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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 30 Documents

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Search results for , issue "Vol 11, No 1 (2012)" : 30 Documents clear

SPATIAL MODEL OF SUMATRAN TIGER (Panthera tigris sumatrae) POTENTIAL HABITAT SUITABILITY IN BUKIT BARISAN SELATAN [Model Spasial Kesesuaian Habitat Harimau Sumatra (Panthera tigris sumatrae) di Taman Nasional Bukit Barisan Selatan, Indonesia] Suyadi, Suyadi; Jaya, I Nengah Surati; Wijanarto, Antonius B; Wibisono, Haryo Tabah
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This study describes on development of habitat suitability for Sumatran tiger in Bukit Barisan Selatan National Park (BBSNP). In this study remotely-sensed data set were linked with tiger and it prey survey using camera trap to identify the environmental and human factors that influences the tigers potential habitat, and to develop a spatial model as well as in BBSNP. All at once, the study showed that the potential model for estimating the tigers potential habitat suitability could be developed using environmental and human factors. The most significant factors that influence the tiger habitat suitability in Bukit Barisan Selatan National Park are human factors such as distance to road and distance to forest edge and the environmental factor i.e. the number of tiger prey. The verification of the model shows that the model is capable to estimate the probability of the tiger presence having accuracy of approximately 78%. The model shows that there is no significant difference between the predicted data and actual data and having mean deviation less than 10%.

IDENTIFIKASI ALEL GEN Xa7 PADA PLASMA NUTFAH PADI LOKAL PAREKALIGOLARA MELALUI UJI SEGREGASI FENOTIPE DAN GENOTIPE [Identification of Xa7 Alleles Gene in Landrace Parekaligolara by Phenotype and Genotype Segregation Analysis] Utami, Dwinita W; Kadir, TS; Nasution, A
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bacterial Leaves Blight (BLB) is one of the major diseases in rice. Among the disease resistance genes HDB, Xa7 gene still can compensate the diversity of Indonesian BLB pathogen in the field. One of the local rice germplasm, Parekaligolara (Indica, IRN: 1541) were selected based on allele mining research which contained a Xa7 alleles. The purpose of this study is to identify the alleles of the Xa7 gene contained in Indonesian local rice Parekaligolara germplasm in rice through the segregation testing based on phenotype and genotype performance of segregating population derived from the crossed with the susceptible control varieties, TN1. The segregation testing were conducted in F1 and F2 populations and analyzed on phenotypes of BLB resistance and genotypes using Xa7SNP markers. The analysis results showed that the Xa7 allele variation founded in the Parekaligolara germplasm are act as dominant alleles that contribute on the BLB resistance trait particularly to Race VIII of BLB pathogen. Xa7SNP8 is polymorphic markers for Xa7 alleles gene that are found in Parekaligolara. This marker could be used as a marker assisted for selection, if Parekaligolaras Xa7 alleles will use as a parent in the BLB disease resistant rice breeding program.

ANALISIS PERUBAHAN POLA GENETIK EMPAT GENERASI MANGGIS (Garcinia mangostana L.) BERDASARKAN MARKA ISSR [Analysis of Genetic Pattern Changes among Four Generations of Mangosteen (Garcinia mangostana L.) Based on ISSR Marker] Noorrohmah, Siti; Sobir, Sobir; Efendi, D.
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mangosteen reproduces trough apomixes mechanism, from which the seed develops without fertilization. Mangosteen is an obligate apomicts and it is believed that all of its progenies may have the same genotype as their mother plant. However it was found that genetic variations occured among mangosteen accessions. This research is aimed to study of genetic changing among generations mangosteen. The plant materials used were four generations (P1, P2, P3, and P4) of mangosteen taken from Wanayasa, Purwakarta.The samples were selected according to tree height and taken from each sector (north, east, south, and west). Genetic observations were conducted using Inter Simple Sequence Repeat (ISSR) technique. Ten primers were used to amplify the genomic DNA of mangosteen. It was found that the genetics pattern differed among tree of different age. More variation was observed on the older mangosteen tree.This information may be useful to determine the optimum age of mangosteen for becoming mother plant.

