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BERITA BIOLOGI
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Articles 2,125 Documents
PENGARUH PEMBERIAN ELISITOR EKSTRAK KHAMIR Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN AJMALISIN DALAM KULTUR AGREGAT SEL Catharanthus roseus (L.) G. Don. Jujun Ratnasari; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1118

Abstract

There were many ways to obtain high production of secondary metabolites in plant tissue culture; among the other is elicitation.An experiment to study the effect of elicitor derived from Saccharomyces cerevisiae Hansen extract on ajmalicine content in cell aggregates culture of Catharanthus roseus (L.) G.Don, has been conducted. The media used for callus induction and cell aggregates culture were Zenk 6 5(1977) with addition of 2.5 x 10" M Naphthalene Acetic Acid (NAA) and 10' M 6-Benzilaminopurine (BAP).The cell aggregates culturewas subcultured three times and then elicitated with elicitor derived from autoclaved 5.cerevisiae extract at concentrations 0.5, 1.0, 2.5%,and harvested at 18, 24,and 36 hours after elicitation.The ajmalicine was analyzed qualitatively and quantitatively by using High Pressure Liquid Chromatography (HPLC) connected to chromatopack CR-7A Plus. The cell aggregates of C. roseus culture produced ajmalicine both in the cells and the media.The result of elicitation showed that ajmalicine content was influenced significantly by concentration and harvesting time. The highest ajmalicine content in the cell aggregates was 25.288 ± 0.102 jig/g dw, whilst that in media was 524.600 ± 0.566 \\ML. The optimum concentration of S. cerevisiae extract was 0.5%, and the best harvesting time was 24 hours.
RESPON PADI TRANSGENIK CV. NIPPONBARE GENERASI T1 YANG MENGANDUNG GEN Oryza sativa DEHYDRATION-RESPONSE ELEMENT BINDING 1A (OsDREB1A) TERHADAP CEKAMAN SALINITAS Tri Joko Santoso; Aniversari Apriana; Atmitri Sisharmini; Kurniawan Rudi Trijatmiko
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.494

Abstract

Salinity is one of the abiotic constraints in the cultivation of rice crop. One of the reasons agricultural land becomes saline is due to the intrusion of seawater into the mainland as a result of global climate change. Dehydration-responsive element binding (DREB) gene is a plant -specific transcription factor gene that have important role in regulating plant responses to abiotic stresses, including high salinity. Transgenic rice plants cv. Nipponbare carrying OsDREB1A gene have been generated. However, study of the response of putative transgenic plants to salinity has not been done. The research objective is to study the response of T1 generation Nipponbare-OsDREB1A transgenic rice plants to salinity stress. The result showed that the response of putative transgenic rice Nipponbare-OsDREB1A to salinity stress 25 mM and 150 mM NaCl indicated a level of tolerance varies from highly sensitive to highly tolerance. These variations were possibly occurred because of the segregation state of the T1 generation transgenic rice. Based on damage symptom scoring and PCR analysis provided information that transgenic rice plant cv. Nipponbare-OsDREB1A which showed positive PCR had a very high tolerance to salinity stress 150 mM compared with non-transgenic rice cv. Nipponbare.
RAGAM FENOTIPE IKAN TENGADAK Barbonymus schwanenfeldii (Bleeker 1854) HASIL SILANG LUAR Firda Amalia Sukma; Muhammad Fariduddin Ath-Thar; Odang Carman; Deni Radona
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3773

Abstract

Outbreed may increase genetic diversity and produce better offspring. Meanwhile, genetic diversity can be inferred from phenotypes variability. This study aimed to analyze the diversity of phenotypic characters from four populations of tinfoil barb outbreed, namely Java (J), Kalimantan (K), ♀ Java × ♂ Kalimantan (JK) and ♀ Kalimantan × ♂ Java (KJ).Analysis of the phenotypic diversity was calculated based on truss morphometric on 30 individuals sampled from each populations. The growth and survival rate were measured for 40 days reared. The study was conducted experimentally using a completely randomized design (CDR) with three replications.Rearing four population of tinfoil barb, beginning with acclimatization for two weeks and was continued reared using 12 aquaria40x30x30 cm3. Each aquaria was filled with 10 individual seeds/20 L-1 water with the size of 18,66±1,34 g weight and 8,28±0,34 cm of length. Feeding was done three times a day on ad-libitum using commercial pellet with protein content of31˗33%. The result of the canonical function analysis of truss morfometrik thetinfoil barb on 21 characteris indicated that the individual morphological characterof population of the tinfoil barb outbreed (JK and KJ) interrelated with each other.The highest sharing component value of inter population was obtained on the fish from outbreed of KJ and JK (13,3%), while the highest of intra population was obtained of K (96,7%) and the lowest of JK (86,7%). The highest survival rate (100%) was recorded on the JK population and significantly different (P>0,05) with survival rate of 90% observed in the J population. 
KOMPOSISI KIMIA MINYAK ATSIRI PADA BEBERAPA TIPE DAUN TEMBAKAU (Nicotiana tabaccum L.) Elda Nurnasari; Subiyakto Subiyakto
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1913

