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Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI) Sat Rahayuwati; Sri Hendrastuti Hidayat; Purnama Hidayat
Jurnal Entomologi Indonesia Vol 13 No 3 (2016): November
Publisher : Perhimpunan Entomologi Indonesia

Bemisia tabaci (Gennadius) is an important horticulture pest and is know to be a vector of Geminivirus. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by Geminivirus, know to be transmitted by B. tabaci. It became an important disease in pepper and could cause 100% yield loss. Currently there is limited information concerning the genetic of B. tabaci associated with Geminivirus in Indonesia. It is therefore important to investigate the genetic identity of B. tabaci across different population. The aim of this research was to investigate the genetic identity of B. tabaci that were collected from pepper yellow leaf curl endemic area in the western part of Indonesia. Research activities include 1) sample collection of B. tabaci from pepper yellow leaf curl endemic area in west Indonesia; 2) single extraction of B. tabaci total DNA; 3) amplification of mitochondrial Cytochrome Oxidase I gene (mtCOI) fragmen with polymerase chain reaction (PCR); 4) mtCOI sekuencing 5) phylogenetic analysis of mtCOI fragmens from west Indonesian PYLCIV compared with some B. tabaci mtCOI deposited in National Center for Biotechnology Information (NCBI). Based on mtCOI fragment analysis, B. tabaci from PYLCIV endemic area were in one group belong to Asia I clusters. Genetic identity of B. tabaci from endemic area in Western Part of Indonesia were similar with polyatomic cladogram.

Variasi morfologi puparium Bemisa tabaci (Gennadius) (Hemiptera: Aleyrodidae) pada berbagai inang dan ketinggian tempat dari daerah endemik penyakit kuning cabai di Wilayah Sundaland Sat Rahayuwati; Purnama Hidayat; Sri Hendrastuti Hidayat
Jurnal Entomologi Indonesia Vol 17 No 2 (2020): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.17.2.61

Bemisia tabaci (Gennadius) is one of polyphagous whitefly that has been known as gemini virus vector. The identification of B. tabaci is carried out based on pupal case or puparium of red eye fouth stage. The morphological variation of B. tabaci puparium leads to the difficulties on species identification. This research was aimed to study the morphological variations of B. tabaci puparium that has been found in various host plants at various altitude. Samples of B. tabaci puparium were obtained from Sundaland endemic area of pepper yellow curl disease virus endemic areas including West Sumatra, West Java, Central Java, East Java, Bali, South Kalimantan. Slides-mounted were made from puparium and then identification was carried out to species. Observations have been conducted on B. tabaci puparial size, puparial shape, number of elongated dorsal setae, and caudal setae size. Canonical correlation analysis was applied to determine the factors that affected the puparium morphological variation. Based on the results, there were four variations of puparium: oval, oval with 1–2 indentations, oval with more than 3 indentations, and sea shell shapes. The variation observed on dorsal setae number, puparial shape, and size of B. tabaci were supposed due to the induction of trichomes on the leaves surface. Puparium variations were affected by host plants instead of altitude.

Ekotoksikologi Pestisida Decis 25 E, Curacron 500 EC, Score 150 EC dan Bactospein WP terhadap Lele (Clarias batrachus) dan Nila (Oreochromis niloticus) Rahayuwati, Sat
Agroprimatech Vol. 6 No. 2 (2022): Agroprimatech
Publisher : Prodi Agroteknologi Fakultas Agro Teknologi Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Aplikasi pestisida pertanian yang berlebihan dan tidak tepat dapat terbawa oleh air hujan hingga ke perairan. Decis 25E yang tergolong insektisida Pyrethroid, Curacron 500 EC termasuk insektisida golongan Organofostat, Score 150 EC fungisida sistemik dan insektisida biologi Bactospein WP diuji tingkat toksisitasnya terhadap Lele (Clarias batrachus) dan Nila (Oreochromis niloticus) pada konsentrasi 0,1 ppm; 1 ppm; 10 ppm; dan 100 ppm dengan pelarut air. Decis 25E Curacron 500 EC memiliki tingkat toksisitas tinggi terhadap Lele dan Nila yang menimbulkan pendarahan pada ikan uji dari konsentrasi 0,1 hingga 100 ppm dalam waktu lebih dari 1 jam. Sehingga adanya runoff Decis 25E dan Curacron 500 EC dengan konsentrasi 0,1 ppm di perairan dapat mengakibatkan kematian pada ikan. Fungisida Score 250 EC dan insektisida biologi Bactospeine WP cukup aman terhadap ikan lele dan nila pada konsentrasi 0,1 ppm.

