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BERITA BIOLOGI
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Articles 30 Documents
Search results for , issue "Vol 10, No 6 (2011)" : 30 Documents clear
KAJIAN OZONISASI (O3) TERHADAP KARAKTERISTIK KUBIS BUNGA {Brassica oleracea var. botrytis) SEGAR SELAMA PENYIMPANAN PADA SUHU DINGIN Asgar, Ali; Sugiarto, A T; Sumartini, Sumartini; Ariani, D
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.997 KB) | DOI: 10.14203/beritabiologi.v10i6.1947

Abstract

The aims and objectives of this study are to determine the effect of ozonization on the characteristics of cauliflower (Brassica oleraceae var.botrytis) during cold storage.The benefit of this research is to prolong the storage period of cabbage flowers with characteristic and quality be appropriate to consumers. The design used is the Randomized Block Design with 6 replications. The treatments consisted of 0 ppm ozone concentration bear leaves, 1.5 ppm ozone concentration without leaves, 1.5 ppm ozone concentration bearing leaves, and 1.5 ppm ozone concentrations without leaves.The parameters observed were weight loss, hardness, moisture content, vitamin C, heavy metals Hg and Ag,number of microorganisms, organoleptic color, freshness and appearance. The results showed that the best treatment was 1.5 ppm ozoneconcentrations with cabbage flowers and leaves. This was caused by ozone acts in sterilization (ozone can kill a variety of pathogenic microorganisms such as bacteria, viruses and fungi), deodorant (ozone eliminating odors caused by a variety of organic compounds and microorganisms), decoloration (ozone can eliminate organic color), and degradation (ozone can break down various organic compounds and heavy metals).
PENGARUH PEMUPUKAN BERIMBANG TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KENTANG (Solatium tuberosum L.) VARIETAS GRANOLA Edi, Syafri; Endrizal, Endrizal
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1935

Abstract

Potato is one of the main vegetables in Kerinci District. Development of potato plants is supported by the availability of land, agro ecosystem, labor and market opportunities as well as conductive local government policies. Potato farmers do not fully implementing the recommended farming technologies, such as the use of good seed, balanced fertilizer, crop rotation, pest control, harvesting and post harvest, so the production and reception of farmers is still low. The research took place in Mount Seven Village, Pelompek Kerinci District, Jambi Province which is background by agro-ecosystem dryland upland wet climate (LKDTIB), with soil type andisol at an altitude of 1400 m above sea level. The study aimed to see the effect of balanced fertilization on the growth and yield of potato. Potato used is Granola variety fourth generation derived from the Parent Seed Potato Institute in Kayu Aro. Assessments carried out from February until July 2008, with the treatment as follows: Introduction Package (A): SP-36 450 kg, 200 kg Urea, ZA 200 kg, 300 kg of KC1, NPK 100 kg and 10,000 kg of organic fertilizer/ha. Farmers Package (B): SP-36 750 kg, 400 kg Urea, 250 kg of KC1, and 400 kg NPK /ha. Fanners Package (C): SP-36 600 kg, 300 kg ZA, KC1 300 and 400 kg NPK /ha. The results showed that Package (A) gives the results of 24,320 kg /ha, higher than package (B) with the results of 18,240 kg/ha, while the Fanners Package (C) get the 19,520 kg/ha. High yield Package (A) is supported by the controlled growth of plants, the intensity and the population of pests and diseases and relatively more yield components than Package(B) and Package (C).
ANALISIS TINGKAT PENCEMARAN AIR DENGAN METODE INDEKS PENCEMARAN DI TELUK YOUTEFA, JAYAPURA, PROVINSI PAPUA Manalu, Janviter; Nurjaya, I Wayan; HS, Surjono; Kholil, Kholil
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1942

Abstract

Youtefa Bay is one of the potential natural recources, is located on the western side of Jayapura City,Papua province.The waters has beenutilized for fishery, transportation and ecotourisme.Youtefa Bay surroundings are settlements where the most of residents are fishermen and farmers.The aim of this study is to determine the pollution level of the Youtefa Bay,approached by pollution index method.The results showed that the water condition of Youtefa Bay was slightly to moderately polluted.Due to pollution, it is therefore very importance to periodically monitor the water quality of the Youtefa Bay to maintain its sustainability.
KEEFEKTIFAN BAHAN PELINDUNG ALAMI DALAM MEMPERTAHANKAN INFEKTIVITAS Spodoptera exigua NUCLEOPOLYHEDROVIRUS (SeNPV) Samsudin, Samsudin; Santoso, Teguh; Rauf, Aunu; Kusumah, Yayi Munara
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.322 KB) | DOI: 10.14203/beritabiologi.v10i6.1932

