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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 14 Documents
 1   2 
Search results for , issue "Vol 7, No 5 (2005)" : 14 Documents clear
PERBANYAKAN TANAMAN BUAH NAGA BERDAGING BUAH MERAH {Hylocereus costaricensis) MELALUI TEKNIK KULTUR JARINGAN Priyono, Priyono
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.379 KB) | DOI: 10.14203/beritabiologi.v7i5.878

Abstract

Hylocereus costaricensis is new important fruit in Indonesia. One of constrain in its development is limitation of planting material.The aim of the research is to study the regeneration H. costaricensis through micro shoot induction of node explants. The experiments were carried out in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute. Experiment on microshoots proliferation stage was arranged in a Factorial Completely Randomized Design, with three replications.The first factor was Kinetin concentration consisted of five treatments i.e.: 0, 2.5, 5.0, 7.5 and 10.0 mg/1.The second factor was Indole acetic acid (IAA) concentration consisted of five levels i.e.: 0, 0.25, 0.5, 0.75 and 1 mg/1. Microshoots multiplication stage was arranged in a Factorial Completely Randomized Design,with three replications. The first factor was polyvinyl pyrrolidone (PVP) concentration consisted of six treatments i.e.: 0, 0.25, 0.5, 0.75, 1.0 and 1.25 %. The second factor was Cystein concentration consisted of four treatments i.e.: 0, 25, 50 and 75 mg/1. The microshoots rooting stage the results experiment was laid in a Factorial Completely Randomized Design, with three replications. The first factor was Giberalic acid (GA3) consisted of five treatments i.e.:0, 0.25, 0.5, 0.75 and 1 mg/1. The second factor was Boric acid concentration consisted of four levels i.e.: 0, 50, 100 and 150 mg/1. In the microshoots proliferation stage the results showed that there was interaction between IAA and Kinetin concentration on the microshoots proliferation and the number of shoot per explnat. The best results were obtained from the treatment 0.75 mg/1 IAA + 7.5 mg/1 Kinetin, whereas in this treatment the rate of microshoots proliferation and the number of microshoots perexplant was 50 % and 3.9, respectively. In the microshoots multiplication stage, the results showed that there was interaction between PVP and Cystein concentration. The best results were obtained from the treatment 0.75% PVP + 75 mg/1 Cystein, whereas in this treatment the rate of microshoots multiplication and the number of microshoots per explant was 95% and 6.3, respectively. In the rooting stage, the results showed that there was interaction between GA and Boric acid concentration. The experiment indicated that 0.5 mg/1 GA3 + 100 mg/1 Boric acid showed the best result to stimulate root induction of the in vitro microshoots propagation, whereas in this treatment the percentage of rooted microshoots and the hight of plantlet were 95% and 5.7 Cm, respectively.
PENGUJIAN SURVIVAL JAMUR YANG DIPRESERVASI DALAM AIR DAN PARAFIN CAIR Suciatmih, Suciatmih; Rachmat, Rachmat
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.754 KB) | DOI: 10.14203/beritabiologi.v7i5.874

Abstract

A study has been conducted for survival testing of 7 and 7-16 years-preserved fungi in water and liquid-paraffin respectively.Survival testing was done through replanting the preserved fungi in new PDA slant media.The growth observation of fungi was carried out for two weeks long.Each collection number was tested with two replications. Thirteen collection numbers of fungi (Alternaria spp.,Aspergillus spp.,Fusarium spp.,Penicillium spp.,and Trichoderma spp.,)which were preserved for 7 and 8 years long in water and liquid-paraffin respectively were growing again, and the rate of survival were 100%. The survival of the fungi(species and sum of sample were different) which were preserved in liquid-paraffin for 7, 8, 9 and 16 years long were 38,5, 81,5,53,6, and 64,7% respectively.
Shorea henryana - JENIS MERANTINON REKALSITRANT Soetisna, Usep; Priadi, Dody
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.985 KB) | DOI: 10.14203/beritabiologi.v7i5.879

Abstract

Shorea henryana - a member species of Dipterocarpaceae known as white meranti. Ecologically distributed on lowland Dipterocarp forest and flourishing in coastal areas. A fairly large tree up to 40 m tall with bole up to 115 cm diameter. Seeds were dispatched from Thailand and showing 72% germination and initial moisture content of 20.3%. First lot of seeds were dried to moisture content 12.5%, 9.4% and 6.1% respectively. Germination of seeds dried to 6.1% m.c. was still relatively high (68%). Contrary to the desiccation resistance, however, the dried seeds seemed not to withstand relatively low temperature for Shorea species, i.e. 14-16 C. Further study will be needed as to investigate a proper storage treatment for this important woody species.
PEMBESARAN IKAN KERAPU BEBEK - Cromileptes altivelis (VELENCIENNES, 1828) DAN IKAN KERAPU LUMPUR - Epinephelus coioides (HAMILTON, 1822) PADA KERAMBA JARING APUNG (KJA) Langkosono, Langkosono
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i5.875

