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INDONESIA
INDONESIAN JOURNAL OF PHARMACY
Published by Universitas Gadjah Mada
ISSN : 23389427     EISSN : 23389486     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.
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Articles 9 Documents
 1 
Search results for , issue "Vol 31 No 2, 2020" : 9 Documents clear
FTIR Method for Peptide Content Estimation and Degradation Kinetic Study of Canarium Nut Protein Rozana Oktaviary; Ilma Nugrahani; Slamet Ibrahim Surantaatmadja; Tutus Gusdinar Kartawinata; Cintya Nurul Apsari
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp78

Abstract

Quantitative analysis of bioactive peptide mostly conducted by measuring the activity. While the determination of peptide content in natural sources has been conducted using various instruments, vibrational spectroscopy remains underutilized. Here, we attempted new developed method in peptide quantification and degradation kinetic analysis using Fourier-Transform Infrared Spectroscopy. Bovine Serum Albumin was used as standard protein in method development and validation. Peptide content was estimated by converting peak area to concentration. The method was used to estimate peptide content in Canarium nut protein and its hydrolysates, which potentially hold biological activity. Kinetic study was conducted with microwave as an accelerator for hydrolysis, an apparatus rarely used in peptide study. Amide I band on wavenumber range of 1724.05-1619.91 cm-1 was selected for analysis, considering its selectivity and linearity. The method also met other validation requirement, including accuracy and precision. When applied in quantitative analysis, the method was able to calculate peptide content decrease in Canarium nut protein after hydrolysis using papain (38.24%), pepsin (33.67%) and alkaline reagent (28.53%). In kinetic study, microwave-assisted peptide degradation exhibited logarithmic profile with the equation of y=-0.148ln(x)+0.9591 and R² value of 0.963. Based on these results, FTIR is useful in estimating peptide content and in analyzing degradation kinetic profile. 
ANTI-STRESS ACTIVITY OF SOME PLANTS EXTRACTS OF THE NORTH CAUCASUS FLORA Dmitry Igorevich Pozdnyakov; Similla Adzhiahmetova; Nadezhda Chervonnaya; Andrey Mamleev; Eduard Oganesyan
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp131

Abstract

To date, stress is a common medical and socially significant disease that requires rational pharmacotherapeutic correction.The anti-stress properties of ethnolic and aqueous extracts obtained from leaves of Ribes nigrum L., inflorescences of Gaillardia pulchella Foug., stems of Lysimachia punctata L were studied in this work. Acute stress was modeled by immobilization of rats for 2 hours. The test-extracts were administered per os prophylactically in a dose of 1/20 of LD50 (2000 mg/kg). The following parameters were evaluated: organs mass coefficient (adrenal glands, thymus, spleen), the number of stomach erosion; the biochemical changes in the blood serum (adrenaline, cortisol, total protein and glucose concentration); the mitochondrial function parameters in brain and myocardium (evaluation of mitochondrial pore transitional permeability opening and mitochondrial membrane potential).  70% ethanol extract from Gaillardia pulchella Foug. inflorescences has the highest anti-stress activity, the course application of which contributed to the normalization of the weight index of organs, a decrease in glucose concentration by 64.5% (p <0.05), and cortisol by 73.7% (p <0.05) and adrenaline - 78.9% (p <0.05) in the blood, an increase in total protein level by 62.5% (p <0.05) and stabilization of mitochondrial function.  The study showed a high anti-stress activity of 70 % ethanol extract from Gaillardia pulchella Foug. inflorescences.
QuEChERS and C18 as solid phase extraction sorbent - ultra-high performance liquid chromatography -ultraviolet-visible method for determination of parabens in cosmetics products Ngoc-Van Thi Nguyen
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp69

Abstract

Concerns are growing about human exposure to endocrine-disrupting chemicals (EDCs), especially during the preadolescent development stage. Parabens are prevalent EDCs widely used as additives in cosmetics. So, the determination of parabens in such products is important. In this study, we developed a reliable and sensitive method to determine simultaneously nine common parabens (methylparaben, ethylparaben, phenylparaben, benzylparaben, penthylparaben, and two groups of isomeric compounds include propylparaben, isopropyl paraben, and butylparaben, isobutylparaben) in cosmetics products. The QuEChERS and solid-phase extraction techniques are used for extraction parabens from non-surfactant cosmetics (perfume, mouth wash solution) and surfactant cosmetics (shampoo, cream, gel), respectively and quantified by using ultra-performance liquid chromatography coupled with the ultraviolet-visible detector. All nine compounds showed good linearity with regression coefficients predominantly above 0.990. The LOD and LOQ of parabens were 0.07 µg/mL; 0.2 µg/mL, respectively. The recoveries ranged from 80 to 110% with the relative standard deviations below 8%. The developed method was successfully applied to determine parabens in various commercial cosmetic products from a local supermarket and the total parabens concentrations are in a wide-ranged from 2.0 to 1270 mg/kg.
In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line Sara E. Gomaa; Matthew Friedersdorf; Hesham Ali El Enshasy; MB Abou-Donia
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp108

