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Apis cerana Fabricius, 1793 in Sumatra: Haplotype Variations of Mitochondrial DNA and the Molecular Relationship with the Asian Honey Bees (Hymenoptera: Apidae) Simanjuntak, Juniarto Gautama; Priawandiputra, Windra; Raffiudin, Rika; Shullia, Nurul Insani; Jauharlina, Jauharlina; Pradana, Mahardika Gama; Meilin, Araz; Jasmi, Jasmi; Pujiastuti, Yulia; Lestari, Puji; Ilyasov, Rustem; Sitompul, Rahmadi; Atmowidi, Tri
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.768-780

Abstract

Honey bee Apis cerana is widely distributed in Asia and the Indonesian archipelago, including Sumatra. We studied the molecular variations of A. cerana using cytochrome c oxidase subunits 1 and 2 genes (cox1 and cox2) and the cox1/cox2 intergenic spacers (igs) in several altitudes in the six provinces of Sumatra. We explored the haplotype distributions of those three mtDNA markers for A. cerana in the low-, mid-, and highlands of Sumatra. We also analyzed their relationship with A. cerana in Sundaland and Asia using those markers. Our study revealed 12 new haplotypes of A. cerana cox1 in Sumatra, while nine and eight new haplotypes for cox2 and igs, respectively. Apis cerana in North Sumatra, Lampung, and South Sumatra had the three highest haplotype variations. Most of the specific haplotypes of inter-colony A. cerana from Sumatra were found in the lowlands, while most were in the highlands for intra-colony variations. We found low gene flow among populations of A. cerana in Sumatra. One haplotype, Sumatra4 cox2 from North Sumatra, was the same as Java3 haplotype, presumably due to anthropogenic impact. The molecular phylogenetic tree of A. cerana in the Sundaland revealed that A. cerana from Sumatra has a close relationship to those of Borneo compared to Java.
Deteksi Keberadaan Nematoda Parasit Elaeolenchus parthenonema Pada Serangga Penyerbuk Kelapa Sawit Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) dari Deli Serdang dan Simalungun Sumatera Utara Ompusunggu, Hana Herawati; Purba, Josua Aldon; Tamba, Yudi Jhosua; Rahayuwati, Sat; Pradana, Mahardika Gama; Prasetyo, Agus Eko
JURNAL AGROPLASMA Vol 12, No 2 (2025): Vol 12, No 2 (2025): (JURNAL AGROPLASMA VOLUME 12 NO 2 TAHUN 2025)
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v12i2.7599

Abstract

In Indonesia, numerous reports indicate a decline in the population of the oil palm pollinating insect (SPKS) Elaeidobius kamerunicus (Coleoptera: Curculionidae). The parasitic nematode Elaeolenchus parthenonema has not yet been investigated as a potential agent contributing to this SPKS population decline. This research aims are to determine the presence of parasitic nematodes in SPKS populations from Simalungun Regency and Deli Serdang Regency, North Sumatra Province, and to understand the life cycle of these parasitic nematodes. The parasitic nematode E. parthenonema was found, with a low infestation severity per individual SPKS: 1.9 nematodes from Simalungun and 4.94 nematodes from Deli Serdang. Juvenile nematodes (J2, J3) and adults were found in the body cavities of male and female of SPKS imagoes. J2 and J3 were commonly observed in the body cavity, while adults were rarely found. The life cycle of E. parthenonema nematodes begins with J3 exiting from SPKS imagoes and moving to the male oil palm inflorescence to infect the last instar larvae and pupae of SPKS. J3 undergoes two successive molts to become an adult nematode. E. parthenonema is parthenogenetic, so no male nematodes were found. Adult nematodes produce eggs, which then hatch into J1, remaining within the parent’s body. J2 and J3 move out into the SPKS body cavity, consume host’s organ and continuing the life cycle. Keywords: insect, pollinator, nematode, parasitic, oil palm
Efektivitas Cendawan Cordyceps militaris pada Berbagai Media terhadap Hama Ulat Api (Setothosea assigna L.) Ginting, Jordanta; Kuswardani, Retna Astuti; Pradana, Mahardika Gama
Jurnal Ilmiah Pertanian ( JIPERTA) Vol 8, No 1 (2026): Jurnal Ilmiah Pertanian (JIPERTA), Maret (IN Press)
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jiperta.v8i1.6602

