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BERITA BIOLOGI

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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 30 Documents

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Search results for , issue "Vol 12, No 2 (2013)" : 30 Documents clear

CENDAWAN PENYEBAB ABORTUS DALAM ALAT REPRODUKSI SAPI BETINA [Fungi Causing Abortion in Reproductive Track of Cow] Gholib, Djaenudin; Ahmad, Riza Zainuddin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study on cases of abortion in cow caused by fungi have never been reported so far in Indonesia. The aim of this research is to get prevalence of cows have not and have aborted, both have reproductive disorder, and health cattles associated with percentage of fungi isolated.The study included both field survey and laboratory examination of samples. The samples of vaginal fluid were collected by using cotton swabs in cow which had suffered abortion or cow had repeated breeding, as well as freeze dried semen for artificial insemination (AI), and also examining the occurrence of abortion in cattle. The samples were collected in Sukabumi and Bandung, and then cultured in Sabouraud Dextrose Agar by stretch method, followed by incubation at 37oC. Number of samples collected consisted of 56 samples of cattle which had suffered from abortion and cow that had reproductive problems; 38 samples as control; five samples of freeze dried semen. There were no cases of abortion observed when field survey was conducted, therefore organ samples were not available for pathological and histophathological examination. Further examination on growth rate of yeast showed 20% of yeast presented in cows with abortion, 17% in cows with reproductive problem and 14% in normal cattle. On the other hand,there were samples that did not show any growth of yeast recorded f rom cows with abortion (15%)cattle with reproductive problem (7%) and normal cows (27%). Meanwhile, only twowith 2 yeast growth observed in semen samples. It was concluded that samples from cattles with reproductive problems (with and without aborted experiences) showed higher number of growth of yeasts compared with normal cow (P<0,05). Freeze dried semen were having risk of contamination by yeasts. There was no different on growth rate of fungi between reproductive problem and with abortion cow (P>0,05).

KEMAMPUAN BEBERAPA JAMUR TANAH DALAM MENGURAIKAN PESTISIDA DELTAMETRIN DAN SENYAWA LIGNOSELULOSA [The Ability of Some Soil Fungi on Degradation of Deltamethrin and Lignocelluloses] Subowo, YB.
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Some of soil fungi capable in degrading pesticide deltamethrin, therefore they can be used as fertilizer in organic farming. As a biofertilizer ,fungus also must be able to provide nutrients for plants. The purpose of the study was to obtain fungal isolates that have the ability to decompose pesticides deltamethrin and lignocellulose compounds, dissolved inorganic phosphate compounds and produce growth hormone IAA (Indole Acetic Acid) . The fungal isolates will then be used in the manufacture of bio-fertilizers. Soil samples were put into mineral medium containing deltamethrin, fungus that grew on this medium would then be isolated. The ability of fungus in decomposing deltamethrin was observed using GC. While the ability of fungi to decompose lignocellulose, to dissolve inorganic phosphate compounds and to produce IAA was observed using a spectrophotometer. The results showed that the fungus Aspergillus niger TR1 had the ability to degrade 90.68% of deltamethrin 500 ppm after 1 h incubation and to degrade 0.46% of Poly R - 478 by after 30 min incubation. In addition, this fungus had cellulase activity of 0.029 units/ml and were able to dissolve inorganic phosphate compounds and produce IAA.

SUBSTITUSI TEPUNG BUNGKIL KEDELAI DENGAN TEPUNG DAUN LAMTORO DAN PENGARUHNYA TERHADAP EFISIENSI PAKAN DAN PERTUMBUHAN IKAN NILA [Effect of Soybean Meal Replacement with Leucaena leucocephala Meal in Pelleted Feed on feed efficiency and the growth of Nile Tilapia] Kurniasih, Titin; Rosmawati, Rosmawati
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The high cost of fish feed is a main problem in aquaculture industries, and the effort to improve the cost efficiency of feed is the main focus for nutritional research program all over the world. Research is needed to identify less expensive and more sustainable ingredients within fish feeds, while maintaining nutritional quality equal to those based mainly on fish meal and soybean meal. Leucaena leaf meal (LLM) is a potential alternative plant protein source because of its high protein content and low cost compared to soybean meal. The Leucaena leaf was soaked in freshwater for 3 days prior to use. This study was a 60-day trial and aimed to examine the replacement of soybean meal with LLM at various level in practical feed for Nile tilapia. This trial used a Completely Randomized Design which was carried out in 15 aquarias sized of 60 x 60 x 50 cm. Triplicate group of nile tilapia (initial body weight 12,04 ÃÂ± 0,38 g fish-1 ) were fed five isonitrogenous and isocaloric feeds. The control feed was containing soybean meal, whereas in other four feeds, LLM directly replaced soybean meal with the inclusion level of 8, 16, 24 dan 31 % within the feed formulation. Result showed that the growth performance and feed efficiencies of feed containing 8, 16, 24 and 31 % LLM on the formulation were significantly lower compared to control feed. LLM was able to consumed by nile tilapia although there was a tendency of decrease of the feed consumption with the increase of LLM inclusion. It suggested that the additional treatment is needed to improve the quality of LLM, and to increase the inclusion level of LLM in feed.