Abstract

In this research, the biological activities of five plant extracts from family Apiaceae; Italian Parsley (Petroselinum neapolitanum), Fennel (Foeniculum vulgare), Celery (Apium graveolens), Cilantro (Coriandrum sativum) and Dill (Anethum graveolens), were studied. Antiproliferative effect of eleven ethanol crude extracts was tested in Human Cervical (Hela) cancer cells. Results clearly demonstrated that all plant extracts showed high significant difference when compared to the negative control (DMSO).Parsley leaves extract, cilantro leaves extract and cilantro stems extract showed no significant difference with the positive control (Actinomycin D). As for, fennel bulb extracts, fennel stalks extracts, celery stems gave better results than the positive control with no significant difference through the 24, 48 and 72 h treatment. There were no significant difference between Fennel extracts and the positive control, which showed high effect on the cancer cells survival. There were no significant difference between both extracts of Cilantro leaves and stems through each time but the best result was after 72 h of treatments. Regarding Dill leaves and stems, cell numbers recorded no significant difference between the both on time dependent manner. Further investigation for ethanolic extracts of parsley leaves, fennel bulb, fennel stalks, celery stems, cilantro leaves and cilantro stems which showed better results than using the commercial drug Actinomycin D (25ml/ml) for 24 h treatment or less depending on concentrations manner. Also, further investigation on different types of cancer cell lines to avoid the toxic effect of chemotherapy.
THE EFFECT OF GIVING DELTAMETHRIN AND CYPERMETHRIN INSECTICIDES ON THE LEVEL OF INTRATESTICULAR TESTOSTERONE IN MALE WISTAR STRAIN RATS Triutomo, Devyanto Hadi; Puspitasari, Ika; Susidarti, Ratna Asmah
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp116

Abstract

The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
Design and Synthesis of Novel Derivatives of 4-(6-(4-Substituted Phenyl)-7H-[1,2,4] Triazolo[3,4-b][1,3,4]Thiadiazin-3- yl) Phenol as a Potent Inhibitor of Tubulin with Antitumor Activity Kubba, Ammar A. Razzak Mahmood; Shihab, Wurood Ahmed; Al-Shawi, Nada Naji; Jabir, Majid Shamki
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp92

Abstract

A new series of 4-(6-(4-substituted phenyl)-7H[1,2,4] triazolo[3,4b][1,3,4]thiadiazin-3-yl) phenol was synthesized and explored for anti-tubulin activity  using in silico and in vitro assay models. The starting nucleus, ethyl-4-hydroxybenzoate was treated with hydrazine hydrated, and converted to 4-hydroxybenzohydrazide (1). Then, (1) was stirred with CS2 and KOH in dry EtOH  to afford 2-(4-. hydroxyl benzoyl)hydrazine-1-carbodithioate, as a potassium salt (2). Compound (2) was used directly and refluxed with hydrazine hydrate to yield a parent nucleus, 4-(4-amino-5-mercapto-4H-1,2,4-triazol-3-yl)phenol (3). The target compounds (4-7), were synthesized by refluxing compound (3) with various substituted phenacyl bromides  using  sodium acetate as  a abase, The new compounds (3-7) were characterized using different spectroscopic analysis  techniques including IR, and 1HNMR.  The anticipated  modes  of binding to α- tubulin were also investigated by means of a molecular docking experiment. The binding mode revealed good agreement with the in vitro work with binding energies of (-38.77 and  -37.50 kcal/ mol) for the most potent compounds 7 and 4, respectively. The effect of synthetic novel compounds on cancer cell lines proliferation was screened  by MTT assay. The ability of the synthetic compounds to induce apoptosis process was tested using acridine orange/ ethidium  bromide staining. The synthetic novel compounds exhibited potent inhibitory effects on the growth and viability of cancer cell lines SKOV-3 and AMJ-13 cells. They inhibited  the proliferation and growth  of cancer cell lines at low concentrations, with IC50 values ranging from10.44 to 19.67 µg/mL against SKOV-3 cells, and for AMJ-13 cells,  the IC50 values were11.35 to 20.52 µg/mL. The effect of  the synthetic compounds on  the cell growth, and proliferation of cancer cell lines was associated with increased apoptosis.Our results demonstrated that the target compounds inhibited cancer cell lines proliferation, with a mechanism of action parallel to that of other tubulin inhibitors
Application of Simplex Lattice Design on the Optimization of Andrographolide Self Nanoemulsifying Drug Delivery System (SNEDDS) Indrati, Oktavia; Martien, Ronny; Rohman, Abdul; Nugroho, Akhmad Kharis
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp124

