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Jurnal Perlindungan Tanaman Indonesia

jpti
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Published by Universitas Gadjah Mada

ISSN : 14101637 EISSN : 25484788 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Arjuna Subject : -

Articles 509 Documents

7 8 9 10 11

Struktur Komunitas Artropoda pada Ekosistem Padi Tanpa Perlakuan Pestisida Eddy Mahrub
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 1 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9836

Generally, rice ecosystem was les stable because the structure of natural community often change, and it may cause decrease in the natural biodiversity. The research has been done in the lowland ricefield during wet and dry season without pesticides application. Observation was started when the plant (IR64) was 7 days old after transplanting with interval of 8 days until one week before harvest, to collect the arthropods from each samples. The objectives were to study the structure of community of arthropods during wet and dry season and to study food web chain in natural ecosystem and its implementation in the Integrated Pest Management Program. The results showed that in rice ecosystem four arthropod groups or guilds, was found i.e. Pests, Predators, Parasites and Neutral Arthropods. The diversity and species abundance of arthropods were high but the highest abundance of arthropods was found on the member of certain order and family only. The population of pest was lower than predator. The relative abundance of pests were 5.75% in wet season and 8.57% in dry season, while predators were 27.96% in wet season and 33.88% in dry season. The population density of spider predators especially Lycosa sp. during wet and dry season were very high. It was supported by the abundant of neutral arthropods as alternate prey when the pest population was very low. This condition may support negative feed back interaction and increase the potential of natural control in the absence of pesticide application.

Optimasi Produksi Endokitinase dari Jamur Mikoparasit Trichoderma reesei Harjono Harjono; Siti Muslimah Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 1 (2001)
Publisher : Universitas Gadjah Mada

The objective of the experiment was to select optimal medium composition to induce endochitinase production of Trichoderma reesei. Growing media were prepared by substitution of 0.5% colloidal chitin, 0.5% colloidal chitin and 1% Polyvinyl Pyrrolidon (PVP), 1% crab shell chitin (Sigma), 1% crab shell chitin and 1% PVP into Richard’sMedium (RM). Selected bioagent T. reesei isolate 13 was grown onto the media and the specific activity of endochitinase was measured. The results showed that T. reesei grown in all media performed enzyme expression. The highest specific activity of endochitinase was detected on T. reesei grown in RM medium substituted with colloidal chitin and PVP, followed by the same fungi in RM medium substituted with crab shell chitin plus PVP, colloidal chitin and crab shell chitin alone.

Konsentrasi PEG 6000 dan Senyawa Aditif Buffer Fosfat yang Diperlukan dalam Pemurnian Soybean Mosaic Virus Y. B. Sumardiyono; Susamto Somowiyarjo; Wuye Ria Andayani
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

The objective of study was to determine the concentration of PEG 6000 for precipitation of virus particles, and additive substance added to the resuspension buffer, during the purification of Soybean Mosaic Virus isolated from Yogyakarta. The result showed that precipitation with PEG 6000 of 6 per cent at final concentration, followed by either two or three cycles differential centrifugation and the virus obatained from each centrifugation resuspended in Phosphate buffer 0.05 M contained NaCl 0.1 M added with 0,005 M Na-EDTA, and then subjected to 10-50 per cent sucrose density gradient centrifugation. The purified virus obtained was infective, with the ultraviolet absorption maximum at 260 nm and minimum at 247 nm, ratio A280/A260 was 0.7343. based on extinction coefficient at 260 nm = 2.4, the yield was 0.306 mg/100 g infected leaves. SDS-PAGE indicating that coat protein molecule weight was 29.71 kD.

Optimasi Metode PCR untuk Deteksi Pectobacterium carotovorum, Penyebab Penyakit Busuk Lunak Anggrek Tri Joko; Nanda Kusumandari; Sedyo Hartono
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Soft rot is one of the most important diseases of orchids caused by Pectobacterium carotovorum. The conventional methods for the detection of pathogen is tedious and time consuming. In recent years, numerous molecular diagnostic approaches for the detection of P. carotovorum have been developed, including various PCR-based assays. Optimization of PCR technique to DNA amplification is essential for time and material efficiency, which will make detection to be rapid and more appropriate. The purposes of this study were to decide concentration of DNA and primer, and also the concentration of bacterial pure cultures and primer to amplify 16S rRNA gene fragment. Optimization of PCR was done by using various concentration of DNA, pure cultures of bacteria, and primer to amplify the 16S rRNA gene sequence. The results showed that the most optimum concentration to amplify 16S rRNA gene sequence at DNA and primer concentration were 63,4 ng/µl and 10 pmol, while pure cultures and primer concentrations were at 8×109 CF U/ml and 10 pmol respectively. Penyakit busuk lunak yang disebabkan oleh Pectobacterium carotovorum merupakan salah satu penyakit penting pada tanaman anggrek. Deteksi patogen secara cepat dan akurat dapat dilakukan secara molekular menggunakan teknik Polymerase chain reaction (PCR). Optimasi metode PCR perlu dilakukan untuk mengefisienkan waktu dan penggunaan bahan sehingga proses deteksi dapat dilakukan dengan cepat dan tepat. Penelitian ini bertujuan untuk menentukan konsentrasi DNA dengan primer maupun konsentrasi kultur murni bakteri dengan primer yang paling tepat untuk mendapatkan fragmen gen 16S rRNA. Optimasi PCR dilakukan menggunakan beberapa variasi pengenceran pada DNA, kultur murni bakteri, dan primer untuk mengamplifikasi gen 16S rRNA. Hasil penelitian menunjukkan bahwa konsentrasi yang paling optimal untuk mengamplifikasi gen 16S rRNA yaitu DNA dan primer masing-masing sebesar 63,4 ng/µl dan 10 pmol, sedangkan konsentrasi kultur murni dan primer sebesar 8×109 CFU/ml dan 10 pmol.

