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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 53   54   55   56   57 
PEMBENTUKAN DAN PENAMPILAN PISANG REJANG HIBRID TRIPLOID HASIL PERSILANGAN PISANG REJANG MIXOPLOID DENGAN PISANG REJANG DIPLOID Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Abstract

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids
PENGUJIAN 15 GENOTIPE KEDELAI PADA KONDISI INTENSITAS CAHAYA 50% DAN PENILAIAN KARAKTER TANAMAN BERDASARKAN FENOTIPNYA Susanto, Gatut Wahyu Anggoro; Sundari, Titik
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3151

Abstract

ABSTRACTThe Examination of 15 Soybean Genotypes at 50% Light Intensity and Evaluation of Crop?sPhenotypic Characters. Sunlight is one of the important plant growth requirements. In orderto understand morphological character changes in the crops due to different light intensity, 15genotypes of soybean consisted of Willis, D3578-3/3072-11, Seulawah, Aochi/W-62, Kaba,IAC 100/Brr-1, MLGG 0081, MLGG 0059, MLGG 0120, 9837/Kawi, D-6-185, IAC 100, MLGG0383-1, Pangrango, MLGG 0069 and MLGG 0122 were tested. The research was conducted inKendalpayak (grey Alluvial soil type, 450 above sea level, C3 climate type), Malang at dryseason in 2006. The research design was Randomize Complete Block under two differentenvironmental conditions, with three replications. The experiment was conducted under fulland 50 %light intensity. The results indicated that the reduction of light intensity as much as50 % resulted in some changes in phenotypic characters such as size and lifespan of the 15genotype being tested, included the increase of plant height, the longer distance betweennodes, the decrease in node number, the smaller size of stem diameter, the decrease on thenumber of leaves, the narrower of the leaf ?s width and the decrease in pod number. Lessenedseed weight, the low weight of 100 seeds, the lowering level of the leaf?s greenness, and theaccelerate age of flowering and harvesting. IAC 100, MLGG 0383-1 and IAC 100/BBR-1 producedhigh under 50% of light intensity.Keywords : Soybean (Glycine max (L) Merrill), light intensity, phenotypic
PENCIRIAN PRODUKSI AMILASE OLEH SACCAROMYCES CEREVISIAE W303A REKOMBINAN Thontowi, Ahmad; Puspaningsih, Ni Nyoman Tri; Hadi, Sofjan; Purkan, Purkan; Ni'mahtuzahroh, Ni'mahtuzahroh; Irawan, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3464

Abstract

ABSTRACTCharacterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively.Key words: Characterization, amylase, S. cerevisiae W303a
KARAKTERISTIK SUARA RHACOPHORUS EDENTULUS MUELLER, 1894 ASAL PEGUNUNGAN MEKONGGA, SULAWESI TENGGARA (ANURA: RHACOPHORIDAE) Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2152

Abstract

Vocalizations Rhacophorus edentulus Mueller, 1894 have never been described before. The advertisement calls of four individual males of R. edentulus which originated from Mekongga Mountains, Southeast Sulawesi were recorded in December 7-8, 2010 at air temperatures of 20.5°C.  Location of sound recording for the four individual males were performed at two different places, call of one individual was recorded when it was calling in a plastic bag in the base camp, while calls of the other three individuals were recorded in their habitat in the forest. Adobe Audition 3.0 software was used to visualize sound waves.  Calls of R. edentulus have two types, i.e. pulses and pure tone.  Sound wave of pulses has two types, namely pulse type 1 and pulse type 2; whereas sound wave of pure tone also has two types, namely pure tone type 1 and pure tone type 2.  Based on the dominant frequency and the fundamental frequency of pure tones waves and pulse waves, the natural noise in the forest habitats affects the dynamics of the frequency of calls that were released by males R. edentulus; frequency sound with low noise is more dynamic than the frequency of the sound with high noise level. Key words: vocalization, Rhacophorus edentulus, Mekongga Mountains, Southeast Sulawesi. 
PELESTARIAN LINGKUNGAN BERBASIS KEPERCAYAAN LOKAL DAN UPACARA TRADISI: STUDI KASUS MASYARAKAT DI SEKITAR GUNUNG SALAK Royyani, Mohammad Fathi; Walujo, Eko Baroto
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3067

