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The association of CAG repeat length polymorphisms of androgen receptor gene and spermatogenesis impairment in several Indonesian men Soeharso, Purnomo; Arfianti, Arfianti; Moeloek, Nukman
Medical Journal of Indonesia Vol 13, No 4 (2004): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.995 KB) | DOI: 10.13181/mji.v13i4.154

Abstract

Spermatogenesis impairment is the main cause of infertility in men. Androgen is believed to play a critical role in regulating spermatogenesis. Androgen acts by binding to the androgen receptor (AR) which is a protein regulator of DNA transcription. Exon 1 of AR gene contains a CAG repeat length polymorphism and it is believed to interfere AR function. This study includes DNA isolation from peripheral blood and amplification of CAG repeat fragments by PCR method. CAG repeat lengths were determined by electrophoresis on 6% denaturing gel polyacrylamide. We found that the mean CAG repeat lengths were 24,3 ± 3,4 in oligozoospermic/azoospermic men and 22,7 ± 2,7 in normozoospermic men. The difference in CAG repeat length between the two groups was statistically significant (p = 0,031, t-test). Nevertheless, there was no correlation between CAG repeat lengths and sperms concentration (rs = -0,038; p = 0,775). This result suggest that the expansion of CAG repeat length was not the main cause of spermatogenesis impairment. (Med J Indones 2004; 13: 215-20)Keywords: androgen receptor, CAG repeat, IVF/ICSI, male infertility.
BIOSENSOR SYSTEM DESIGN FOR DISSOLVED OXYGEN MEASUREMENT USING CURRENT MIRROR CIRCUIT Lazuardi Umar; Rahmondia N Setiadi; Arfianti; Tetty M Linda
Spektra: Jurnal Fisika dan Aplikasinya Vol 4 No 2 (2019): SPEKTRA: Jurnal Fisika dan Aplikasinya, Volume 4 Issue 2, August 2019
Publisher : Program Studi Fisika Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.982 KB) | DOI: 10.21009/SPEKTRA.042.05

Abstract

A biosensor system using a current mirror circuit has been designed and characterized. Biosensor system usually implements an amperometric circuit such as a trans-impedance topology to measure current flowing in the solution due to the contained dissolved oxygen. The trans-impedance circuit implemented for the biosensor has some drawbacks and disadvantages. The implementation of this circuit exhibits rather high noise, besides the impracticality of the power supply used due to bipolar voltage need to allow negative voltage output. In this paper, a classical current mirror circuit is used to convert the flowing current in the solution. The use of the classical current mirror circuit is aimed to obtain lower noise level, besides the easiness in providing the power supply for the system, since the circuit consists only of small number of active components and does not need a bipolar power supply. The measurement is performed utilizing an algae species as a bio-receptor for the measured dissolved oxygen. By utilizing this circuit, the biosensor measurement system can be optimized with better precision and noise performance. Consequently, the dissolved oxygen measurements can be improved and achieve good results. Besides, the system can detect the changes in dissolved oxygen due to the photosynthetic process of the algae.
Akurasi Indeks Antropometri Obesitas dalam Mempediksi Kontrol Glicemic Pasien Diabetes Mellitus Tipe 2 di Puskesmas Kota Pekanbaru Arfianti, Arfianti; Deyana, Frilly; Rosdiana, Dani; Ardini, Nadiah Yamanza; Armi, Wahyuli; Kesumah, Dewi Iramayana Sandra
Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n3.1361

