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All Journal Agrikultura AGRITROPICA : Journal of Agricultural Sciences
Aulia, Ewa
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Agriculture, Biological Sciences & Forestry

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Deteksi dan Identifikasi Begomovirus pada Tembakau yang Ditanam Tumpang Sari dengan Tanaman Cabai Sutrawati, Mimi; Nadrawati, Nadrawati; Djamilah, Djamilah; Aulia, Ewa
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.47941

Abstract

Beragam spesies Begomovirus telah dilaporkan di berbagai daerah di Indonesia. Gejala penyakit mosaik menguning akibat infeksi Begomovirus telah dilaporkan pada berbagai tanaman budidaya di Bengkulu antara lain cabai, mentimun, labu, melon, pepaya, dan gulma. Tanaman tembakau banyak ditanam sebagai tanaman pinggir di lahan budidaya cabai. Berdasarkan pengamatan di lahan budidaya cabai di Kabupaten Kepahiang, Bengkulu ditemukan tembakau bergejala keriting dan mosaik kuning. Tujuan penelitian ini adalah mendeteksi dan mengidentifikasi Begomovirus penyebab penyakit mosaik menguning tembakau di Bengkulu. Deteksi dan identifikasi virus dilakukan dengan menggunakan metode polymerase chain reaction (PCR) menggunakan primer universal yang mengkode transcriptional activator protein (TrAp) dan replication-associated protein (Rep) dengan target fragmen ± 900 pb. Hasil amplifikasi PCR menunjukkan adanya pita target ± 900 pb pada sampel daun tembakau. Produk PCR kemudian dikirim ke First Base, Malaysia untuk dilakukan sikuensing. Begomovirus yang menginfeksi tembakau  menunjukkan homologi 99% dengan isolat Pepper Yellow Leaf Curl Indonesia Virus (PYLCIV) pada cabai di Bali (nomor aksesi LC381263). Hasil identifikasi ini merupakan laporan pertama infeksi PYLCV pada tanaman tembakau di Bengkulu.
Virus Identification in Yard Long Bean Plants with Yellow Mosaic Symptoms Sutrawati, Mimi; Aulia, Ewa
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 2 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.2.116-121

Abstract

Begomovirus infection, which causes yellow leaf curl disease, is commonly found in the Bengkulu province, affecting various plants, including chilli, melon, cucumber, papaya, and weeds. The viruses reported to date in long bean plants include Mungbean yellow mosaic India virus (MYMIV) and Bean common mosaic virus (BCMV). This study aimed to detect viruses in long bean samples collected from Musi Rawas (South Sumatra) and North Bengkulu (Bengkulu), areas where yellow mosaic symptoms resembling those caused by Begomovirus infection were observed. Virus detection was performed using PCR-based DNA analysis with specific MYMIV (MY1/MY2) primers, targeting a DNA fragment of approximately 238 bp. The results revealed that yard-long bean plants from Musi Rawas were positively infected with MYMIV, while those from North Bengkulu tested negative for MYMIV. Begomovirus or other viral infections may cause the yellow mosaic symptoms observed in North Bengkulu yard long beans. Therefore, further investigations using additional primers are needed to identify the specific virus responsible for the symptoms in this region.
Genetic Diversity of Papaya Using Molecular Markers Random Amplified Polymorphic DNA Aulia, Ewa; Sutrawati, Mimi; Simarmata, Marulak
AGRITROPICA : Journal of Agricultural Sciences Vol. 7 No. 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.7.1.17-22

Abstract

Papaya is one type of fruit that is widely consumed and cultivated by the farmer. However, genetic analysis has not been carried out on various types of papaya available on the market. This aims to determine the genetic diversity of papaya plants that can become genetic resources to fullfil food needs and genetic resources for breeders. Genetic analysis was conducted by Random Amplified Polymorphic DNA (RAPD) method using 11 papaya varieties consisting of Calina, Bangkok, Red Pomegranate, Sunrise, Orange Lady, Red Lady, Taiwan, Arum, Miba, Golden, and Local and using 15 RAPD primers consisting of OPA-1, OPA-2, OPA-8, OPA-16, OPC-4, OPC-11, OPC-13, OPC-20, OPD-20, OPE-2, OPE-6, OPE-11, OPE-14, OPM-6, and OPY-15. PCR-RAPD results were translated into binary data and then cluster analysis was conducted using the Unweighted Pair-Group Method Arithmetic (UPGMA) method using the Numerical Taxonomy and Multivariate System (NTSYS) program. The PCR-RAPD results of 11 papaya varieties successfully amplified 8 out of 15 primers that formed 112 DNA bands with 85 polymorphic bands. Genetic diversity analysis showed the results at 90% similarity coefficient formed 9 groups. Group 1 consists of Bangkok and Sunrise varieties. Group 2 consists of Red Pomegranate and Arum varieties. Groups 3 to 9 consisted of Calina, Miba, Local, Golden, Orange Lady, Red Lady, and Taiwan varieties, respectively.
Co-Authors Djamilah Djamilah, Djamilah Marulak Simarmata Mimi Sutrawati Nadrawati, Nadrawati