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All Journal Jurnal Biologi Tropis Health Research Journal of Indonesia JOURNAL OF Pharmacy and Tropical Issues
Matias Nataniel Kolobani
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Metabolite Profile of Boiled Ndusuk Leaves (Melastoma malabathricum L.) from Manggarai Based on GC-MS Analysis Bernadeta Stien Julian Nabuasa; Muhajirin Dean; Matias Nataniel Kolobani; Yantus Aristarkus B. Neolaka
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12888

Abstract

Ndusuk leaves (Melastoma malabathricum L.) are traditionally used by the Manggarai community to reduce the fatty appearance of pork dishes, yet the molecular basis of this practice has not been investigated using a GC-MS-based metabolomic approach. This study compared the metabolite profiles of Ndusuk leaf decoctions prepared without and with pork. Dried leaves (35 g) were decocted in 2.5 L of water at 100°C for 30 min under two treatments: leaves only (control) and leaves with 100 g pork (sample), each performed in triplicate (n = 3). Metabolites were analyzed by GC-MS and tentatively identified using the NIST library (similarity index ≥80%). The control contained 118 tentatively identified metabolites, whereas the pork-treated extract contained 126. Boiling with pork increased cis-vaccenic acid (+4.07%) and palmitic acid (+1.92%), while decreasing melezitose (−2.80%) and glycerol (−0.73%). These findings demonstrate that cooking with pork alters the metabolite profile of Ndusuk leaf decoctions without providing evidence of direct fat reduction. The observed metabolite shifts may suggest possible phenolic–lipid interactions, antioxidant-related processes, and Maillard reaction-associated changes; however, these mechanisms require confirmation using targeted metabolomics and authentic standards. This study provides the first metabolomic evidence supporting the traditional culinary use of Ndusuk leaves and establishes a scientific basis for Manggarai pork-cooking practices.
Profil kromatogram ekstrak etanol 95% dan etil asetat jahe merah (Zingiber officinale var. rubrum) Fitrya Rahmawaty Saleh; Muntasir Muntasir; Matias Nataniel Kolobani; Antonius Rolling Basa Ola
JOURNAL OF Pharmacy and Tropical Issues Vol. 6 No. 4 (2026): August edition 2026
Publisher : Indonesian Public Health-Observer Information Forum (IPHORR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/pti.v6i4.3962

Abstract

Background: Red ginger (Zingiber officinale var. Rubrum) is rich in bioactive compounds, such as gingerol and shogaol, which are highly sensitive to type of extraction solvent. Therefore, a chromatogram profile analysis is required to determine most effective solvent. Purpose: This study aims to compare chromatogram profiles of 95% ethanol and ethyl acetate extracts of red ginger rhizome using High-Performance Liquid Chromatography (HPLC). Method: This descriptive laboratory study extracted 90 gr of red ginger crude drug (simplisia) powder from Ende Regency, Flores, via maceration using 95% ethanol and ethyl acetate for 3 × 24 hours. Resulting filtrates were evaporated to obtain thick extracts, which were then analyzed using HPLC to evaluate the number of peaks, retention time, and peak area. Results: 95% ethanol extract produced a more complex chromatogram profile with 30 peaks, whereas the ethyl acetate extract yielded 22 peaks. This difference indicates superiority of 95% ethanol in extracting secondary metabolite compounds across a wider polarity range compared to ethyl acetate. Conclusion: There is a significant difference in chromatogram profiles between two extracts. 95% ethanol solvent proved to be more effective in extracting bioactive compounds from red ginger rhizome than ethyl acetate, serving as a baseline for herbal raw material standardization and a foundation for future research.   Keywords: HPLC; Red ginger; Zingiber officinale var. Rubrum.
Penetapan kadar rutin dan uji aktivitas antioksidan ekstrak etanol daun faloak (Sterculia quadrifida R.Br.) Ardila Nurjayastito Sulanjana Putri; Muntasir Muntasir; Matias Nataniel Kolobani; Muhajirin Dean
JOURNAL OF Pharmacy and Tropical Issues Vol. 6 No. 4 (2026): August edition 2026
Publisher : Indonesian Public Health-Observer Information Forum (IPHORR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/pti.v6i4.4004

