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BERITA BIOLOGI
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Articles 2,125 Documents
KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR Prana Mahardhika; Rudhy Gustiano; Dinar Tri Soelistyowati; MH Fariduddin Ath-thar
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.771

Abstract

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 ± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 ±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.
MIKROPROPAGASI TANAMAN ILES-ILES {Amorphophallus muelleri Blume) Maria Imelda; Aida Wulansari; Yuyu S Poerba
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i4.2117

Abstract

In Indonesia, iles-iles (Amorphophallus muelleri Blume) has not yet been cultivated intensively; their cultivation area is still limited. This species contains high glucomannan, which is useful as food diet, paper pulp, textile, paint, film-negative, celluloid and cosmetic industry. The cultivation of A. muelleri is hampered by limited genetic quality of plant.The species is triploid (2n=3x=39), the seed is developed apomictically, and pollen production is low.This may explain that the species is difficult to breed conventionally and genetic variability in the existing landraces cultivars is rather limited. Genetic variability of this plant is therefore can be achieve by induced mutation through tissue cultures for use in breeding program to develop better cultivars.Developing an efficient and effective micropropagation of the species is therefore important for use in the genetic improvement program.In other hands, the prospect for development and export of iles-iles is high since the demand from Japan alone has not been fulfilled. Propagation of iles-iles is generally done by splitting tubers, bulbils or leaf cuttings, but this method can not yield planting materials in large quantities within a relatively short time. In this research, young shoots which had just appeared from tubers were used as a source of explants. Sterilization of the explants was carried out in 0.05 % HgCl, solution for 20 min, rinsed several times with sterile distilled water and then cultured on Murashige and Skoog (MS) medium containing 0.1-0.2 mg/1 Thidiazuron (TDZ), 0.5-1.0 mg/1 Benzylaminopurine (BAP) and 0.5-1.0 mg/1 Kinetin (KIN) singly or in combination. Acclimatization of plantlets was done on 3 kinds of media namely (A), soil + compost, (B) soil + compost.+ cocopeat, and (C) soil + cocopeat. The results showed that the best medium is MS containing 0.2 mg/1 TDZ and 0,5 mg/1 BAP for in vitro shootbuds induction and proliferation of iles-iles, while MS without plant growth regulators is suitable for shoot growth and root formation and soil + compost + cocopeat for acclimatization of plantlets.
SIFAT-SIFAT SEMAI DOLICHOS TRILOBUS, LABLAB PURPUREUS DAN MACROTYLOMA UNIFLORUM (LEGUMINOSAE) N. WULIJARNI; SOETJIPTO SOETJIPTO
BERITA BIOLOGI Vol 2, No 6 (1981)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v2i6.1402

Abstract

Dari hasil penelitiannya mengenai marga Dolichos L. dan kerabatnya dari Aftika timur. Verdcourt (1970b) telah memisahkan Dolichos lablab L. dan Dolichos uniflorus Lam. dari margaDolichos dan menempatkannya berturut-turut dalam marga Lab-lab Adans. dan Macrotyloma (Wight & Arn.) Verde. Sebagai konsekuensinya D. lahlab diganti namanya menjadi Lablab purpureus (L.) Sweet dan merupakan jenis tipe marga Lablab yang hanya terdiri atas satu jenis, sedang D. uniflorus menjadi Macrotyloma uniflorum (Lam.) Verde, dan merupakan tipe marga Macrotyloma. Pemisahan kedua jenis ini dari marga Dolichos didasarkan terutama pada adanya perbedaan dalam sifat-sifat putik, serbuk sari serta kromosomnya (Tabel 1).
THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Atit Kanti; I Made Sudiana; Heddy Julistiono
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1081

Abstract

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.
KONDISI PLANKTON PADA TAMBAK UDANG WINDU (Penaeus monodon FABRICIUS) DENGAN SUBSTRAT BERBEDA Erfan Andi Hendrajat; Andi Sahrijanna
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3496