Abstract

Study on the fish rearing of the grouper (Serranidae) as well as barramundi cod grouper - C. altivelis (Valenciennes, 1828) and green grouper - E.coioides (Hamilton, 1822) in floating net cages was carried out from June to December 2004 at the coastal waters of Kodek Bay, Malaka Village, Pemenang District West Nusa Tenggara. This paper discussed the results of both grouper fishes grown in Floating Net Cages culture.
PEMBENTUKAN KALUS DAN EMBRIOGENESIS KULTUR PELEPAH DAUN DAN DAUN Caladium HIBRIDA Irawati, Irawati
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i5.876

Abstract

Petiole and leaf cultures of Caladium hybrid were grown on MS medium+ lppm 2,4-D and transplanted to MS medium + 1 ppm NAA and then were grown on MS media with or without Kinetin and NAA.The greatest capacity of explants to produce callus was from the base cut and the top cut of the petioles as well as from the main vein of the leaf. Compact calli were grew further into adventive buds and plantlets.Through Scanning Electron Microscope,the development of adventive buds were observed grew from the parenchymatous cells and the vascular bundles.
SPECIES RICHNESS AND HABITAT PREFERENCES OF HERPETOFAUNA IN GUNUNG HALIMUN NATIONAL PARK, WEST JAVA [Kekayaan Jenis dan Preferensi Habitat Herpetofauna di Taman Nasional Gunung Halimun] Kurniati, Hellen
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.328 KB) | DOI: 10.14203/beritabiologi.v7i5.877

Abstract

Studi kekayaan jenis herpetofauna (amfibia dan reptilia) telah dilakukan di dalam dan sekitar Taman Nasional Gunung Halimun pada bulan September 2001-September 2002.Metode yang digunakan untuk mengetahui kekayaan jenis adalah dengan pengamatan langsung,penyinaran menggunakan Iampu senter, tangkap langsung dan perangkap lem pada semua habitat herpetofauna di sepuluh lokasi penelitian, yaitu Citalahab, Cibunar, Cianten, Cigadog, Gunung Wangun, Gunung Bedil, Gunung Botol, Legok Karang dan Cikeris.Dari kelompok amfibia didapatkan 25 jenis, sedangkan untuk reptilia 31 jenis; yang termasuk dalam lima suku kodok,empat suku reptilia kaki empat dan empat suku ular. Enam jenis endemik Jawa dijumpai, yaitu Leptophryne cruentata, Microhyla achatina, Huia masonii, Nyctixalus margaritifer, Philautus vittiger, Rhacophorus javanus dan Spenomorphus puncticentralis.Hasil dari penghitungan koefisien indeks kesamaan Jaccard memperlihatkan bahwa sepuluh lokasi survai terkelompok menjadi dua grup besar, yaitu grup hutan dan grup area terganggu.Tipe vegetasi, habitat dan ketinggian tempat merupakan faktor-faktor ekologi utama yang mempengaruhi penyebaran herpetofauna di Taman Nasional Gunung Halimun.
REGENERASI TANAMAN PEPAYA HASIL TRANSFORMASI DENGAN GEN ACC OKSIDASE ANTISENSE [Regeneration of Transforman Papaya Plant with ACC Oxidase Antisense Gene] Purnamaningsih, Ragapadmi; Mariska, Ika; Hutami, Sri
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.521 KB) | DOI: 10.14203/beritabiologi.v7i5.873

Abstract

Papaya is climacteric fruit. As the other climacteric fruit, papaya has hight speed ripening, so papaya fruit can not stored in long period. Genetic enginering is one alternative technology to solve the problem by introducing antisense oxidase ACC gen to the papaya plant genome to get delay ripening characteristic. Success of genetic enginering technology depend on plant regeneration system.There were two ways of plant regeneration: organogenesis and somatic embryogenesis. The aim of this experiment was to induce root formation of papaya planlet which trasformated by ACC oxidase antisense gene.The former experiment showed that explant which transformated by ACC oxidase antisense gene can regenerated to be shoot/planlet with P6 medium.But when the shoot transferred to root induction medium the root was difficult to formed, callus was formed at the base of shoot, the leaves turn to yellow and fall down.Many media formulations were tried in this experiment with different basic medium for root induction and development.MS (1, Vi) DKW (1, A) and WPM (1, Vi) were used as basic media combined with sucrose (2 % and 3 %) and plant growth regulators (kinetin, IAA, and paclobutrazol) adding with some organic compound. Result of the experiment showed that MS Vi + paclobutrazol 0.5 mg/1 induced root formation 80 %, inhibited callus formation and decreased yellowing and falling of the leaves.
PEMBENTUKAN KALUS DAN EMBRIOGENESIS KULTUR PELEPAH DAUN DAN DAUN Caladium HIBRIDA Irawati Irawati
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i5.876