Abstract

Spodoptera exigua nucleopolyhedrovirus (SeNPV) is a viral pathogen of onion caterpillar S. exigua with high pathogenicity. One of the major constraints to the use of SeNPV for biocontrol of onion caterpillar is its sensitivity to ultraviolet (UV) degradation. The purposes of this research were to determine the effect of sunlight exposure on the virulence of SeNPV and to find out the effective natural UV protectant to maintain the SeNPV virulence. The results showed that the sunlight radiation affects the SeNPV infectivity. Addition of 1% of coconut shell charcoal, lampblack, husk charcoal, yam flour, molasses, yam filtrate, turmeric filtrate and green tea filtrate to the SeNPV suspension were found to be effective as UV protectant. Coconut shell charcoal, lampblack and husk charcoal are activated carbon that can absorb UV light. Yam filtrate is a natural ingredient that contains saponins and is able to protect SeNPV particles as reflectance. While molasses, turmeric filtrate and green tea filtrate containing flavanoid serve as a protective virus particles and UV absorber.
HETEROSIS, HETEROBELTIOSIS DAN TINDAK GEN KARAKTER AGRONOMIK KEDELAI {Glycine max (L.) Merrill} Krisnawati, Ayda; Adie, MM
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1022.153 KB) | DOI: 10.14203/beritabiologi.v10i6.1952

Abstract

Most of the soybean {Gfycine max (L.) Merrill (varieties in Indonesia are produced through hybridization. Expected value of heterobeltiosis,heterosis and the gene action is needed to know to optimize the development strategy of soybean varieties. The experiment was conducted in The Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian (Balitkabi) greenhouse. The first stage was to form Fl hybrid seed,which was done from May to July 2009; the second stage was for the assessment of Fl plants (August-November 2009). Heterosis,heterobeltiosis and the potential ratio are calculated from the 18 crosses combinations derived from 6 parental genotypes. Data used include the flowering day (days), maturity day (days), plant height (cm), number of branches/plant, number of node/plant, number of pods/plant,seed yield/plant (g) and SO seeds weight (g). Cross combinations that have the highest heterosis values are on the flowering day, maturity day, number of branches/plant and number of node/plant; heterobeltiosis also showed the highest value on the same agronomic characters.Heterosis value of the character of seeds per plant ranged from -36.50% to 75.49%, while for heterobeltiosis ranged from -48.68% to 58.31%. The highest heterosis value was indicated by the Fl from crosses Willis x Malabar. The highest heterobeltiosis value was indicated by crosses of Willis x Grobogan, which means that these crosses have the 58.31% higher yield than the best parents. The soybean yield is controlled by dominant genes and over-dominant. Combination crosses Willis X MLG 0706 (reciprocal) has a higher potential ratio for die character of the number of pods and seeds yield/plant compared to the value of the other potence ratio. Development of soybeans with the use of hybrid heterosis phenomenon still needs to be assessed in terms of economic feasibility.
SIFAT PROTEKSI EKSTRAK AIR PANAS TEH {Camellia sinensis (L.) Kuntze} HIJAU PADA KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARACETAMOL Heddy Julistiono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1943

Abstract

In order to develop yeast Candida tropicalis as a model cell for evaluation of substance having anti- or pro-oxidant activity in cell level, the effect of hot water tea {Camellia sinensis (LJ Kuntze) extract on peroxidized lipids, a marker of oxidative stress and cell mortality in the yeast caused by paracetamol has been evaluated. Incubation of yeast cell in the presence of 1.38 % green tea for 2 h decreased cell viability followed with increasing of peroxidized lipids, whereas 0.27 % green tea caused increasing of peroxidized lipids without causing cell death.Yeast cell was not affected by 0.1 % green tea. Incubation of yeast cell with presence of 0.15 % (g/v) paracetamol for 2 h did not cause cell mortality, however content of peroxidized lipids increased significantly. In the presence of higher dose of paracetamol (0.3 %) cell viability remarkably decreased and followed with increasing of peroxidized lipids significantly. Green tea of 0.1 % increased cell viability of cells treated with 0.3 % paracetamol while peroxidised lipids decreased. The data indicated that high dose of paracetamol caused oxidative stress in yeast cells, while green tea with lower concentration might protect paracetamol toxicity due to its antioxidant property. Although the antioxidant property, high dose of green tea could cause oxidation due to its pro-oxidant activity. In conclusion, yeast C. tropicalis could be potentially used as a model cell to evaluate substances having antioxidant property in cell level.
IDENTIFIKASI Lernaea sp. YANG MENGINFEKSI IKAN ARWANA IRIAN {{Scleropagesjardinii (Saville-Kent, 1892)} DI MERAUKE, JAKARTA, BOGOR DAN DEPOK Dikry N Shatrie; Kurniasih Imamudin; Wisnu Nurcahyo; Triyanto Triyanto
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1950

