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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 68   69   70   71   72 
PENGEMBANGAN IDENTITAS SPESIFIK BERBASIS MARKA SSR PADA 29 VARIETAS KEDELAI LOKAL INDONESIA Lestari, Puji; Risliawati, Andari; Utami, Dwinita Wikan; Hidayatun, Nurul; Santoso, Tri Joko; Chaerani, Chaerani
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2888

Abstract

ABSTRACTSoybean is an important legume crop in Indonesia and local varieties from different regions are diversed. Accurate identification of local varieties is needed to determine the genetic identity in order to protect them to indicate the geographical origin and registration purpose. This study aimed to analyze DNA fingerprints of local varieties of soybean in Indonesia to develop a specific identity using SSR markers along with the marker set. A total of 29 local varieties/accessions was analyzed using 9 selected SSR markers with semi automated genetic analyzer on the basis of capillary electrophoresis. Total markers successfully detected 50 alleles in all varieties, with the average polymorphism information content (PIC) of each locus (0.579) and the genetic diversity index (0.629) were quite high as a reflection of diversity of the local varieties. Based on the analysis of the genetic diversity index, PIC, rare alleles, dominant allele frequency, analysis of genetic distance, 6 SSRs (Satt009, Satt038, Satt177, Satt242, Satt308 and Satt114) were selected as a set of markers for variety identification. Although three varieties can not be distinguished because of their close genetic relationship, but the digital coding indicates that local varieties of soybeans have possessed specific identities (ID) representing variation of SSR alleles. The selected SSR loci with additional identifier can be used to develop ID of Indonesian local soybean in the germplasm collection. Overall, the marker set is a powerful tool to complement morphological markers for the protection of local soybean varieties that contribute in accelerating local varieties improvement in the future agriculture.Keywords: specific identity, SSR, Indonesian local soybean variety, DNA fingerprint.
TOKSISITAS ISOLAT BACILLUS THURINGIENSIS YANG MENGANDUNG GEN CRY 1A TERHADAP HAMA PENGGEREK BATANG JAGUNG, OSTRINIA FURNACALIS GUENEE Bahagawati, Bahagawati; Rizjaani, Habib; Sibuea, Agustina K.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3174

Abstract

ABSTRACTCry genes isolated from Bacillus thuringiensis produce crystal proteins that exhibit a highinsecticidal activity against several plant pests. The objectives of this experiment were todetect the presence of cry1A sequences from several local Bacillus thuringiensis isolatesmultiplied by Lep1A-Lep1B and Lep2A-Lep2B primers using PCR technique and to determinetheir toxicity against Ostrinia furnacalis, maize stemborer. From 59 tested isolates, 6 of themgave PCR products, two DNA bands, first was 490 bp, the expected size of Lep1A-Lep1Bprimers, and second was 986 bp, the expected size of Lep2A-Lep2B primers. These isolateswere Jtg 2151, 243, Cib 551, Lam 752, Lam 762 and C 522. All of tested isolates showedpotentially high toxicity against maize stemborer.Key words: B. thuringiensis, cry1A, Ostrinia furnacalis, PCR.Kata kunci: B. thuringiensis, cry1A, Ostrinia furnacalis, PCR.
PUPAL ACOUSTIC BEHAVIOUR OF TROIDES HELENA HELENA (LINNAEUS, 1758) (LEPIDOPTERA: PAPILIONIDAE) FROM WEST JAVA, INDONESIA Kurniati, Hellen; Peggie, Djunijanti; Sulistyadi, Eko
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3657

Abstract

ABSTRACTTroides helena helena (Linnaeus, 1758) can be found in Java and Bali. The larval and adult stages of this butterfly do not produce any sound, but the pupal stage produces sound in response to a touching stimulus. Sony PCM-M10 recorder and Pettersson M500 USB Ultrasound Microphone were used to record the sound. Adobe Audition 3.0 was used to visualize the oscillogram and audiospectrogram of the sound. Beyond our expectations, the pupae produce ultra sound hisses which are composed of many impulses. A one-day-old pupa has a frequency range between 0.2-40 kHz; however of 2-days to 18-days-old has a frequency range between 0.2-150 kHz. The sound wave consist of two sound elements, i.e. the audible sound element (0.2-20 kHz) and inaudible sound element (>20-150 kHz) and the bandwidth is between 200 Hz to around 150 kHz, with the strongest spectrum energy at around 2.6 kHz, 4 kHz, 6 kHz, 9 kHz and 23 kHz and some strong spectrum energy is also visible at around 40 kHz.Key words: acoustic, behaviour, butterfly, pupa, Troides helena helena
PENGARUH ANTIOKSIDAN EKSOPOLISAKARIDA DARI TIGA GALUR BAKTERI ASAM LAKTAT PADA SEL DARAH DOMBA TERINDUKSI TERT-BUTIL HIDROPEROKSIDA (T-BHP) Afiati, Fifi; Widad, Nina Ainul; ., Kusmiati
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2196

