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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
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VARIASI DAN KEKERABATAN GENETIK PADA DUA JENIS BARU BELIMBING (AVERRHOA LEUCOPETALA RUGAYAH ET SUNARTI SP NOV DAN A. DOLICHORPA RUGAYAH ET SUNARTI SP NOV., OXALIDACEAE) BERDASARKAN PROFIL RANDOM AMPLIFIED POLYMORPHIC DNA Yulita, Kusumadewi Sri
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3118

Abstract

ABSTRACTGenetic variation and relatedness of two new species of star fruit (Averrhoa leucopetalaRugayah et Sunarti sp nov and A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae)based on Random Amplified Polymorphic DNA. Two wild species of Averrhoa from Papuaand Gorontalo respectively has recently been described. These two species were previouslytreated as ?intermediate species? between A. carambola and A. blimbi on the basis ofmorphological characters. This present study aimed to assess genetic variation and geneticrelatedness of the two species compared to their relatives (A. carambola and A. blimbi) byusing Random Amplified Polymorphic DNA (RAPD). Five RAPD primers (OPA 9E, OPA 13,OPB 7, OPB 18 dan OPN 12) were used to amplify total DNA genom and produced 31 bands towhich 90.32% were polymorphic. These bands were ranging in size from 300-1700 bp. DNAfingeprints for each species was indicated by differences in RAPD profiles resulted fromamplification of five primers. Clustering analysis was performed based on RAPD profilesusing the UPGMA method. The genetic similarity range between 0.25-1.00 indicating widerange of genetic variations observed. Results also indicated that the two species weregenetically distant from A. carambola and A. blimbi, thus supported the recent morphologicaltreatment.Key words: Averrhoa, RAPD profiles, genetic variations.
DAMPAK KEGIATAN MANUSIA TERHADAP KERAGAMAN DAN POLA DISTRIBUSI KUMBANG SUNGUT PANJANG (COLEOPTERA: CERAMBYCIDAE) DI GUNUNG SALAK, JAWA BARAT Noerdjito, Woro Anggraitonongsih
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3066

Abstract

The impact of human activities on the Longhorn Bettles Diversity and Their Distribution Patterns in Mount Salak, West Java, had been conducted by observation and collecting efforts on various types of habitats which include undisturbed and disturbed forests. Longhornbeetles are one of the largest groups of wood borers and therefore typical forest dependent insects. The species diversity and distribution pattern of these beetles may vary with speciescomposition age of the trees, and stability of the forest, and therefore they are a useful bioindicator for assessing forest condition. The beetles were collected using ?Artocarpus branchtraps?, a bait trap. It is a bundle of about 5-6 freshly-cut branches of Artocarpus heterophyllus (jackfruit), about 80 cm long with many leaves, tied up and hung from a tree trunk or standingpoles at 1.5 m above the ground. The trap attracted longhorn beetles that need feeding for reproductive maturity. Ten traps were set at each study site (habitat). The attracted beetleswere collected every week by beating method, because in West Java forest area, beetles are usually gathered by the 6th day after trap setting. Light traps were also set up for 2 night, at each habitat for collecting nocturnal longhorn beetles. Totally 578 specimens were collected, consisted 38 species of longhorn beetles, dominated by small body size species less than 10mm. Out of these 38 species, some species were known distributed in certain habitat andelevation, other species have wider distribution, where as some species were endemic and new record for Java.Keywords: distribution, diversity, longhorn beetles, Mount Salak
DISTRIBUSI SPASIAL DAN TEMPORAL IKAN BONTI-BONTI (PARATHERINA STRIATA AURICH), ENDEMIK DI DANAU TOWUTI-SULAWESI SELATAN Nasution, Syahroma Husni
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3209