Deteksi Keberadaan Nematoda Parasit Elaeolenchus parthenonema Pada Serangga Penyerbuk Kelapa Sawit Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) dari Deli Serdang dan Simalungun Sumatera Utara Ompusunggu, Hana Herawati; Purba, Josua Aldon; Tamba, Yudi Jhosua; Rahayuwati, Sat; Pradana, Mahardika Gama; Prasetyo, Agus Eko
JURNAL AGROPLASMA Vol 12, No 2 (2025): Vol 12, No 2 (2025): (JURNAL AGROPLASMA VOLUME 12 NO 2 TAHUN 2025)
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v12i2.7599

In Indonesia, numerous reports indicate a decline in the population of the oil palm pollinating insect (SPKS) Elaeidobius kamerunicus (Coleoptera: Curculionidae). The parasitic nematode Elaeolenchus parthenonema has not yet been investigated as a potential agent contributing to this SPKS population decline. This research aims are to determine the presence of parasitic nematodes in SPKS populations from Simalungun Regency and Deli Serdang Regency, North Sumatra Province, and to understand the life cycle of these parasitic nematodes. The parasitic nematode E. parthenonema was found, with a low infestation severity per individual SPKS: 1.9 nematodes from Simalungun and 4.94 nematodes from Deli Serdang. Juvenile nematodes (J2, J3) and adults were found in the body cavities of male and female of SPKS imagoes. J2 and J3 were commonly observed in the body cavity, while adults were rarely found. The life cycle of E. parthenonema nematodes begins with J3 exiting from SPKS imagoes and moving to the male oil palm inflorescence to infect the last instar larvae and pupae of SPKS. J3 undergoes two successive molts to become an adult nematode. E. parthenonema is parthenogenetic, so no male nematodes were found. Adult nematodes produce eggs, which then hatch into J1, remaining within the parent’s body. J2 and J3 move out into the SPKS body cavity, consume host’s organ and continuing the life cycle. Keywords: insect, pollinator, nematode, parasitic, oil palm

Ekotoksikologi Pestisida Decis 25 E, Curacron 500 EC, Score 150 EC dan Bactospein WP terhadap Lele (Clarias batrachus) dan Nila (Oreochromis niloticus) Rahayuwati, Sat
Agroprimatech Vol. 6 No. 2 (2022): Agroprimatech
Publisher : Prodi Agroteknologi Fakultas Agro Teknologi Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/agroprimatech.v6i2.3503

Aplikasi pestisida pertanian yang berlebihan dan tidak tepat dapat terbawa oleh air hujan hingga ke perairan. Decis 25E yang tergolong insektisida Pyrethroid, Curacron 500 EC termasuk insektisida golongan Organofostat, Score 150 EC fungisida sistemik dan insektisida biologi Bactospein WP diuji tingkat toksisitasnya terhadap Lele (Clarias batrachus) dan Nila (Oreochromis niloticus) pada konsentrasi 0,1 ppm; 1 ppm; 10 ppm; dan 100 ppm dengan pelarut air. Decis 25E Curacron 500 EC memiliki tingkat toksisitas tinggi terhadap Lele dan Nila yang menimbulkan pendarahan pada ikan uji dari konsentrasi 0,1 hingga 100 ppm dalam waktu lebih dari 1 jam. Sehingga adanya runoff Decis 25E dan Curacron 500 EC dengan konsentrasi 0,1 ppm di perairan dapat mengakibatkan kematian pada ikan. Fungisida Score 250 EC dan insektisida biologi Bactospeine WP cukup aman terhadap ikan lele dan nila pada konsentrasi 0,1 ppm.

Pengaruh Formulasi Cendawan Entomopatogen Metharizium anisopliae(Metchnikoff) Sorokin dan Beauveria bassiana(Bals.-Criv.) Vuill terhadap Pertumbuhan dan Daya Tahan Hama Penyakit pada Bibit Kelapa Sawit (Pre nursery) Gunawan, Juan Felix; Rahayuwati, Sat; Pratomo, Bayu; Afrianti, Suratni
Agroprimatech Vol. 8 No. 2 (2024): Agroprimatech
Publisher : Prodi Agroteknologi Fakultas Agro Teknologi Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/agroprimatech.v8i2.5409

Entomopathogenic fungi are fungi that can cause death to insect pests. Some reports state that entomopathogenic fungi are endophytic or can grow in plant tissue and are positive for plant growth and produce phenol substances that insects do not like. The study aims to determine whether the formulation of entomopathogenic fungi Metharizium anisopliae (Metchnikoff) Sorokin and Beauveria bassiana (Bals.-Criv.) Vuill given to pre-nursery oil palm seedlings can help increase growth and resistance to pests and plant diseases. The study used factorial RAK with 3 replications and 2 factors, namely M. anisopliae fungus (M) with 4 levels: M0 = 0 g (control), M1 = 5g, M2 = 10g, and M3 = 40g, and B. bassiana fungus (B) with 4 levels namely: B0 = 0g (control), B1 = 5g, B2 = 10g, and B3 = 40g. The research resultsshowed that sprouts could grow into seedlings well, with an average third leaves appearing at week 12 after planting. The results of ANOVA analysis showed that the treatment factor of M. anisopliae and B. bassiana fungus gave an effect that was not significantly different on the parameters of the number of leaves, plant height, stem diameter, leaf length, number of leaf spots, leaf spot area, number of insect bites, insect bite area, plant length, crown wet weight, crown dry weight, root wet weight, root dry weight and root crown ratio. In other words, adding various doses of mixed formulation of M. anisopliae and B. bassiana did not provide a response to trigger plant growth and prevent the undesirable effects of insects. There are suggested that the two fungi are already in the oil palm seedling tissue as endophytes but until the 12th week, they have not shown any effect as a growth promotor or preventive effect the seddling tissue not liked by insects.

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