PENINGKATAN PERFORMA REPRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus) PADA MUSIM KEMARAU MELALUI INDUKSI HORMONAL [Reproduction Improvement on Female Striped Catfish Pangasianodon hypophthalmus at Dry Season By Hormonal Induction] Tahapari, Evi; Dewi, Raden Roro Sri Pudji Sinarni
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Striped catfish (Pangasianodon hypophthalmus) spawning success is strongly influenced by environment.Striped catfish spawning generally occurs during the rainy season while in the dry season is difficult to find mature female. This research was conducted to improve reproduction performance of female striped catfish during dry season through combination injection of hormone pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG).The treatments were A : control (no hormone injections), B : injection of PMSG 10 IU/kg + HCG 10 IU/kg and C : injection of PMSG 20 IU/kg + HCG 10 IU/kg.Injections were done at interval of two weeks as many as six times. The research was conducted during dry season in June-October 2012 with rainfall 0.20- 14.40 mm/years. The results showed combination injection of PMSG + HCG could improve female reproduction performance. The best treatment was shown on C treatment with values of OSI (ovi somatic index), fecundity, fertilization rate, hatching rate and larva production was 12.18 %, 523 000 eggs/female, 79.65 %, 69.93 % and 290 000 larvae/female respectively. As for control treatment (A) the value of OSI, fecundity, fertilization rate, hatching rate and larval production, was 6.45 %, 197 000 eggs/female, 48.55 %, 35.59 % and 34 000 larvae/female, respectively

POLA PENYEBARAN, KELIMPAHAN DAN ASOSIASI BAMBU PADA KOMUNITAS TUMBUHAN DI TAMAN WISATA ALAM GUNUNG BAUNG JAWA TIMUR [Distribution Pattern, Association and Abundance of Bamboo in Plants Community in Mount Baung Natural Tourism Park East Java] Sofiah, Siti; Setiadi, Dede; Widyatmoko, Didik
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

One of bamboo forests which are located in a conservation area in Indonesia is Mount Baung Natural Tourism Park, East Java. Bambooforest is a uniqueness/distinctiveness in this area. Study of bamboo ecology in Mount Baung Natural Tourism Park was very important inconservation purposes. The objectives of the research were to assess the distribution pattern, association and abundance of bamboo species at Mount Baung Natural Tourism Park. A systematic quadrat method was used in this study. The distribution pattern of bamboo was calculated using Morisita Index by calculating the Chi-square formula while plant association was calculated using the contingency table. The results indicated that: (1) there were 11 plant species (bearing the Importance Value Index >10%) associated with bamboo, and showing a clumped distribution pattern, (2) bamboo supported the highest importance value, in which Bambusa blumeana was the dominant. The population structure of bamboo in this area showed pre-reproductive phase, indicating the dominance of young stage (D clump < 5m).

KOMPOSISI JENIS DAN CADANGAN KARBON DI HUTAN TROPIS DATARAN RENDAH, ULU GADUT, SUMATERA BARAT [Species Composition and Carbon Stock in Tropical Lowland Forest, Ulu Gadut, West Sumatra] Suwardi, Adi Bejo; Mukhtar, Erizal; Syamsuardi, Syamsuardi
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.529

Tropical lowland forest is one type of forest ecosystem that dominated most of Sumatra areal and retained carbon terrestrial within.Tropical lowland forest has the highest risk of damage than other forest types. The aimed of study is to determine species composition and carbon stock in tropical lowland forest, Ulu Gadut, West Sumatra. This study was conducted on June to October 2012 at 1 ha Pinang-Pinang permanent plot. Tree biomass was measured by using the non destructive sampling method. All trees with stem diameter at breast height (dbh) = 8 cm were measured diameter and were recorded the species of trees.As much as 852 individuals of trees, which were consisting of 45 families and 155 species with DBH = 8 cm were found in Pinang-Pinang permanent plot. Nephelium juglandifolium Blume, Swintonia schwenckii (T. & B.) Kurz,Syzygium sp.,Microcos florida (Miq.) Burret, Palaquium sp.,Cleistanthus glandulosus Jabl., Hopea dryobalanoides Miq., Mastixia trichotoma Blume, Calophyllum soulattri Burm. f. and Shorea maxiwelliana King were dominant based on -1 -1 Importance Value Index(IVI). Trees biomass and carbon stock in the study site are around 482.75 ton ha and 241.38 ton C ha respectively.

IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU [Identification of Jembrana Disease Virus by Using a Molecular Marker of env SU Gene in Bali Cattle] Indriawati, Indriawati; Margawati, Endang Tri; Ridwan, Muhammad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of Â± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.

INDEKS VEGETASI DARI CITRA SATELIT ALOS UNTUK MEMPERKIRAKAN CADANGAN KARBON ATAS PERMUKAAN DI HUTAN MANGROVE [Vegetation Index from ALOS to Predict Above Ground Carbon Stock in Mangrove Forest] Suyadi, Suyadi; Ulumudin, Y.I.; Vebriansyah, R
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mangrove forests sequestrate and store a lot of carbon and are important to tackle climate change. However, much of these forests have been cleared dramatically and such clearings destroyed carbon sinks and released carbon into the atmosphere as carbon dioxide. The international climate agreements highlight Reduced Emissions from Deforestation and Forest Degradation (REDD+) as a key and effective option for mitigating climate change. To help making REDD+ a reality, an alternative approach is needed to measure above ground carbon stock quickly and accurately. Here, we link ground-based data collected from field measurement using belt transect method with satellite image data of the ALOS AVNIR-2. The objectives are to identify the characteristics of mangrove vegetation, to estimate the amount of above ground carbon stock, and to examine capability of vegetation indices of NDVI, SR, dan SAVI from the ALOS AVNIR-2 to estimate the amount of above ground carbon stock in mangrove. Results showed that the mangrove vegetation in Gugus Pulau Pari was relatively diverse and this forest contained above ground carbon between 4694.35 and 42841.25 kg/m2. The statistics analyses showed that there was no significant correlation between the vegetation indices of NDVI, SR, and SAVI with the amount of above ground carbon stock in tropical mangrove forest. In short, the vegetation indices of NDVI, SR, and SAVI from the ALOS AVNIR-2 were not sufficient to estimate the amount of above ground carbon stock in tropical mangrove forest.

NEROLIDOL, KOMPONEN KIMIA AROMATIK TANAMAN TEH YANG JUGA DIPRODUKSI OLEH JAMUR ENDOFIT Schizophyllum sp. D [Nerolidol, an Aromatic Chemical Constituent of Tea Plant that Produce by an Endophytic Fungi Schizophyllum sp. D] Agusta, Andria
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Cultivation of an endophytic fungus Schizophyllum sp.D, isolated from a tea plant Camellia sinensis (L.) O.K. in liquid medium PDB, on a rotary shaker at 100 rpm at room temperature (26 - 30 oC) produced a major metabolite with a production capacity of 56.7 mg/L. Structure elucidation based on MS, NMR spectra and published data showed that metabolite is nerolidol. This chemical compounds is one of important aromatic chemical constituent that contribute to tea aroma and defense system in a tea plant.

KEANEKARAGAMAN GLOMEROMYCOTA DI KEBUN RAYA BATURADEN DAN SEKITARNYA DI GUNUNG SLAMET [Diversity of Glomeromycota in Baturaden Botanic Garden and its Surrounding Area in Slamet Mountain] Kramadibrata, Kartini
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The occurrence of arbuscular fungi (AF), a member of Glomeromycota associated with plant in Baturaden Botanic Garden and its surrounding area in Slamet mountain in Central Java was studied. Part of the Botanic Garden is Agathis plantation which was planted for more than 50 years. Soil samples were collected from rhizosphere of Agathis, wild plants and shrub. In order to obtain spores of arbuscular fungi, dried soil samples were wet sieved and decanted. Part of soil samples were pot cultured with Pueraria phaseoloides. The result showed there were eleven species of AF identified from Baturaden Botanic Garden i.e. three species of Acaulospora, one species of Claroideoglomus, one species of Funneliformis, one species of Glomus, one species of Gigaspora, three species of Scutellospora and one species of Sclerocystis. In this study there are two new records of AF in Java i.e. Scutellospora biornata, and S. erythropus, and also new distribution area for S.projecturata.

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