Abstract

ABSTRACTSpatial and Temporal Distribution of Bonti-bonti (Paratherina striata) an EndemicFish in Towuti Lake, South Sulawesi. Bonti-bonti (Paratherina striata) is an endemicfish species in Towuti and Mahalona Lake. This fish included into vulnerable species. Itshould be protected from decreasing of fish population due to increasing exploitationand habitat quality changes. The objective of this research to study on spatial and temporaldistribution of the fish, as a basic information for its conservation. Samples were collectedfrom May 2006 to April 2007 using experimental gillnet mesh size 0.625, 0.75, 1.0, and1.25 inches at five stations. Fish number and size captured during 15 hours. Waterquality parameters that analyzed were temperature, conductivity, pH, and dissolvedoxygen using water quality checker-Horiba, alkalinity used titration methode, whilewater level and rain fall obtained from PT. Inco. Distribution of fish abundance wasanalyzed with Mann-Whitney non-parametric test. Water quality parameters thatinfluencing fish abundance were analyzed by multivariate analysis. The results show thatthe distribution of bonti-bonti spreading widely starting from lakeside to middle of thelake. The highest abundance of the fish are in inlet with sand, gravel, and stone substrat.Fish size was obtained more various at the inlet station than other stasions and it predictedas main habitat. Water qualitiy parameters of were not factors influencing difference ofspatial distribution but it was influenced by behavior of habitat selection. The highesttemporal distribution of the fish abundance in November and December influenced bydissolved oxygen and high water level.Key words : Spatial and temporal distribution, Paratherina striata, endemic fish, abundance,Towuti Lake
KAJIAN GENETIKA UNTUK KONSERVASI BADAK SUMATERA (DICERORHINUS SUMATRENSIS GLOGER, 1841) Zein, Moch Syamsul Arifin; Fitriana, Yuli Sulistya; Kurniawan, Yuyun; Chaerani, Kurnia; Sirupang, Meriam
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3767

Abstract

ABSTRACTThe Sumatran rhinoceros is one of the most critically endangered species of large mammals due to habitat loss, fragmentation and illegal hunting so that the population of this species drastically decreased. At present, reproductive problems with a limited population are also a threat and require an appropriate solution. Therefore, data on molecular genetic information is very important as a basis for conservation management in maintaining long-term persistence of this species. Phylogenetic analysis based on sequences of CO1, 12SrRNA, and Cytochrome b gen from mitochondrial DNA genomes using neighbor-joining and genetic distance matrix calculations with the Kimura 2-parameter model (K2P) were implemented in pairwise distance calculations in the Mega (Molecular Evolutionary Genetics Analysis) program version 6.05. The study results show the genetic distance of Sumatran Rhinos from Sumatra and Kalimantan respectively 0.2 ± 0.00%, 0.8 ± 0.4%, and 0%. These results were reconfirmed that the Sumatran Rhino species in Sumatra and Kalimantan were taxonomy is no different. The study of genetic diversity based on D-loop of mitochondrial DNA contained 5 haplotypes, namely haplotypes 1 and 2 originating from the island of Sumatra and haplotypes 3, 4, and 5 originating from the island of Borneo. The genetic distance between individuals in this study ranged from 2.54 ± 1.4%, haplotype diversity (Hd) was 0.8 ± 0.172, nucleotide diversity (Pi) was 0.02269, Fu?s Fs value was 2.523, and Tajima?s test was 0.69497. The positive value (Fu?s Fs and Tajima?s test) indicated low genetic diversity and population expansion in the Sumatran rhino. In the study using 10 microsatellite loci, where the average number of allel/loci in Kalimantan (1.68) was higher than in Sumatra (1.22). Data from this study show that genetic variation between Sumatran rhinoceros from Sumatra and Kalimantan can be used as a basis for alternative that the populations of Sumatra and Borneo be considered as a single management unit.  Keywords: Sumateran  Rhinoceros, Mitochondrial DNA, Microsatellite
PENAPISAN DAN KARAKTERISASI RHIZOBAKTERIA SERTA UJI AKTIVITASNYA DALAM MENDUKUNG PERKECAMBAHAN DAN PERTUMBUHAN BENIH JAGUNG (ZEA MAYS L.) Agustiyani, Dwi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2890

