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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

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Articles 7 Documents

Search results for , issue "Vol 6 No 1 (2018)" : 7 Documents clear

PCR FINGERPRINTING OF DIVERSE GENOMES FROM BACTERIAL STRAINS USING UNIVERSAL RICE PRIMER (URP) Hee Wan Kang
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Twenty primers of 20 mer referred to universal rice primer (URP) were developed from a repetitive sequence of rice genome. URP-PCR protocol employed stringent PCR with high annealing temperature throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, each single URP primer produced characteristic fingerprints from diverse genomes of bacterial species. The universal application of URP-PCR was demonstrated by applying it to 24 strains from Pectobacterium carotovoum subsp. carotovorum, 41 Agrobacterium vitis strains, 3 Xanthomonas spp. 5 Pseudomonas spp, Rhizobium sp. plant pathogenic bacteria, human and animal pathogenic bacterial strains including 6 Escherichia coli, 4 Salmonella spp., 7 Mycobacterium spp and 3 Blucella abortus strains. In addition, thermophilic bacteria were randomly isolated form high temperature compost and their URP-PCR polymorphisms were characterized with genetic relatedness. PCR approach using URP primers will be useful for studying DNA diversity of diverse prokaryotic genomes, especially at inter- and intra species levels.

ISOLATION, CHARACTERIZATION AND INOCULUM FORMULATION OF NODULE FORMING BACTERIA OF KUDZU (Pueraria phaseoloides (Roxb.)Benth.) FOR COASTAL SANDY LAND CONSERVATION Nike Triwahyuningsih; Tati Budi Kusmiyarti
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Kudzu plantation (Pueraria phaseoloides) as legume cover crop is one of alternatives in coastal sandy land conservation. The crops are known to associate with a root nodule-forming bacteria (Rhizobium sp.) which give some benefits to nutrient cycling i.e. : atmospheric N2 fixing and play role as soil conditioner; soil Nitrogen enrichment; nutrient cycling; and increasing other nutrients availability. A research to study the isolation dan bacterial inoculum multiplication from wild kudzu root nodules, compatible isolates screening and selected isolates multiplication, and examining the form, amounts and most proper inoculum application method was conducted in Greenhouse and Laboratory of Microbiology in Yogyakarta province.The research were held in four phases : (1) isolation, purification and characterization of isolates; (2) reinoculation dan compatibility testing of isolates to kudzu seeds; (3) inoculum multiplication; and (4) examination of the form, amounts and most proper inoculum application method. Physical and biochemical properties of the isolates were observed during the isolation phase. Infection and nodulation activity were observed during the reinoculation phase. Indirectly counting of the microbial numbers to obtain the cell numbers was conducted during the inoculum multiplication. While infection and nodulation activity and plant growth were observed during the inoculum testing phase.Isolates purification on Yeast Mannitol Agar + congo-red media gave 5 different isolates named RP-Etp1, RP-Etp2, RP-Etp3, RP-Etp4, RP-Etp5. The RP-Etp4 isolate had the highest compatibility to the kudzu seeds (number of effective nodules >100 per plant), followed by RP-Etp5 (medium compatibility, number of effective nodules 50–100 per plant), RP-Etp1 and RP-Etp3 (low compatibility, number of effective nodules < 10). Isolate RP-Etp2 was incompatible to the kudzu.Optimum cell numbers was reached in 48 hours incubation time. Application of broth/liquid inoculum of Rhizobium sp. has advantages over solid inoculum (in peat) as it gives the highest number of nodules, and the optimum dosage was 2 – 4 ml per plant. The highest infection-nodulation activity and plant growth were reached in 4 ml inoculum per plant (direct application) or 2 ml inoculum per plant (weekly applied in two weeks).

