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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Natur Indonesia
Yuhendri, Vitriyanna Mutiara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Physics Social Sciences

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Distribution and Clinical Significance of Nuclear Factor Erythroid 2-Related Factor 2 Gene Polymorphism in Chronic Hepatitis B: A Cross-Sectional Study Yuhendri, Vitriyanna Mutiara; Labecka, Magda; Ibrahim, Sundus; Arfianti, Arfianti
Jurnal Natur Indonesia Vol. 23 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.23.1.10-15

Abstract

Chronic hepatitis B (CHB) infection is associated with serious complications, including liver cirrhosis and hepatocellular carcinoma (HCC). Nuclear factor erythroid 2-related factor 2 (NRF2) is a transcription factor that regulates the expression of antioxidant genes, helping protect cells and tissues from oxidative stress, a process involved in the pathogenesis of chronic hepatitis B (CHB). This study aimed to determine the distribution of NRF2 polymorphisms in CHB patients and their association with complications. The study included 68 CHB patients, with 33 having no complications and 35 with complications (Cirrhosis and HCC). Genotyping of the NRF2 polymorphisms, rs35652124 (A→G) and rs6721961 (C→A), was performed using confronting two-pair primers and polymerase chain reaction (PCR-CTPP). The serum levels of bilirubin, albumin, and alanine aminotransferase (ALT) were measured using commercial kits. The mean age of subjects was 45.34±1.32 years old on average. There was no significant difference in mean bilirubin and ALT levels between patients with and without CHB complications. However, patients without complications had significantly higher albumin levels than those with complications (4.0±0.8 vs. 3.37±0.7 g/dL; p<0.05). The most common genotypes for NRF2 rs35652124 were AG (51.85%), AA (40.74%), and GG (7.41%), while for NRF2 rs6721961, the were CA (51.47%), CC (45.59%), and AA (2.94%). The distribution of NRF2 genotypes did not differ significantly between CHB patients with and without complications (p>0.05). This study suggests that NRF2 gene polymorphisms may not contribute to the development of Cirrhosis and HCC in CHB. Further research with a larger sample size is needed to confirm these findings.
Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Yuhendri, Vitriyanna Mutiara; Pratiwi, Nurfi; Paulina, Ariza Julia; Ismawati, Ismawati; Saputra, Nicko Pisceski Kusika; Arfianti, Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Co-Authors Arfianti Arfianti Ariza Julia Paulina Ibrahim, Sundus Ismawati ismawati Labecka, Magda Nurfi Pratiwi, Nurfi Saputra, Nicko Pisceski Kusika