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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
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Bentuk Sel Epidermis, Tipe dan Indeks Stomata 5 Genotipe Kedelai pada Tingkat Naungan Berbeda Sundari, Titik; Atmaja, Rahmat Priya
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.76 KB) | DOI: 10.14203/jbi.v7i1.3129

Abstract

ABSTRACTShape of Epidermal Cells, Type and Stomata Index of 5 Soybean Genotypes at Different Levelsof Shading. This study aimed to determine the shape of epidermal cells, type of stomata andthe stomatal index of five soybean genotypes at different levels of shading. Research conductedat the Screen House of Indonesian Legumes and Tuber Crops Research Institute in Marchuntil July 2007. Split plot design with repeated three times was used in this research. The mainplots were four levels of shading (0%, 25%, 50% and 75%) and subplot is the five soybeangenotypes (Pangrango, Tanggamus, Wilis, Lokon and IAC-100). Shading is used black screen.Plants fertilized with the 50 kg Urea + 100 kg SP36 75 + 75 kg KCl / ha. Fertilizing done atplanting time. The results showed that shade does not change shape of epidermal cell andstomata types of five soybean genotypes. Differences in stomatal index was not determinedby interaction of genotypes with levels of shading, but by the differences in shade andgenotype. Shading treatment increased stomatal index of soybean abaxial leaf surface. HighestSomatal index of soybean abaxial leaf surface is achieved by IAC 100 and the lowest is achievedby Wilis. Wilis is a genotype that can be adapted to the shaded environment, by maintainingleaf area, number of epidermial cells and stomata, stomatal index, and seed weight.Key words : soybean, shading, epidermis and stoma
Karakteristik Fisiologis Enzim Nitril Hidratase dan Amidase dalam Sel Corynebacteriurn sp. D5 Sulistinah, Nunik; Kaban, Joseva Sudiati; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.571 KB) | DOI: 10.14203/jbi.v3i4.3323

Abstract

ABSTRACTPhysiological Characteristics of Nitrile-Hydratase and Amidase From Corynebacteriumsp. D5. Nitrile hydratase (NH-ase) of Corynebacterium sp. D5 is inductive enzyme, butamidase is constitutive enzyme.The best inducer for Nitril hydratase is 2% (vlv) acetonitrille.Nitril hydratase and amidase enzymes showed to be capable of degrading low moleculeweight of aliphatic nitriles and amides. The optimum condition of NH-ase ofCorynebacteriurn sp. D5 were found out at pH 6,6 and 30°C while amidase at pH 7,2 & 50 Crespectively. The inhibitor of both enzymes seemed to be ~ gand H~*Key words : Nitrile hydratase, bioconversion, Corynebacterium sp. D5, amidase, acetonitrile,aliphatic nitrile
Pemurnian Parsial dan Karakterisasi Amilase dari Bakteri Laut Arthrobacter arilaitensis LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it’s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
Oksidasi Nitrit Oleh Bakteri Heterotrofik Pada Kondisi Aerobik Agustiyani, Dwi; Kayadoe, Ruly Marthina; Imamuddin, Hartati
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3164

Abstract

ABSTRACTNitrite Oxidation by Heterotrophic Bacteria Under Aerobic Condition. The nitrite transformingactivities of heterotrophic bacteria from isolates from agriculture soil, Lampung were studiedunder aerobic conditions. Among the 9 bacterial isolates tested, almost all are reported haveability to consume of nitrite, but none of the bacterial isolates formed significant nitrate in themedium. NOB H1 (Bacillus licheniformis), is denitrification-negative, consumed 16. 4 mg/L ofnitrites with the accumulation of 4.45 mg/L nitrates. While, NOB H8 (Pseudomonas sp.) isdenitrification-positive, consumed 49. 64 mg/L of nitrite with the accumulation of 3.34 mg/Lnitrates. Nitrite oxidations of both isolates NOB H1 and NOB H8 took place during stationeryphase to the dead phase. Growth pattern of both isolates NOB H1 and NOB H8 were sigmoidwith generation time of 1.69 and 2.19 hour, respectivelyKey words: heterotrophicbacteria; nitrite oxidation; denitrification
Keanekaragaman Jenis Gymnospermae di Pulau Wawoni, Sulawesi Tenggara Sunarti, Siti; Rugayah, Rugayah
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.22 KB) | DOI: 10.14203/jbi.v9i1.149

