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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 30 Documents

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Search results for , issue "Vol 12, No 2 (2013)" : 30 Documents clear

ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers] Poerba, Yuyu Suryasari; Ahmad, Fajarudin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.

MORTALITAS DAN PROFIL HEMATOLOGI MENCIT YANG DIINFEKSI Trypanosoma evansi ISOLAT BANGKALAN, PEMALANG DAN PIDIE [Mortality and Haematology Profiles of Mice Infected with Trypanosoma evansi from Bangkalan, Pemalang and Pidie Isolates] Subekti, Didik T; Febria, Mutiara; Sari, Febiola Rama; Hartiyati, Indri N
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.531

Trypanosoma evansi known as trypanosomiasis causes or called surra in animals. Trypanosomiasis-associated death were generally due to severe anemia. Another report mentioned that a rapid death of mice can be attributed to the several reasons. One of those reasons is highly parasitaemia that leads to death of the host. However, there is another distinct report that high parasitemia in mice does not necessarily lead to anemia and clinical symptoms. This research was focused to study the relationship between trypanosomiasis-associated death with haematology profile, particularly the decrease of PCV (packed cell volume) value, anemia and virulence of T. evansi collected from several region of Indonesia. The experiments were performed by dividing mice into four groups. Three groups were infected by T.evansi according to the original isolates while another group were uninfected mice. Every two-day interval, all mice were observed their mortality and parasitaemia and also examined the PCV value and erythrocyte counts in peripheral blood. The experimental results shown that the degree of parasitemia in mice were not always related to mice mortality and anemia. Decreases of PCV value were related to the existence of parasitaemia but not with anemia. This study suggests that there is no relationship between the decreases of PCV value and anemia to the trypanosomiasis-associated death in mice.

PERAN GAMBUT TERHADAP NITROGEN TOTAL TANAH DI LAHAN RAWA [The Role of Peat on Total Nitrogen in The Wetland Soils] Fahmi, Arifin; Radjagukguk, Bostang
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Peatland has important role in wetland ecosystem stability. Depletion and disappearance of peat layer lead to loss of potential source of nutrient and disruption of wetland ecosystem stability. The research aimed to study the influence of peat thickness and land hydrological condition on the total nitrogen (N) content in soil, the influence of rapidly or naturally the depletion and disappear of peat layer on total N content in soil, and the influence of hydrological condition on total N content in soil. Total N was observed in June of 2009 and 2010 (transition from wet to dry season), September of 2009 and 2010 (peak of dry season), and January of 2010 and 2011 (peak of wet season). The research was carried out on potential acid sulphate soil (A), peaty acid sulphate soil (B),shallow peat which all of peat layers wereremoved (C), shallow peat which peat layer was partially removed (D), shallow peat (E), moderate peat (F) and deep peat (G). The results showed that total N content increased with increasing of peat thickness, the depletion and disappearance of peat layer reduced total N content in soil, and declining in the groundwater level increased total N content in soil.

EVALUASI KUALITAS BEBERAPA GENOTIPE BAYAM (Amaranthus sp) PADA PENANAMAN DI JAWA BARAT [Quality Evaluation of Some Genotype of Spinach (Amaranthus sp.) Cultivated in West Java] Rahayu, Suwarni Tri; Asgar, Ali; Hidayat, Iteu M; Kusmana, Kusmana; Djuariah, Diny
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.527

Spinach is one of leafy vegetables species that contain lots of vitamins and minerals. This study aims to examine the quality of several genotypes of spinach grown at two highland sites of West Java (Cipanas and Lembang).This experiment was conducted using Split Plot design,with five lines and five replications. Three genotype were tested, namely B1 = By16 , B2 = By18 , and B3 = By21, as well as two commercial varieties on the market spinach (B4 = red Giti , B5 = Kusuma).Measurements included physical parameters (length and width of leaves,stem diameter, texture, and color) and chemical parameters (water content, vitamin C, and fiber). The experiment was conducted in the laboratory of Physiology Vegetable Research Institute, Lembang from March to September 2012.The results showed that the content of vitamin C and fiber in genotype B1 and B2 were not significantly different from the control genotype.Similarly, the results of measurements of the length and width parameters of the leaves on all the tested genotype was not significantly different from the control genotype. Average length and width leaves of the genotype tested in Cipanas significantly higher than those tested in Lembang. Spinach has a shelf life of 1 day at room temperature in the storage area of Lembang ( 21Â±2Â°C and 85% RH ).

