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Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Kampus Bukit Jimbaran, Jimbaran, Bali, Indonesia
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Jurnal Kimia (Journal of Chemistry)
Published by Universitas Udayana
ISSN : 19079850     EISSN : 25992740     DOI : 10.24843/JCHEM
Core Subject : Science,
Chemistry
Jurnal Kimia (Journal of Chemistry) publishes papers on all aspects of fundamental and applied chemistry. The journal is naturally broad in scope, welcomes submissions from across a range of disciplines, and reports both theoretical and experimental studies.
Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol. 1, No. 1 Januari 2007" : 5 Documents clear
KARAKTERISTIK ARUS DAN POTENSIAL KATODIK PADA PERLINDUNGAN SISTEM ARUS TERPASANG TERHADAP STAINLESS STEEL TYPE 304 DI LINGKUNGAN AIR LAUT Yuli Panca Asmara
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Cathodic protection basically reduces the corrosion rate of a metallic structure by reducing its corrosionpotential, bringing the metal closer to an immune state. So, to optimallize the protection, not only potential but alsoenvironment and system should be considered in designing cathodic protection. The changes of current provided by adirect current (DC) source should be a counted during the protection.The objective of this laboratory was to studed the polarization behaviour concerning 304 stainless steelimmersed in the artificial sweater based on ASTM D 1141 using potentiostat. This task was performed by recordingdata points (potential across a constant resistor) within a determined potential. Then, the surface morphology ofspecimen caused by polarisation potential was investigated using an optical microscope and SEM analysis.The current derived from the polarisation diagram by the determination of several points protectionpotential ranging from -730mV to -1100 mV tend to decrease. The sharp decrease of current occured at protectionpotential of -1100 mV (from 580 mA/m2to 450 mA/m2) there was a slight decrease at protection potential of -730mV. The decrease occurred was from 190 mA/m2 to 150 mA/m2. Potential of protection cathodic also influenced the free corrosion potential. At the protection potential of -730 mV, free corrosion potential was -225 mV, and was-450 mV at the protection potential of -1100mV.By visual examination it can be deduced that the decrease of current density occurred was caused by thegrowth of salt calcareous deposits. By SEM investigation it was indicated that the deposit was made of calcium andmagnesium as a result of the increase of pH during protection. The more detail analisys showed that this depositcreated local pitting corrosion and decreased pitting potential by 200 mV.
PENENTUAN pH OPTIMUM ISOLASI KARAGINAN DARI RUMPUT LAUT JENIS Eucheuma cottonii I G. A. G. Bawa; A. A. Bawa Putra; Ida Ratu Laila
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

This research aimed to determine the optimum pH for the isolation carrageenan from Eucheuma cottoniiseaweed. The isolation was conducted at various pH : 7.5, 8.0, 8.5, and 9.0. A TLC using methanol : water (5:1) asmobile phase from standard carrageenan, gave result on Rf value 0.74 as equal to sample on treatment condition atpH 8.0 and 8.5. whereas, sample on treatment condition at pH 7.5 and 9.0 gave Rf value 0.72. In using ethanol :water (3:1) as mobile phase gave result on Rf value 0.75 from both standard and sample treated at 7.5, 8.0, and 8.5,while at pH 9.0 gave Rf value 0.74. Melting point test from standard gave range value 240-242, while for sample ontreatment condition at pH 7.5, 8.0, 8.5, and 9.0 gave range value each such 242-244, 241-243, 240-242, and 240-243.Compounds identification with solubility test method using various solvent medium obviously showed that both ofstandard and sample from various treatment condition were insoluble such as in aquades 200C, NaCl 25% 200C and800C, saccharosse 65% 200C and chloroform. Otherwise, they were soluble in aquades and saccharosse 65% withtemperature 800C. Infrared spectrum standard compound and sample from various treatment condition indicatedthat they were identical. Carrageenan isolation produce the largest number of rendemen at pH 8.5 with about34.65%, while the variant of carrageenan isolate was classified by kappa carrageenan.
