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All Journal Jurnal Fitopatologi Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Agroplasma
Agus Eko Prasetyo
Pusat Penelitian Kelapa Sawit, Medan
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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 1 
EFFECT OF BAGWORM Pteroma pendula Joannis ATTACK ON THE DECREASE IN OIL PALM PRODUCTIVITY Priwiratama, Hari; Perdana Rozziansha, Tjut Ahmad; Susanto, Agus; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 19 No. 2 (2019): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.219101-108

Abstract

Outbreak of oil palm bagwormPteroma pendula has been reported within the last few years in several plantations in North Sumatra. A study was conductedto determine the impact of P. pendula attacks on oil palm productivity. Aerial photograph was taken in November 2016 andanalyzed to determine the level of damage on each palm canopy. The damage symptoms were classified onto healthy palms ascontrol plants (score 0), mild (score 1), moderate (score 2), severe (score 3), and very severe (score 4). The sample palms wererandomly selected and observed for the sex ratio, bunch number and bunch weight at 18 months after defoliation. The attackof P. pendula at the highest level (score 4) caused a significant effect on sex ratio and the number of bunches produced. Thefresh fruit bunches production was declined 21.02–36.35% on palms with moderate to very severe attack (score 2–4). Similarpalms also have a potential case of inflorescences abortion 18.41–32.54%. However, the average bunch weight was notinfluenced by P. pendula attack.
KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO Mutaqin, Kikin Hamzah; ., Giyanto; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.705 KB) | DOI: 10.23960/j.hptt.217105-110

Abstract

Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.
Deteksi dan Identifikasi Fitoplasma yang Berasosiasi dengan Penyakit Layu Kelapa Di Pulau Derawan, Kalimantan Timur Prasetyo, Agus Eko; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.473 KB) | DOI: 10.14692/jfi.13.3.89

Abstract

Coconut is a major commodity in Derawan island as source of additional income for the farmers. Research was conducted to detect and identify phytoplasmas associated with coconut wilt disease in Derawan island. Coconut wilt disease was indicated by the typical symptoms, i.e.  leaf yellowing, shorten of coconut sheaths and leaves, necrosis and collapse of old leaves, and also nut fall.  The presence of phytoplasmas in phloem tissues of coconut stem was observed using fluorescence and electron microscope.  Identification of phytoplasmas was carried out by nested-PCR and sequencing of the 16S rRNA gene.  DNA fragment of phytoplasma with the size of 1.25 kbp was successfully amplified using primer pairs P1/P7, followed by primer pairs R16F2n/R16R2.  Sequence analysis of the amplified fragments showed that phytoplasma associated with coconut wilt disease in Derawan island belongs to 16SrII (witches broom phytoplasma) and 16SrXI (ca. Phytoplasma oryzae) groups.
Deteksi Keberadaan Nematoda Parasit Elaeolenchus parthenonema Pada Serangga Penyerbuk Kelapa Sawit Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) dari Deli Serdang dan Simalungun Sumatera Utara Ompusunggu, Hana Herawati; Purba, Josua Aldon; Tamba, Yudi Jhosua; Rahayuwati, Sat; Pradana, Mahardika Gama; Prasetyo, Agus Eko
JURNAL AGROPLASMA Vol 12, No 2 (2025): Vol 12, No 2 (2025): (JURNAL AGROPLASMA VOLUME 12 NO 2 TAHUN 2025)
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v12i2.7599

Abstract

In Indonesia, numerous reports indicate a decline in the population of the oil palm pollinating insect (SPKS) Elaeidobius kamerunicus (Coleoptera: Curculionidae). The parasitic nematode Elaeolenchus parthenonema has not yet been investigated as a potential agent contributing to this SPKS population decline. This research aims are to determine the presence of parasitic nematodes in SPKS populations from Simalungun Regency and Deli Serdang Regency, North Sumatra Province, and to understand the life cycle of these parasitic nematodes. The parasitic nematode E. parthenonema was found, with a low infestation severity per individual SPKS: 1.9 nematodes from Simalungun and 4.94 nematodes from Deli Serdang. Juvenile nematodes (J2, J3) and adults were found in the body cavities of male and female of SPKS imagoes. J2 and J3 were commonly observed in the body cavity, while adults were rarely found. The life cycle of E. parthenonema nematodes begins with J3 exiting from SPKS imagoes and moving to the male oil palm inflorescence to infect the last instar larvae and pupae of SPKS. J3 undergoes two successive molts to become an adult nematode. E. parthenonema is parthenogenetic, so no male nematodes were found. Adult nematodes produce eggs, which then hatch into J1, remaining within the parent’s body. J2 and J3 move out into the SPKS body cavity, consume host’s organ and continuing the life cycle. Keywords: insect, pollinator, nematode, parasitic, oil palm
Co-Authors . Giyanto Hari Priwiratama Kikin H Mutaqin Ompusunggu, Hana Herawati Perdana Rozziansha, Tjut Ahmad Pradana, Mahardika Gama Purba, Josua Aldon Rahayuwati, Sat Tamba, Yudi Jhosua Wiwik Handayani