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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 105   106   107   108   109 
APPLICATION OF MICROSATELLITE MARKERS IN IDENTIFICATION AND PARENTAGE ASSIGNMENT OF SHEEP ET Margawati; Tammen Tammen; M Jones; HW Raadsma
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1220

Abstract

The accuracy of the parentage reference is the early step of information needed for genotyping study.Inaccurate data of recording sheep numbers is often occurred. Sometimes, we found sheep or lambs with uncertain original parents. This study was designed to identify the parentages of sheep and to find the putative parents of lambs. Using an advance DNA technology in molecular genetics particularly in genotyping study, those purposes can be clarified. DNA of sheep were isolated from fresh blood samples in EDTA using a high salt method. A concentration of 50ng/(il DNA was used for amplification. Several microsatellite markers were applied in the amplification. Using a DNA analyser and sequencer, PCR products were analysed to look at the figures of alleles (bands). Results showed that using microsatellite markers could inform that parents of several experimental lambs were incorrect. It was found that putative parents of some lambs were generated from incorrect parents. This study suggests that microsatellite marker can be used to clarify unknown original parents.
LUMUT SEJATI DI KAWASAN CAGAR ALAM GUNUNG PAPANDAYAN GARUT, JAWA BARAT Florentina Indah Windadri
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.675

Abstract

Research on moss diversity in the Papandayan Nature Reserve has been conducted covering the western and eastern areas of the Nature Reserve. Fifty one species of mosses from 14 families and 30 genera were recorded and collected with Dicranaceae as the dominant family.Four species of mosses (Papillaria crocea (Hampe) Jaeg., Barbella rufifolioides (Broth.) Broth., Ctenidium luzonense Broth. and Trichostomum brachydontium Bruch ex Mull.Hal are reported here as new records to Java.
OPTIMASI PRODUKSI FRUCTOSYLTRANSFERASE OLEH Aspergillw sp. WNIC Aris Toharisman; Triantarti Triantarti; Hendro Santoso Marantesa
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2022

Abstract

Fructo Oligo Saccharides (FOS) are considered as biologically benefit, have been developed recently to be used as functional factors in healthy foods.FOS shows low cariogenicity, nondigestibility and proliferation of bifidobacteria in human intestinal tract and dietary fiberlike action. The aim of this research is to produce FOS from sucrose by using fructocyltransferase (FT-ase) from Aspergillus sp. Research on the production of FOS was the optimization of FT-ase production. Inoculum of selected Aspergillus was added into medium with various composition and incubation conditions. Enzyme solution was mixed with sucrose and incubated at various times, pHs, temperatures and agitations.The best parameter condition was based on the highest FT-ase activity. The results showed that production of FT-ase was affected by fermentation time, pH and incubation temperature. The carbon source tested permitted good growth and enzyme production where sucrose supported rather good enzyme production.It was obvious that enzyme production was not closely correlated with cell growth.The best fructo-oligosaccharide yield (20.53%) was achieved when 20 g/100 ml sucrose was utilized. Yeast extract was good nitrogen source for enzyme production. The best FT-ase activity was achieved when 1.2 g/100 ml yeast extract was utilized. Addition of mineral salt also enhanced enzyme production where 1 g/l magnesium salt gave the best cell growth and enzyme production.
FAUNA MOLUSKA PERAIRAN DERAS DI DUA SUNGAI DAERAH RIAU DARATAN M. DJAJASASMITA
BERITA BIOLOGI Vol 3, No 3 (1985)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i3.1356

