Djamilah Djamilah, Djamilah
DEpartment of Plant Protection of Agricultural Faculty, the University of Bengkulu

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Deteksi dan Identifikasi Begomovirus pada Tembakau yang Ditanam Tumpang Sari dengan Tanaman Cabai Sutrawati, Mimi; Nadrawati, Nadrawati; Djamilah, Djamilah; Aulia, Ewa
Agrikultura Vol 34, No 3 (2023): Desember, 2023
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v34i3.47941

Abstract

Beragam spesies Begomovirus telah dilaporkan di berbagai daerah di Indonesia. Gejala penyakit mosaik menguning akibat infeksi Begomovirus telah dilaporkan pada berbagai tanaman budidaya di Bengkulu antara lain cabai, mentimun, labu, melon, pepaya, dan gulma. Tanaman tembakau banyak ditanam sebagai tanaman pinggir di lahan budidaya cabai. Berdasarkan pengamatan di lahan budidaya cabai di Kabupaten Kepahiang, Bengkulu ditemukan tembakau bergejala keriting dan mosaik kuning. Tujuan penelitian ini adalah mendeteksi dan mengidentifikasi Begomovirus penyebab penyakit mosaik menguning tembakau di Bengkulu. Deteksi dan identifikasi virus dilakukan dengan menggunakan metode polymerase chain reaction (PCR) menggunakan primer universal yang mengkode transcriptional activator protein (TrAp) dan replication-associated protein (Rep) dengan target fragmen ± 900 pb. Hasil amplifikasi PCR menunjukkan adanya pita target ± 900 pb pada sampel daun tembakau. Produk PCR kemudian dikirim ke First Base, Malaysia untuk dilakukan sikuensing. Begomovirus yang menginfeksi tembakau  menunjukkan homologi 99% dengan isolat Pepper Yellow Leaf Curl Indonesia Virus (PYLCIV) pada cabai di Bali (nomor aksesi LC381263). Hasil identifikasi ini merupakan laporan pertama infeksi PYLCV pada tanaman tembakau di Bengkulu.
Pertumbuhan dan Hasil 9 Galur Harapan Kedelai (Glycine max (L.) Merrill) pada Lahan Berpasir Syaputra, Iwan; Suryati, Dotti; Djamilah, Djamilah
Akta Agrosia Vol 20 No 1 (2017)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.678 KB)

Abstract

This research was conducted in Beringin Raya Sungai Hitam Village, Muara Bangkahulu Subdistrict, Bengkulu City from January to April 2016.  The research aims to compare the growth and yield of 9 lines of soybean expectation with 2 varieties of comparison (Tanggamus and Demas 1) on sandy land. The design used in this study was Completely Randomized Block Design (RAKL) using a single treatment factor of 11 soybean genotypes consisting of 9 lines of expectation (G4AB, G2BB, G1DB, 13 ED, 14 DD, 19 BE, 25 EC, G511H / Anj-2-10, and G511H / Anj-1-3) and 2 varieties of comparison (Tanggamus and Demas 1), each treatment was repeated four times to obtain 44 experimental units. Variables that significantly affect the F test at 5% level, followed by cluster analysis of Scott-Knott at 5% level. Of the 9 lines of expectation tested only 13 ED lines, 14 DD, and 25 EC had a number of comparable books comparable to the comparison varieties (Tanggamus and Demas 1). The comparative varieties had moderate flowering, deep harvest age, highest plant height, number of productive branches and the largest number of fertile books compared to the 9 tested lines. Density 13 ED, 14 DD, 25 EC has a number of pods of content comparable to the comparison varieties. The G511H / Anj-2-10 strain has more number of seeds per pod than the comparable strain / variety tested. The ED 13 line has a seed weight per plot proportional to the comparison varieties (Tanggamus and Demas 1), but is higher than the expected line of test. While the 19 BE strain has a weight of 100 seeds higher than the other strains / varieties tested
Effect of Noni Fruit Extract (Morinda citrifolia L.) And Application Time To Control Crocidolomia binotalis Zell. On Cabbage Plant Rahmawati, Dewi; Djamilah, Djamilah; Simanihuruk, Bilman W
Akta Agrosia Vol 22 No 1 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1075.898 KB)

Abstract

Crocidolomia binotalis Zell is an important pest in Brassicaceae vegetable crops. Vegetable insecticide is one potential alternative to controlling the pest. This study aims to determine the application time of noni fruit extract and to determine the effective concentration of noni fruit extract as a vegetable insecticide in controlling C. binotalis larva on cabbage plants in the field. this study used a Completely Randomized Design (RAL) consisting of two treatment factors and four replications. The first factor is the concentration of extract (5 ppm, 10 ppm, 15 ppm and 20 ppm) and the second factor is the time of application of vegetable pesticide before the pest is invested (W1) and after the pest is invested (W2). So there are 32 units of experiments and 4 units of experiments added as control. The results showed, noni fruit extract had no significant effect on mortality of C. binotalis larvae. The highest mortality rate is at 100% K2 (10ppm) concentration at the time of application before the pest is invested (W1). The interaction had significant effect on the wet weight of the plant canopy but had no significant effect on the mortality of the pest, the intensity of the attack, the percentage of pupa formed, and the present percentage of imago.
Vegetable Insecticide as an Environmentally Friendly Alternative to Control Crocidolomia pavonana F. on Pak Choi Plants Djamilah, Djamilah; Fauzi, Ariffatchur; Ginting , Sempurna; Sutrawati , Mimi
AGRITROPICA : Journal of Agricultural Sciences Vol. 8 No. 1 (2025)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.8.1.43-47

