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All Journal Agrikultura AGRITROPICA : Journal of Agricultural Sciences
Nadrawati, Nadrawati
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Agriculture, Biological Sciences & Forestry

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Deteksi dan Identifikasi Begomovirus pada Tembakau yang Ditanam Tumpang Sari dengan Tanaman Cabai Sutrawati, Mimi; Nadrawati, Nadrawati; Djamilah, Djamilah; Aulia, Ewa
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.47941

Abstract

Beragam spesies Begomovirus telah dilaporkan di berbagai daerah di Indonesia. Gejala penyakit mosaik menguning akibat infeksi Begomovirus telah dilaporkan pada berbagai tanaman budidaya di Bengkulu antara lain cabai, mentimun, labu, melon, pepaya, dan gulma. Tanaman tembakau banyak ditanam sebagai tanaman pinggir di lahan budidaya cabai. Berdasarkan pengamatan di lahan budidaya cabai di Kabupaten Kepahiang, Bengkulu ditemukan tembakau bergejala keriting dan mosaik kuning. Tujuan penelitian ini adalah mendeteksi dan mengidentifikasi Begomovirus penyebab penyakit mosaik menguning tembakau di Bengkulu. Deteksi dan identifikasi virus dilakukan dengan menggunakan metode polymerase chain reaction (PCR) menggunakan primer universal yang mengkode transcriptional activator protein (TrAp) dan replication-associated protein (Rep) dengan target fragmen ± 900 pb. Hasil amplifikasi PCR menunjukkan adanya pita target ± 900 pb pada sampel daun tembakau. Produk PCR kemudian dikirim ke First Base, Malaysia untuk dilakukan sikuensing. Begomovirus yang menginfeksi tembakau  menunjukkan homologi 99% dengan isolat Pepper Yellow Leaf Curl Indonesia Virus (PYLCIV) pada cabai di Bali (nomor aksesi LC381263). Hasil identifikasi ini merupakan laporan pertama infeksi PYLCV pada tanaman tembakau di Bengkulu.
Soil Arthropods Diversity in Sungai Suci Bengkulu Ecosystem Fauzi, Ariffatchur; Zarkani, Agustin; Putra, Apriza Hongko; Ifebri, Rihan; Wibowo, Hariz Eko; Putri, Elsa Lolita; Nadrawati, Nadrawati
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.1.23-27

Abstract

Sungai Suci is a part of Bengkulu that is 400 meters above sea level. Sungai Suci is located in a coral coastal area with three ecosystems: the bamboo ecosystem and the sea pine ecosystem; soil arthropods are among the animals that inhabit those two environments. This study aims to look into the variety of soil arthropods in the ecosystem of Sungai Suci. Sampling bamboo and sea pine ecosystems was done by taking soil from the root areas of bamboo and sea pine. For each ecosystem, five soil samples are taken randomly as replication. The soil was placed in a smaller funnel and left for two weeks at room temperature 24ºC, and 12 hours of irradiation. Soil arthropods obtained from the three ecosystems were identified, and their numbers were recorded. The most common type of arthropod found in the three ecosystems was acarina. The study results showed that the diversity values of the bamboo and sea pine ecosystems, respectively, were 1.44 and 0.49; and. Dominance values for the bamboo and sea pine ecosystems, respectively, are 0.38 and 0.82. The evenness values of the bamboo and sea pine, respectively, are 0.56 and 0.18.
Co-Authors Agustin Zarkani Aulia, Ewa Djamilah Djamilah, Djamilah Fauzi, Ariffatchur Ifebri, Rihan Mimi Sutrawati Putra, Apriza Hongko Putri, Elsa Lolita Wibowo, Hariz Eko