Abstract

ABSTRACTPlant Growth Promoting Rhizobacteria (PGPR) are a group of bacteria known to influence plant growth by direct or indirect mechanisms. In search of efficient PGPR which potential as biofertilizers, a total of 26 isolates of rhizobacteria were isolated from various plant rhizosphere. These 26 isolates were screened through in vitro methods for PGPR properties like phosphate solubilization, siderophore, IAA and amonia production, and catalase activity. The result revealed that 9 isolates showed positive activities for phosphate solubilization 17 isolates for IAA production 18 isolates for siderophores 19 isolates for ammonia production and all of the isolates have catalase activities . Only four isolates (Az.KT.CSC, Az.D.8B, Az.D.8A and Az.Lo.10B) exhibited multiple plant growth promoting traits viz., phosphate solubilization, siderophore, IAA, amonia production, and catalase activity. Ten isolates that have different characters were further investigated for quantitative analysis of IAA production, HCN production and its effect on germination and seedling growth of Zea mays. The range of IAA production was 3,12? 134,27 ppm, among ten isolates, Az.D.8B isolate produced the highest IAA (134,27 ppm). Production of HCN was detected in eight isolates. Four isolates positively affected the germination of Zea mays seeds. Highest root elongation was recorded when seeds were treated with Az.Lo.5 isolate. Whereas, the highest chlorophyl content and plant high were recorded when seeds were treated with Az.B.8B isolate.Keywords: N-fixing bacteria, PGPR, IAA, siderophore, catalase
KARAKTERISTIK TIPE PAKAN KELELAWAR PEMAKAN BUAH DAN NEKTAR DI DAERAH PERKOTAAN: STUDI KASUS DI KEBUN RAYA BOGOR Soegiharto, Sri; Kartono, Agus P.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3176

Abstract

ABSTRACTFood TypeCharacteristic of the Fruit Bats at Urban Area: A Case Study in Bogor BotanicalGarden, West Jawa. Bats have important role on seed dispersal and or plant pollinator. Theidentification of flower and their pollens as the feed resource for bats was conducted in BogorBotanical Gargen. The resultsof this study showed that Eonycteris spelaea male has interestsin with personatus corola type flower, while the female in disk type. Furthermore the male ofMacroglossus sobrinus has interested in rotatus, tubulosus, and perferesence, corola types.Where as the female has interest in campanulatus type. The campanulatus and papilionaceustypes has a potential to be visitd by Cynopterus minutus male and female of C. sphinx;Urceolatus type has important for female of C. titthaheileus and C. brachyotis. The male ofMacroglossus sobrinus and female of Eonycteris spelaea has interests to visit the flower withsuboblate and prolate spheroidal pollen types; prolate pollen type has importance for themale of Eonycteris spelaea; oblate type for the male of C. minutus, C. brachyotis and C.titthaheileus; oblate spheroidal for the female of Rousettus amplexicaudatus and male of C.sphinx. The male of C. titthaheileus and female of Macroglossus sobrinus has interests ingigantic type (>200 ì m), while the female of C. sphinx, C. brachyotis and R. amplexicaudatuslike permagnae type (100-200 ì m).Key words: Fruit bats, pollen identification, urban areaKata kunci: Kelelawar buah, pollen, identifikasi, perkotaan
EFEKTIVITAS PROSES FITOREMEDIASI AIR IRIGASI TERCEMAR BAHAN ORGANIK MELALUI SISTEM BATCH CULTURE MENGGUNAKAN HIDROMAKROFITA LOKAL Retnaningdyah, Catur; Arisoesilaningsih, Endang
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3659