Abstract

Diabetes melitus (DM) tipe 2 adalah penyakit metabolik yang ditandai dengan peningkatan kadar gula darah. Kontrol gula darah merupakan salah satu tujuan utama tata laksana pasien DM. Obesitas merupakan faktor risiko utama dari DM tipe 2. Tujuan penelitian ini adalah mengetahui hubungan indeks antropometri dengan kontrol gula darah pada pasien DM tipe 2 yang dirawat jalan di puskesmas Kota Pekanbaru. Desain penelitian yang digunakan adalah observasional analitik dengan pendekatan cross-sectional. Sampel pada penelitian ini meliputi 103 pasien DM tipe 2 yang diperoleh dari Puskesmas Sidomulyo dan Simpang Tiga Kota Pekanbaru pada bulan Mei–Juni 2018.Subjek penelitian paling banyak perempuan (58,3%) dan berusia ≥45 tahun (96,1%). Tingkat pendidikanterbanyak adalah SMA (42,7%) dan 54,4% tidak bekerja. Riwayat diabetes ditemukan pada 68,9% subjek dan 85,4% subjek tidak memiliki riwayat merokok. Sebanyak 98,1% pasien diabetes mendapat obat hipoglikemik oral dan 84,5% pasien diabetes memiliki kadar gula darah tidak terkontrol. Penelitian ini menunjukkan bahwa obesitas berdasar atas IMT dan WC tidak berhubungan dengan kontrol gula darah. Sebaliknya, obesitas berdasarkan WHR (p=0,014) dan WHtR (p=0,015) memiliki hubungan dengan kontrol gula darah pada pasien DM. Namun WHR dan WHtR secara tunggal memiliki akurasi yang sangat lemah dalam memprediksi kontrol gula darah pada penderita DM tipe 2.Accuracy of anthropometric Indexes of Obesity to Predict Glycemic Control among Type 2 Diabetic Patients in Primary Health Care in PekanbaruType 2 diabetes mellitus (DM) is a metabolic disease characterized by elevated blood sugar levels. Glycemic control is the main goal of DM patient management. Obesity is a major risk factor for DM. This study aimed to investigate the association between anthropometric indexes and glycemic control in DM patients visiting primary health care facilities in Pekanbaru. This was a cross-sectional analytical observational study on 103 type 2 DM patients from Sidomulyo and Simpang Tiga public health centers in Pekanbaru during the period of May-June 2018. More female subjects participated in this study (58.3%) with overall average age of ≥45 years (96.1%). The highest level of education of the subjects was senior high school (42.7%) and most subjects were unemployed (54.4%). The family history of DM was found in 68.9% subjects and majority had no smoking history (86.4%). As many as 98.1% DM patients were taking oral hypoglicemic drugs. Most of the DM patients had uncontrolled glycemic status (84.5%). This study showed that BMI-based and WC-based obesity was not associated with glycemic control in DM patients. On the other hand, the association between obesity based on WHR (P = 0.02; OR = 5.26) and WHtR (P = 0.008; OR = 6.82) and glycemic control in DM patients was statistically significant. However, WHR dan WHtR alone has no discrimination power to predict glycemic control among type 2 diabetic patients.
Distribusi Genotipe dan Subtipe Virus Hepatitis B pada Penderita Hepatitis B Kronik di Pekanbaru Arfianti, -; Zainal, Andi; Andrini, Fauzia; Endriani, Rita
Majalah Kedokteran Bandung Vol 43, No 3
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Variasi genetik virus hepatitis B (VHB) dapat mempengaruhi manifestasi klinis, risiko karsinoma hepatoselular (KH), dan respons terhadap terapi antiviral. Desain penelitian adalah analitik cross-sectional yang bertujuan untuk mengetahui apakah terdapat perbedaan distribusi genotipe dan subtipe VHB pada berbagai manifestasi klinis hepatitis B kronik di Pekanbaru. Sebanyak lima puluh dua serum penderita hepatitis B kronik di Pekanbaru diperoleh sejak bulan Maret sampai Agustus 2009 telah diperiksa pada penelitian ini. Analisis statistik menggunakan uji Fisher. Penentuan genotipe VHB dilakukan dengan membandingkan sikuens gen S pada penelitian ini dengan sikuens gen S yang telah dipublikasi pada GenBank. Hasil penelitian menunjukkan bahwa 31 (60%) penderita terinfeksi oleh genotipe C dan 21 (40%) oleh genotipe B. Berdasarkan manifestasi klinis, genotipe C merupakan genotipe yang paling banyak ditemukan pada donor darah (7/10 penderita) dan sirosis (7/10 penderita), sedangkan pada penderita hepatitis B kronik aktif dan KH ditemukan frekuensi genotipe B dan C yang hampir sama. Subtipe adr (60%) merupakan subtipe yang paling banyak ditemukan, diikuti oleh subtipe adw (38%) dan ayw (2%). Berdasarkan hasil uji stastitik tidak ditemukan perbedaan bermakna distribusi genotipe dan subtipe pada berbagai manifestasi klinis infeksi VHB. Namun, genotipe C (8/9 penderita) merupakan genotipe VHB yang paling banyak ditemukan pada penderita KH dengan sirosis, sedangkan genotipe B (8/11 penderita) merupakan genotipe VHB yang paling banyak menginfeksi penderita KH tanpa sirosis (p=0,01). Simpulan, genotipe VHB mungkin berhubungan dengan patogenesis karsinoma hepatoselular dengan sirosis dan tanpa sirosis. [MKB. 2011;43(3):105–11].Kata kunci: Genotipe, hepatitis B kronik, karsinoma hepatoselular, sirosis, subtipe Distribution of Hepatitis B Virus Genotypes and Subtypes Among Chronic Hepatitis B Patients in PekanbaruGenetic variance of hepatitis B virus (HBV) may influence the clinical manifestation, development of hepatocellular carcinoma (HCC), and response to antiviral treatment. This was analytic cross-sectional study which aimed to investigate the distribution of HBV genotypes and subtypes among different clinical status of chronic hepatitis B in Pekanbaru. A total of fifty-two of sera from chronic hepatitis B patients in Pekanbaru was collected from March to August 2009. Statistical analysis was performed by using Fisher test. HBV genotypes were examined based on homology of S gene from this study with those of GenBank database. Of these subjects, 31 (60%) and 21 (40%) were infected by genotype C and B, respectively. Based on clinical manifestation, genotype C was predominantly found among HBsAg-positive blood donors (7/10 patients) and liver cirrhosis (7/10 patients), whereas genotype B and C were comparable among chronic hepatitis B active and hepatocellular carcinoma patients. Subtype adr (60%) was the most prevalent, followed by subtype adw (38%) and subtype ayw (2%). There were no significant difference in the distribution of HBV genotypes and subtypes among diverse clinical manifestation of HBV infection. However, genotype C was predominantly detected among cirrhotic-HCC (8/9 patients) , while genotype B was mostly identified among non cirrhotic-HCC (8/11 patients) (p=0.01). In conclusion, HBV genotype may be associated with the pathogenesis of cirrhotic and noncirrhotic-HCC. [MKB. 2011;43(3):105–11].Key words: Chronic hepatitis B, genotype, hepatocellular carcinoma, liver cirrhosis, subtype DOI: http://dx.doi.org/10.15395/mkb.v43n3.54
FAKTOR RISIKO HEPATITIS B PADA TENAGA KESEHATAN KOTA PEKANBARU Amtarina, Rina; Arfianti, A; Zainal, Andi; Chandra, Fifia
Majalah Kedokteran Bandung Vol 41, No 3
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Penyakit hepatitis B tergolong penyakit yang menjadi masalah kesehatan serius di Indonesia. Penularan virus hepatitis B melalui kontak dengan produk darah. Data mengenai pembawa HBsAg dan faktor risiko penularan VHB di Kota Pekanbaru belum ada sehingga perlu diadakan penelitian mengenai hal tersebut. Penelitian dilakukan secara retrospektif terhadap hasil pemeriksaan HBsAg dan anti-HBs responden dengan cara menganalisis hasil jawaban kuesioner. Responden dibagi 2 kriteria yaitu kriteria pernah/sedang terpapar hepatitis B dengan hasil pemeriksaan anti-HBs positif HBsAg negatif atau HBsAg positif Anti-HBs negatif dan kriteria tidak pernah terpapar VHB dengan hasil pemeriksaan HBsAg dan anti-HBs negatif. Dari 110 responden, 32 orang (29,1%) hasil anti-HBs positif, HBsAg negatif, 1 orang (0,9%) hasil HBsAg positif, anti-HBs negatif. Dari analisis kuesioner, faktor risiko penularan dari 32 orang anti-HBs positif terbanyak melalui pernah cabut gigi yaitu sebanyak 29 orang (90,6%) diikuti dengan pernah tertusuk jarum bekas/tidak steril sebanyak 18 orang (56,2%). Hanya 3 (9,3%) dari 32 orang pernah menderita hepatitis B sebelumnya. Pada 1 orang dengan HBsAg positif, faktor risiko penularan melalui tertusuk jarum bekas/tidak steril, pengobatan akupuntur, cabut gigi, dan ada anggota keluarga serumah yang pernah menderita hepatitis B. Faktor risiko penularan terbanyak pada tenaga kesehatan di Pekanbaru adalah melalui cabut gigi dan tertusuk jarum bekas/tidak steril.Kata kunci: Hepatitis B virus, anti-HBs, HBsAg, tenaga kesehatan, faktor risikoRISK FACTORS FOR TRANSMISSION OF HEPATITIS B VIRUS INFECTION IN HEALTH CARE WORKER OF PEKANBARU CITYHepatitis B is still the one of serious public health problem in the world including Indonesia. Transmission of hepatitis B virus (HBV) is strongly associated with use of contaminated blood products. For many people infected with HBV, risk factors of transmission are unknown. We examined risk factors for acquiring HBV in health care worker. This research was done by retrospective to blood participants who tested positive for HBs antibody or HBs antigen, using a questionnaire.participants divided into 2 groups. Had/having exposed to VHB with ati-HBs Positive HBsag negative or HBsag positive, anti-HBs negative and never had exposed to VHB with HBsag and anti-HBs negative. Positive anti- HBs were identified in 32 (29.1%) of 110 participants. Positive HBs antigen anti-HBs negative was identified in 1 (0.9%) of 110 participants. In questionnaire analysis, significant risk factors for HBV infection among HBs antibody - positive participants were tooth extraction in 29 (90.6%), and needle stick injuries in 18 (56.2%) of 32 participants. Only 3 (9.3%) of 32 participants with HBs antibody - positive had history of post infected HBV for several years ago. In 1 participant with HBs antigen - positive, significant risk factors were needle stick injuries, acupuncture, tooth extraction, and contact infected person. The most significant risk factors for transmission of hepatitis B in health care worker in Pekanbaru city are tooth extraction and needle stick injuries.Key words: Hepatitis B virus (HBV), HBs antibody, HBs antigen, health care worker, risk factor DOI: http://dx.doi.org/10.15395/mkb.v41n3.245
Hubungan Kadar Vitamin D dan Gangguan Fungsi Hati pada Pasien Hepatitis B Kronik Hendri, Sherin Alifia; Arfianti, Arfianti
Jurnal Ilmu Kedokteran Vol 15, No 1 (2021): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26891/JIK.v15i1.2021.12-20

