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Effect of oxygen hyperbaric therapy on malondialdehyde levels in saliva of periodontitis patients with type 2 diabetes mellitus Dian Mulawarmanti; Widyastuti Widyastuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 4 (2008): December 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Lipid peroxidation (LPO) has implication in pathogenesis of several pathological disorders including periodontitis. Malondialdehyde (MDA) is end products of upid peroxidation. Hyperbaric Oxygen Therapy (HBOT) involves the administration of 100% oxygen under atmosphere pressure and has been used as an adjuvant therapy, while saliva is a diagnostic tool for many oral and systemic diseases. Purpose: The aim of this study was to examine the effect of HBOT on malondialdehyde in saliva to measure lipid peroxidation in periodontitis patients with type 2 Diabetes Mellitus (DM). Methods: Eight regulated type 2 DM subjects were compared to ten unregulated periodontitis patients type 2 DM (n = 18). Pre HBOT and after 10 days HBOT with 2.4 ATA dose, unstimulated whole saliva samples from study subjects were collected, centrifuged at 3000 g for 15 minutes and were then stored at −80° C until analyzed. The MDA level was determined with 2-thiobarbituric acid by a colorimetric method at 532 nm. Results: Data showed that regulated type 2 DM had lower level of MDA (3.08 ± 0.62 ug/mol) compared with unregulated periodontally- type 2 DM subjects (5.88 ± 1.04 ug/mol) (p>0.05). MDA levels were significantly lower after HBOT in regulated DM (2.30 ± 0.46 ug/mol) compared with unregulated periodontally type 2 DM (4.09 ± 0.77ug/mol) (p < 0.05). The regulated DM subjects and post HBOT showed MDA levels lower than the periodontally-unregulated group significantly. Conclusion: The saliva of periodontitis patients with unregulated type 2 DM showed more lipid peroxidation than regulated DM type 2. HBOT decreased MDA levels in regulated and unregulated type 2 DM with periodontitis.

Management of palatal perforation in systemic lupus erythematosus patient Dwi Setianingtyas; Paulus Budi Teguh; W Widyastuti; Neken Prasetyaningtyas; Ramadhan Hardani Putra; Felicia Eda Haryanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 2 (2018): June 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i2.p62-66

Background: Systemic Lupus Erythematosus (SLE) is an autoimmune disease which damages tissues and causes chronic inflammation with an idiopathic etiology. It has been suggested that oral lesions in patients with SLE can be grouped clinically as erythema, discoid lesions and oral ulcerations. The latter have been said to indicate the onset of a severe systemic disease flare and that oral ulcers represent cases of mucosal vasculitis. Palatal lesions generally present in the form of ulcers or, in more severe forms, as perforation. Acquired palatal perforations can be caused by several etiologies including: developmental disorders, malignancy and infections. Purpose: To report the management of palatal perforation in an SLE patient. Case: A 14-year-old female patient attended the Dr. Ramelan Naval Hospital, with both a perforated palate that often caused her to choke when eating or drinking and maxillary anterior tooth mobility. Case Management: The treatment for the patient in this case consisted of debridement and DHE, pharmacological therapy including aloclair gel and minosep mouthwash to maintain oral hygiene and prevent re-infection. At the end of the first consultation, the patient was prescribed an obturator in order to avoid oro-anthral infection. During the second consultation, the patient’s orthodontic bracket was removed to facilitate scaling and splinting of the anterior maxillary teeth carried out to prevent their movement. During the third consultation, a swab was taken by an oral surgeon who also administered antifungal therapy. During the fourth and final consultation, the patient was examined a prosthodontic specialist due to an obturator which was causing discomfort. Conclusion: The management of palatal perforation lesions in an SLE patient requires a multidisiplinary approach.

Lemuru fish oil gel as host modulation therapy in periodontal ligaments induced with Porphyromonas gingivalis W. Widyastuti; Dian Widya Damaiyanti; Dian Mulawarmanti; Cindy Aprilia Sari; Diah Ayu Siwi
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 4 (2020): December 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i4.p229-234

Background: Periodontitis affects approximately 20%–50% of the global population and is caused by gram-negative bacteria, such as Porphyromonas gingivalis (P. gingivalis). Host modulation therapy (HMT) is part of a periodontal therapy that is used as an adjunct to conventional periodontal treatment to reduce tissue damage. Lemuru fish oil containing EPA and DHA can reduce the formation of MMPs and will further increase the number of fibroblasts there by stimulating collagen formation. Purpose: To determine the effect of lemuru fish oil gel on the collagen density and width of the periodontal tissue induced by P. gingivalis and the correlation between these parameters. Methods: Thirty male Wistar rats were divided into five groups. Induction of P. gingivalis was carried out first, then lemuru fish oil gel was applied to the gingival sulcus for 14 days, according to collagen scores in histological preparations using Masson's trichrome (MT). The width of the periodontal ligament was measured with an image raster program in µm. The data were analysed using statistics to test hypotheses using SPSS version 24. Results: Significant differences in the results of the collagen density were observed between groups K- and K+ and groups K+ and P2. Meanwhile, no significant difference was observed between groups K- and P2, P3, P2 and P3 and K+ and P1. The mean values of the periodontal ligament widths were K- (299.61 ± 51.82µm), K+ (425.85 ± 61.54µm), P1 (346.93 ± 33.53µm), P2 (370.15 ± 49.42µm) and P3 (379.6 ± 49.26). Conclusion: Lemuru fish oil can affect the width of the ligament and the collagen density with an optimal concentration of 20%. The correlation between the collagen density and the periodontal ligament width was negative and not significant.

The effectiveness of the combination of Moringa oleifera extract and propolis on Porphyromonas gingivalis biofilms compared to 0.7% tetracycline Hansen Kurniawan; W. Widyastuti; Mery Esterlita Hutapea
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 2 (2021): June 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i2.p63-67

Background: Periodontitis is an inflammatory disease that occurs in periodontal tissues. Porphyromonas gingivalis is also known as a bacterium commonly associated with the pathogenesis of periodontitis. Tetracycline is one of the antibiotics often used in periodontal tissue treatment. Propolis and Moringa oleifera are also known to have certain compounds assumed to be able to inhibit biofilm growth. Purpose: This study aims to understand the effectiveness of the combination of Moringa oleifera and propolis on porphyomonas gingivalis biofilms compared to 0.7% tetracycline. Methods: A biofilm inhibition activity test was performed using the broth micro dilution method. First, bacteria were prepared by making a suspension in brain heart infusion media and adjusting it to 0.5 McFarland I standard. Second, fifteen samples were divided into five groups; group K as control group (0.1% sodium carboxymethyl cellulose), T (0.7% tetracycline), and treatment groups with the combination of propolis and Moringa oleifera in various concentrations, such as P1(10%+20%), P2(10%+40%), and P3(10%+80%). Third, the result data obtained in the form of optical density (OD) was read by using an ELISA reader. Next, statistical analysis using analysis of the variance test was conducted (p<0.05. Results: There was no significant difference between group T and group P1 (0.075). Nevertheless, there were significant differences between group T and group P2 as well as between group T and group P3 (0.00) (p=< 0.05). Conclusion: The combination of 10% propolis and 40% Moringa oleifera as well as the combination of 10% propolis and 80% Moringa oleifera have better antibacterial effectiveness against Porphyromonas gingivalis biofilm than 0.7% tetracycline.

Efektivitas Kombinasi Pemberian Minyak Ikan Lemuru (Sardinella longiceps) dan Aplikasi Hidroksiapatit terhadap Ekspresi FGF-2 pada Proses Bone Healing Tiaranita Ramadhani; Rima Parwati Sari; Widyastuti
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Bone healing on defect requiring the cells to form new bone. Fibroblast growth factor -2 belong to one of signaling control that play part in differentiation of fibroblast to osteoblast cell. Hydroxyapatite application on bone healing will aid bone regeneration as structure. Application of Sardinella longiceps oil that contain EPA and DHA as immunodulator could accelerate and optimize inflammatory phase in bone healing. Purpose: The aimed of this study to determine the effectivity of the combination of Sardinella longiceps oil and application of hydroxyapatite on the expression of fibroblast growth factor -2 (FGF-2) in bone healing process. Materials and Methods: The experimental units (male wistar rat, n=20) were devided into two groups, control (K) and treathment (P) groups. Treathment groups were given Sardinella longiceps oil per oral seven days before the surgery to make the defect, and continued for seven days later. Control groups were not given Sardinella longiceps at all. All of the wistar rat were have two defects in their right femur, which is use hydroxyapatite (K+ and P2) and no use hydroxyapatite (K- and P1). Defect was made as big as half diameter of round bur in femur. Rats were sacrificed seven days after surgery, and femur were sliced transversally and performed immunohistochemistry staining with Anti-bFGF Polyclonal antibody (Bioss®). Examination were done under light microscope. Data were analyzed using one-way Anova for FGF-2 expression. Result: Sardinella longiceps oil increased the mean of FGF-2 expression on P2 group : 15,29±5,251, compare to K- : 5,57±2,070 , K+ : 12,14±3,976 and P1 : 8,14±1,676 (P<0.05). Conclusion: The combination of Sardinella longiceps oil and hydroxyapatite application effective the expression of fibroblast growth factor-2 in the process of bone healing.