Abstract

This research aims to determine the growth response of Cordyceps militaris in several growing media, determine the best growing media for cultivating Cordyceps militaris and determine the effectiveness of cultivating Cordyceps militaris against fire caterpillar larvae (Setothosea assigna). This research was carried out using a non-factorial Completely Randomized Design method, with 3 growing media treatments, namely N1 = Bran, N2 = Rice and N3 = Corn. The number of repetition was 6. Data analysis uses Anova, where if the results showed a significant and very siognificant effect, it was continued with Duncan’s Multiple Range Test (DMRT). The results showed that the growth medium had no significant effect on the density of yellow fungus and white fungus conidia, the viability of yellow fungus and white fungus condidia. The growth media of C. militaris is found in brand media, which is observed from the viability of fungus, both in yellow and white conidia, which can kill Setothosea assigna larvae which are marked in bran, rice and corn media at the age of 11-19 day after application, causing mortality between 93,34-100%,
Effect of combination of organosulfur and polyphenols organic fungicide on Ganoderma-infected oil palm seedlings Yusup, Ciptadi Achmad; Eris, Deden Dewantara; Mulyatni, Agustin Sri; Pradana, Mahardika Gama; Widiastuti, Happy; Santoso, Djoko
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.126136-146

Abstract

The curative potential of an organic fungicide against Ganoderma boninense was previously assessed in vitro and yielded promising results. This organic fungicide was formulated using a combination of organosulfur compounds and polyphenols (OSC-P) as active ingredients. This study aimed to evaluate the effectiveness of OSC-P application in controlling basal stem rot (BSR) disease in infected oil palm seedlings of different varieties and to determine the optimum application dose. Two oil palm varieties were used: a G. boninense-susceptible variety and a moderately tolerant variety. The seedlings were artificially infected with G. boninense using inoculated rubber wood blocks (RWB). OSC-P was applied to 16-week-old seedlings by soil drenching at a volume of 200 mL per plant at 2-week intervals for 24 weeks. A total of six treatments, including controls, were arranged with four replications and observed at 4-week intervals. Each replication consisted of 10 seedlings. Observed parameters included disease incidence, disease severity, and vegetative growth parameters. The results showed that OSC-P application significantly reduced the incidence and severity of BSR disease in infected oil palm seedlings. BSR symptoms were less severe in the moderately tolerant variety than in the susceptible variety; however, disease incidence did not differ significantly between the two varieties. The optimum application dose of OSC-P was 0.8% (v/v), and variations in application dose did not significantly affect disease incidence or severity at 24 weeks after application. These findings are expected to complement existing preventive strategies for BSR management.
KUMBANG MONCONG PENGGEREK TANDAN BUAH KELAPA SAWIT: IDENTIFIKASI, GEJALA SERANGAN DAN FAKTOR PREDISPOSISI Prasetyo, Agus Eko; Pradana, Mahardika Gama
WARTA Pusat Penelitian Kelapa Sawit Vol. 30 No. 2 (2025): Warta Pusat Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.war.warta.v30i2.207

Abstract

Ledakan hama penggerek tandan buah kelapa sawit seluas lebih dari 6.000 ha telah terjadi pada tanaman awal menghasilkan di Kabupaten Dharmasraya, Sumatra Barat pada pertengahan tahun 2024. Hama penggerek tandan buah tersebut terindentifikasi sebagai Rhabdocelus obscurus. Gejala serangan R. obscurus ditandai dengan adanya kerusakan yang disebabkan oleh gerekan larva pada buah, spikelet dan tangkai tandan. Ini berbeda dengan gejala kerusakan akibat hama Tirathaba mundella yang hanya berupa kerusakan pada buah saja. Gerekan larva mengakibatkan kerusakan jaringan lebih parah jika disertai dengan infeksi sekunder oleh jamur Marasmius palmivorus. Tingkat serangan hama menjadi lebih besar pada daerah dengan ketinggian tempat > 400 mdpl, curah hujan dan kelembapan lebih tinggi, serta kultur teknis terutama kastrasi, pengendalian gulma, dan sanitasi buah busuk tidak berjalan dengan baik. Tindakan pengendalian dapat dilakukan secara reaktif dengan melakukan sanitasi buah terserang berat diikuti dengan aplikasi insektisida. Tindakan pengendalian secara proaktif untuk mencegah ledakan hama dapat dilakukan dengan penerapan kultur teknis yang baik terutama pengendalian gulma, penunasan sesuai standar, dan implementasi kastrasi secara rutin setiap bulan guna mengurangi kelembapan yang menjadi faktor predisposisi utama hama.