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All Journal Mutiara Medika: Jurnal Kedokteran dan Kesehatan Biomedical Journal of Indonesia Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Bioscientia Medicina : Journal of Biomedicine and Translational Research Scientia Psychiatrica Open Access Indonesia Journal of Social Sciences Sriwijaya Journal of Ophthalmology Eureka Herba Indonesia Indonesian Community Empowerment Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Open Access Indonesia Journal of Social Sciences Journal of Anesthesiology and Clinical Research
Patricia Wulandari
Cattleya Mental Health Center, Palembang, Indonesia
Religion Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 5 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i5.228

Abstract

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.
Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 4 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v5i4.229

Abstract

Western blotting is an important technique used in cell and molecular biology. Using the western blot, researchers can identify specific proteins from the complex mixture of proteins extracted from cells. This technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target proteins using appropriate primary and secondary antibodies to visualize. This paper will attempt to explain the techniques and theory behind western blot, and offer several ways to solve the problem
Preclinical Test Methods with Animal Mouse and Rats Biomedical Research: Technical Guideline Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 2 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i2.232

Abstract

A B S T R A C TTrials in pre-clinical trials are very complex multidisciplinary studies. Extrapolatingdata from animals to humans requires information from many fields of science. Frompre-clinical trials, more detailed information and data on efficacy and safety wereobtained, especially at doses equivalent to doses in humans, and the presence orabsence of cumulative effects and whether these effects could return to normal(reversible) after the administration of the test material was stopped. This test canbe used to predict the negative impact on humans if he is exposed to the materialfor a long time.
Euthanasia Procedure of Animal Model in Biomedical Research Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 6 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i6.310

Abstract

Regardless of the method, it is essential to induce unconsciousness as rapidly as possible if euthanasia is to be aesthetically and scientifically successful. Criteria that have been considered in recommending the methods of euthanasia include: time required to produce unconsciousness, time required to produce death, purposes, research results and compliance with the AVMA Guidelines on Euthanasia. There are special considerations for euthanizing rodent embryos, fetuses and neonates.
Protocol for Anesthesia Animal Model in Biomedical Study Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 7 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i7.311

Abstract

Recognition of pain depends upon intact pathways from pain receptors to the thalamus and cerebral cortex, as well as functional cerebral cortex and subcortical structures. Thus any means that renders the cerebral cortex nonfunctional, such as hypoxia or drug depression, prevents pain. When this happens, stimuli that evoke motor nerve reflexes that may be painful to the conscious animal are not painful in the unconscious animal. Equally painful stimuli administered to animals chemically paralyzed by curare or succinylcholine will not evoke a motor reflex simply because of paralysis, but will cause pain because of the conscious state. Hence, it is possible that unconscious animals may feel no pain but respond to certain stimuli, and paralyzed animals may feel pain but cannot respond.
Fluid and Drug Administration Procedure Animal Model in Biomedical Research Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i8.312

Abstract

Drugs, vaccines, injectable anesthetics or other agents are to be administered, one or more of several different routes may be selected. The routes selected are governed by the nature of the agent being administered, the animal, the purpose of the administration and other factors.
Handling of Common Laboratory Animals in Biomedical Study Rachmat Hidayat; Patricia Wulandari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 9 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i9.313

Abstract

Mice are usually caught and lifted by the tail. The tail should be grasped abouttwo-thirds of the way down. With this simple method of holding, they may betransferred to another cage or a balance, identified or sexed; but such restraint isnot sufficient for treatment and close examination. For more effective control, themouse may be held by the tail and placed on a table or other surface, preferablyone that the mouse can grasp, and the loose skin over the neck and shouldersgrasped with thumb and fingers.
Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue Patricia Wulandari
Eureka Herba Indonesia Vol. 1 No. 1 (2020): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v1i1.2