Abstract

Steam distilation of tobacco (Nicotiana tabaccum L) from 4 different area, Madura (1160 gram), Temanggung (2000 gram),Bondowoso (3528 gram), and Blitar (950 gram) yielded 2,67x10-2 %, 6,93x10-2 %, 0,8428%, and 0,0632 % essensial oil respectively.The oils then analyzed using GC-MS methods. The results identified 30 components from the Madura’s tobacco, 11 components from the Temanggung’s tobacco, 67 components from the Bondowoso’s tobacco, and 20 components from the Blitar’s tobacco.The major essential oil compounds was neophytadien.
MORFOLOGI SEMAI BEBERAPA JENIS PALEM HIAS Seedling morphology of some ornamental palms Ning Wikan Utami; Hartutiningsih M Siregar
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1273

Abstract

The results of 18 palms species studied showed that there were three species (Livistona chinensis (Jack) R.Br.ex Mart,Thrinax sp.and Sabal meeacarpg Small) which their cotyledone growth under ground level (group I), their first leaf did not cleave, these had two types of germination i.e. remote tubular (L. chinensis and Thrinax sp.)and remote ligular (S. meeacarpa).Thirthteen species (Heterosphate elata Scheff, Archonthophoenic alexandrae Wendl.& Drude, Areca triandra Roxb., Bentinckia nicobarica (Kurz.) Becc, Chrvsalidocarpus sp., C. lutescens H. Wendl.,Cvrtostachvs eleeans Burret, C.renda Blume, Drvmoploeus pachvcladus H.E.Moore, Neodypsis decarvi Jumelle,Oenocarpus panamanus L.H.Bailey, Pthvchosperma eleeans (R.Br.) Blume and Pritchardia pacifica Seem. & H.Wendl.) produce their cotyledone near ground level (group H), their first leaf cleft into two parts except on the species O. panamanus cleft into four parts, all of these species had adjacent ligular type of germination. There was large variability on the germination.The range of first germination was 12105 days after sowing, last germination was in the range of 78-212 days and the germinationpersentage was 1.3-94.0%.
KERAGAMAN GENETIKA POPULASI PELAHLAR (Dipterocarpus littoralis (Bl.) Kurz) DIPULAU NUSAKAMBANGAN BERDASARKAN PROFIL ENHANCED RANDOM AMPLIFIED POLYMORPHIC DNA Kusumadewi Sri Yulita; Tukirin Partomihardjo
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.772

Abstract

Dipterocarpus littoralis is a commercial timber species endemic to Nusakambangan island. Their existence is under severe threat due to habitat conversion and illegal logging. This study aimed to assess genetic diversity of this species within and among populations using E-RAPD. Three arbitrary RAPD primers were modified by addition of two nucleotides at their 5' termini.E-RAPD profiles were obtained by performing PCR amplification using the modified primers. Forty-five putative loci of E-RAPD were scored and analysed using POPGENE software. Some of the RAPD bands (OPA 9E-200 and 850 bp) were distinctively found for population 2, thus it served as population diagnostic marker for this population. Genetic diversity within population (0.1540) was higher than that of among populations (0.0418). Genetic differentiation (Gst) indicated that 21.35% of total genetic diversity in D. littoralis was attributed to the differences among populations. The highest genetic diversity was found in population 2(He:0.1923; 1:0.3158), while the lowest genetic variation was observed in population 1 (He: 0.0828; 1: 0.1209). Total genetic diversity for all population (Ht) was 0.1958 with an average value of genetic diversity within populations (Hs) was 0.1540. This fact suggested high level of genetic diversity found on these relic populations.
AKAR PENYEBAB DEFORESTASI DI SEKITAR SUNGAI PEMERIHAN PERBATASAN TAMAN NASIONAL BUKIT BARISAN SELATAN, LAMPUNGBARAT Suyadi Suyadi; David Luc Andre Gaveau
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i4.2118