Pengendalian Hayati Penyakit Layu Bakteri Tembakau: 2. Percobaan di Rumah Kaca Triwidodo Arwiyanto; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 1 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9967

Tobacco bacterial wilt caused by Ralstonia solanacearum has been controlled biologically in the greenhouse by the use of rhizosphere microorganisms, i.e. fluorescent pseudomonads and Bacillus spp. Root dipping for 30 minutes in the antagonist suspension (108 cfu/ml) suppressed bacterial wilt development. The best results were obtained with fluorescent pseudomonads Pf-20 and Bacillus sp. ba-118 isolates but the repression was annulled with the use of its combination. Disease severity were higher when the plants were treated with the combination between fluorescent pseudomonads. Treatment combinations between Pf-28, Pf-31, Pf-33, and Ba-118 could reduce the disease index.

Jamur Upas (Upasia salmonicolor) pada Daun Kopi di Pagilaran Ambarwati Harsojo Tjokrosoedarmo
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

The result of this study showed that most of fungus as thin mycelial layer on the coffee leaves were connected with pink fungus on the branch. The microscopic study showed the hyaline mycelia producing perpendicular branches, and anastomose easily; the hyphal cells contain one to four nuclei. The anatomy of pink incrustation of fungus composed of four layers: 1. basal layer; 2. intermediate layer; 3. subhymenial layer; and 4. hymenial layer. From the results of both field and laboratory studies it is concluded that the fungus on the coffee leaf is actually the cobweb stage of pink fungus occurring on the leaves, and it is notorious fungus because it may act as inoculum source of pink disease.

Peran Infeksi Jamur dalam Memperparah Kerusakan Buah Kakao Akibat Serangan Helopeltis sp. Jurnal PTI Vol. 1 No. 1 Hal. 28-32 Teddy Suparno
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

Ulasan Balik Artikel (Tanpa Abstract)

Percobaan Pendahuluan Pengaruh Minyak Mimba dan Ekstrak Biji Srikaya terhadap Mortalitas Helopeltis sp. (Heteroptera: Miridae) Soekadar Wiryadiputra
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 2 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9906

Preliminary trial of neem (Azadirachta indica A. Juss.) seed oil and aqueous seed extract of custard-apple (Annona squamosa L.) on the mortality of Helopeltis sp. has been conducted in laboratory condition. Neem oil obtained from directly pressed seed kernel and aqueous extract of custard-apple seed at concentration of 1.0% and 2.0% were significantly causing the mortality of the insect. The effectiveness of the substances in killing Helopeltis were not significantly different with propoxur and sipermethrin insecticides. Neem oil obtained from steamed seed before being pressed and aqueous leaf extracts were also causing high mortality but still lower than two substances previously applied. Number of eggs produced by Helopeltis treated with the neem oil and seed extract of custard-apple were also significantly lower than control.

Acceleration of Paraquat Biodegradation by Isolated Soil Bacteria Erni Martani; Nur Arfa Yanti; Sebastian Margino; Sri Noegrohati
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 1 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10105

Herbicides residues were reported to have impact on the ecosystem. It was thought that acceleration of paraquat degradation would minimize these impacts. Paraquat was persistent in peat soil due to low pH. This study was done to investigate the role of bacterial isolates on the acceleration of paraquat degradation, especially in peat soil. The bacteria were isolated from several kinds of Indonesian soils using enrichment culture technique in a modified N-free medium. The medium was added with paraquat at gradually increase-concentration from 10, 20, and 40 ppm (w/w). Examinations in paraquat degradation were done in two levels. The first was in a synthetic medium (N-free medium); the second was in soil extract medium. Two kinds of peat were used to make the soil extract media, i.e. fibric and saphric peat soils. Several bacterial isolates were able to degrade paraquat in N-free medium. However, the degradation mode was different with those in peat soil extract media. None of them degraded paraquat in fibric and saphric soil extract media. It was suggested that the environmental limiting factors were responsible to the failure of paraquat degradation. Two selected isolates were able to degrade paraquat when the pH value of the extract medium was enhanced to around 5.5. Bacterial isolate of SM1, which was isolated from acid sulfate soil of Central Kalimantan, was the best isolate which was able to degrade paraquat in synthetic medium and peat soil extract media, especially in fibric extract medium. It degraded around 30%of paraquat within 15 day. Experiments are being done to enhance paraquat degradation by inoculation of mixed cultures of selected bacterial isolates.

Propagation and Purification of Baculovirus oryctes Huger Susamto Somowiyarjo; YB Sumardiyono; Sedyo Hartono; Triharso Triharso; Jun Kobayashi
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

An isolate of Baculovirus oryctes, a possible biological control agent for coconut beetle (Oryctes rhinoceros Huger) from East Java was propagated and purified. The virus could be transmitted by feeding the imago with 10% sucrose containing virus from homogenate of infected beetles. Effectivity of virus to 9 healthy females by sexual copulation. Virus be succesfully purified by a method of Payne.

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Y. Andi Trisyono

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anditrisyono@ugm.ac.id

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+62274-523926

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jpti.faperta@ugm.ac.id

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Jalan Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Y. Andi Trisyono

Contact Email anditrisyono@ugm.ac.id

Phone +62274-523926

Journal Mail Official jpti.faperta@ugm.ac.id

Editorial Address Jalan Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Y. Andi Trisyono

Contact Email
anditrisyono@ugm.ac.id

Phone
+62274-523926

Journal Mail Official
jpti.faperta@ugm.ac.id

Editorial Address
Jalan Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA