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Giyanto, dan

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Author-ID : 1119810

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Isolasi Rhizobakteri dan Pengaruh Aplikasinya dengan Pupuk N-P terhadap Mutu Benih dan Pertumbuhan Bibit Tetua Betina Jagung Asih, Pitri Ratna; Surahman, Memen; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Increased productivity of maize can be done with the use of high quality seeds from improved varieties such as hybrid seed. The objectives of this study were Increasing productivity of maize female parent is important in order to reduce the price of hybrid seed. The objectives of this study were to determine the nitrogen fixing bacteria compatible with phosphate solubilizing bacteria (PSB), and using those bacteria to increase physiological seed quality and seedling growth of maize female parent. The research consisted of laboratory and field experiments. Laboratory experiment for the isolation and identification of rhizobacteria resulted in 25 Azotobacter and 29 Actinomycetes non-pathogenic isolates capable of fixing nitrogen and PSB selected for compatibility tests were AB3, B28, P12, P14, P24, and P31. The compatibility test showed 25 pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes were mutually compatible. The BPF pair with Azotobacter or Actinomycetes P24-AzL7, P24-AzL9, B28-AcCKB4, P24-AcCKB9, P24-AcCKB20, and P24-AcCKW5 were able to increase the vigor index of hybrid maize female parent seed. Field experiment was arranged in a split plot design with three replications. The main plot was dosage of N-P fertilizer (0%, 25%, 50%, 75%, and 100% of recommendation dosage), and the subplot was 12 rhizobacteria treatments selected from 25 compatible pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes and 1 control. The application of compatible pairs of bacteria had a significant effect on plant height, the number of leaves at 3 and 4 weeks after planting and plant dry weight. However, the best treatment i.e. B28-AcCKB4 was not significantly different with the nutrient broth treatment (as control).

Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.) Tefa, Anna; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period

Uji Ketahanan Anggrek Hibrida Phalaenopsis terhadap Penyakit Busuk Lunak yang Disebabkan oleh Dickeya dadantii Firgiyanto, Refa; Aziz, Sandra Arifin; Sukma, Dewi; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTOne of the most popular orchids and cultivated by Indonesia and other countries is Phalaenopsis. The main disease of Phalaenopsis orchids in Indonesia is soft rot caused by bacteria Dickeya dadantii. The purpose of this study was to know the resistanctcy of Phalaenopsis hybrid to soft rot disease. The experiment was conducted at the Bacterial Plant Laboratory, Department of Plant Protection, the Greenhouse of Leuwikopo, Bogor Agricultural University, and the plastic house of Alam Sinar Sari Dramaga, Bogor from June 2014 to February 2015. The experimental design was randomized block design with three replications. Five genotypes of hybrid Phalaenopsis were tested, namely Phal. KHM 205, Phal. KHM 1126, Phal. KHM 1318, Phal. AMP 17, and Phal. KHM 2249. Phal. amabilis, Phal. esmeralda, Phal. amboinensis, and Phal. cornu-cervi were used as controls. The resistance testing was performed by inoculating bacteria D. dadantii on leaves of the orchids. The results showed all Phalaenopsis hybrid showed disease symptoms after inoculation. Phal. KHM 2249 had the lowest number of fallen leaves and the highest number of survive plants compared to the other hybrid Phalaenopsis. Survival rate was likely related to peroxidase activity and leaf thickness.Keywords: fallen leaves, leaf thickness, peroxidase, Phalaenopsis

Aplikasi Bakteri Probiotik Pseudomonas Kelompok Fluorescens untuk Meningkatkan Produksi dan Mutu Benih Cabai Permatasari, Okti Syah Isyani; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe use of high quality seed is one of the key factors to improve productivity. Probiotic bacteria has been used to increase plant growth and to control pathogens. The objective of the research was to evaluate methods of chili seed production that yielded high physiological and pathological quality using probiotic fluorescent bacteria Pseudomonas (P24). The bacteria was expected to function as plant growth promoting bacteria as well as capable of controling seedborne pathogen Colletotrichum acutatum causes antrachnose. The experiment was conducted during March until October 2014 in Seed Health Laboratory, Plant Bacteriology Laboratory, and Leuwikopo experimental garden, IPB. The experiment was arranged in a split plot randomized complete block design with three replications. The main plots were untreated and inoculation of C. acutatum. The subplot was six treatments of fluorescent Pseudomonas (P24) application. The results showed that matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery decreased disease incidence significantly. Matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery and flowering phase increased number of healthy fruit and total weight of seeds per plant significantly. These applications also increased seed physiological quality indicated by germination percentage (GP) 77.04%, growth rate (GR) 9.72% etmal-1, vigor index (VI) 29.74%, and seed health by suppresed C. acutatum infection up to 12.25%.Keywords: anthracnose, Colletotrichum acutatum, control, matriconditioning, spraying

Co-Authors Dewi Sukma Eny Widajati Memen Surahman Muhamad Syukur Permatasari, Okti Syah Isyani Pitri Ratna Asih Refa Firgiyanto Sandra Arifin Aziz Tefa, Anna

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