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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 15 Documents
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Search results for , issue "Vol. 7 No. 2 (2017)" : 15 Documents clear
Microhabitat Assessment of Mangrove Pit Viper Cryptelytrops purpureomaculatus in Restricted Mangrove Area, Dumai, Riau Province, Sumatera Nia Kurniawan; Bagus Priambodo; Fitra Arya Dwi Nugraha; Andri Maulidi; Agung Sih Kurnianto
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.01

Abstract

Bandar Bakau is a mangrove conservation area with approximately 20 ha that preserves many kinds of fauna, including Mangrove Pit Viper Cryptelytrops purpureomaculatus. However, this species and its habitat are still poorly studied in Indonesia, particularly Sumatera island, despite it has a wide distribution in Southeast Asian countries. The main purpose of this study is to evaluate the microhabitat of Mangrove Pit Viper C. purpureomaculatus that found at a restricted mangrove area in Dumai, Riau Province. Visual Encounter Survey (VES) and deep interview methods were used in this study. The survey found six individuals of C. purpureomaculatus that mostly basking on the Avicennia marina and artificial building. Although Bandar Bakau is a tourism object, but no snakebite cases were found.
Species and Abundance of Sea Urchins (Diadematidae) on Different Environmental Pressure Conditions Pratama Diffi Samuel; Dewa Gede Raka; Bagyo Yanuwiyadi
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.10

Abstract

Genetic diversity of sea urchin are very high; there are no two organisms of the same species that are exactly alike. Dense aggregation of sea urchin is responsible for the destruction of marine algae communities on coral reef ecosystem. The purpose of this study is to identify the species and abundance of sea urchins at different environmental conditions, to analyze the influence of water conditions on the abundance of sea urchins and to analyze the role of sea urchin as water quality and abiotic environmental conditions bio-indicators. Data collection procedures carried out in three stage that are: assessment of the status of coral reefs, sea urchins sampling and analysis of data. Data condition of coral reefs is drawn using methods kick frequency. Urchin abundance data retrieval is done by timed swims or snorkeling and species of sea urchins seen through genetic approach, by DNA sequencing. Identify the types of sea urchins in this study conducted with the gene fragment sequences Cytochrome Oxidase subunit I (COI). Stages isolation of DNA-based procedures uses Gsync DNA Extraction Kit from Geneaid. The species that identify in this study is Diadema setosum. Location with high environmental pressures has a low quality of coral reef ecosystems and has a great abundance of sea urchins. This proves that the water environmental conditions greatly affect the abundance/density of sea urchins. In locations with relatively low environmental stress or a sheltered environment the abundance of sea urchins is low.
Cysteine, Malondyaldehide (MDA) and Glutathione (GSH) Levels in Marasmic Type Malnutrition Dwi Indah Cahyani; Anik Puryatni; Nur Permatasari
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.11

Abstract

Micronutrien deficiency in severe malnutrition will reduce antioxidant capacity that needed for oxidative stress defense. Cysteine, a non-essential amino acid, is one of an important component for reduced glutha-tione (GSH). This study aims to prove the difference between the levels of cysteine, MDA and GSH levels in children with marasmic malnutrition and well-nourished children and prove whether there is a relationship between those parameters. Fiftysix patients participated in this study were grouped into two groups of sam-ples that were marasmic type malnutrition group (28 patients) and control groups that were well nourished group (28 patients). Examination begins with a complete laboratory screening, followed by examination of cysteine, MDA and GSH level. Of the 28 patients included in marasmic type malnutrition group consisting of 15 male patients (53.6%) and 13 female patients (46.4%), while the well-nourished group consisted of 13 male patients (46.4%) and 15 female patients (53.6%). The average age is 54.61±56.35 months in the group of ma-rasmic type malnutrition and 48.25±45.34 months in the well-nourished group. By using the Mann Whitney test, there were significant difference between the levels of cysteine and GSH in marasmic malnutrition and control group (p = 0.000 and p = 0.000 respectively). Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels in marasmic malnutrition children shows no significant correlation (R = -0.206, p = 0.294; R = -0.036, p = 0.856; R = 0.210, p = 0.284 respectively). In well-nourished group Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels also shows no significant correlation (R = -0.053, p=0.789; R = -0.146, p = 0.458; R = -0.079, p = 0.688 re-spectively). From this study it can be concluded that there are significant differences of the levels of cysteine and GSH between severe malnutrition groups compared with the control one. But this study shows no signif-icant correlation between the levels of cysteine and GSH levels in marasmic type malnutrition and well-nourished children.
Growth and Development of Apogamous Adiantum lunulatum Burm. f. Gametophyte from Dry and Humid Areas in Java Island Risti Novitasari; Tatik Chikmawati; Sulistijorini Sulistijorini
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.15

Abstract

Adiantum lunulatum Burm. f. has been widely used in aesthetic needs and medical fields. Detail information about the life cycle of A. lunulatum is still unknown, particularly about its gametophyte generation. Present work aimed to study and compare the growth and development of the gametophytes of A. lunulatum from dry and humid areas. Research method consists of two stages: (1) Spores were collected from two locations, Pasuruan and Bogor and (2) Spores were planted and observed the growth and development of gametophyte. The result showed that natural habitat of the plant in various dry and humid areas, affecting the rate of growth and development rates of A. lunulatum gametophyte. The gametophyte from the dry area showed faster rate of growth and development than that of humid areas. The spores collected from dry area need 9 weeks for germination, growth, and development while the spores of humid area took 22 weeks.
Cytotoxic of Ganoderma lucidum in Colon Cancer through Cyclooxygenase 2 (COX-2) as Its Molecular Target Agustina Setiawati
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.14

Abstract

Many studies were designed explore chemopreventive activity of natural products on colon cancer especially addressing COX-2 as molecular target. Another promising source of natural product that potentially exhibit anticancer activity on colon cancer is Ganoderma lucidum. This study assessed selectivity of cytotoxic effect of G. lucidum extract on WiDr to Vero cells and investigated molecular mechanism on COX-2. G. lucidum ex-tract was prepared by reflux extraction method; in vitro anticancer was assayed by MTT method on WiDr and Vero cell line. This study applied apoptosis induction assay to observe cell death mechanism using double staining method; further COX-2 expression was stained by immunocytochemistry method. G. lucidum extract has cytotoxic effect on WiDr cells with IC50 135 µg/mL. However, the cytotoxic effect had low selectivity to-wards Vero cells with Selectivity Index (SI) 3.66. The extract induced apoptosis and suppressed COX-2 ex-pression in WiDr cells. G. lucidum extract was potential to be developed as anticancer agent towards colon cancer.

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