Abstract

ABSTRACTIrrigation water in Indonesia generally has polluted by organic matter due to human activities. The aim of study was to develop a package of phytoremediation technology that can effectively reduce organic pollutants in the irrigation water in order to ensure good quality of water irrigation to support organic farming. The study was conducted in greenhouses by planting some local emergent, submerged, floating-leaf hydromacrophytes and polyculture species using a batch culture system on a 30 L bucket with sand and gravel as a substrate. The study used a completely randomized design with the treatment were five types of hydromacrophytes (emergent such as Scirpus sp., Acorus calamus, Marsilea crenata; floating leaf macrophytes included Ipomoea aquatica, Azolla sp.; submerged such as Valisneria sp., Hydrilla verticilata; polyculture of the three hydromacrophytes, and controls without plant) which repeated three times at the same time. The effectiveness of the phytoremediation model for each treatment is determined by its ability to improve water quality as reflected by several physicochemical parameters of pH, DO, conductivity, turbidity, temperature, nitrate, orthophosphate, bicarbonate, BOD, TSS, TDS and TOM six days after incubation. Research results showed that batch culture for six days significantly reduced the organic matter content of irrigation water reflected from decreasing value of BOD, TOM, TSS, turbidity, nitrate, orthophosphate and bicarbonate levels. The six-day phytoremediation process in all treatments has not been able to increase the dissolved oxygen content in the water and has not been able to significantly reduce the conductivity and TDS. Hydromacrophytes submerged and polyculture were more effectively perform phytoremediation process compared with others.Keywords: Phytoremediation, local hydromacrophytes of emergent, submerged, and floating leaves, water pollution
IDENTIFICATION OF BIOACTIVE COMPOUND FROM MICROALGA BTM 11 AS HEPATITIS C VIRUS RNA HELICASE INHIBITOR Mustopa, Apon Zaenal; Umami, Rifqiyah Nur; Putri, Prabawati Hyunita; Susilaningsih, Dwi; Farida, Hilda
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2198

Abstract

ABSTRACTHepatitis C virus (HCV) is the major causative agent of chronic liver disease. Recently, the inhibition of NS3 RNA helicase/ATPase activity is being explored as the specifically targeted antiviral therapy (STAT) against HCV infection. This study was aimed to elucidate potential candidates for anti-HCV therapy derived from Indonesian indigenous microalgae. The microalga designated as BTM 11 was isolated and cultured. Methanol extract of BTM 11 was screened as the opponent of purified HCV NS3 RNA helicase enzyme through colorimetric ATPase assay. Screening of chemical compound and fractionation by using gel filtration chromatography with eluent of methanol : chloroform (1:99) were conducted for identification and isolation of the bioactive compounds. The third fraction of fractionated sample showed a relatively strong ATPase inhibitory effect (81.23 ± 2.25 %) compared to the negative control. Further analysis of third fraction using thin layer chromatography (TLC) with eluent of chloroform : methanol (9:2) gave two spots with the Rf value of 0.8 and 0.37, respectively. In addition, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 and 16.617 minutes which absorbed at 266 and 230 nm wavelenght, respectively. According to those analyses, this study suggests that bioactive compounds derived from BTM 11 were classified as the groups of flavonoids and feasible as potential candidates for anti-HCV therapy through the inhibitory effect of NS3 RNA helicase/ATPase activity. Keywords: Hepatitis C Virus, NS3 RNA helicase, ATPase, Microalga, Flavonoids 
EKSPLORASI KEANEKARAGAMAN AKTINOMISETES TANAH TERNATE SEBAGAI SUMBER ANTIBIOTIK Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 ? 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
PRODUKTIVITAS ILMIAH PENELITI INDONESIA PADA PENELITIAN KEANEKARAGAMAN HAYATI INDO-NESIA BERDASARKAN BASIS DATA SCOPUS 1990-2015 Amelia, Mia; Rahmaida, Rizka
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3398

Abstract

ABSTRACTThe rich of Indonesian biodiversity becomes an attraction for local and foreign researchers to conduct research in this country. One of scientific productivity indicators that mostly used is number of publication in scientific journal. The previous studies had never analyzed scientific productivity of Indonesian biodiversity researchers, both institutionally and individually. This paper analyzes 2,432 articles concerning Indonesian biodiversity research affiliated with institution from Indonesia (published in 1990-2015) from Scopus database, including 8,544 authors from 1,638 institutions. Data pre-processing resulted publication year, number of author, and author?s institution location. Then, they were analyzed using scientometrics analysis. Scientometrics analysis is a quantitative method related to measurement of the science communication. The result shows that internation-al collaborations produced most of scientific publications on Indonesian biodiversity. The number of scientific publication on Indonesian biodiversity was significantly increase and showed exponential growth trend. Indo-nesian author mostly wrote articles from Animalia kingdom. Multi and mega authors wrote the most of scien-tific publications on Indonesian biodiversity.Keywords : Indonesia, biodiversity, scientometrics, productivity, scientific publication, Scopus

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