Abstract

Pak choi plant (Brassica rapa L) is a type of vegetable that is popular and loved by the public. This cultivation cannot be separated from pest attacks. Crocidolomia pavonana F. (crop caterpillar) is an important pest that causes severe damage to Pak choi plants. Pest control with the application of synthetic chemical insecticides causes resistance to crop caterpillars, kills natural enemies of insect pests and hurts the environment and human health. Another environmentally friendly alternative for controlling C. pavonana is needed.  It is necessary to use vegetable insecticides. Jengkol skin and kabau skin have the potential as plant-based insecticides to control C. pavonana. This research aims to find concentrations of jengkol and kabau skin extracts (vegetable insecticides) that are effective in preventing C. pavonana on Pak choi plants. This research used a Completely Randomized Design (CRD). It was carried out in two stages, namely testing the effectiveness of jengkol and kabau skin extracts on C. pavonana in the laboratory. Laboratory tests were carried out with three treatments of jengkol and kabau skin extract concentrations at concentrations of 4%, 6% and 8% respectively, repeated five times. Observations were carried out for a week. The variable observed was larval mortality. The laboratory test research results showed that 66% mortality occurred in kabau skin extract at a concentration of 8%, and in jengkol skin extract at a concentration of 8%, causing 53% mortality of crop caterpillar larvae.
Diversity of Land Arthropods in Community Oil Palm Plantations in the Coastal Area of Bengkulu Fauzi, Ariffatchur; Zarkani, Agustin; Djamilah, Djamilah; Gustian, Deri; Hamidi, Ilmi; Ifebri, Rihan; Prastio, Turko
AGRITROPICA : Journal of Agricultural Sciences Vol. 8 No. 1 (2025)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.8.1.33-42

Abstract

Bengkulu is one of the provinces located on the island of Sumatra, boasting a significant agricultural sector. One of the leading agrarian commodities in Bengkulu Province is oil palm. Oil palm in Bengkulu is planted in coastal areas. One of the key factors supporting oil palm fruit production is a healthy soil arthropod ecosystem. Soil arthropods play an essential role in the oil palm plantation ecosystem. As organisms that live in the soil, these animals help maintain soil and plant health, improving soil structure and nutrient availability for oil palm plants. This study aims to obtain initial data on the diversity of soil arthropods and their roles in smallholder oil palm plantations on the coast of Bengkulu. This study began with sampling oil palm plantations around the coast of Bengkulu. The areas that will be used as study locations include Sungai Suci, Danau Gedang, and Pekik Nyaring. Arthropod sampling was carried out by installing pitfall traps in both areas, which were determined diagonally in a ​​200 m² area, with 20 pitfall trap units per location. Arthropod species were identified based on morphology, and the type and number of populations were recorded. The Shannon-Wiener diversity index, dominance index, and evenness index measure analysis of biodiversity data. The results of the study showed that in the majority, the diversity of soil arthropods in the three sampling areas was moderate, the dominance of arthropod types was low to moderate, and evenness was moderate to high.
Laporan Pertama Meloidogyne incognita yang Menginfeksi Tanaman Melon (Cucumis melo L.) pada Media Cocopeat di Bengkulu, Indonesia Hamidi, Ilmi; Fauzi, Ariffatchur; Djamilah, Djamilah; Zarkani, Agustin; Prastio, Turko
AGRITROPICA : Journal of Agricultural Sciences Vol. 8 No. 2 (2025)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.8.2.95-99

Abstract

Cocopeat, a growing medium derived from coconut husk fibers, is valued for its high porosity, strong water retention, and support for healthy root development. However, its physical properties may also provide favorable conditions for plant-parasitic nematodes. This study reports, for the first time, the occurrence of Meloidogyne incognita in melon (Cucumis melo L.) cultivated in cocopeat in Bengkulu, Indonesia, and examines the susceptibility of this medium to infestation. Root samples were purposively collected from six infected plants showing stunted growth, wilting, and root galling. Adult female nematodes were extracted and identified morphologically through perineal pattern analysis. The diagnostic features, such as a tall, narrow dorsal arch, fine striae, and the absence of lateral lines, consistently matched those of M. incognita. Galls of varying sizes were observed in all samples, indicating different infection intensities. This finding suggests that the physical structure of cocopeat may facilitate nematode mobility and persistence across growth stages. The study highlights that cocopeat is not inherently nematode-free despite its agronomic advantages and should be managed through preventive strategies, including substrate sterilization, sanitation, and regular nematode monitoring.