Abstract

The destruction of lowland tropical forests represents one of the greatest threats to biodiversity in Sumatra's third-largest protected area, the Bukit Barisan Selatan National Park (BBSNP). Here, we integrate time-series satellite and socio-economic information in a Geographic Information System (GIS) to unravel the root causes of deforestation in two encroachments(Cawangaro and Sumbersari) near Pemerihan River bordering BBSNP.Cawangaro was originally developed as an enclave in 1965.Sumbersari remained forested until 1978.Both encroachments experienced higher deforestation rates during the late 1970s and in 2005. Our GIS combined with historical records show that large-scale logging, high coffee price and village development plans (perluasan lahan usahatani penduduk) drove deforestation during late 1970s. Throughout the 1980s and until 1997/1998, deforestation rates have been negligible as strong law enforcement measures were adopted by the Government to protect biodiversity during the implementation of Indonesia's first land-use plan (TGHK). Since the 1997-1998 Asian economic crisis, deforestation rates have increased again. Results from socio-economic surveys showed that illegal logging has become one major factor driving agricultural encroachments in Pemerihan since 1998. Local tycoons have exploited the park's timber since Suharto's fall, probably due to the decline of law and order. While timber extraction itself has caused very limited damage to the forest, this illegal activity sparked a land race among farmers inside the park.
THE SUSCEPTIBILITY OF LABORATORY ANIMALS TO TRYPANOSOMA EVANSI INFECTIONS HASAN BASRI MUNAF
BERITA BIOLOGI Vol 2, No 6 (1981)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v2i6.1403

Abstract

Trypanosoma evansi, one of about 200 trypanosomes species encountered in mammals in the world, is the causative agent of an economically important disease among domestic animals called surra. Many studies on T. evansi has been made using various experimental animals such as chicks and fowls for kifectivity (Alwar 1962, Manuel & Cajita 1967), mice for influences of temperature (Marthur 1976) and rats for immunological studies (Assoku 1975). Even larger animals such as goats, pigs, horses, cattle and buffaloes have been used by several workers in studying T. evansi infections (Chand & Singh 1971, Gill 1971, Srivastava & Ahluwalia 1972).The present paper deals with the susceptibilities of small laboratory animals to T. evansi infections in order to determine which animals are suitable for maintaining T. Svansi in the laboratory. All works in this study were done at the Filariasis Research Division of the Institute for Medical Research (IMR), Kuala Lumpur.
FLUKTUASI CURAH HUJAN DAN KOMUNITAS SERANGGA DI HUTAN TROPIS TAMAN NASIONAL GUNUNG HALIMUN*[Fluctuation of rainfall and insect community in a tropical forest, Gunung Halimun National Park] Sih Kahono; Woro A Noerdjito
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1082

Abstract

Study on fluctuation of rainfall and insect community was conducted in a tropical forest, Gunung Halimun National Park. Ten years rainfall data was available to discuss their fluctuations. Rainfall was relatively non-seasonal with high number of almost entire monthly rainfalls.Although fluctuation of rainfall in GHNP did not show clear seasonal trend, however appearance of less rainfall showed from June to September. There were several slight fluctuations in the number of rainfalls from a month to another and some time showed major and minor peaks. Monthly dry months had never reached 0 mm. In ordinary years, only one-month drought occurred in July 1991 (90 mm) and December 2000 (8 mm). Strong drought occurred only during El Nino of 1994 and 1997, which have 3 to 4 dry months. Fluctuation of insect community was studied from March 2000 up to February 2001. Two insect collection methods were applied by setting up light traps and pitfall traps. Total individual of each order of insect counted monthly and to be compared one to another. Changing the number of monthly individual of each order was interpreted to the number of monthly rainfall in order to analyze the relation of changing of the number of rainfall and the individual of each order of the insect collected. Fluctuation of the number of monthly rainfall was synchronous to the number of insect community collected by light traps (night flying insect) but tend to opposite to the number of insect collected by pitfall traps (ground insect).
TINGKAT KUALITAS KAYU EBONI (Diospyros celebica Bakh.) BERDASARKAN KOMPOSISI SERAT GELAP DAN TERANG Sunaryo Sunaryo
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3460

Abstract

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