EVALUASI KANDUNGAN PROTEIN DAN SIFAT PASTA BERAS JAPONICA DENGAN MARKA DNA Lestari, Puji; Koh, Hee Jong
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

An evaluation of protein content and pasting properties of rice using molecular markers approach is still limited. This study was aimed to evaluate protein content and pasting properties of japonica rice using DNA marker in complement to conventional tools. This study was further analysis based on the data of protein content and pasting properties of 22 japonica varieties and genotyping data using 30 DNA markers. Multiple regression analysis was performed to formulate equations to estimate the protein content and pasting properties. As a result, the protein content and Rapid Visco Analyzer (RVA) pasting properties values of 22 varieties demonstrated in the range of japonica rice with high variation. Correlation between protein content and RVA pasta properties showed differential profiles. Several type of DNA markers tested in this study showed allele variation among varieties. To estimate the protein content and several parameters of pasting properties of japonica rice, six marker sets in the form of model equations were successfully developed. High resolutions of equations with signifikant R2 in range of 0.95 - 0.99 showed their potency to predict rice physicochemical properties. These developed marker sets are an initial step which needs further validation in order to be able for evaluation of thephysicochemical properties in early generation of breeding and germplasm collection of japonica rice in the future.

IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU Indriawati Indriawati; Endang Tri Margawati; Muhammad Ridwan
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.534

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of ± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.

INDEKS VEGETASI DARI CITRA SATELIT ALOS UNTUK MEMPERKIRAKAN CADANGAN KARBON ATAS PERMUKAAN DI HUTAN MANGROVE Suyadi Suyadi; Y.I. Ulumudin; R Vebriansyah
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.539

Mangrove forests sequestrate and store a lot of carbon and are important to tackle climate change. However, much of these forests have been cleared dramatically and such clearings destroyed carbon sinks and released carbon into the atmosphere as carbon dioxide. The international climate agreements highlight Reduced Emissions from Deforestation and Forest Degradation (REDD+) as a key and effective option for mitigating climate change. To help making REDD+ a reality, an alternative approach is needed to measure above ground carbon stock quickly and accurately. Here, we link ground-based data collected from field measurement using belt transect method with satellite image data of the ALOS AVNIR-2. The objectives are to identify the characteristics of mangrove vegetation, to estimate the amount of above ground carbon stock, and to examine capability of vegetation indices of NDVI, SR, dan SAVI from the ALOS AVNIR-2 to estimate the amount of above ground carbon stock in mangrove. Results showed that the mangrove vegetation in Gugus Pulau Pari was relatively diverse and this forest contained above ground carbon between 4694.35 and 42841.25 kg/m2. The statistics analyses showed that there was no significant correlation between the vegetation indices of NDVI, SR, and SAVI with the amount of above ground carbon stock in tropical mangrove forest. In short, the vegetation indices of NDVI, SR, and SAVI from the ALOS AVNIR-2 were not sufficient to estimate the amount of above ground carbon stock in tropical mangrove forest.

NEROLIDOL, KOMPONEN KIMIA AROMATIK TANAMAN TEH YANG JUGA DIPRODUKSI OLEH JAMUR ENDOFIT Schizophyllum sp. D Andria Agusta
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.530

Cultivation of an endophytic fungus Schizophyllum sp.D, isolated from a tea plant Camellia sinensis (L.) O.K. in liquid medium PDB, on a rotary shaker at 100 rpm at room temperature (26 - 30 oC) produced a major metabolite with a production capacity of 56.7 mg/L. Structure elucidation based on MS, NMR spectra and published data showed that metabolite is nerolidol. This chemical compounds is one of important aromatic chemical constituent that contribute to tea aroma and defense system in a tea plant.

KEANEKARAGAMAN GLOMEROMYCOTA DI KEBUN RAYA BATURADEN DAN SEKITARNYA DI GUNUNG SLAMET Kartini Kramadibrata
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.535

The occurrence of arbuscular fungi (AF), a member of Glomeromycota associated with plant in Baturaden Botanic Garden and its surrounding area in Slamet mountain in Central Java was studied. Part of the Botanic Garden is Agathis plantation which was planted for more than 50 years. Soil samples were collected from rhizosphere of Agathis, wild plants and shrub. In order to obtain spores of arbuscular fungi, dried soil samples were wet sieved and decanted. Part of soil samples were pot cultured with Pueraria phaseoloides. The result showed there were eleven species of AF identified from Baturaden Botanic Garden i.e. three species of Acaulospora, one species of Claroideoglomus, one species of Funneliformis, one species of Glomus, one species of Gigaspora, three species of Scutellospora and one species of Sclerocystis. In this study there are two new records of AF in Java i.e. Scutellospora biornata, and S. erythropus, and also new distribution area for S.projecturata.

EVALUASI KANDUNGAN PROTEIN DAN SIFAT PASTA BERAS JAPONICA DENGAN MARKA DNA Puji Lestari; Hee Jong Koh
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.526

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