Abstract

M. DJAJASASMITA. 1985. Molluscan fauna of the two stream rivers of Riau. Berita Biologi 3(3): 121 - 124. - A preliminary study on the composition and population density of the molluscan fauna in the streams of the rivers Tiwi (stony and sandy bottom) and Tandun Kecil (clayed muddy bottom), in Riau, East Sumatra, has been carried out, in July 1977. The molluscs collected consisted of Brotia costula, Clea bocki and Corbicula moltkiana in Tiwi River and B. costula, Pseudodon vondembuschianus and Rectiden graeilis in Tandun Kecil River. B. costula dominate in both localities, 86.08% and 72.58% respectively; the other species range from 2.42% to 25%. In general both localities have low molluscan population density, 41.2 m" and 26.8 m" respectively. The food condition is rather sufficiently available,the water quality may support the molluscan life, the physical condition, however, seems to be less suitable.
ISOLASI DAN SELEKSIJAMUR APHYLLOPHORALES PENGURAI LIGNIN DIHUTAN BUKIT BANGKIRAI, KALIMANTAN TIMUR YB Subowo
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i6.857

Abstract

Isolation and selection of Aphyllophorales as lignin degrading fiingus had been done in Bukit Bangkirai Forest, East Kalimantan. Aim of the research was to obtain Aphyllophorales fungi which have ability to degrade lignin. The fresh fruit bodies were collected from trunks, branches and litters of plants in the forest. The work was conducted at dry season. Fruit bodies were taken to base camp for isolation. The isolates were tested for their ability to degrade lignin. We found 24 isolates of Aphyllophorales fungi. Those isolates were grew in poly R-478 medium, 12 isolates were able to degrade lignin. Megasproporia sp. strain IB-24 had the highest ability in lignin degradation. The fungus can degrade poly R-478 8,05% during 90 minutes.
STRUKTUR BIBIR Osteochilus partilineatus KOTTELAT DAN PERBANDINGANNYA DENGAN 7 JENIS Osteochilus LAINNYA Renny Kurnia Hadiaty
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3454

Abstract

Osteochilus is a genus of fish with large lips,everted, sucker-like, covered with more or less folds of plicae bearing numberous unicellulair keratinous projections or uncuil. The structure of thr lips Osteochilus is divided into three from i.e. ectomorph, mesomorph and endomorph, based on the length and shape of plicae on the lateral portion of the upper lip, the distribution of uncuil and taste buds on the plicae , also from the size and shape of uncuil. Osteochilus partlilineatus is a small fish which has a nice colour patteern, so it is potential as an ornamental fish. it has an ectomorph lips, which the structure of plicae,uncuil and taste buds. The from of the taste buds redemble the rose flower which are very different with the structures of that 7 other Osteochilus that were examined by Roberts using scaning Electro Microscope (SEM).
EFISIENSI PEMANFAATAN RENDEMEN KAYU DAN DEGRADASI POTENSI TEGAKAN EBONI DI HUTAN ALAM Soenarno Soenarno
BERITA BIOLOGI Vol 6, No 2 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i2.1498

Abstract

Sampai sekarang kayu eboni {Diospyros celebica Bakh.)masih tetap diminati banyak kalangan, baik di dalam negeri maupun luar negeri.Jenis kayu ini merupakan tanatnan khas Sulawesi dengan kualitas sangat baik.Karena struktur garis serat dan warna kayu teras yang indah serta sifat kayunya yang awet maka menjadikan kayu eboni bernilai komersial tinggi.Di lain pihak, keberadaan kayu eboni alam tersebut yang dahulu merupakan tulang punggung perekonomian masyarakat di sekitar kawasan hutan, bahkan dijadikan komoditas andalan sektor kehutanan untuk menghasilkan devisa negara.Tetapi, kini kondisi eboni alam dirasakan makin memprihatinkan.Hal tersebut diindikasikan oleh makin sulit/langkanya ditemukan kayu eboni di pasar bebas.Banyak faktor penyebab makin merosotnya potensi eboni alam, di antaranya adalah faktor teknis berupa borosnya tingkat efisiensi pemanfaatan dan rendahnya rendemen kayu teras yang terkandung pada pohon eboni yang ditebang serta faktor non teknis yang berkaitan dengan implementasi kebijakan, penebangan ilegal,lemahnya pengawasan dan rendahnya kesadaran serta dukungan penelitian yang tidak memadai.
DISEMINASI HASIL RISET KEANEKARAGAMAN HAYATI UNTUK MASYARAKAT MELALUI PROGRAM PENDIDIKAN KONSERVASI Tatang Mitra Setia; Herda Pamela Hutabarat; Khoe Susanto Kusumahadi
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1183