Abstract

 ABSTRACTExopolysaccharides (EPS) are water soluble polymers synthesized by three strains of lactic acid bacteria (LAB) namely EPS-S1, EPS-S2 and EPS-S7. Some exopolysaccharides (EPSs) produced by lactic acid bacteria (LAB) present potential health-beneficial properties, such as immune stimulation and antioxidative activities. Antioxidants function in several ways including preventing the formation of radicals, scavenging free radicals, formation of hydrogen peroxide and lipid peroxides. The Objectives of this research were to evaluate the antioxidant activities of exopolysaccharides (EPS) extracts from lactic acid bacteria (LAB). The extracts were investigated by using in vitro assays induced tersier-Butilhidroperoksida (t-BHP) 6 µg.ml-1 red blood cell (RBC) for their effects on lipid peroxidation malondealdehyde (MDA) level and  activities of some antioxidant enzymes which includes catalase (CAT) and superoxide dismutase (SOD). Six groups consisted of 3 replicated per group; (I) normal control, RBC (II) negative control, RBC+ t-BHP (III) positive control, RBC+t-BHP+vitamin-C, and (IV-VI) samples, RBC+t-BHP+EPS (S1, S2 and S7) extracts. The results showed that exopolysaccharides (EPS) of three strains of lactic acid bacteria (LAB) increased catalase (CAT) and superoxide dismutase (SOD) enzyme activity and decreased malondealdehyde (MDA) content, exopolysaccharides (EPS)-S2 most potential to increase the activity of catalase (CAT; 77.46%) and superoxide dismutase (SOD; 33.87%) enzyme and inhibit the increase malondealdehyde (MDA; 32.19%) levels. Keywords: exopolysaccharide, lactic acid bacteria, malondealdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) 
ZINGIBERACEAE OF THE TERNATE ISLAND: ALMOST A HUNDREAD YEARS AFTER BEGUIN’S COLLECTION Andriyani, Marlina
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3139

Abstract

ABSTRAKZingiberacea Pulau Ternate: Hampir Seratus Tahun Koleksi Beguin. Ditemukan sepuluh jenis Zingiberaceae yang mewakili lima marga (Alpinia, Etlingera, Hornstedtia, Globba, Boesenbergia) di Pulau Ternate. Alpinia novae-pommeraniae K. Schum. dan A. pubiflora (Benth.) K. Schum. merupakan catatan baru untuk Maluku. Pengkoleksian kembali Alpinia regia dari lokasi tipe memberikan tambahan informasi baru di mana spesimen tipe (Beguin1234 di herbarium L) tidak lengkap. Kata kunci: jahe-jahean, Maluku, Pulau Ternate, Beguin.
KARAKTERISTIK MORFOLOGI DAN HABITAT IKAN BREK (BARBONYMUS BALLEROIDES VAL. 1842) DI SUNGAI SERAYU JAWA TENGAH Hayono, Hayono; Rahadjo, M F; Affandi, Ridwan; Mulyadi, Mulyadi
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3396

Abstract

ABSTRACTBrek is a consumption fish belong to barbs group that widely distributed, one of them is in Serayu river. The aims of this study was to reveal the characteristics of meristic, morphometric, and habitat of the species. The study was conducted on the Serayu river especially in Banjarnegara, Central Java Province. Sampling was conducted on May 2012 to June 2013. The area of study was divided into three zones and the zones was categorized based on Reservoirs at Mrica area these are upper zone, reservoir zone, and below the reservoir zone. Fishing gear used are cast net, elektorfishing, and gill nets. Samples of fish were caught preserved in 4-10% formalin solution and then observed in the laboratory. Morphometric characters observed were 24 characters. The results were standardized to standard length, then analyzed with discriminant methods. The structure of the scales and number of ridges on the back of the last dorsal fin spines also observed. The results meristic characters are 4.8 dorsal fin, 3.5 anal fin, 1.14-16 pectoral fin, and predorsal 10-13 scales, also there were no spots on the caudal peduncle. The results of discriminant analysis showed known five characters distinguishing three species near 100%. The habitats have rocky bottom, rapid current, and divide into two groups.Keywords: barb fish, meristic, morphometric, Serayu river
KOMPOSISI FLORISTIK DAN STRUKTUR HUTAN DI PULAU NATUNA BESAR, KEPULAUN NATUNA Mirmanto, Edi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2100

Abstract

Ecological study has been conducted in the forest area of Natuna Island to reveal the structure and composition oftree species. In the 10 plots of 20 x 40 m each, there were 141 species of trees, with a density of 504 trees / ha and12.35 m2/ha of basal area, which was distributed in three forest types. Myrtaceae was the most common family,followed by Euphorbiaceae and Dipterocarpaceae. On the other hand Arthrophyllum javanicum recorded as themost common species together with Syzygium aemum and Ilex pleiobrachiata. The existence of each of the common speciesvaries in each forest type that shows there was a correlation between the condition of the habitat and the presence ofspecies.Keywords: floristic composition, structure, the Natuna Besar, Riau
ARSITEKTUR RIGI-RIGI DERMATOGRAF DARI MARGA HYLOBATES DAN SYMPHALANGUS (FAMILI HYLOBATIDAE) DI INDONESIA Maryanto, Ibnu; Kartono, Agus Priyono; Putro, Gembong IR Sunarto
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3102