Abstract

Plankton is one of water microorganisms that plays an important role as natural food for fish and shrimp and as an stability indicator of water or aquatic environment.The purpose of this study was to determine the composition and abundance of plankton in tiger shrimp ponds using concrete ponds without substrate, concrete ponds using sand substrate and ponds. Sampling of plankton and water quality was carried out from March to July 2016 at PunagaTakalar Experimental Pond at 6 ponds consisted of concrete ponds without 2 subplot of sand (pond A) and concrete pond with 2 subplate sand subplate (pond B) respectively measuring 1,000 m2 and a 2-square-meter plot of 2,500–3,000 m2 (pond C). The results showed that plankton abundance in pond A ranged from 37–349 individuals/L, pond B ranged from 35–1,399 individuals/L and pond C ranged from 54–999 individuals/L. The most common phytoplankton genera in this study was Oscillatoria while for zooplankton is of  Acartia.  The diversity of plankton in ponds A, B and C is included in the community of unstable to moderate biota. In general, the diversity of plankton in pond B is included in the community of moderate biota. The plankton uniformity index in pond A, pond B and pond C is generally close to 1 this indicates that the existence of plankton species in the three ponds is relatively even.      
TOKSISITAS AKUT ORAL DUA SENYAWA BISANTRAKUINON (+)-2,2’-EPISITOSKIRIN A DAN (+)-1,1’-BISLUNATIN [Oral Acute Toxicity of Two Bisanthraquinones (+)-2,2’-Epicytoskyrin A and (+)-1,1’-Bislunatin] Praptiwi Praptiwi; Arif Nurkanto; Dewi Wulansari; Andria Agusta
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1858

Abstract

Bisanthraquinones (+) - 2,2'-epicytoskyrin A and (+) -1,1'bislunatin produced by the endophytic fungus Diaporthe sp. GNBP-10 showed potent antibacterial activity on in-vitro test and have the opportunity to become new antibiotics candidates. The aspects of safety and toxicity of drug candidates have to be examined before applying to human. This study was conducted to determine the safety aspects of the compounds through acute oral toxicity testing in mice (Mus musculus). Acute toxicity of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin evaluated by the method of Up and Down Procedure with limit test at a dose of 2000 mg / kg. Results of acute toxicity test showed that the LD50 of (+) - 2,2'-epicytoskyrin A and (+) - 1,1'-bislunatin were of 1638.87 mg / kg and > 2000 mg / kg respectively. Administration of (+)- 2,2'-epicytoskyrin A resulted in increased miliari multifocal hepatitis, fatty degeneration and necrosis of liver cells, and the renal tubule epithelial degeneration. Administration of (+) - 1,1'-bislunatin at a dose of 2000 mg / kg resulted in multifocal accumulation of inflammatory cells in the liver and degeneration of cells in the islets of Langerhans although not resulting in death. The administration of those compounds indicated the changes in the organs, but based on the UN/ECE classification of LD50 value showed that (+) 2,2'- epicytoskyrin A and (+) -1,1'-bislunatin included as low acute toxicity substance.
PENDEKATAN TEKNIS PELESTARIAN EBONI (Diospyros celebica Bakh.) SECARA EX-SITU Ngakan Putu Oka
BERITA BIOLOGI Vol 6, No 2 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i2.1503

Abstract

Pohon eboni (Diospyros celebica) pada hutan alam telah mengalami tekanan eksploitasi yang intensif karena kayunya memiliki nilai ekonomi yang sangat tinggi. Keadaan ini mengakibatkan popuiasi jenis pohon endemik Sulawesi ini menjadi sangat berkurang, sampai pada tingkat yang mengkhawatirkan kelestariannya. Oleh karena itu, D.celebica telah ditetapkan sebagai tumbuhan yang tergolong vulnerable species dan sebagian besar habitat tempat hidupnya ditetapkan sebagai suaka alam atau kawasan yang dilindungi khusus untuk melestarikannya. Pendekatan pelestarian eboni secara in-siiu seperti yang diuraikan di atas belumlah memadai untuk dapat dengan cepat memulihkan popuiasi dan potensi eboni.Selain itu, pendekatan pelestarian sebagaimana tersebut di atas tidak memberikan peluang bagi pemanfaatan kayu eboni pada saat popuiasi dan potensinya telah memungkinkan untuk dimanfaatkan. Untuk mengatasi hal tersebut, perlu dipikirkan beberapa pendekatan pelestarian lainnya, di antaranya adalah pelestarian secara ex-situ.Pendekatan pelestarian secara ex-situ dapat diarahkan pada upaya pengelolaan dan pemanfaatan eboni secara lestari. Namun untuk mengarah pada pendekatan pelestarian secara ex-situ diperlukan telaahan mengenai preferensi ekologis dan karakteristik fisik maupun fisiologis yang terkait dengan bentuk-bentuk pengembangannya. Telaahan mengenai kemungkinan pengembangan beberapa bentuk tanaman D. celebica sebagai upaya pelestarian secara ex-situ dipaparkan.
ISOLASI DAN IDENTIFIKASI Pseudomonas DARI TANAH KEBUN BIOLOGI WAMENA DAN UJI AWAL SEBAGAI AGEN BIOKONTROL Fusarium HJD Latupapua; N Nurhidayat
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1189