Efek Terapi Sardinella longiceps Terhadap Tinggi Tulang Kortikal Mandibula Tikus Model Periodontitis Nur Octavia; Widyastuti; Dianty Saptaswari; Dian Widya Damaiyanti
Denta Journal Kedokteran Gigi Vol 13 No 2 (2019): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis begins with accumulation of pathogenic plaque containing bacteria and toxins. Subgingival bacteria found in chronic periodontitis are one of them is Porphorymonas gingivalis. Porphyromonas gingivalis bacteria causes progressive damage to the supporting tissues of the teeth, especially the alveolar bone. Alternative medicine that can be used is with herbal ingredients, namely Sardinella longiceps. Sardinella longiceps contains omega 3 (DHA and EPA) which inhibits proinflammatory cytokines thereby accelerating the process of bone regeneration. Objective: This study aims to determine whether the administration of gel (Sardinella longiceps) affects the height of the cortical bone of the mandibular periodontitis-induced rat Porphyromonas gingivalis.Research Methods: 25 male Wistar rats were divided into 5 groups. P0 is the normal group that is not treated. P + is a group induced by Porphyromonas gingivalis. P1, P2, P3 are the groups induced by Porphyromonas gingivalis and given Sardinella longiceps gel with concentrations of 10%, 20%, 40% respectively. On the 55th day all model mice were treated and mandibular cortical bone height was measured using a digital caliper in the interdental region of the 1st molar and left lingual molars from the apex of the alveolar bone to the mandibular base. The data obtained were analyzed by one way ANOVA followed by LSD (p = 0.05). Results: Statistical tests showed the mean height of mandibular cortical bone was P0 (5.21 ± 0.21), P + (4.67 ± 0.25), P1 (5.12 ± 0.14), P2 (5.51 ± 0.12), and P3 (5.88 ± 0.13). There was a significant difference (p <0.05) between treatment groups and positive control groups (p <0.05). Conclusion: Sardinella longiceps affects the height of the cortical bone of the periodontitis rats model induced by Porphyromonas gingivalis bacteria. The most effective in the group of sardinella longiceps gel with a concentration of 40%.

Bioviability of Mangrove Leaves Extract (Sonneratia alba) on Fibroblast BHK-21 Cell Culture Arlita Gladys Tricia Charyadie; Aprilia; Widyastuti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba has been proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. Purpose: The aim of this study is to determine the cell viability of fibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. Materials and Methods: The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. Results: Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). Conclusion: In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells.

(Study of Hyperbaric Oxygen Theraphy and Gold sea Cucumber (Stichopus Hermanii) 3% for Collagen Density of Rat’s with Diabetes Mellitus Induced by Porphyromonas Gingivalis Bactery Dyah Lestarining R; Dian Mulawarmanti; Widyastuti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis is complication of DM in chronic state. Periodontitis susceptibility increase threefold in patient DM compared non-DM. Debridement with SRP alone is less effective in DM. Hyperbaric Oxygen Therapy (HBO) has the effect of increasing insulin sensitivity and synthesis of collagen. Collagen is the largest extracellular matrix supporting periodontal tissue. Stchopus hermanii contains condrotin sulfate serves to inhibit the formation of mixed periodontopatogen bacteri. Purpose: To determine the therapeutic effects of HBO 2,4 ATA 3x3’ interval 5’ 7 days and stichopus hermanii against periodontal ligament collagen density in the healing process of periodontitis with DM. Materials and Methods:The experiment design is factorial design. Rats are inducted with STZ 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis, 20 male Wistar rats were divided into 4 groups. K0 (control group), K1(STZ+P. gingivalis), K2(STZ+P. gingivalis+Stichopus hermanii), K3(STZ+P. gingivalis+HBO), and K4(STZ+P.gingivalis+stichopus hermanii HBO) after treatment on day 52, all groups were sacrificed and done making preparations for the HPA of the mandible bone. Results: Data were analyzed using chi-square, Kruskal wallis, and Mann Whitney U with 95% significance level (p = 0.05). increase level of collagen significantly K1(1 ± 0.00) - K2(2±0.00), dan K1(1 ± 0.00) - K4(2.5 ± 0.289).Conclution:Therapy HBO combination of 2.4 ATA 3 x 30 with 5 minutes air breaks for 7 days and gel Stichopus hermanii3% effective on the Densityof collagen in the periodontal ligament healing process periodontitis with DM.