Abstract

Lemaea spp. is very important fish ectoparasites because it spreads all over the world and its economic importance has increased due to numerous epizootics occurrence among the most important farmed fish; and has wide host range. Lemaea sp. has been infected not only the family Cyprinidae and Characidae but also in Osteoglossidae family, such as Red Arwana [Scleropagesformosus) in Malaysia and Arwana fish {Scleropagesjardinii (Saville-Kent, 1892)) in Irian.The objective of this study was to identity Lernaea sp. often attacked Arwana irian fish as a precautionary measure the spread of infection, especially on Arwana irian fish. Arwana irian fish were collected from Merauke, Jakarta, Bogor and Depok, 10 infected fish per area. Found Lernaea sp. fixed in glutaraldehyde for morphology study. Based on morphological charactheristic, there were six different Lemaea sp. attacked Arwana irian fish. They are Lemaea cyprinacea, L. devastatrix, L. lophiara, L.oryzophila, L. papuensis and Lemaea n sp. Lemaea n sp. was considered as a new species due to different variation of holdfast organ.
HIDROLISIS KITIN MELALUI FERMENTASI SEMI PADAT UNTUK PRODUKSI N-ASETILGLUKOSAMINA Iwan Saskiawan; Rini Handayani
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1937

Abstract

N-acetyl-D-glucosamine (GlcNAc), the monomeric unit of polymer chitin has attracted much attention for their therapeutic activity in osteoarthritis.It is mainly produced by acid hydrolysis of chitin which affect the environmental problem because of its acidic wastes.Therefore, it is need to develop the new method for GlcNAc production. The aim of this experiment is to produce GlcNAc by mean of submerged fermentation of chitin. The preliminary study showed that fermentation of chitin by Aspergillus sp.501 produced higher GlcNAc than that of Saccharomyces sp. It was 1.39 ng/ml and 1.07 ng/ml. Then the Aspergillus sp. 501 was used in optimation of GlcNAc production. The effect of pH and nitrogen course such as bacto peptone, yeast extract, amonium sulfat and urea to GlcNAc production was examined. Then the product of GlcNAc was precipited by vacum evapotated and freeze dryed. The results showed that the highest of production GlcNAc of 2.228 ng/mL was obtained on pH 4 of medium solid state fermentation using urea as Nitrogen source at 10 days incubation.
INFEKSI Salmonella enteritidis PADA TELUR AYAM DAN MANUSIA SERTA RESISTENSINYA TERHADAP ANTIMIKROBA Anni Kusumaningsih; M Sudarwanto
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1944

Abstract

Salmonella enteritidis is one of pathogenic bacteria in chicken and human with re-emerging foodbome pathogen. Uncontrol use of antimicrobial drugs for the prevention and treatment of infectious diseases in chicken and human may lead to the development of antimicrobial resistance. Sampels of chicken eggs were collected from markets, layer farms, and grant parent stock farms. Sampels from human were anal swabs collected from hospitals and a laboratory of microbiology. Identification of bacteria was conducted by isolation and biotyping using selected media for Salmonella, while serotyping S. enteritidis was conducted with spesific 0 somatic (1, 9, and 12) and H flageIJa (m) antigen.Antimicrobial resistance tests were conducted by the standard diffusion method using antimicrobial disks.The results of isolation and identification of Salmonella show that 9 out of 122 (7.4%) sampels of layer eggs and 7 out of 23 (30.4%) sampels of embrioneted eggs were positive with Salmonella spp. The results of serotyping against S. enteritidis show that 7 out of 9 (77.0%) from layer eggs and 7 out of 7 (100.0%) from embrioneted eggs were positive with S. enteritidis.From the IS anal swabs of human were obtained 14 (93,3%) contain S. enteritidis.The antimicrobial resistance profiles of S. enteritidis isolated from layer eggs show high resistances to streptomycin (42.9%), neomycin (85.7%), doxicycline (64.3%), and ciprofloxacin (57,1%), whereas those S. enteritidis isolated from human show high resistances to streptomycin (50.0%), neomicyn (85.7%), tetracycline (42.9%), and doxicycline (42.9%).Multiple resistance profiles of S. enteritidis isolated from eggs were mostly to 2-3 antibiotics and those of S. enteritidis isolated from human were mostly to more than 5 antibiotics.
KERAGAMAN GENETIK HlBRIDA BEBERAPA STRAIN IKAN NILA {Oreochromis niloticus Bleeker) Rudhy Gustiano; Dinar Soelistyowati; Agung Luthfi Fauzan; Otong Zenal Arifin
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1951

Abstract

Objectives of this study are to determine the genetic variability among progeny of intraspecific crossing between three strains of Oreochromis niloticus Bleeker (BEST, Nirwana, and Red NIFI) using Randomly Amplified Polymorphic DNA (RAPD) and truss morphometric. The results showed that hybridization enabled to increase genetic variability respectedly on progeny of Nirwana male crossed to BEST female (27,93%) as well as to Red NIFI female. An analysist of truss morphometric indicated that BEST population had the lowest similarity and largest genetic distance (0,6) to the Nirwana and Red NIFI which were closer to the hybrid populations.

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