Abstract

Noni fruit (Morinda citrifolia) is one of the most common plants in Indonesia. This plant is often found in various regions in Indonesia, where these plants are often in the form of shrubs or wild plants that grow in yards or plantations. This study aims to assess the effect of noni (Morinda citrifolia) fruit extract on blood sugar levels and the expression of GLUT4 protein in muscle tissue which shows the potential of the test extract's ability to improve blood glucose intake in cells so that it can maintain blood sugar regulation. This research was an experimental study that used white rats as research subjects. Induction of diabetes was done by injecting alloxan at a dose of 110 mg/kg BW intraperitoneally; then the white rats were given 10% glucose to drink. Alloxan-induced white rats showed a very significant increase in blood sugar levels, where the use of the drug metformin was able to reduce blood sugar levels significantly. In white rats induced with diabetes mellitus, there was a decrease in GLUT4 levels in muscle tissue. The administration of metformin drugs or noni fruit extracts shows the ability to increase levels of GLUT4 protein. In conclusion, noni fruit extract affects lowering blood sugar levels in diabetes mellitus white rats by increasing glucose intake in cells and tissues.
Safety Assessment of Combination of Tinospora crispa Extract, Andrographis paniculata, Cinnamomum burmanii, Syzygium polyanthum and Momordica charantia in Hepar and Renal Function Rachmat Hidayat; Patricia Wulandari
Eureka Herba Indonesia Vol. 2 No. 1 (2021): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v2i1.13

Abstract

Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia contain phenolics and flavonoids which are rich in benefits. Phenolic compounds are compounds that have an aromatic ring with one or more hydroxy groups, compounds derived from plants that have the same characteristics, namely aromatic rings containing one or more hydroxyl groups. Herbal plants, which are rich in phenolic compounds, have the efficacy, among others, as antioxidants, anti- inflammatory, antimicrobial, anti-fungal and prevents tumor growth. The acute toxicity test is a procedure designed to determine the median lethal dose of a substance and its possible mechanism of action and target organs. In simple terms, toxicity can be defined as the ability of a compound that can cause harmful effects or deviate from the biological mechanism in an organism.
Potential Combination of Tinospora crispa, Andrographis paniculata, Cinnamomum burmanii, Syzygium polyanthum, and Momordica charantia Extracts Against Intake of Glucose in Muscle Rats-Induced Diabetes Mellitus Rachmat Hidayat; Patricia Wulandari
Eureka Herba Indonesia Vol. 2 No. 1 (2021): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v2i1.14

Abstract

Diabetes mellitus is a chronic condition that disturbs the body's blood sugar regulation. This disorder is characterized by a decrease in the ability of body cells to intake glucose into cells. Due to the failure of cells in glucose intake, glucose buildup occurs in the extracellular, namely in the plasma. These medicinal plants contain quite varied secondary metabolites, and these plants are rich in flavonoids, alkaloids, terpenes, and glycosides. The content of these secondary metabolite compounds is believed to be rich in antioxidant effects so that it has the effect of being able to suppress various oxidative stress conditions that cause damage to various organs due to blood sugar dysregulation. The effect of Tinospora crispa, Andrographis paniculata, Cinnamomum burmanii, Syzygium polyanthum and Momordica charantia extract on blood sugar levels and GLUT4 protein expression in muscle tissue shows the potential of the test extract's ability to improve blood glucose intake to cells so as to maintain blood sugar regulation.
Co-Authors Andrian F Kusumadewi Budi Pratiti Budi Pratiti Carla R Marchira Carla R Marchira Carla Raymondalexas Marchira Cecep S Kristanto Despian Januandri Edith Humries Ester G Panserga Farouk, Husnil Mischa Chantal Adella Muhammad, Wahyudo Imami Ni Made Nova Indriyani RACHMAT HIDAYAT Rachmat Hidayat Rachmat Hidayat Rarum, Anovy Ridwan, Hibsah Shinta Anggraini, Shinta Zahra D Khan