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All Journal HAYATI Journal of Biosciences Jurnal Agroplasma Jurnal Ilmiah Pertanian ( JIPERTA)
Pradana, Mahardika Gama
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Apis cerana Fabricius, 1793 in Sumatra: Haplotype Variations of Mitochondrial DNA and the Molecular Relationship with the Asian Honey Bees (Hymenoptera: Apidae) Simanjuntak, Juniarto Gautama; Priawandiputra, Windra; Raffiudin, Rika; Shullia, Nurul Insani; Jauharlina, Jauharlina; Pradana, Mahardika Gama; Meilin, Araz; Jasmi, Jasmi; Pujiastuti, Yulia; Lestari, Puji; Ilyasov, Rustem; Sitompul, Rahmadi; Atmowidi, Tri
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.768-780

Abstract

Honey bee Apis cerana is widely distributed in Asia and the Indonesian archipelago, including Sumatra. We studied the molecular variations of A. cerana using cytochrome c oxidase subunits 1 and 2 genes (cox1 and cox2) and the cox1/cox2 intergenic spacers (igs) in several altitudes in the six provinces of Sumatra. We explored the haplotype distributions of those three mtDNA markers for A. cerana in the low-, mid-, and highlands of Sumatra. We also analyzed their relationship with A. cerana in Sundaland and Asia using those markers. Our study revealed 12 new haplotypes of A. cerana cox1 in Sumatra, while nine and eight new haplotypes for cox2 and igs, respectively. Apis cerana in North Sumatra, Lampung, and South Sumatra had the three highest haplotype variations. Most of the specific haplotypes of inter-colony A. cerana from Sumatra were found in the lowlands, while most were in the highlands for intra-colony variations. We found low gene flow among populations of A. cerana in Sumatra. One haplotype, Sumatra4 cox2 from North Sumatra, was the same as Java3 haplotype, presumably due to anthropogenic impact. The molecular phylogenetic tree of A. cerana in the Sundaland revealed that A. cerana from Sumatra has a close relationship to those of Borneo compared to Java.
Deteksi Keberadaan Nematoda Parasit Elaeolenchus parthenonema Pada Serangga Penyerbuk Kelapa Sawit Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) dari Deli Serdang dan Simalungun Sumatera Utara Ompusunggu, Hana Herawati; Purba, Josua Aldon; Tamba, Yudi Jhosua; Rahayuwati, Sat; Pradana, Mahardika Gama; Prasetyo, Agus Eko
JURNAL AGROPLASMA Vol 12, No 2 (2025): Vol 12, No 2 (2025): (JURNAL AGROPLASMA VOLUME 12 NO 2 TAHUN 2025)
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v12i2.7599

Abstract

In Indonesia, numerous reports indicate a decline in the population of the oil palm pollinating insect (SPKS) Elaeidobius kamerunicus (Coleoptera: Curculionidae). The parasitic nematode Elaeolenchus parthenonema has not yet been investigated as a potential agent contributing to this SPKS population decline. This research aims are to determine the presence of parasitic nematodes in SPKS populations from Simalungun Regency and Deli Serdang Regency, North Sumatra Province, and to understand the life cycle of these parasitic nematodes. The parasitic nematode E. parthenonema was found, with a low infestation severity per individual SPKS: 1.9 nematodes from Simalungun and 4.94 nematodes from Deli Serdang. Juvenile nematodes (J2, J3) and adults were found in the body cavities of male and female of SPKS imagoes. J2 and J3 were commonly observed in the body cavity, while adults were rarely found. The life cycle of E. parthenonema nematodes begins with J3 exiting from SPKS imagoes and moving to the male oil palm inflorescence to infect the last instar larvae and pupae of SPKS. J3 undergoes two successive molts to become an adult nematode. E. parthenonema is parthenogenetic, so no male nematodes were found. Adult nematodes produce eggs, which then hatch into J1, remaining within the parent’s body. J2 and J3 move out into the SPKS body cavity, consume host’s organ and continuing the life cycle. Keywords: insect, pollinator, nematode, parasitic, oil palm
Efektivitas Cendawan Cordyceps militaris pada Berbagai Media terhadap Hama Ulat Api (Setothosea assigna L.) Ginting, Jordanta; Kuswardani, Retna Astuti; Pradana, Mahardika Gama
Jurnal Ilmiah Pertanian ( JIPERTA) Vol 8, No 1 (2026): Jurnal Ilmiah Pertanian (JIPERTA), Maret (IN Press)
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jiperta.v8i1.6602

Abstract

This research aims to determine the growth response of Cordyceps militaris in several growing media, determine the best growing media for cultivating Cordyceps militaris and determine the effectiveness of cultivating Cordyceps militaris against fire caterpillar larvae (Setothosea assigna). This research was carried out using a non-factorial Completely Randomized Design method, with 3 growing media treatments, namely N1 = Bran, N2 = Rice and N3 = Corn. The number of repetition was 6. Data analysis uses Anova, where if the results showed a significant and very siognificant effect, it was continued with Duncan’s Multiple Range Test (DMRT). The results showed that the growth medium had no significant effect on the density of yellow fungus and white fungus conidia, the viability of yellow fungus and white fungus condidia. The growth media of C. militaris is found in brand media, which is observed from the viability of fungus, both in yellow and white conidia, which can kill Setothosea assigna larvae which are marked in bran, rice and corn media at the age of 11-19 day after application, causing mortality between 93,34-100%,
Co-Authors Agus Eko Prasetyo Araz Meilin Ginting, Jordanta Ilyasov, Rustem Jasmi Jasmi Jauharlina Jauharlina Nurul Insani Shullia Ompusunggu, Hana Herawati PUJI LESTARI Purba, Josua Aldon Rahayuwati, Sat Retna Astuti Kuswardani RIKA RAFFIUDIN Simanjuntak, Juniarto Gautama Sitompul, Rahmadi Tamba, Yudi Jhosua Tri Atmowidi WINDRA PRIAWANDIPUTRA, WINDRA YULIA PUJIASTUTI