Abstract

berkembangbaik pada permukaan sisik atau daun, sering bermodifikasi membentuk kerucut atau di bagian akhir tangkaiyang pendek. Kelompok ini terdiri dari Conifers, Cycads, Ginkgo dan Gnetales. Ada sekitar 800 - 900 jenis dantelah dilaporkan bahwa Conifer mempunyai keanekaragaman jenis tertinggi (500 - 600 jenis) diikuti oleh Cycad(75 - 80 jenis). Inventarisasi dan eksplorasi telah dilakukan di Pulau Wawonii, sejak tahun 2003 sampai 2006.Enam jenis Gymnospermae yang diidentifikasi termasuk dalam 3 suku, yaitu Cycadaceae (Cycas rumphii), Gnetaceae(Gnetum gnemon dan G. cuspidatum), Podocarpaceae (Podocarpus neriifolius, Dacrydium nidulum dan Nageia wallichiana).Salah satu diantaranya merupakan rekaman baru untuk Sulawesi Tenggara.Kata kunci: Gymnospermae, keanekaragaman, Pulau Wawonii, Sulawesi
Daya Pacu Mikroba Pelarut Fosfat dan Penambat Nitrogen pada Tanaman Jagung Latupapua, H.J.D.; Suliasih, Suliasih
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.511 KB) | DOI: 10.14203/jbi.v3i2.3477

Abstract

ABSTRACTThe Stimulating Capacities of the Phosphate Solubilizing and the Nitrogen Fixing Microbes on the Maize Plant. The green house experiment has been carried out to study the effectiveness of the phosphate solubilizing microbes (PSM) and nitrogen fixing bacteria (NFB) on the growth of maize (Zea mays). The phosphate solubilizing microbes were Aspergillus sp. and Gigaspora margarita while the nitrogen fixing bacteria were Azospirillum sp. and Azotobacter sp. have been used in this experiment. The aim of the experiment was to find the suitable and effective inoculant on the maize and its expected the growth of the plant. The experiment used The Completely Randomize Design with three replicates. The treatments were uninoculated plant (control), the plants inoculated with single microbe of PSM, NFB and the combination of both. The results indicated that the phosphate solubilizing microbes (PSM) and the nitrogen fixing bacteria (NFB) which were either used in single or combination formula could stimulate the plant growth. The dual inoculation of Azotobacter sp. and Aspergillus sp. were found the higest yield of maize.Key words : The phosphate solubilizing microbes, the nitrogen fixing bacteria, Mycorrhiza vesicular arbuscular, Zea mays.
Potensi Serapan Karbondioksida (CO2) pada Beberapa Jenis Tumbuhan Lantai Hutan Dari Suku Araceae Di Taman Nasional Gunung Halimun- Salak, Jawa Barat Mansur, Muhammad
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.067 KB) | DOI: 10.14203/jbi.v8i2.3050

Abstract

Potential Absorption of Carbon Dioxide (CO2) in Some Plant species on The Forest Floor From Family of Araceae in The Gunung Halimun-Salak National Park, West Java. Study of carbon dioxide (CO2) absorption in some plant species on the forest floor from Family of Araceae conducted in the Gunung Halimun-Salak National Park, Resort Cidahu, West Java, on September 2011. The survey was conducted to inventory the species of Araceae that exist atthe study site. Thirty samples of the five species of Araceae has been chosen as the target of photosynthesis measurement. Six individuals from each species were measured as replicates,in which young and old leaves were measured from each individual. The results show that five species of Araceae are found in forest floor, namely; Schismatoglottis calyptrata, Schismatoglottis rupestris, Alocasia longiloba, Arisaema filiforme and Colocasia esculenta.Colocasia esculenta has a higher CO2 absorption rate (14, 288 ?mol/m2/s) than Arisaema filiforme (8,614 ?mol/m2/s), Alocasia longiloba (7,599 ?mol/m2/s), Schismatoglottis rupestris(7,078 mol/m2/s) and Schismatoglottis calyptrata (5,260 ?mol/m2/s). In general, old leaves of all species have higher chlorophyll contain and CO2 absorption rate than young leaves. Theoptimum photosynthetic rate in Schismatoglottis calyptrata occurred at 10:00 am (8,457 ?mol/m2/s) and the lowest at 16:00 pm (4,262 ?mol/m2/s).Keywords: CO2 absorption, Araceae, Gunung Halimun-Salak National Park.
Karakterisasi Enzim Nitril Hidratase dan Amidase dari Pseudomonas sp. BP3 dalam Biokonversi Adiponitril menjadi Asam Adipat Sunarko, Bambang; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 5, No 2 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.693 KB) | DOI: 10.14203/jbi.v5i2.3196