Abstract

Background: Faloak (Sterculia quadrifida R.Br.) is a traditional medicinal plant used by people of East Nusa Tenggara that contains flavonoid compounds, one of which is rutin, which has potential as an antioxidant and anti-inflammatory. However, information regarding the distribution of rutin across various organs of Faloak plant and its antioxidant activity remains limited. Purpose: To determine rutin content in ethanol extract of Faloak (Sterculia quadrifida R.Br.) leaves using HPLC and to determine antioxidant activity of extract using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Method: This research is a descriptive study conducted in a laboratory by applying treatments to samples, including extraction using ethanol solvent and analysis of target compound using an HPLC instrument. Data generated consist of numerical values from measurement of rutin content and percentage inhibition of DPPH radicals in ethanol extract of Faloak leaves. Results: Calibration curve of rutin standard showed good linearity ( = 0.993). Ethanol extract of Faloak leaves contains rutin with an average content of 0.807 ± 0.094%. DPPH test results for Faloak leaf extract showed an  value of 102.17 ppm, which is classified as a moderate antioxidant category. Conclusion: Rutin was detected in quantifiable amounts in Faloak leaves. Faloak leaf extract also exhibited moderate antioxidant activity, consistent with detected rutin content, thereby demonstrating potential for development as a natural source of rutin with antioxidant activity.   Keywords: Antioxidant; DPPH; Faloak; HPLC; Rutin.
Analisis Faktor Yang Berhubungan Dengan Kepatuhan Pengobatan Pasien Hipertensi di Puskesmas Bakunase Kota Kupang Kristina Lanta; Magdarita Riwu; Nurul Fatmawati Pua Upa; Matias Nataniel Kolobani
Health Research Journal of Indonesia Vol 4 No 6 (2026): Health Research Journal of Indonesia
Publisher : CV. Wadah Publikasi Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63004/hrji.v4i6.1597

Abstract

Hipertensi merupakan penyakit kronis yang memerlukan pengobatan jangka panjang. Hasil Survei Kesehatan Indonesia 2023 menunjukkan prevalensi hipertensi di Nusa Tenggara Timur sebesar 28,2%, sedangkan data Dinas Kesehatan Kota Kupang 2023 mencapai 59,6%. Keberhasilan pengendalian hipertensi bergantung pada kepatuhan pengobatan, namun ketidakpatuhan masih terjadi dan meningkatkan risiko komplikasi. Penelitian ini bertujuan menganalisis faktor-faktor berhubungan dengan kepatuhan pengobatan pasien hipertensi di Puskesmas Bakunase Kota Kupang. Penelitian observasional analitik dengan rancangan cross-sectional ini menggunakan 92 responden melalui teknik consecutive sampling. Data dikumpulkan melalui wawancara menggunakan Kuesioner. Analisis dilakukan secara univariat, bivariat menggunakan uji Chi-Square, Fisher-Freeman-Halton Exact, Fisher Exact, dan multivariat menggunakan regresi logistik. Hasil penelitian menunjukkan bahwa mayoritas responden sebanyak 48 orang (52,2%) termasuk kategori patuh dan 44 orang (47,8%) termasuk kategori tidak patuh. Analisis bivariat menunjukkan bahwa komorbiditas, sikap, tingkat pengetahuan, dan motivasi diri bepengaruh signifikan terhadap kepatuhan pengobatan (p < 0,05). Setelah dilakukan analisis multivariat, hanya motivasi diri (OR = 16,6; 95% CI : 2,76 –99,71; p = 0,002) paling berhubungan signifikan dengan kepatuhan pengobatan pasien hipertensi. Oleh karena itu, diperlukan upaya peningkatan motivasi pasien melalui edukasi dan pendampingan berkelanjutan.
Kajian etnomedisin masyarakat desa Putri Yohana Roswita Lusi; Tadeus Andreas Lada Regaletha; Matias Nataniel Kolobani; Yendris Krisno Syamruth
JOURNAL OF Pharmacy and Tropical Issues Vol. 6 No. 3 (2026): July Edition 2026
Publisher : Indonesian Public Health-Observer Information Forum (IPHORR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/pti.v6i3.3673

Abstract

Background: Plant-based ethnomedicine practices remain a vital part of rural public health systems. Knowledge regarding the use of medicinal plants is passed down through generations, yet it has not been scientifically documented, placing it at risk of being lost as times change. Purpose: To identify the types of medicinal plants, the plant parts used, preparation methods, modes of administration, and the types of ailments treated by the village community. Method: This is a descriptive qualitative study employing an ethnographic approach. It was conducted in Camplong 2 Village, Fatuleu District, Kupang Regency, East Nusa Tenggara Province. Data were collected through observation, in-depth interviews, and documentation involving three key informants and 27 additional informants. Data analysis was performed through data reduction, data presentation, and conclusion drawing. Results: Forty seven species of medicinal plants utilized by the community were identified. The plant part most frequently used was the leaf (59%), the dominant preparation method was boiling (57%), and the most common method of administration was oral consumption (54%). These medicinal plants were used to treat 34 types of ailments categorized into 10 groups, with the category comprising fever and general bodily complaints being the most prevalent (19%). Conclusion: Village communities continue to maintain plant-based ethnomedicine practices rich in local knowledge. The diversity of medicinal plant species, along with the methods of their preparation and use, demonstrates that traditional medicine still plays a vital role in maintaining community health.   Keywords: Ethnomedicine; Medicinal Plants; Traditional Medicine.
Co-Authors Antonius Rolling Basa Ola Ardila Nurjayastito Sulanjana Putri Bernadeta Stien Julian Nabuasa Fitrya Rahmawaty Saleh Kristina Lanta Magdarita Riwu Muntasir, Muntasir Nurul Fatmawati Pua Upa Putri Yohana Roswita Lusi Tadeus Andreas Lada Regaletha Yantus Aristarkus B. Neolaka YENDRIS K SYAMRUTH