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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol 24, No 2 (2020)" : 15 Documents clear
Appropriate Number of Sex Pheromone Trap for Monitoring Spodoptera exigua Hubner (Lepidoptera: Noctuidae) Moths on Shallot Field Dian Lestari; F. X. Wagiman; Edhi Martono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.23370

Abstract

The research was carried out to determine the appropriate number of sex pheromone traps for monitoring the Spodoptera exigua moths in shallot crop which was grown in Yogyakarta Special Territory, Bantul Regency (Samas). This research was conducted in August until September 2016. Sex pheromone trap was arranged on shallot aged  3 weeks after planting. Sex pheromone trap with hexadecenat tetra asetat ingredients was placed on sticky yellow trap. This research  used RCBD experimental design with single factor experiment namely the number of trap i.e. 3, 5, 6, 8 units/2.000 m2 with sex pheromone, and  three traps without sex pheromone as control. Each treatment was repeated 5 times so  125 samples of experiments were obtained. Observation was made by counting the number of moths caught in sex pheromone trap. The result of observation was analysed  with analysis of variance (ANOVA) and DMRT α 5.0%. The results show that the appropriate number of trap to monitor the pest infestation was 3 units/2.000 m2.
Responses of Capsicum annuum Varieties toward Root Knot Nematode Meloidogyne incognita Infection Resty Islamiati Putri; Siwi Indarti; Ani Widiastuti
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.23978

Abstract

Chili pepper (Capsicum annuum) is one of the major vegetable commodities in Indonesia. One of the challenges in chili pepper cultivation is the infection of Meloidogyne incognita which causes gall formation in root systems. The purposes of this research were to determine 1. the response of four varieties of chili pepper against M. incognita infections; 2. damaged intensity caused by this pest in the root system, and; 3. development of M. incognita populations in root systems (rhizosphere). This research was conducted in Plant Pest Science Laboratory and Greenhouse of the Department of Crop Protection, Faculty of Agriculture, University of Gadjah Mada. This research included cultivation of three chili pepper varieties (Cabai Merah Besar, Pilar F1; Cabai Merah Keriting, Kastilo F1; and Paprika, Red Star 2060), rearing of M. incognita, inoculation, and observation. Chili pepper respond to M. incognita infections were evaluated based on agronomic aspects of the plants, such as root histopathology observation, disease intensity, and nematode populations. Results showed that each variety of chili pepper expressed various responses to infections. All varieties had different root weight and length, but had similar sensitivity based on the appearance of root damage and egg mass scoring of M. incognita. Although nematode population could develop on roots, observation from root tissues showed lignification after infection of J2 M. incognita. Chili pepper var. Red Star 2060 was more susceptible against M. incognita than chili pepper var. Pilar F1 and chili pepper var. Kastilo F1.
Molecular Identification of Begomovirus Infecting Angled Luffa Alvina Clara Giovanni; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.31073

Abstract

Begomovirus was reported as one of the most aggressive and destructive viruses on several commercial crops, including cucurbits in Indonesia. Plants that infected with Begomovirus show the mosaic symptom on the leaves, change in leaf shape, stunts, change in color and shape of fruit. It was recently observed in cultivated angled luffa [Luffa acutangula (L.) Roxb] around Yogyakarta and Central Java. The aim of this research was to identify the virus by using Polymerase chain reaction (PCR). The result of Begomovirus amplification from the total DNA samples amplification using primer Krusty-Homer showed that DNA of Begomovirus from angled luffa was amplified at ~580bp. The DNA sequencing of angled luffa’s leaf isolate GD1 had 97.8% homology with SCLV-China isolate MC1. However, amplification of DNA seed samples using the same primer showed negative result. It was concluded that Begomovirus was not a seed borne virus. This is the first molecular report on the occurence of Begomovirus in angled luffa in Yogyakarta.
Weed Seed Species Inventory of Wheat Imported through Tanjung Perak Port Ulya Wildaniyah; Rahma Susila Handayani
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42159

Abstract

Wheat seeds importation has the potential of weed seeds that can harm Indonesia's biodiversity. This study aims to determine the types and morphological characters of weed seeds carried by imported wheat from Australia, Canada, and Ukraine through the port of Tanjung Perak-Surabaya for January-June 2018. The research was started by collecting weed seeds from wheat samples, observing using a SMZ 1270 stereo microscope and identifying them using a reference book. The weed seeds were successfully identified as 13 families with 20 species originating from Australia, Canada and Ukraine. The most common types of weeds are from the Poaceae family. Weed this family are often found in the form of florets, the seeds of which are called caryopsis are coated in lema and palea, as in grains. Weed seed species found in wheat commodities from the three countries, are: Avena fatua, Brassica campestris, and Polygonum convolvulus, are not classified as quarantine pest.
Feeding Inhibition by Chitosan on Larvae of Spodoptera litura (Lepidoptera: Noctuidae) Sulistia Ningsih; Nugroho Susetya Putra; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.44032