ANALISA VEGETASI TEMPAT TUMBUH Hoya purpureofusca Hook.f. DI RESORT SELABINTANA, TAMAN NASIONAL GUNUNG GEDE-PANGRANGO [Vegetation analysis of habitat Hoya purpureofusca Hook.f. at the Selabintana Resort, Mount Gede-Pangrango National Park] Hidayat, Syamsul; Rahayu, Sri; Ningtyas, Kartika
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Research on vegetation, where Hoya purpureofusca Hook.f. grows, has done in the Selabintana Resort, Mount Gede-Pangrango National Park in September 2011. Purposive random sampling was set up for a total of 18 square plots of 10m x 10m, where placed on two different sites. Nine plots were placed in the growing sites of H. purpureofusca, otherwise were placed in other sites. Observation was made in each sampling plots on the number of species at three stages, i.e. seedling, sapling and tree in each sampling plots. The diversity indices (including species richness, heterogeneity, and evenness) were analyzed using Jakcknife index, Margalef index, Menhinick index, Simpson index and Shannon-Wiener index. Species dominance was analyzed by important value index and similarity index whereas species associations was analyzed by chi-square. Based on the analyses, there were differences on vegetation composition and diversity between growing site of H. purpureofusca and non growing site. Schima wallichii (DC.) Korth. is the dominant species that has an important role for the growth of H. purpureofusca.

KEANEKARAGAMAN JENIS TUMBUHAN PEMAKAN SERANGGA DAN LAJU FOTOSINTESISNYA DI PULAU NATUNA [Diversity of Insectivorous Plants and Its Photosynthetic Rate in Natuna Island] Mansur, Muhammad
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Studies of insectivorous plants diversity and photosynthesis rate of pitcher plants in Natuna Island was conducted on August 2011, Survey in eleven villages conducted to determine the species found in the visited sites, Bioscientific Portable LCI ADC Photosynthesis System used for measurement of pitcher plants photosynthesic rate, The results shows that there are three families of insectivorous plants founded in study sites, namely Nepenthaceae, Droseraceae and Lentibulariaceae, Six species of Nepenthaceae were found (four species and two natural hybrids) i,e; N. gracilis, N. ampullaria, N. rafflesiana, N. reinwardtiana, N. x hookeriana and N. x trichocarpa. While from family Droseraceae and Lentibulariaceae were found only one species respectively, which is Drosera burmanni and Utricularia bifida. Nepenthes reinwardtiana has the highest rate of photosynthesis namely 21,05 Âµmol/m /s, followed by N. gracilis (17,66 Âµmol/m /s), N. rafflesiana (16,6 Âµmol/m /s), N. x hookeriana (15,84 Âµmol/m /s) and N. ampullaria (9,96 Âµmol/m /s).

ANALISIS IMUNOGENISITAS PROTEIN GRA1 DARI HASIL KLONING GEN GRA1 TAKIZOIT Toxoplasma gondii [Immunogenicity Analysis of GRA1 Protein Derived from Clone Bearing GRA1 Genes Collected from Toxoplasma gondii Tachyzoite] Subekti, Didik T; Artama, WT; Poerwanto, SH; Sulistyaningsih, E; Sari, Yulia
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was aimed to analyze the immunogenicity of GRA1 protein derived from clone bearing GRA1 genes from local isolate of Toxoplasma gondii. Analysis of GRA1 protein translated from cDNA of GRA1 is very essential in prior to expressed the gene. Analysis of GRA1 protein derived from clone bearing GRA1 genes was performed using several bioinformatics software which are available as standalone or online software such as CLC Bio Workbench series, BioEdit, BESTORF, GENSCAN, FGENES, BepiPred 1.0, CTL Epitope Finder and SignalP. Translation coding sequences of GRA1 gene into GRA1 peptide sequences revealed 190 amino acids with molecular mass of GRA1 approximately 20.159 kD and isoelectric point at 4.43. GRA1 protein also identified several antigenic domains with six domains were known as epitopes for CD8+/cytotoxic lymphocyte and seven domain as epitopes for B lymphocyte. However, GRA1 protein was considered as good antigen but less immunogenic.

PENGARUH BEBERAPA PAKET PEMUPUKAN DAN AMELIORASI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KACANG TANAH (Arachis hypogaea L.) DI KAWASAN PENGEMBANGAN LAHAN GAMBUT [Effect of Amelioration and Fertilization Packages on Growth and Yield of Peanut (Arachis hypogaea L.) in Peat Land Development Area] Nurzakiah, Siti; Koesrini, Koesrini; Anwar, Khairil
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Wetlands in Peatland Development Area has potential for the development of paddy, vegetables and palawija. Development of the commodities are limited or restricted by many limiting production factors such as nutrients, water and plant varieties. The purpose of this study is to know the influence of amelioration and fertilization packages on the growth and yield of peanuts in the swampland peat land development area. The treatments consisted of four amelioration and fertilization package i.e.: (1) Farmerâs package I: 250 kg.ha dolomite, (2) -1 -1 -1 -1 Farmerâs package II: 2.5 t.ha straw compost+18 kg.ha P2O5+300 kg.ha fly ash, (3) Recommendation package: 1.0 t.ha dolomite+22.5 -1 -1 -1 -1 -1 -1 kg.ha N + 36 kg.ha P2O5, and (4) Research package: 2.0 t.ha dolomite+22.5 kg.ha N+22.5 kg.ha P2O5 +30 kg.ha K2O. The result showed that the growth of plants (plant height and number of branches) does not differ between treatments, and the obtained peanut pod yield improvement is 50.45% in the research package treatment compared to Farmerâs package I.

