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INDONESIA
Journal of Agricultural Science and Biotechnology
Published by Universitas Udayana
ISSN : 23020113     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara luas.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 7 No 1 (2018)" : 5 Documents clear
BAKTERI PELARUT FOSFAT MEMICU KETAHANAN TANAMAN KEDELAI (Glycine max) TERHADAP PENYAKIT SOYBEAN MOSAIC VIRUS Ni Nyoman Alit Purwaningsih; I Dewa Nyoman Nyana; I Gede Rai Maya Temaja; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Soybean (Glycine max) is an important commodity in Indonesia. The high utilization of soybean has not been accompanied by the sufficiency of amount of its production, even decreased from year to year. One of the factors that cause the decreased of soybean productivity is soybean mosaic disease caused by viral pathogens. Soybean mosaic virus is one of the most important types of virus in soybean could cause a decreased in yield until 25-90%.This study aimed to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to providing resistance to SMV disease on soybean plant. Six isolates rhizobacteria that could be isolated from Leguminosae plants had used as a treatment in the test. The result of field test showed three isolates namely R13, R12, and R9 able to increase soybean plant resistance, so each isolates made influence to crop yield. The results of identification based on the analysis of 16S rRNA gene, isolate R13 which gave the best response to soybean plant resistance and had percentage similarity between 94-98% with species Empedobacter brevis (Flavobacterium breve).
KARAKTERISTIK FENOTIF ISOLAT KLINIK Escherichia coli O157:H7 PADA MEDIA SORBITOL MAC CONKEY AGAR (SMAC) Wahyu Hidayati; I Gede Rai Maya Temaja; Ni Nengah Dwi Fatmawati
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Phenotypic Characteristic of Escherichia coli O157: H7 Clinical Isolates on MacConkey Agar with Sorbitol (SMAC)Escherichia coli is a Gram-negative, rod-shaped bacterium, and commonly has flagella. Most of E. coli strains are normal flora in digestive tract of human, but some serotypes are pathogen for human and animal. One of pathogenic E. coli strain that causes a severe infection in humans (hemorrhagic colitis and hemolytic uremic syndrome) is known as Enterohemorrhagic Escherichia coli (EHEC) with cattle as their natural reservoir. E. coli O157:H7 is the most important and pathogenic serotype of EHEC which responsible for outbreak of Hemorrhagic Colitis and Hemolytic Uremia Syndrome. Accurate and cheap method detection of E. coli O157:H7 is needed to help early detection diagnosis and therapy. Therefore, the aim of this study is investigate phenotypic characteristics of E. coli O157:H7 isolated from clinical specimens using MacConkey Agar with Sorbitol (SMAC) media. Three E. coli clinical isolates from Sanglah General Hospital showed colorless non-sorbitol fermenting colony in SMAC media as phenotypic characteristic of E. coli O17:H7, therefore SMAC may beused on are of confirmation methods for E. coli O157:H7 detection from clinical isolats.
BIOEKOLOGI Grapevine leafroll-Associated Virus type 3 (GLRAV-3) PENYEBAB PENYAKIT DAUN MENGGULUNG PADA TANAMAN ANGGUR (Vitis vinifera L.) Yuyun Fitriani; I Gede Rai Maya Temaja; G. N. Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The survey of vineyard fields in Buleleng, Bali, Indonesia was conducted, some grapevine cultivars were found had grapevine leaf roll disease symptomps. The symptoms including interveinal reddening or yelowing and fall leaves, down rolling of leaves, phloem disruption, and the fruit maturation is delayed. The analysis of enzyme linked immunosorbent assay (ELISA) of symptom leaf with Grapevine leaf roll-associated virus type 3 (GLRaV-3) antiserum shown the disease was corresponding to the GLRaV-3. The study was conducted to identify the mealybug that colonized of grapevine, to determine capability of mealybug to transmitted GLRaV-3 and host range GLRaV-3.Base on the survey ofmealybuginvineyard fields in Buleleng, Bali, the two types of mealybug were identified, namely Ferrisia virgata (Ckll.) (Hemiptera: Pseudococcidae) and Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae). Results of the transmission test showed that only M. Hirsutus are capable of transmitting GLRaV-3. The plant range host test of GLRaV-3 inoculation with M. hirsutuswas conducted and the symptom was detected on3plants species, grapevines (Vitis vinifera), native gooseberry (Physalis floridana) andshoe flower (Hibiscus rosa-sinensis) from18 species of plants that used.
PEMANFAATAN BAKTERI PELARUT FOSFAT PENGINDUKSI HORMON IAA (Indol Acetic Acid) UNTUK PENINGKATAN PERTUMBUHAN KEDELAI ( Glycine max) Desak Putu Sudarmini; I Made Sudana; I Putu Sudiarta; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Soybean is one of the agricultural commodities that are needed to fulfil food nutrients of Indonesian people. The low production of this commodity in Indonesia has not yet been able to meet the high demand for soybean that reaches 2.8 million tons per year, so that the Indonesian government still import soybeans at the amount of 2.26 million tons annually. The low production of soybeans in Indonesia is due to several factors, one of which is the expensive price of chemical fertilizers The use of rhizobacteria as bio-controlling agents can increase the availability of nutrients as well as crop production. Phosphate solubilizing bacteria are rhizobacteria that have the capacity to solubilize bound phosphate as well as trigger the growth hormone IAA that is the main hormone that controls various physiological processes in plants. The current study aimed to investigate whether isolates derived from rhizospheres of Leguminosae plants can induce IAA hormone and foster the vegetative growth of soybeans.The research results showed that five out of nine isolates of the phosphate solubilizing bacteria are able to induce IAA hormone and foster the vegetative growth of soybeans. Isolate treatment that showed the best impact toward soybean growth was identified molecularly through PCR. The result of RHC6 isolate identification shows a DNA ladder size of 1300bp which is suitable with the primer used. Based on the phylogenetic analysis conducted, nucleotide sequence of RHC6 isolate from Bali has a high similarity (97%) to and a low genetic distance (0.018) from Brevundimonas diminuta isolate from the US.
PEMANFAATAN RHIZOBAKTERI PELARUT FOSFAT DARI TANAMAN LEGUM UNTUK PENINGKATAN PERTUMBUHAN DAN HASIL TANAMAN KEDELAI Ni Komang Budiyani; G. N. Alit Susanta Wirya; I Made Sudana; I Gusti Ngurah Raka
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.84 KB)

Abstract

Soybean is one of the strategic commodities in Indonesia. Soybean became the third most important crop after rice and maize. Leguminose plants are known to have microbial diversity in soils through their root exudates and can have a positive impact on plant growth. The use of rhizobacteria, known as Plant Growth Promoting Rhizobacteria (PGPR), is a bacteria that lives in the root area (rhizospher) and plays an important role in the growth of plants with the ability to form colonies around the roots quickly and can maintain environmental sustainability, one of bacteria rootsis phosphate solubilizing bacteria. This research is conducted to determine the influence of phosphate solubilizing bacteria on the growth and yield of soybean crops. This study used a factorial pattern (RAK) with 3 repetition and 9 treatments. Treatments of Rhizo 09 isolates from undis Rhizosfer here showed the best results.The analysis of sequencing and pyhlogeni shows that Rhizo 09 has close proximity withFlavobakterium johnsoniae from China and having a high similarity (98%) and a small genetic distance (0.003).

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