Abstract

Indonesia, walaupun sebagai negara yang kaya akan keragaman hayati, namun dewasa ini mengalami berbagai tekanan terhadap keanekaragaman ini.Tekanan-tekanan ini bersumber dari dua masalah utama yaitu pertama, pembangunan yang tanpa memperhatikan keseimbangan alam sehingga telah menimbulkan perubahan yang merusak, dan kedua, meningkatnya populasi (penduduk).Perlu langkah-langkah untuk melestarikan alam dan fungsinya di Indonesia, secara serentak dan lintas sektoral {total football),demi meningkatkan daya dukung alam itu sendiri.Oleh karena itu, pelestarian alam ini harus menjadi pola perilaku hidup.Untuk membentuk pola perilaku ini perlu dilaksanakan 2 program utama yaitu a) pendidikan pelestarian alam dan lingkungan dan b) pendidikan etika dan moral.Pendidikan konservasi dan lingkungan selain sebagai langkah awal dalam strategi pelestarian alam, juga dipandang sebagai salah satu media untuk desiminasi hasil riset dan pengembangan dalam bidang keanekagaraman hayati.Aspek diseminasi dibahas dalam makalah ini.
PERKEMBANGAN EMBRIO DAN LARVA IKAN PATIN NASUTUS (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) Bambang Iswanto; Evi Tahapari
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.622

Abstract

Patin nasutus (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) is a potential pangasiid catfish species to be cultured in Indonesia.Many efforts has been done to explore the potency of P.nasutus for aquaculture purposes.However, basic knowledge is still very rare such as its embryonic and larval development.The present study aimed to understand the embryonic and larval development of P. nasutus resulted from induced spawning of P.nasutus brooders at Research Institute for Fish Breeding, Sukamandi. Those embryonic and larval development were observed microscopically.The results showed that diameter of the ovulated oocytes ranged 1.40-1.65 mm and fertilized eggs ranged 1.90-2.15 mm.The embryogenesis consisted of several stages as follow: morula, blastula, gastrula, organ developments and newly hatched larvae occured within periods of (minutes) 20-200, 190-220, 210-660, 600-1,140 and 1,800-2,160 after fertilization at water temperature of 28-29oC. Total length of the newly hatched larvae ranged 4.80-5.10 mm with anterior, posterior and dorsal parts of the yolk sac coloured black pigmentation. On caudal peduncle, the black spot existed since 36 hours after hatching was a strong character for P.nasutus larvae.Volume of the yolk sac was 2.65±0.14 mm3, for which 50% absorbed at 36 hours after hatching and relative fully absorbed at 78 hours after hatching. The larvae have complete morphology as adult after seven days. During early developmental stage, larvae showed cannibalism since the third day, corresponding to the first exogenous feeding.
UJI DAYA HAMBAT DAUN SENGGANI (Melastoma malabathricum L.) TERHADAP Trichophyton mentagrophytees DAN Candida albicans Djaenudin Gholib
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1989

Abstract

The in vitro study of" inhibition effect of senggani leaves (Melastoma malabathricum L.) ethanol extract on Trichophyton mentagrophytees and Candida albicans was conducted by diffusion and dilution method. The extract was diluted into 5%, 10%,15% and 20% for diffusion test, and 1%, 2%, 3%, 4% and 5% for dilution test on T. mentagrophytees, and 2%, 4%, 6%, 8%, and 10% on C. albicans. The results showed that the extract inhibited fungal growth. Agar diffusion test (agar wheel) was observed by diameter of inhibition zone around the wheel. At 20% extract dilution, the diameter mean was estimated 30 mm on T. mentagrophytees, and 21 mm on C. albicans. In dilution test, the result showed Minimal Inhibition Concentration (MIC) of the extract was 3% on T. mentagrophytees, and 6% on C. albicans. It is concluded that the extract effect of inhibition on T. mentagrophytees is greater than that on C. albicans.

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