SEBARAN DAN HABITAT KUKANG JAWA (Nycticebus javanicus) DI AREA PERKEBUNAN SAYUR GUNUNG PAPANDAYAN, KABUPATEN GARUT [Distribution and Habitat of Javan low loris (Nycticebus javanicus) in Vegetables Gardens at Mount Papandayan, Garut District Area] Wirdateti, Wirdateti
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1887

The need of medicine product are increasing now. The fermentation of rice by using Monascus purpureus produce lovastatin, pigments and citrinin as secondary metabolites. The aim of this study was to know the concentration of lovastatin, red and yellow pigments and citrinin in rice which fermented by Monascus purpureus that had been mutated by using ultraviolet irradiation.Three strains were used in this research, such as AsK, yellow Jmba and red Jmba.The result were analyzed by using HPLC and Spectrofotometer. The result showed that the yellow Jmba strain which treated by UV mutagenesis for 3 minutes contain higher lovastatin (1.798%) and pigment and lower citrinin (0.019%) compare to without mutagenesis. On the other hand ASK strain produced higher lovastatin (1.903%) but higher citrinin (1.270%).This research is expected to provide scientific information to the public. That M. purpureus contained lovastatin that is beneficial, red and yellow pigments for natural food colorant.

POTENSI Enterolobium cyclocarpum (Willd.) Griseb DAN Centrosema pubescens Benth. SEBAGAI AKUMULATOR PENCEMAR MERKURI [POTENCY OF Enterolobium cyclocarpum (Willd.) Griseb AND Centrosema pubescens Benth. AS MERCURY ACCUMULATORS] Hidayati, Nuril
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Some plant species growing in the contaminated areas, indicated high tolerance and potentially affective in accumulating pollutants. These plants can be utilized as hyper accumulators for cleaning up contaminated sites. This research aims to examine plants that grow under Hg contaminated media and to analyze their potency as hyper accumulators. Two pioneer plant species Enterolobium cyclocarpum (Willd.) Griseb and Centrosema pubescens Bent. that proven tolerant and dominant at contaminated sites of gold mine were examined in this research. The plants were grown in liquid media added with mercury (HgCl2) with different level of concentrations 0 ppm, 10 ppm and 20 ppm. Chelate Ammonium Thiosulphate [(NH4)2S2O3] was added with concentration levels of 0 ppm and 20 ppm. Plant performances, cellular responses, Hg accumulation in the plant were assessed. The results revealed that the growth of both plant species decreased with the increase of Hg contamination level. The presents of chelating agent improved adaptability of the plants, indicated by the increase of biomass production and Hg content in the plants, even at the highest level of Hg concentration (20 ppm). Cellular responses showed at 20 ppm Hg, indicated by the decrease of both the number and the size of epidermis and pith cells. Accumulation of Hg in the plants increased with the increased of Hg concentration in the media. Mercury accumulation in both plant species were more concentrated in the root system rather than the shoot.

ANALISA KANDUNGAN LOVASTATIN, PIGMEN DAN CITRININ PADA FERMENTASI BERAS IR-42 DENGAN MUTAN Monascus purpureus [Analysis of Lovastatin, Pigments and citrinin in Rice which fermented by Monascus purpureus mutant] Nurhidayat, N
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The need of medicine product are increasing now.The fermentation of rice by using Monascus purpureus produce lovastatin, pigments and citrinin as secondary metabolites. The aim of this study was to know the concentration of lovastatin, red and yellow pigments and citrinin in rice which fermented by Monascus purpureus that had been mutated by using ultraviolet irradiation.Three strains were used in this research, such as AsK, yellow Jmba and red Jmba.The result were analyzed by using HPLC and Spectrofotometer.The result showed that the yellow Jmba strain which treated by UV mutagenesis for 3 minutes contain higher lovastatin (1.798%) and pigment and lower citrinin (0.019%) compare to without mutagenesis.On the other hand ASK strain produced higher lovastatin (1.903%) but higher citrinin (1.270%).This research is expected to provide scientific information to the public.That M. purpureus contained lovastatin that is beneficial,red and yellow pigments for natural food colorant.

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