Abstract

The high usage of synthetic insecticides for controlling Spodoptera litura could be detrimental to the environment, especially on non-target organisms. Therefore, more environmentally friendly pest management techniques should be used, for example, using a natural product such as chitosan. The objective of this study was to understand the effect of feeding inhibition by chitosan on the feeding activity of the third instar larvae of S. litura. The feeding inhibition test was carried out using the choice methods in a factorial completely randomized design (CRD) with seven treatments: i.e. chitosan 5×103, 15×103, 25×103, 35×103, 45×103 ppm and profenofos 0.18×103, 0.34×103 ppm, and one control treatment. The test of the choice methods was carried out by three larval laying positions: (1) between the control and treatment feeds (Position A), (2) above the treated feed (Position B), and (3) above the control feed (Position C), and were replicated three times. The non-choice test was done in a completely randomized design (CRD) with seven treatments plus control and were replicated four times. The results showed that the chitosan in the concentration range of 5×103‒45×103 ppm reduced feeding by S. litura larvae by 2.587 to 34.974% in the choice method, and 11.610 to 50,712% in the non-choice method. This feeding inhibition increased significantly with the increment of chitosan concentration. However, the inhibition effects by chitosan was weaker than the inhibition by profenofos LC50 in both tests at a concentration of 0.34×103 ppm: 44.331% and 62.491% respectively. In conclusion, chitosan with a concentration of 45×103 ppm at all larval laying positions showed the highest value of feeding inhibition activity on the third instar larvae of S. litura compared to other chitosan concentrations in both methods.
The Role of Nanochitosan on the Expression of Rice Resistance Genes against Bacterial Leaf Blight Siswanti Siswanti; Tri Joko; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.44418

Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has been reported to cause ± 20–50% of rice yield loss around the world. Resistant varieties are used to control this disease, however due to rapid evolution of this pathogen, the resistances was broken down in a few years. This study is aimed to determine the role of nanochitosan in the expression of rice Xa21 and Xa1 resistant genes against Xoo. The BLB susceptible rice cultivar IR64, the Xoo isolate MAG2 and a 0.065% concentration of nanochitosan were used in this experiment. Application of nanochitosan was carried out within 1-week intervals starting at rice aged 2–10 weeks after transplanting. The expression of Xa21 and Xa1 genes against Xoo were analyzed using conventional PCR and qPCR methods at 0 and 4 days after Xoo inoculation followed by 4x scoring of disease symptoms in 1-week interval. The treatments used in this study included the mock one/inoculated with sterile distilled water, K (+)/ plants inoculated with Xoo, CNP (-)/ with nanochitosan and sterile distilled water inoculation, and CNP (+)/ with nanochitosan and Xoo inoculation. The results showed that the 0.0065% concentration nanochitosan application was able to increase the expression of Xa21 and Xa1 genes on CNP (-). Disease intensity and AUDPC values did not show any significant difference between K (+) and CNP (+). This study concluded that nanochitosan at 0.065% was able to increase the expression of rice Xa21 and Xa1 resistance genes. However, the gene expression was not able to significantly suppress the infection development of Xoo.
History of Sheath Rot Disease in Indonesia and Disease Severity in Two Rice Production Centres of West Java Khoirotul Afifah; Suryo Wiyono; Titiek Siti Yuliani; Baskoro Sugeng Wibowo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47665

Abstract

The observation conducted from 2000 to 2018 found that rice sheath rot has become an emerging disease in several regions. The disease was able to reduce quality and quantity of rice yield. This research objective was to study the history and status of rice sheath rot caused by Sarocladium oryzae. Data was obtained from literature study, farmer interview, and field survey. Data were used to calculate disease incidence followed by visual observation of the symptoms caused by this disease. Identification of sheath rot pathogen was done using microscopic and molecular techniques using specific primers. Rice sheath rot was first reported in Indonesia as a minor disease in 1987 and has only currently become an important disease. This fungus generally causes rice sheath rot in Karawang and Cianjur Regency. Disease incidence of rice sheath rot was 12.56% without considering the varieties at all generative growth stages. Average disease incidence on all rice varieties observed was 12.64%, except for IR 42 (0%). The level of rice plants damage due to rice sheath rot in Cianjur was higher than Karawang with average disease incidence of 11.58% and 9.27%, respectively. Rice sheath rot symptoms have often been found in the last 10 years with average level of damage of 3%‒18% and yield loss of 10%-15%. This disease has proven to be important and has become a challenge in rice cultivation. The fungus that infect rice plants in Karawang and Cianjur had different morphotypes, namely KP, KP2, KP3, PW3, and PW03. The amplification results showed that all fungal isolates were S. oryzae.
Inhibition of Rehmannia mosaic virus Infection by Ganoderma sp. Extract Mustika Ajeng Kartini Putri Pertiwi; Suryanti Suryanti; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.49592