Abstract

Mount Salak in West Java has important position as water catcment area of water, rain forest, and bank of biodiversity. To protect and conserve this area, Indonesian goverment through the Forest Department develop has Mount Salak to be National Park. Local people who live surrounding Mount Salak have local wisdoms about the benefit of plant and local perspective to manage biodiversity. For local people, Mount Salak is not only to their daily needs but also to save their history and legend about their ancestor. To collect data, we conducted indepth interview with local peple, observed and involved with their ritual local people of Mount Salak have ritual tradition and local belief about Mount Salak, that keep Mount Salak area protected and conserved sustainability.Keywords: Biodiversity, Gunung Salak, Kepercayaan Lokal, Konservasi, Upacara tradisi,
KELEMAHAN GEN 12S RRNA UNTUK MEMPELAJARI STRUKTUR POPULASI GENUS AETHALOPS (CHIROPTERA: PTEROPODIDAE) DI INDONESIA: TANGGAPAN TULISAN MAHARADATUNKAMSI & M. SYAMSUL ARIFIN ZEIN PADA JURNAL BIOLOGI INDONESIA 4 (2): 75-86. Sutrisno, Hari
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3210

Abstract

TULISAN PENDEKKelemahan Gen 12S rRNA Untuk Mempelajari Struktur Populasi GenusAethalops (Chiroptera: Pteropodidae) di Indonesia: Tanggapan TulisanMaharadatunkamsi & M. Syamsul Arifin Zein pada Jurnal BiologiIndonesia 4 (2): 75-86.
PENGELOLAAN KANDUNGAN BAHAN ORGANIK PADA LIMBAH CAIR LABORATORIUM PROLINGMSP-IPB DENGAN BERBAGAI KOMBINASI AGEN BIOREMEDIASI Pratiwi, Niken Tunjung Murti; Hariyadi, Sigid; Ayu, Inna Puspa; Apriadi, Tri; Iswantari, Aliati; Wulandari, Dwi Yuni
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3768

Abstract

ABSTRACT Wastewater from laboratory glassware washing activities contains organic materials. Decomposing bacteria could increase the biomass by utilizing organic matter as a source of carbon. This research was aimed to describe the influence of commercial probiotic application in reducing organic matter content of Proling laboratory wastewater. The research was conducted from February to July 2016 in Aquatic Microbiology Laboratory. The experiment consists of several treatments, those were wastewater (L), wastewater+molase (LM), and wastewater+molase+bacteria from commercial probiotic (LMB). Major parameters observed were COD and bacteria abundance. The results showed that there was significant response difference between molasses and without molasses treatment. Based on COD, the most influenced treatment in decreasing organic matter were LM and LMB. These treatments could decrease COD up to 97% and 91%. Furthermore, the abundance of bacteria in molasses treatment was higher than without molasses. Based on this study, the most effective treatment in reducing organic matter was LMB.  Keywords: bacteria, laboratory wastewater, molasses, organic matter
EFFECT OF NITROGEN ADDITION ON THE ?-AMYLASE PRODUCTION BY ASPERGILLUS NIGER, RHIZOPUS OLIGOSPORUS AND NEUROSPORA CRASSA IN MEDIA CONTAINED SARGASSUM AND RICE SEED ON SOLID STATE FERMENTATION Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2891

