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All Journal Biospecies Jurnal Lahan Suboptimal Biogenesis: Jurnal Ilmiah Biologi
Z, Zulkarnain
Department Of Agroecotechnology Agricultural Faculty University Of Jambi Kampus Pinang Masak, Jl. Raya Jambi – Muara Bulian Km. 15, Mendalo Indah, Jambi 36361
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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In Vitro Callus Development on Immature Leaf Explants of Liberica Coffee (Coffea liberica L. cv. Liberika Tungkal Komposit) by the Application of 2.4-D and BAP Lizawati Lizawati; Zulkarnain Zulkarnain; Neliyati Neliyati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.11769

Abstract

Conventional vegetative propagation is the process in which new plants are grown from a variety of sources, seeds, cuttings and other parts of the plants. Therefore, the conventional vegetative propagation of liberica coffee by cutting or grafting of stems is hampered by the limited number of stem or branches, which can be used as propagating materials. In addition, the tissue culture technique is another method used to propagate liberica coffee. This study aims to investigate an efficient protocol for embryogenic callus development from leaf explants of Coffea liberica cv. Liberika Tungkal Komposit. The explants used are immature leaves of fully opened liberica coffee. The medium used was Murashige and Skoog (MS) composition supplemented with vitamins, 3% sucrose and solidified with 0.7% agar, and the medium pH was adjusted to 5.8 ± 0.1. The experiment was arranged in a factorial randomized block design, and the first factor was 2.4-D (2.0, 3.0, 4.0, and 5.0 ppm) and then the second factor was BAP (0.0, 0.5, and 1.0 ppm). The results showed that the application of 2.4-D and BAP significantly improved the distribution of callus proliferation on cultured explants. The use of 2.0 ppm 2.4-D + 1.0 ppm BAP resulted in the fastest callus proliferation (19 days after culture initiation). In general, the application of different levels of 2.4-D and BAP successfully induced friable with nodular morphology callus on young leaf explant of liberica coffee. It is believe that the callus has embryogenic capacity and will undergo embryogenesis when transferred to a suitable medium composition
Improving Drought Tolerance in Chili Pepper by the Application of Local Microorganisms (LMo) Eliyanti Eliyanti; Zulkarnain Zulkarnain; Budiyati Ichwan; Trias Novita; Janes Sihotang
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 11 No. 1 (2022): JLSO
Publisher : Research Center for Sub-optimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jlso.11.1.2022.566

Abstract

his study aimed at determining the effect of spraying intervals of local microorganism (MoL) golden apple snails on the improvement of drought tolerance of chili grown under limited water availability. The trial was carried out at greenhouse and Plant Protection Laboratory, Faculty of Agriculture, University of Jambi for 6 months. The study used a Randomized Block Design with 2 factors and 3 replications. The first factor was the chili cultivar consisted of Vitra, Lado and Laris. While the second factor was the spraying interval of MoL golden apple snail consisted of 100% field capacity of soil water without MoL application, 75% field capacity of soil water and spraying intervals once a week, and 75% field capacity of soil water and spraying intervals every two weeks. The results showed that spraying MoL golden apple snail at intervals of either once in 1 or 2 weeks could increase tolerance to drought stress in three chili cutivars grown under 75% field capacity of soil water. Plant growth and yield at 75% field capacity of soil water was the same as that of plants grown under optimum conditions (100 % field capacity of soil water), MoL spray on the cv. Vitra could increase fruit weight per plant by up to 32% over fruit weight of those plants grown under optimum conditions.
Growth and Yield of Shallots at various Plant Spacing in Ultisol Dry Land Budiyati Ichwan; Eliyanti Eliyanti; Zulkarnain Zulkarnain; Susilawati Susilawati; Indra Permana
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 12 No. 1 (2023): JLSO
Publisher : Research Center for Suboptimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jlso.12.1.2023.622

Abstract

Plant spacing was one method that could be used to boost plant populations. In order to increase shallot (Allium ascalonicum L.) production and productivity on less-than-ideal ground, such as dry land in Jambi. This study aimed to examine the effects of various plant spacing on the growth and yield of shallots and to obtain plant spacing that provides the best shallot growth and yield. This research was conducted at the Teaching and Research Farm, Faculty of Agriculture, University of Jambi. The design used a   Randomized   Block   Design (RBD) consisting of four treatments with six replications. The treated plant spacing was:10 cm x 10 cm; 15 cm x 15 cm; 15 cm x 20 cm; and 20 cm x 20 cm. Various plant spacing affected the shallot growth and yield, and a plant spacing of 15 cm x 15 cm provided better growth and yield compared to any other plant spacing, with the number of leaves per plant, the weight of bulbs per clump, and the weight per bulb of 22, 49, 49.84, and 13.94 g   successively.
Pakcoy's (Brassica rapa L.) Response to the Provision of Liquid Organic Fertilizer in Polybags Budiyati Ichwan; Irianto Irianto; Eliyanti Eliyanti; Mapegau Mapegau; Zulkarnain Zulkarnain; Ikhbal Ikhbal
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 12 No. 2 (2023): JLSO
Publisher : Research Center for Suboptimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jlso.12.2.2023.631

Abstract

This study aimed to examine the effect of Liquid Organic Fertilizer (LOF) in increasing the growth and yield of pakcoy, as well as obtaining LOF concentrations that provided the best growth and yields of pakcoy plants. It was conducted at the Teaching and Research Farm, Faculty of Agriculture, University of Jambi, 35 asl. The study used a Randomized Block Design with one factor, namely various LOF concentrations: 0ml/l; 5ml/l; 10ml/l, 15ml/l. Each treatment was repeated five times. The results showed that LOF application was able to increase the growth and yield of pakcoy plants in the form of plant height (4.94%–24.49%), number of leaves (4.63%–9.26%), leaf area (18.17%–46.12%), dry weight (50.98%–94.90%), and plant fresh weight (50.46%–102.60%). The LOF concentration of 10ml/l provided the best pakcoy growth and yield. The results of this study have just been able to produce a fresh weight of pakcoy 26%–33% of the potential pakcoy yield.
Mengukur viabilitas serbuk sari dan sukrosa terhadap perkecambahan Swainsona formosa (G.Don) J.Thompson Zulkarnain Z
Biospecies Vol. 12 No. 1 (2019): Januari 2019
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.512 KB) | DOI: 10.22437/biospecies.v12i1.6596

Abstract

The work presented here was aimed at investigating the proper time (after spreading) for viability assessment and the effect of various sucrose concentrations on pollen germination in Swainsona formosa. The rate of pollen tube formation was determined for freshly shed pollen grains of glasshouse-grown plants at 10, 15, 20, 25, 30, 60 and 120-minute intervals after being plated on Brewbaker and Kwack (BK) medium. The results indicated that within 60 minutes pollen germination reached 63.70%, after which remained steady at 120 minutes (63.71%). Under the effect of various concentrations of sucrose, i.e. 0.5, 10, 15, and 20% (w/v), the germination rate of pollen grains was assessed at 60 minutes following germination. The results showed that sucrose concentration of 10 – 15% (w/v) produced better germination rate (64.14%) compare to lower concentrations (19.64 and 43.58% at zero and 5% sucrose, respectively). Sucrose concentration above 15% was also found to inhibit pollen germination (48.92% at sucrose concentration of 20%).
Co-Authors Budiyati Ichwan Dr. Susilawati Susilawati Eliyanti Eliyanti Ikhbal Ikhbal Indra Permana Irianto Irianto Janes Sihotang Lizawati Lizawati Mapegau Mapegau NELIYATI, Neliyati Trias Novita