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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 14 Documents
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Search results for , issue " Vol 12, No 2 (2013)" : 14 Documents clear
ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers] Poerba, Yuyu Suryasari; Ahmad, Fajarudin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.81 KB) | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
MORTALITAS DAN PROFIL HEMATOLOGI MENCIT YANG DIINFEKSI Trypanosoma evansi ISOLAT BANGKALAN, PEMALANG DAN PIDIE [Mortality and Haematology Profiles of Mice Infected with Trypanosoma evansi from Bangkalan, Pemalang and Pidie Isolates] Subekti, Didik T; Febria, Mutiara; Sari, Febiola Rama; Hartiyati, Indri N
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.531

Abstract

Trypanosoma evansi known as trypanosomiasis causes or called surra in animals. Trypanosomiasis-associated death were generally due to severe anemia. Another report mentioned that a rapid death of mice can be attributed to the several reasons. One of those reasons is highly parasitaemia that leads to death of the host. However, there is another distinct report that high parasitemia in mice does not necessarily lead to anemia and clinical symptoms. This research was focused to study the relationship between trypanosomiasis-associated death with haematology profile, particularly the decrease of PCV (packed cell volume) value, anemia and virulence of T. evansi collected from several region of Indonesia. The experiments were performed by dividing mice into four groups. Three groups were infected by T.evansi according to the original isolates while another group were uninfected mice. Every two-day interval, all mice were observed their mortality and parasitaemia and also examined the PCV value and erythrocyte counts in peripheral blood. The experimental results shown that the degree of parasitemia in mice were not always related to mice mortality and anemia. Decreases of PCV value were related to the existence of parasitaemia but not with anemia. This study suggests that there is no relationship between the decreases of PCV value and anemia to the trypanosomiasis-associated death in mice.
PERAN GAMBUT TERHADAP NITROGEN TOTAL TANAH DI LAHAN RAWA [The Role of Peat on Total Nitrogen in The Wetland Soils] Fahmi, Arifin; Radjagukguk, Bostang
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1236.937 KB) | DOI: 10.14203/beritabiologi.v12i2.536

Abstract

Peatland has important role in wetland ecosystem stability. Depletion and disappearance of peat layer lead to loss of potential source of nutrient and disruption of wetland ecosystem stability. The research aimed to study the influence of peat thickness and land hydrological condition on the total nitrogen (N) content in soil, the influence of rapidly or naturally the depletion and disappear of peat layer on total N content in soil, and the influence of hydrological condition on total N content in soil. Total N was observed in June of 2009 and 2010 (transition from wet to dry season), September of 2009 and 2010 (peak of dry season), and January of 2010 and 2011 (peak of wet season). The research was carried out on potential acid sulphate soil (A), peaty acid sulphate soil (B),shallow peat which all of peat layers wereremoved (C), shallow peat which peat layer was partially removed (D), shallow peat (E), moderate peat (F) and deep peat (G). The results showed that total N content increased with increasing of peat thickness, the depletion and disappearance of peat layer reduced total N content in soil, and declining in the groundwater level increased total N content in soil.
EVALUASI KUALITAS BEBERAPA GENOTIPE BAYAM (Amaranthus sp) PADA PENANAMAN DI JAWA BARAT [Quality Evaluation of Some Genotype of Spinach (Amaranthus sp.) Cultivated in West Java] Rahayu, Suwarni Tri; Asgar, Ali; Hidayat, Iteu M; Kusmana, Kusmana; Djuariah, Diny
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.527

Abstract

Spinach is one of leafy vegetables species that contain lots of vitamins and minerals. This study aims to examine the quality of several genotypes of spinach grown at two highland sites of West Java (Cipanas and Lembang).This experiment was conducted using Split Plot design,with five lines and five replications. Three genotype were tested, namely B1 = By16 , B2 = By18 , and B3 = By21, as well as two commercial varieties on the market spinach (B4 = red Giti , B5 = Kusuma).Measurements included physical parameters (length and width of leaves,stem diameter, texture, and color) and chemical parameters (water content, vitamin C, and fiber). The experiment was conducted in the laboratory of Physiology Vegetable Research Institute, Lembang from March to September 2012.The results showed that the content of vitamin C and fiber in genotype B1 and B2 were not significantly different from the control genotype.Similarly, the results of measurements of the length and width parameters of the leaves on all the tested genotype was not significantly different from the control genotype. Average length and width leaves of the genotype tested in Cipanas significantly higher than those tested in Lembang. Spinach has a shelf life of 1 day at room temperature in the storage area of Lembang ( 21±2°C and 85% RH ).

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