Abstract

Pseudomonas bacteria plays essential role in soil ecology such as decomposer and biological control. The bacteria were isolated on selective media and identified from five soil samples taken within area of Wamena Biological Gardens.There are six species Pseudomonas were indentified based on morphological characters and biochemical reaction.P. striata was found to be common in soil of the area.No pathogen Pseudomonas was indentified in all soil samples. Preliminary study on biological control for fungal pathogen Fusarium indicated that P. fluorescens, P. striata and P. cepacia have ability to inhibit Fusarium growth in vitro.
ANALISIS KARBOKSIMETIL SELULOSA DARI BAKTERI Acetobacter xylinum DAN Acetobacter sp. RMG-2 Ruth Melliawati; Apridah Cameliawati Djohan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.642

Abstract

Bacterial cellulose has long been manufactured and used for industrial purposes and health. Bacterial cellulose more profitable than the cellulose plants because during the manufacturing process, they do not harm the environment. The purpose of this study was to identify differences of cellulose produced by Acetobacter xylinum and Acetobacter sp. RMG-2 from that produced by plants. The study was also aimed to determine superiority of carboxymethyl cellulose (CMC) produced by those bacteria. The medium HB was prepared for the production of cellulose from both bacteria. Bacterial cellulose preparation was carried out to obtain solid fine powder, followed by manufacturing carboxymethyl cellulose through several stages to obtain CMC powder. CMC analysis was performed for both bacteria and plants targeted on the surface structure of cellulose, the density of solids, viscosity CMC and functional groups. As a result, the surface fiber cellulose plants had a wider space than fiber cellulose bacterium. The density of solids of CMC A. xylinum, A. sp. RMG-2 and plant were 30.9998 g/cm3, 0.0079 g/cm3 and 0.9978 g/cm3 respectively. Viscosity of the CMC were of 5.78 cP, 5.25 cP and 5.91 cP for each A. xylinum,A. sp. RMG-2 and plant. CMC functional groups of bacteria has met the parameters of success as indicated by the infrared spectrum since it formed a methyl group, carboxyl group and the group of sugar. Cellulose Acetobacter sp. RMG-2 and A. xylinum cellulose can replace plants through the process of compound alkalization with sodium hydroxide, because the compound can lower the level of density of pores of cellulose fibers. The CMC resulting from bacterial cellulose as good as CMC plant and had characteristics resembling CMC plant.
STUDI FITOKIMIA Baeckea frutescens L: PENGARUH FAKTOR LINGKUNGAN TERHADAP KOMPOSISI KIMIA MINYAK ATSIRI Tri Murningsih
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1994

Abstract

Isolation of two essential oils of Baeckea frutescens L. leaves from different locations (Yogyakarta and Semarang) was done by hydrodistilation. The chemical composition of the oils were analysed by GC-MS. The oils were found to possess chemical compositional differences and considerable variation in the levels of each chemical components, suggesting the existence of chemical varieties.The oils were dominated by monoterpene i.e 8-cineole (22,08-22.67%) and P-pinene (17,98-29.22%).Comparison of chemical composition of the oils analyzed in this study and oils from literatures was conducted to study the effect of environmental factor on the essential oil composition.The result showed that temperature, irradiance, photoperiod and altitute factors influence the chemical composition of essential oils. Tasmanone, a non-terpenic triketone only appears in the essential oils from Quang Binh province (north Vietnam), that have extreme environmental condition.

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