Abstract

In fruiting bodies and mycelia of several fungi belonging to a Basidiomycetes group, among others, Ganoderma contains active polysaccharides and has potential as an antiviral substances. This study aimed to determine the effect of mycelium and fruiting body extract of Ganoderma sp. against Rehmannia mosaic virus (ReMV) infections on Chenopodium amaranticolor with variations of dilution and application times. The results showed that mycelium and fruiting body extract of  Ganoderma sp. could inhibit ReMV infections. Antiviral extract which were applied 4 hours before, and at the same time with virus inoculation resulted in a higher viral inhibition rates than when applied 4 hours after virus inoculation. Fruiting body extract at 10-1 and 10-2 dilutions delayed symptom appearance caused by ReMV infection when applied 4 hours before virus inoculation. Mycelium extract at dilution of 10-2 and 10-3 applied 4 hours before virus inoculation resulted in the highest inhibition rate (100%) which was indicated as the number of local lesions on Chenopodium amaranticolor leaves. Meanwhile the fruiting body extract dilution of 10-1 resulted in highest inhibition (99.55%) when applied 4 hours before virus inoculation.
Phytonematodes Community and Polyphasic Character of Aphelenchoides varicaudatus on Garlic Plants in Tegal Regency, Central Java Mokhammad Danang Kusuma; Supramana Supramana; Giyanto Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.49779

Abstract

The interception of Ditylenchus dipsaci, D. destructor, and Aphelenchoides fragariae, which are classified as quarantine pests, has been reported to occur on imported garlic bulbs used for consumption at several traditional markets in Bogor. This finding should increase awareness of the chance of garlic contamination in Indonesia’s garlic fields. This study aimed to evaluate the nematode community and determine polyphasic characters of Aphelenchoides species from garlic plantations. The study was conducted by sampling soil and plants from garlic plantations in Tuwel and Rembul Villages, Bojong District, Tegal Regency, Central Java in December 2018. Nematode extraction was done using a mist chamber for plant materials and sugar flotation – centrifugation methods for soil samples. Parameters measured included nematode species identity, absolute population and prominence indexes. Nematode identification was conducted based on their morphological characters. Further identification based on morphometric and molecular characters (polyphasic) conducted for nematodes species suspected as quarantine pests. Six generas of nematodes identified were Helicotylenchus, Aphelenchoides, Rotylenchulus, Aphelenchus, Criconemoides, and Tylenchus. Aphelenchoides sp. had the highest prominence value from plant tissue with a value of 6.32 and is categorized as a quarantine pest genus (A. fragariae) in garlic and was further identified to the species level. Further identification based on polyphasic characters showed the Aphelenchoides sp. found was A. varicaudatus.
Oviposition Preference of Bactrocera dorsalis Hendel (Diptera: Tephritidae) on Different Fruit in Snake Fruit Orchard Rahmi Fitrah; Deni Pranowo; Suputa Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.52825

Abstract

The oriental fruit fly Bactocera dorsalis (Hendel) is an important pest of snake fruit (Salacca zalacca) in Sleman District. Due to the high level of damage by the fruit flies, it is necessary to do. The aim of this research was to find out suitable fruit traps, by testing the oviposition preferences of the fruit flies in the orchard to lay eggs on several types of fruit. The research was done in snake fruit orchard located at Sleman Yogyakarta and owned by farmers, while the Lab works were done at the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. All researches were done between April-June 2019. Guava (Psidium guajava), watery rose apples (Syzygium aqueum), starfruit (Averrhoa carambolae), and snake fruit (Salacca zalacca) were used as trap crops. Each fruit, with the same maturity level, was hung 1.5 m above the ground for 4 days with 12 days total trapping at intervals of 3 times. After the test, each fruit was taken and the insects in it were reared in the laboratory. The number of pupae and flies that emerged from each fruit was counted and compared. The results showed that in the orchard 1 as well as  2, of guava fruit produced the highest number of (151 pupae) followed by salak (94 pupae), star fruit (83 pupae), and water guava (2 pupae). The finding of seeds shows that guava fruit is the most suitable host for the fruit flies to be used in the trapping, followed by star fruit and watery guava.

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