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All Journal AGRIVITA, Journal of Agricultural Science
Ullah, Rehan
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Agriculture, Biological Sciences & Forestry

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Optimization of Somatic Embryogenesis Formation in Hoya (Hoya carnosa (L.) R. Br.) with Different Hormone Combinations Restanto, Didik Pudji; Prayoga, Mohammad Candra; Jaenuri, Ahmad; Avivi, Sholeh; Soeparjono, Sigit; Selopa, Tanaya Asmara Citra; Ullah, Rehan; Alfarisy, Fariz Kustiawan
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026): IN PRESS
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.4844

Abstract

Hoya carnosa (L.) R. Br. propagation is conventionally less efficient, and the amount produced is low. This study aims to determine the influence of hormone type and concentration on changes in cell structure and the phase of somatic embryogenesis of the Hoya plant in vitro. The study was conducted in two stages, namely the first stage of callus induction with a combination of BAP (0.4 mg/l, 0.5 mg/l, 0.6 mg/l) and 2,4-D (5 mg/l, 6 mg/l, 7 mg/l) and the second stage of somatic embryogenesis with a combination of BAP hormones (0.5 mg/l, 1.0 mg/l, 1.5 mg/l) and IBA (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The data were analyzed using ANOVA and further analyzed by using DMRT. Based on the results, the combination of BAP 0.4 mg/l and 2,4-D 7 mg/l hormones showed the best growth with the parameters of the appearance of callus at 23 days, callus weight 2.92 g, and embryogenic calluses formed with crumb texture and greenish-yellow color. The combination of BAP 1 mg/l and IBA 0.75 mg/l resulted in the frequency of budding emergence of 23.3 days and the number of tendrils 20.7. This treatment was able to produce 5 roots and 2 shoots.
Co-Authors Alfarisy, Fariz Kustiawan Jaenuri, Ahmad Prayoga, Mohammad Candra Restanto, Didik Pudji Selopa, Tanaya Asmara Citra Sholeh Avivi Sigit Soeparjono