ANALISIS VARIASI NUKLEOTIDA DAERAH D-LOOP DNA MITOKONDRIA PADA SATU INDIVIDU SUKU BALI NORMAL Ketut Ratnayani; I Nengah Wirajana; A. A. I. A. M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Human mitochondrial DNA (mtDNA) has higher polimorfism level than nucleous genom, especially in theD-Loop region, which is a non coding region and the most polymorfic region in the mitochondrial genom. Theanalysis of variation of nucleotide sequence of D-Loop region can be used to determine the individual or ethnicidentity and also maternal familiar relationship. The research aims to determine nucleotide variant on Balineseindividue, which can be used as data base in determination of mtDNA genetical profile of Balinese ethnic in a biggerscale.To achieve the aims of the research, way the nucleotide sequence of one normal Balinese individue usingthe epithelia cells in the saliva. The methods were :1) the isolation of sample mtDNA, 2) the amplification of the DLoopregion of mtDNA by PCR, 3) sequencing and analysis of nucleotides sequence.The 0,4 kb fragment of the D-loop region mtDNA of the sample were successfully amplified, andsequenced of 402 pb. The research found 6 new variants or morfe different from Cambridge or Anderson sequence :variant 16223C®T, 16249T®C, 16259C®T, 16278C®T, 16316A®G, 16375C®A. The research also found thedeletion of T nucleotide on position 16362.
ISOLASI SENYAWA SITOTOKSIK DARI DAUN ANDONG (Cordyline terminalis Kunth) N. W. Bogoriani; Sri Rahayu Santi; I. A. R. Astiti Asih
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and identification one of cytotoxic fraction have been conducted from the leaves of Andong(Cordyline terminalis Kunth). The fraction having LC50 values of 41,64 ppm contains saponin. Major isolate (7.5 mgwhite amorphous solid) was obtained after a series of chromatographic separations (gravity column chromatography,and preparative high performance liquid chromatography). Identification of the isolate using mass spectrometry withpositive electrospray showed MW of 866 as calculated from the ion peaks m/z 889[M + Na]+, and 867[M + H]+.The ion peaks at m/z 721[(M + H) - 146]+, 703[(M + H) - 164]+, 575[(M + H) - 292]+, 557[(M + H) - 310]+ , 429[(M+ H) - 438]+, and 411 [(M + H) - 456]+ of its fragments indicate the presence of three sugars (two terminal sugarsand one central sugar) from methylpentose moiety with MW 164 eash linked to an agyicone of MW of 428. Protonmagnetic resonance spectrum of the isolate in pyridine-d5 showed characteristic proton signals for three steroidmethyls (two angular methyls and one secondary methyl) at d 1.37 (s), 0.84 (s) and 1.02 (d, J = 6.6 Hz); anexomethylene group at d 4.79 ppm and 4.71 (each br s); an ethylene group at d 5.52 ppm (br d, J = 5.4 Hz); signalsof the protons linked to C26 at d 4.01 and 4.44 ppm (each d, J = 12.0 Hz), and three anomeric protons at d 6.43 ppm(br s); 5.56 ppm (br s) and 4.57 ppm (d, J = 7.0 Hz). From the above data it can be indicated that the isolate resultedis spirostan steroidal saponin.
KARAKTERISASI TOXOPLASMA GONDII ISOLAT INDONESIA Sagung Chandra Yowani; Endang Kumolosasi; Marlia Singgih Wibowo
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Cathodic protection basically reduces the corrosion rate of a metallic structure by reducing its corrosionpotential, Toxoplasma gondii isolated from diaphragm of sheep at an abbatoir in Sukabumi, West Java had beencharacterized by Centre Research Institute for Animal Sciences. The characterization included study of morphologyby optical microscope, study of ultrastructure by transmission electron microscope, the study of the parasite growthin mice Mus musculus, and study of proteins of the parasite. The growth of parasite in mice had been studied usingtwo groups of mice i.e., normal group and immunosuppressed group. The number of parasites was comparedstatistically using student’ t-pair test. Results showed that dexamethasone at a dose of 5.2 mg/20 g body weight intraperitoneally to the immunosuppresed mice did not increase the number of extracelluler parasites in the peritonealfluid. The best parasite harvest time was on the 4th day after inoculation. Determination of parasite protein obtainedat 4 days after inoculation using sodium dodecyl sulphate polyacrylamide gel electrophoresis showed a dominantsurface protein of 42 kDa.

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