination peat+talc, CMC+arginin and  concentration of Pf[20 10^10 CFU/ml. In peat+talc, CMC+arginin, Pf-20 10^10  CFU/ml based formulation the bacteria survived even up to 84 days of storage although the population declined. In all formulations, population of Pf-20 increased at the time of seed germination. At the time of seed germination, formulation number seven was the highest too.
Dampak Pengendalian Hama Terpadu terhadap Pendaftaran dan Penggunaan Pestisida di Indonesia Kasumbogo Untung
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12206

Abstract

Integrated Pest Management (IPM) legally has been adopted as the principle of crop protection practices in Indonesia since 1992 through the release of Law No.12 /1992 on Crop Husbandry System and Government Regulation No.6/1995 on Crop Protection. Data was collected from the national registration authority and the national statistic agency for evaluating the impact of IPM policy to the registration and use of pesticides by farmers. The data showed that the number of registered pesticide formulations, and the production and distribution of pesticides have increased since 1989. The number of formulations reached the peak in 2002 with a total of 813. On the other hand, the production was most in 1996 (80,000 ton) and the highest use of pesticide by rice farmer occurred in 1991 (4,72 kg pesticide/ha). Most of present registered pesticide formulations are generic. The data indicated that nationally IPM has not been significantly altered the registration, distribution and uses of pesticides by farmers in Indonesia. Comprehensive and integrated efforts must be established and implemented seriously by all stakeholders to institutionalize and implement IPM.
Pembuatan Antiserum dan Kajian Serologi Virus Penyebab Penyakit Daun Keriting Kuning Cabai Sri Sulandari; Rusmilah Suseno; Sri Hendrastuti Hidayat; Soemartono Sosromarsono; Jumanto Harjosudarmo
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12213

Abstract

Virus identification based on the serological assay has been widely applied as a tool for plant virus detection. The aims of this research is to produce antiserum of the Pepper yellow leaf curl virus by rabbit immunization using purified gcminivirus of Segunung isolate. Identification of the virus was done by using modified I-ELISA and DIBA methods and also by using western blott. I-ELISA and DIBA methods were able to detect the geminivirus in the infected samples. The reactivity of antiserum was found to be similar amontI pepper isolates from different location (Segunung, Yogyakarta, Cugenang, and Lembang) ana those from different hosts (pepper, tobacco, tomato and Ageratum conyzoides) The antiserum could also be used for detection and identification of the Pepper yellow leaf curl virus in its vector. A single insect vector is sufficient for the detection of virus properly. The detection of geminivirus in its vector is very useful because it can be used to study the epidemic of the disease in the field. The I-ELISA and DIBA methods are very useful as tools for detecting the geminivirus. The methods are very easy to be carried out, fastly, and need only a minimum cost on operation. Geminivirus could also be identified by western blott analysis.
Patogenisitas Beberapa Isolat Beauveria Bassiana pada Plutella xylostella Suharto Suharto
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12207

Abstract

Plutella xylostella (L.) (Lepidoptera : Plutellidae) is key pest on cabbage. P. xylostella can be infected by Beauveria bassiana from other insects. The objective of this study was to determine the pathogenicity five isolates of B. bassiana on P. xylostella. Those isolates were BbUj1 (larvae of Lepidoptera), BbUj2 (rice brown plant hopper), BbUj3 (rice brown plant hopper), BbUj4 (rice seed bug), and BbUj5 (rice black bug). In the laboratory every isolate was inoculated 5 µL spore suspension at rate 107 spores/mL to third instar P. xylostella larva used micro liter syringe. Each treatment used 10 larvae and was replicated five times. The pathogenicity of B. bassiana was determined based on mortality and LT50 value. The potential isolate was tested to find LC50 subsequently tested on cabbage crop in the green house. The result of the study showed that BbUj1 from larvae of Lepidoptera was the potential isolate with LC50 of 9.49 x 10^5 spores/mL. LT50 was 2.67 days for the concentration 107 spores/mL. Green house application of B. bassiana at rate of 5 x 10^12 spores/ha caused 57 percent mortality of P. xylostella larva in seven days after treatment and LT50 was 5.67 days.
Bioactivity of A Bacillus Thuringiensis Cry1AC Toxin to Spodoptera litura Y. Andi Trisyono; Serafina T.S. Rahayu; St. Margino
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12214

Abstract

The transgenic cotton expressing Bacillus thuringiensis toxin Cry1Ac in Indonesia has been planted since 2000 for controlling Helicoverpa armigera. Spodoptera litura is another lepidopteran insect that also attacks cotton. The objective of this research was to determine the toxicity of Cry1Ac to S. litura. The acute toxicity was determined using neonates of S. litura exposed to artificial diets treated with series concentrations of Cry1Ac ranging from 0.14 to 625 mg/ml, and larval mortality was recorded at the seventh day after treatment. The chronic toxicity was determined by exposing neonate to artificial diets treated with the sublethal concentrations (LC5 and LC40). The growth and development of treated larvae were compared with those of the control farvae. Cry1Ac was toxic to neonate with LC5 values of 71.9, 18.1, 24.7, and 16.2 mg/ml for S. litura collected from Wonosari, Bantul-l, Bantul-2, and Kopeng, respectively. Cry1Ac was more toxic than formulated B. thuringiensis (LC50 = 724.8 mg/ml). Larvae exposed continuously to artificial diets treated with sublethal concentrations of Cry1Ac (0.61 and 9.77 mg/ml) showed no significant difference on weight and length of each life stages than those of the control larvae. These indicate that the application of the tested sublethal concentrations of Cry I Ac did not affect the growth and development of S. litura. However, increasing concentrations of Cry1Ac (156.25 and 625 mg/ml) significantly reduced the weight of surviving larvae. These findings suggest that application of Cry1Ac to S. litura shows some toxicological effects. The effectiveness of the transgenic cotton in controlling S. litura in field situation will be discussed.
Aktivitas Insektisida Ekstrak Kulit Batang Empat Famili Tumbuhan terhadap Ulat Krop Kubis Crocidolomia pavonana (F.) Edy Syahputra; Djoko Prijono; Dadang Dadang; Syafrida Manuwoto; Latifah K Darusman
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12208

Abstract

The objective of this study was to evaluate the insecticidal activity of bark extracts of 35 plant species belonging to four families (Clusiaceae, Lecythidaceae Meliaceae, and Sapindaceae) against the cabbage head caterpillar Crocidolomia pavonana. Extraction of the test plant materials were performed with maceration method using ethanol and methanol continued by counter-current distribution separation in ethylacetate and water. Bark extract preparation of each plant species was tested against C. pavonana larvae by leaf-feeding method at concentration of 0.25% and 0.5%. Second-instar C. pavonana larvae were fed extract-treated broccoli leaves for 48 hours, then were presented with untreated leaves until the surviving larvae reached the fourth-instar larvae. The number of dead larvae was recorded. The phytochemical test of bark extract of Calophyllum soulattri was done by qualitative method. The results showed that the bark extracts of C. soulattri (Clusiaceae), Barringtonia sarcostachys (Lecythidaceae), and Aglaia tomentosa (Meliaceae) exhibited good insecticidal activity. Methanol extract of C. soulattri barks possessed strong insecticidal activity against C. pavonana larvae with LC95 of 0.21%. This extract showed positive response in alkaloid and triterpenoid qualitative tests. Further studies are needed to identify insecticidal compounds in those active extracts.

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