Abstract

ABSTRACTCharacterization of Nitrile Hydratase and Amidase of Pseudomonas sp BP3 in Bioconversionof Adiponitrile to Adipic Acid. Adipic acid is a commercially important compound, primarilyused as precursor for the production of nylon 6.6. It is also used for plasticizer, fibers, and foodadditive. Synthesis of adipic acid by chemical means requires large amount of energy andconcentrated acid. It also produces N2O as by product, which is very toxic and suspectedcauses depletion of the ozone layer. The purpose of this research was to study thebioconversion of adiponitrile by Pseudomonas sp. BP3 and to characterize the involved enzymesin the whole cell. Pseudomonas sp. BP3 was able to utilize adiponitrile as the sole source ofcarbon and nitrogen. It’s doubling time (td) and growth rate constant (?) during the growth inadiponitrile were 2 hours and 0.346/h, respectively. The optimum pH and temperature of nitrilehydratasewere pH 7.0 and 30°C, respectively, while those of amidase were pH 6 and 50°C.Vmax and Ks of nitrile hydratase were 8.3 nM/ml.min. and 55.56 mM, respectively, and ofamidase were 5,9 nM/ml.min and 50 mM. The rate of adiponitrile consumption was 0.245 mM/h and of adipic acid formation was 0.181 mM/h. The yield of bioconversion of adiponitrile andadipamide were about 50 % and 25%, respectively.Key words: Bioconversion, adiponitrile, adipic acid, Pseudomonas sp. BP3, nitrile hydratase,amidase
Pengembangan Sistem Deteksi Senyawa Sianogen dalam Ubi Kayu (Manihot esculenta Crantz) dengan Pendekatan Enzimatis Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.332

Abstract

Picrate paper test kit for the semi–quantitative determination of cyanogenic potential was developed in thisexperiment. The method is relatively simple, easy to use and might be applicable in the field by unskilled person.Paper test was attached on tubes containing sample (100 mg) in aquadest (0,5 mL) and then was immediatelycovered tightly and incubated overnight at room temperature. The colour of picrate paper test changed graduallytowards reddish brown, and its colour was compared with standart colour chart which included 0-800 ppm cyanidethat was also developed in this study. The reddish brown colour of paper test was correlated with cyanideconcentration on the sample. In order to obtain a more accurate detection of cyanogenic compound the paper testwas eluted with 5 mL water or aquadest and the absorbance was measured at 510 nm.Keywords: cyanogenic potential, picrate paper test, semi-quantitative method, simple method, cassava (Manihotesculenta Cranz)
Stuktur Komunitas Fauna Kumbang Sungut Panjang (Coleoptera: Cerambicidae) di Kawasan Taman Nasional Gunung Ciremai Noerdjito, Woro A.
JURNAL BIOLOGI INDONESIA Vol 4, No 5 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.53 KB) | DOI: 10.14203/jbi.v4i5.3220

Abstract

ABSTRACTCommunity Structure of Longicorn Beetles Fauna (Coleoptera: Cerambycidae)At Gunung Ciremai National Park. Longicorn beetles (Coleoptera: Cerambycidae)are useful for assessment of recovery of forest insect fauna, because mostly speciesfeed on woody plants and are expected to increase in number and diversity along withdevelopment of the forest. The longicorn beetles were collected at the Gunung CiremaiNational Park (GCNP) area, at Apuy track (Western part of GCNP) during three weeksin April 2006 and Linggarjati track (Eastern part of GCNP) also during three weeks inMay-June 2007. These beetles were collected by Malaise, Light, Artocarpus branchtraps (Bundled twigs of jack fruits tied onto a tree stem) and active collecting by sweeping,beating and handling methods. The specimens collected totally 184 individu, (38 species)which 12 species were noted found at Gunung Halimun Salak National Park (GHSNP)distributed at 1000-1300 meter above sea level.In the research we found Acalolepta rusticatrix, Epepeotes luscus and Pterolophiamelanura that known as a common species of Cerambycid or longhorn beetles inKalimantan and Java, were found in the research activities, caused these species foundat many places including at Bogor Botanical Garden. In GCNP, we did not find Nyctimeniusochraceovittata as indicator for relatively less disturbed forest in Kalimantan, otherwisewe find Nyctimenius varicornis mostly find in all study site at GCNP, that is possible asindicator for relatively less disturbed forest in Java. In GCNP, we find Gnoma longicollisas a cerambycid beetle for natural forest indicator. In the paper show the comparativediversity study between traps (collecting methods) and study site (habitat and altitude).Key word: Cerambycidae, Ciremai, struktur komunitas

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