Abstract

Petiole and leaf cultures of Caladium hybrid were grown on MS medium+ lppm 2,4-D and transplanted to MS medium + 1 ppm NAA and then were grown on MS media with or without Kinetin and NAA.The greatest capacity of explants to produce callus was from the base cut and the top cut of the petioles as well as from the main vein of the leaf. Compact calli were grew further into adventive buds and plantlets.Through Scanning Electron Microscope,the development of adventive buds were observed grew from the parenchymatous cells and the vascular bundles.
SPECIES RICHNESS AND HABITAT PREFERENCES OF HERPETOFAUNA IN GUNUNG HALIMUN NATIONAL PARK, WEST JAVA [Kekayaan Jenis dan Preferensi Habitat Herpetofauna di Taman Nasional Gunung Halimun] Hellen Kurniati
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i5.877

Abstract

Studi kekayaan jenis herpetofauna (amfibia dan reptilia) telah dilakukan di dalam dan sekitar Taman Nasional Gunung Halimun pada bulan September 2001-September 2002.Metode yang digunakan untuk mengetahui kekayaan jenis adalah dengan pengamatan langsung,penyinaran menggunakan Iampu senter, tangkap langsung dan perangkap lem pada semua habitat herpetofauna di sepuluh lokasi penelitian, yaitu Citalahab, Cibunar, Cianten, Cigadog, Gunung Wangun, Gunung Bedil, Gunung Botol, Legok Karang dan Cikeris.Dari kelompok amfibia didapatkan 25 jenis, sedangkan untuk reptilia 31 jenis; yang termasuk dalam lima suku kodok,empat suku reptilia kaki empat dan empat suku ular. Enam jenis endemik Jawa dijumpai, yaitu Leptophryne cruentata, Microhyla achatina, Huia masonii, Nyctixalus margaritifer, Philautus vittiger, Rhacophorus javanus dan Spenomorphus puncticentralis.Hasil dari penghitungan koefisien indeks kesamaan Jaccard memperlihatkan bahwa sepuluh lokasi survai terkelompok menjadi dua grup besar, yaitu grup hutan dan grup area terganggu.Tipe vegetasi, habitat dan ketinggian tempat merupakan faktor-faktor ekologi utama yang mempengaruhi penyebaran herpetofauna di Taman Nasional Gunung Halimun.
REGENERASI TANAMAN PEPAYA HASIL TRANSFORMASI DENGAN GEN ACC OKSIDASE ANTISENSE [Regeneration of Transforman Papaya Plant with ACC Oxidase Antisense Gene] Ragapadmi Purnamaningsih; Ika Mariska; Sri Hutami
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i5.873

Abstract

Papaya is climacteric fruit. As the other climacteric fruit, papaya has hight speed ripening, so papaya fruit can not stored in long period. Genetic enginering is one alternative technology to solve the problem by introducing antisense oxidase ACC gen to the papaya plant genome to get delay ripening characteristic. Success of genetic enginering technology depend on plant regeneration system.There were two ways of plant regeneration: organogenesis and somatic embryogenesis. The aim of this experiment was to induce root formation of papaya planlet which trasformated by ACC oxidase antisense gene.The former experiment showed that explant which transformated by ACC oxidase antisense gene can regenerated to be shoot/planlet with P6 medium.But when the shoot transferred to root induction medium the root was difficult to formed, callus was formed at the base of shoot, the leaves turn to yellow and fall down.Many media formulations were tried in this experiment with different basic medium for root induction and development.MS (1, Vi)\ DKW (1, 'A) and WPM (1, Vi) were used as basic media combined with sucrose (2 % and 3 %) and plant growth regulators (kinetin, IAA, and paclobutrazol) adding with some organic compound. Result of the experiment showed that MS Vi + paclobutrazol 0.5 mg/1 induced root formation 80 %, inhibited callus formation and decreased yellowing and falling of the leaves.

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