SCIENCE AND TECHNOLOGICAL ASPECTS IN RELIGIOUS RITUALS IN THE WOMEN FARMERS GROUP OF JEMPIRING KABUPATEN BADUNG, BALI, INDONESIA Ni Wayan Suryathi; Made Antara; Nengah Bawa Atmaja; Wayan Windia
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

This study aims to analyse the science and technological values in religious rituals in the women farmers group of Jempiring Kabupaten Badung. The data was analysed qualitatively. The results show: science and technological values in religious rituals which are implemented through Tri Hita Karana which are harmonious relationship with God, harmonious relationship with other people, and harmonious relationship with the environment, it is evident from Banten Pejati that is complete with segehan Agung which is given on piodalan at Pura Melanting. Ans it is also followed by pemerastitaan and pelukatan rituals for the group’s members. Daksina in Banten Pejati is the symbol of God’s palace. This symbol provides a scientific-psycho-theological effect that makes the minds of the people moored in the presence of a metaphysical god in the support of physical daksina. This value is also found in the sound of kul-kul, genta, gamelan, kidung and mantra during rituals. A harmonious relationship with other people is also seen from the behavior of good and honest members with a basis of menyame braya.

IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Ni Putu Adriani Astiti; Jeremy Pramana
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.

MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE I Putu Sudiarta; Dwi Martiningsia; I Nyoman Wijaya
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.

INCREASE VARIATION ON POTATO ‘GRANOLA’ USING GAMMA RAY IRRADIATION Muhammad Ibadullah; Ida Ayu Astarini; Eniek Kriswiyanti
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Potato is one of the main carbohydrate sources around the world, including Indonesia. Potato production in Bali generally does not use good quality of potato seed, causing disease infection and reduce productivity. An alternative effort to produce high quality potato is by induce mutation of tuber using gamma ray irradiation. This study aims to find out percentage of survival after irradiation of ‘Granola’ potato shoots and determine the post-irradiation potato growth and productivity. This research was conducted at Laboratory of Central Application of Isotope and Irradiation (PAIR), Pasar Jumat, Batan, Jakarta and UPT BBITPH Bedugul, Bali. Planting materials were early generation (G0) potato seed tubers. This study employ completely randomized factorial design with one factor, i.e. irradiation doses of 0, 20 gy and 40 Gy. Variable observed included percentage of shoots survive, and variations in production. Results showed that 20 Gy was the best dose to increase tuber production.

GASTROINTESTINAL PARASITE INFECTION ON BALI CATTLE RAISED IN SEMI-INTENSIVE FARMING SYSTEM IN DOMPU, SUMBAWA ISLAND: A PRELIMINARY STUDY Made Sriasih; Oscar Yanuarianto; Dahlanuddin Dahlanuddin; William Ernest Pomroy
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

To date, there are very few studies have been carried out on the effect of parasite infection on Bali cattle, and more importantly, there is no published data on the epidemiology of worm infections on Bali cattle in the island of Sumbawa, Indonesia. This research aimed to undertake a preliminary study on gastrointestinal (GI) parasitic infection on Bali cattle raised under semi-intensive farming system in five different sites in district of Dompu, Sumbawa island. Fecal samples of 76 Bali cattle, from three farmer groups namely site number 1, 4 and 5, were collected, processed and examined under a microscope, with 100x magnification, for egg fecal counting or oocyst fecal counting using the modified McMaster technique. The number of eggs/oocysts observed was then determined and identified. The results show that the eggs/oocysts ranged from 50-14800. The types of GI parasites identified were Strongyloides sp, Strongyle sp, Toxocara sp, Trichuris sp, and Coccidia. Coccidia oocyst is a type of parasite which was found with the highest percentage of 23.68% followed by Strongyle sp. (18.42%) and Toxocara sp. (10.52%). The overall prevalence of GI parasitic infection in cattle accounted for 43.42%. Young calves (0-3 months old) was more susceptible to GI parasitic infection among other age groups. This preliminary data could be used as a basic data on gastrointestinal parasitic infection in Dompu district. These results may also indicate the need to develop control strategies aiming at lowering the gastrointestinal parasitism.

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