Bioviabilitas Hidroksiapatit Ekstrak Cangkang Kerang Darah (Anadara granosa) Terhadap Sel Punca Mesenkimal Sebagai Bahan Graft Tulang Arlita Dewi Nastiti; Widyastuti; Fanny M Laihad
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: One of a bone defect fillers is ahydroxyapatite. Hydroxyapatite can be obtained from Anadara granosa shells extract that have high calcium content. The mineral content can be used as a bone filler material for bone grafting. However, the material is not known bioviability to against periodontal tissues so that was needed bioviability testing. This bioviability testing using mesenchymal stem cells as mesenchymal stem cells can differentiate into periodontal tissues. Purpose: To determine bioviability Anadara granosa shell extract on mesenchymal stem cells. Materials and Methods: This study was conducted using post-only control group design. Mesenchymal stem cells in 96 wells were divided into a control group of cells (n=7), media controls (n=7), and treatment (n=7). The treatment group were given various doses of the Anadara granosa shell extract with a concentration 54 mg/ml, 27 mg/ml, 13.5 mg /ml and 6.75 mg/ml. The mesenchymal stem cells were incubated for 24 hours before and after treatment. Once given MTT, the optical density is read by ELISA reader and calculated the percentage of viability. The cell viability data were analyzed with KruskalWallis statistical test, Mann-Whitney. Results: From the results showed that an increase in cell viability to Anadara granosa shell extract. Increased cell viability starting treatment group concentration of 54 mg/ml (23,,67%), 27 mg/ml (57,43%), 13,5 mg/ml (68,87%), 6,75 mg / ml (81,92%). The highest cell viability at concentrations of 6,75 mg/ml (81,92%). Conclusion: Bioviability extract blood clam Anadara granosa shell have the highestconcentration of 6,75 mg/ml and bioviabilitas lowest cell at a concentration of 54 mg/ml.

Daya Hambat Ekstrak Teripang Pasir (Holonthuria scabra) Terhadap Pertumbuhan Bakteri Mixed periodontopatogen In Vitro Susi Krestiana; Widyastuti; Vivin Ariestania
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Periodontitis is caused by the occupation of gram-negative bacteria (mixed periodontopatogen) in oral cavity. Despite causing resistance, antibiotics are still used as a therapy for periodontitis. Sand sea cucumber (Holonturia scabra) extract hold somepotentials, if developed, to become the alternative therapy for periodontitis due to its antibacterial effects against gramnegative bacteria. Purpose: To determine sand sea cucumber extract (Holonturia scabra) in inhibiting the growth of mixed periodontopatogen bacteria. Materials and Methods: This study used a research design the post-test only control group design. 40 samples of BHI agar plates, contained mixed periodontopatogen bacteria samples, were divided into 5 groups samples. K1 (negative control) was given distilled water containing filter paper, K2 (positive control) was given the antibiotic tetracycline disc 30 g, while P1, P2, and P3 were given a filter paper containing sand sea cucumber extract with a concentration of 150 g/ml, 300 g/ml, and 450 g/ml. All sample from each group were anaerobically inoculated for 2x24 hours. After inoculation, the area aroundthe filter paper will appear to be clear and it will be measured with digital calipers (mm). The acquired data will be analyzed using the Kruskal-Wallis and Mann-Whitney test. Result: The inhibition rate of K1 (6,00 ± 0,00), K2 (28,975 ± 1,20049), P1 (6,8525 ± 0,30156), P2 (7,6237 ± 0,51281), and P3 (8,75 ± 0,3525). Conclusion: Sand sea cucumber extract (Holonthuria scabra) can inhibit the growth of Mixed periodontopatogen bacteria.

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