Abstract

ABSTRACTThe analysis of palm and sole arschitexture dermatographic traits have been done for two sub genera of Hylobates and Symphalangus in Indonesia. A dermatographical study was conducted to specimens from Indonesia Archipelago on genera Hylobates and Symphalangus, which are deposited in Museum Zoologicum Bogoriense-LIPI. A total of 101 specimens of Hylobates (78 specimens) and Symphalangus (23 specimens) were examined on palm and sole dermatographic traits. The discriminant canonical analyses showed that the Indonesian gibbon has two differences in function space of dermatograph types. By plotting these data, the percentage of the two groups that correctly classified was calculated to be 90.63%. Only nine specimens of Hylobates and Symphalangus were incorrectly classified, seven (9.5%) specimens Hylobates were classified as Symphalangus, and two specimens Symphalangus (9.1%) were classified as Hylobates. The majority of loops type dermatograph in this study indicated that the traits direction to ulnar, radial, and proximal between Hylobates and Symphalangus are significantly different (X2 >0.05, df=3; X2 = 13.58, 10.03 and 7.91 respectively).Keywords: Architecture Dermatographic, Hylobates, Symphalangus
VARIASI GENETIKA KELELAWAR GENUS AETHALOPS (CHIROPTERA: PTEROPODIDAE) DI INDONESIA: TINJAUAN DARI GEN 12SRRNA PADA DNA MITOKONDRIA Maharadatunkamsi, Maharadatunkamsi; Zein, M. Syamsul Arifin
JURNAL BIOLOGI INDONESIA Vol 4, No 2 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i2.3266

Abstract

ABSTRACTGenetic Variation of Bat in the Genus Aethalops (Chiroptera: Pteropodidae) fromIndonesia: Analysis of 12SrRNA Gene of Mitochondria1 DNA. We report a geneticvariation on 12SrRNA gene to investigate haplotype and nucleotide diversity, as well aspopulation structure of bats in the genus Aethalops. Samples consisted of 21 individualsfrom 7 populations of 5 islands (Sumatra, Jawa, Kalimantan, Bali and Lombok) coveringall islands distribution of this genus in Indonesia. Total DNA was extracted from livertissues. Fragment 12SrRNA gene region of the mitochondria1 DNA were amplified byPolymerase Chain Reaction usingprimers of L 1091 and H 1478. Nucleotide sequenceswere determined to investigate genetic variation of this genus. Results indicated thatfrom 294 bp nucleotide sequences, 33 polymorphic sites with 13 haplotypes were foundamong 7 populations.Key Words: Variation, Bat, Aethalops , Chiroptera, Indonesia, 12SrRNAmitochondrial.
PERTUMBUHAN DAN PRODUKSI JAGUNG PULUT LOKAL SULAWESI SELATAN YANG DITANAM DI POLIBAG PADA BERBAGAI KOMBINASI PERLAKUAN PUPUK ORGANIK Juhaeti, Titi; Hidayati, N; Rahmansyah, M
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.168

Abstract

Research has been carried out through the utilization of organic fertilizer to improve the productivity of local maizecultivar originated from South Sulawesi. The study was conducted at the research station of Research Center for Biology,Cibinong Science Center. Corn seed were planted in polybag containing mixture of soil and compost, 6 and 2kg, respectively. The research were carried out by Randomized Complete Block Design arranged in factorial experimentwith four replications. The first factors are three types of local maize namely A: rice corn (pulut beras, BataraKoasa), B: waxy corn (pulut biji, Batara Kamu), C: pulut hibrida (hybrid cultivar). The second factors are 14 combinationtreatment as a mixture of LIPI organic fertilizer (Beyonic-StarTmik, Bio121, EM-121 and MegaRhizo) andanorganic NPK fertilizer. The variables observed were plant growth and production. The results showed that thehybrid cultivar had higher total corn yield productivity compared to the local one, although hybrid cultivar hassmaller plant biomass. Waxy corn (B) accession showed the weight, length and diameter of cob, and also the weightof 100 grains larger than rice corn (A). Fertilization treatments significantly affect the corn-cob productivity which isincludes the weight, length, and diameter units. Fertilization treatments such as EM-121 + (½ dose of NPK), BIO-121 + (½ dose of NPK), and Beyonic + (¼ dose of NPK) showed a good effect on plant, and it was not significantlydifferent with 100% NPK (full doses of NPK). The three corn cultivar showed different responses to fertilizationtreatments.Keywords: local corn, biofertilizer, growth, production

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