Abstract

ABSTRACTAmylase is one of the hydrolytic enzymes which is commercially important and represent about 25?33% of the world enzyme market. Amylase production is affected by substrate for enzyme productions include particle size, initial moisture content and nutrient addition. The present study describe the effect of N-species addition on alpha amylase production by Aspergillus niger Km1, Rhizopus oligosporus Km2 and Neurospora crassa Km3 in medium contained sargasum and rice seed on solid state fermentation. Various ration of media composition contained dried sargassum and rice seed were studied. The effect of particle size of sargassum, initial water content on ?-amylase production were evaluated. The best media composition was then augmented with N-species include sodium nitrate, yeast extract, and peptone on solid state fermentation. Best media composition was 60:40 (w/w) of sargassum and rice seed respectively, with initial moisture content was 60%. Increase 5 times of amylase activity was obtained when sodium nitrate (0,5% w/w) was added to production medium. N-species significantly affect Alpha amylase production on sargassum and rice seed with maximum alpha amylase production was 36,66 unit by Rhizopus oligosporus.The economic value of Sargassum can be increased through alpha amilase production.Keywords: Amylase, Nitrogen, solid state fermentation, Rhizopus oligosporus, Neurospora crassa
IDENTIFIKASI PAPASAN (COCCINIA GRANDIS (L.) VOIGT) DI TIGA POPULASI DI YOGYAKARTA Rindyastuti, Ridesti; Daryono, Budi Setiadi
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3177

Abstract

ABSTRACTSpecies Identification of Scarlet gourd (Coccinia grandis (L.) Voigt) in Three Population inYogyakarta. Papasan is a dioecious plant belongs to the family Cucurbitaceae. This plant iscommonly used as vegetable, anti diabetic, anti bacterial, and anti diarrhea. In Daerah IstimewaYogyakarta, there were two varians of Papasan (Papasan I and II), found in three population(Ngebel, Berbah and Gajah Wong Riverbank). They differ in phenotype, especially in shapeand taste of fruit. Genotype observation using squash method on the root tips with modificationin the duration of maceration were used in this research indicated that cells devided of PapasanI at about 8-11.30 a.m, while Papasan II at about 08.30-09.30 a.m, 11 a.m-00.30 p.m and 2-2.30p.m. The chromosome number of both Papasan is 2n=24, contains of 22 autosomes and 2 sexchromosomes. The karyotype formulas of Papasan I and II were 2n=24=20m+2sm+XX(m).Based on the statistic test, significant difference on chromosomes character between PapasanI and II was only in short arm of autosome pair number 5. The difference R value betweenPapasan I and II was smaller than 0.25. It revealed that the both of Papasan is closely relatedand belongs to the same species of Coccinia grandis L. Character differences between bothof Papasan only revealed physiology adaptation.Keywords: Coccinia grandis L., Papasan, Chromosome, Karyotype.Kata kunci: Coccinia grandis L., Papasan., Cromosom, Kariotype.
SELEKSI KONSORSIUM MIKROORGANISME UNTUK PEMBENAH LUMPUR SIDOARJO SEBAGAI MEDIA TANAM Pikoli, Megga Ratnasari; Setyawati, Adi Puji; Larasati, Tri Retno Dyah; Mulyana, Nana
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3660

Abstract

ABSTRACTThe mud that erupted in Sidoarjo, East Java, has been known from several studies can be used as plant growth media. However, its ability has limitations, particularly due to the very high heavy metal content and phenol will gradually poison the plants. Therefore, a study has been conducted in order to obtain a consortium of microorganisms which have ability to support plants growth and remediate the mud so that further can be used as a growth media. The selected microorganisms consist of Azotobacter sp. KDB2, Bacillus sp. KLBN1, Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 and Trichoderma sp. PJF7F7, which were combined in five consortia, namely K1, K2, K3, K4, and K5. The selection was performed on the ability to produce indole acetic acid, dissolve phosphate, decrease phenol concentration, and decrease the concentration of Pb and Cd ion metals. The results showed that the five consortia have capacity in the five parameters examined. The ability to remove Pb by microbial consortia which consist of Azotobacter, Bacillus, Pseudomonas, and Trichoderma is a new information of the role of microorganism on heavy metal removal. The existence of positive and negative interactions within each consortium lead to differences in the kind of superior consortium generated from each parameter. The consortium K4 showed advantages in 3 parameters, that were in the indole acetic acid production and the Pb and Cd decreases, while the other consortia showed the best capacity only on one of the parameter examined. Therefore, the selected consortium recommended for fixing the Sidoarjo mud is K4, which consists of Azotobacter sp., Bacillus sp. BMC4, Pseudomonas sp. BMC6, Trichoderma sp. PJF6 dan Trichoderma sp. PJF7.Keywords: biofertilizer, bioremediation, Azotobacter, Bacillus, Pseudomonas, Trichoderma

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