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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Biospecies Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Jurnal Media Pertanian Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian Jurnal Silva Tropika Jurnal Karya Abdi Masyarakat Jurnal Ilmiah Ilmu Terapan Universitas Jambi
Neliyati, Neliyati
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Journal : Biospecies

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Induksi kalus eksplan daun Anggrek Pensil (Papillionanthe hookeriana Rchb.f.) pada Kombinasi Zat Pengatur Tumbuh 2,4-D dan BAP Neliyati, Neliyati; Lizawati, Lizawati
Biospecies Vol. 17 No. 1 (2024): Januari 2024
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v17i1.31492

Abstract

The formation of callus is one of the indications of success in propagation through tissue culture. A crucial factor in callus induction is the accuracy in determining the type and concentration of auxin and cytokinin growth regulators in the culture media. This research aims to obtain the appropriate concentration of auxin (2,4-D) and cytokinin (BAP) growth regulators that can induce the formation of embryonic callus propagules from pencil orchid plants using tissue culture propagation techniques. The treatments tested in this study were the application of several concentrations of auxin (2,4-D) at 0.5; 1.0; 1.5, and 2.0 ppm combined with cytokinin (BAP) at concentrations of 0.0 and 0.5 ppm. The experiment was arranged in a Completely Randomized Design (CRD) with 15 explants for each treatment. The parameters observed were the response time of the explant (swelling), the percentage of explants swelling, the appearance of callus, the percentage of explants forming callus, callus structure, and callus color. The results showed that the pencil orchid leaf explants responded by swelling and forming callus. Swelling occurred fastest two weeks after culturing. The highest percentage of swelling and callus formation was obtained from the treatment of MS media supplemented with a combination of 2,4-D 2.0 ppm + 0.5 ppm cytokinin BAP, with 100% of the explants swelling and 26,67% forming callus. The formed callus was friable in structure and white in color.
OPTIMIZATION OF CACAO SEEDLING GROWTH (Theobroma Cacao L.) THROUGH THE INTERACTION OF Asystasia gangetica COMPOST FERTILIZER AND NPK FERTILIZER Prasatyio, Ahwa Eko; Gusniwati, Gusniwati; Fathia, Nyimas Myrna Elsa; Neliyati, Neliyati
Biospecies Vol. 18 No. 2 (2025): Juli 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i2.44581

Abstract

Cacao (Theobroma cacao L.) is a widely cultivated plantation commodity. However, its production has declined, with one of the contributing factors being old plants, so rejuvenation must be carried out using high-quality plant seedlings. Good-quality seedlings are obtained through the addition of nutrients during the nursery phase using organic and inorganic fertilizers. This study aims to determine the interaction between Asystasia compost and NPK on seedling growth and to obtain the best dosage of Asystasia compost and NPK on the growth of cacao (Theobroma cacao L.) seedlings. This research was conducted using a Completely Randomized Design (CRD) with a factorial pattern consisting of two factors. The first factor was the dose of NPK fertilizer (P), which consisted of three levels: p0 = 0 g/seedling, p1 = 7 g/seedling, and p2 = 14 g/seedling. The second factor was the dose of Asystasia compost (A), which also consisted of three levels: a0 = 83 g/seedling, a1 = 166 g/seedling, and a2 = 249 g/seedling. There were nine treatment combinations, each repeated three times. In this experiment, cacao seedlings were planted in polybags. The data obtained were analyzed using analysis of variance (ANOVA) to determine the effects, followed by the DNMRT test. The observed variables were seedling height, stem diameter, number of leaves, total leaf area, shoot dry weight, and root dry weight. The results showed that there was an interaction between the application of Asystasia compost and NPK on the variables of seedling height, total leaf area, and shoot dry weight. The application of 166 g/seedling of Asystasia compost and 7 g/seedling of NPK showed the best growth of cacao seedlings in polybags.
Co-Authors ANWAR, DEDY Arif Rohman Elly Indra Swari Elly Indra Swari Fathia, Nyimas Myrna Elsa Gusniwati , Herni Dwinta Pebrianti, Herni Dwinta Hutwan Syarifuddin Jasminarni Lizawati Lizawati Nainggolan, Ellyas Alga Nerty Soverda Prasatyio, Ahwa Eko Putri, Desma Ramadhan, Febri RR. Ella Evrita Hestiandari Setiyawati, Ida Swari, Elly Wahyuni, Dilla Wari, Elli Indras Wibowo, Yudha Gusti Yuni Ratna Zulkifli Zulkifli