Abstract

Background: Optimization of self-nanoemulsifying drug delivery system (SNEDDS) formulation is an important step to obtain optimal formulation with desired characteristics.Objective: This present study was aimed to utilize simplex lattice design in optimizing andrographolide SNEDDS.Method: Simplex lattice design was employed to optimize andrographolide SNEDDS in which component of SNEDDS was selected as the independent factor while the charactheristics of SNEDDS was used as the responses. Capryol-90, Kolliphor RH 40, and propylene glycol were selected as the oil, surfactant, and co-surfactant, respectively. Optimization of andrographolide SNEDDS formulation was based on their characteristics including emulsification time, droplet size, and drug content. The optimized SNEDDS formulation was evaluated for emulsification time, droplet size, drug content, and zeta potensial.Results: The emulsification time, droplet size, drug content, and zeta potensial of the optimized andrographolide SNEDDS was found to be 1.21±0.03 min, 44.02±0.67 nm, 6.69±0.08 mg/g, and -40.63±0.76 mV, respectively.Conclusion: This result suggested that simplex lattice design is a suitable for efficiently optimizing the formulation of andrographolide SNEDDS.
Effect of Growth Factor In Callus Induction and Bioactive Compounds In Seed Explant of Kaffir Lime (Citrus hystrix DC.) Tunjung, Woro Anindito Sri; Fatonah, Vita; Christy, Ghea Putri; Triono, Sugeng; Hidayati, Lisna
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp61

Abstract

Our previous study showed that kaffir lime leaf extracts may have anti-cancer properties. However, production of  bioactive compounds is affected by environmental factors. Here, we present a method to control environmental conditions using in vitro culture techniques such as callus induction. Calluses were induced from seed embryo explants of kaffir lime on MS medium with combinations of 2,4-D and BAP at concentrations 1:0.5; 1:1; and 2:1, respectively. Fourty and 60 days-old calluses were extracted using chloroform and ethyl acetate and analyzed by GC-MS. Results showed all combinations of 2,4-D and BAP were able to induce callogenesis from seed embryo explants of kaffir lime with no significant differences of callus initiation time, biomass, morphology and growth rates. However differences were detected in the bioactive compound profiles. In kaffir lime callus, both fatty acids and secondary metabolites were detected. Specifically, in 40 days-old calluses (exponential growth phase) we detected α-pinene and 1.8–cineole in plants treated with 2,4-D: BAP at concentration 1:0.5 and 2:1. In 60 days-old calluses (stationary phase) we detected a number of compounds in plants treated with 2,4-D:BAP at concentrations of 1:0.5 and 2:1, including caryophyllene, linoleoyl chloride, thiogeraniol, stigmasterol, clianosterol, citronellal, neo-isopulegol, citronellol, geraniol, eugenol, cyclopropane, pristane, elemol and farnesol
The Effect of Brazilin from Caesalpinia sappan on Cell Cycle and Modulation and Cell Senescence in T47D cells Riris Jenie; Sri Handayani; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp84

Abstract

Ethanolic extract and brazilein-containing fraction of Caesalpinia sappan L., has been reported to inhibit cell proliferation in T47D (ER+ PR+/- cell, Luminal A subtype model). The Luminal A subtype is the most common subtype of breast cancer in Indonesian women. In this study, we explored the activity of the reduced form of brazilein, i.e. brazilin, in T47D cells proliferation and the mechanism that involved. The cytotoxicity activity of brazilin was observed using MTT assay. While the cell cycle modulation analysis was done by using flowcytometry, and the senescence assay was observed using S-A-β-galactosidase assay. The results showed that brazilin inhibited cell growth in a dose-dependent manner with an IC50 value of 50μM (or 14.3μg/mL). That was higher than a brazilein-containing fraction, which was reported previously by our group to have an IC50 value of 68μg/mL against the same cell. Cell cycle analysis showed that cells treated with brazilin were accumulated at the G2/M phase in a dose-dependent manner. Furthermore, cells treated with a combination of brazilin and doxorubicin was accumulated at the G2/M phase and sub G1 phase. Cells accumulation at sub G1 phase indicates that the cells undergo apoptosis. Our data of S-A-β-galactosidase assay showed that cells treated with 1/4IC50, 1/2IC50, and IC50 brazilin had lower senescent cells compared to the untreated cells. The morphology of cells treated with IC50 (50μM) brazilin changed. The cells shape became rounded, cells were shrinkage and detached from the well plate, indicating that cells may undergo apoptosis. These results suggested that brazilin was cytotoxic towards T47D cells and its combination with dox potentially induced apoptosis and decreased cell senescence. The ability of brazilin to decrease cell senescence provides new insight of utilization of C. sappan or its constituents, particularly brazilin, as anti-ageing.

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