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Vitamin D plays an important role in the pathogenesis of chronic hepatitis B (CHB) through regulating immune response. This study aimed to determine the relationship between vitamin D levels and impaired liver function in CHB patients. This was an observational analytical study conducted from March to October 2019. We included 36 CHB patients aged 38.31±12.98 years and consisted of 16 men and 20 women. Vitamin D levels were measured by enzyme-linked immune assay (ELISA) method while albumin and bilirubin levels were examined by colorimetric assay. Vitamin D levels were in normal limits in 80.6% and insufficiency/deficiency in 19.4% of CHB patients. ALBI ratio scores were good in 75% and moderate-poor in 25% of subjects. Furthermore, there was no significant association between vitamin D levels and the ALBI ratio. In conclusion, this study shows that there is no relationship between plasma vitamin D levels and impaired liver function in CHB patients.
POLYMORPHISM OF INTERLEUKIN-10 (-819) GENE IS NOT ASSOCIATED WITH LIVER DAMAGE AMONG CHRONIC HEPATITIS B PATIENTS Ramadhani, Nur'aina ID; Arfianti, Arfianti
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 3, No 3 (2021): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v3i3.3340

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Introduction: Chronic hepatitis B (CHB) is characterized by chronic liver inflammation andhepatocellular damage caused by hepatitis B virus (HBV), leading to fatal liver diseases such as cirrhosis and hepatocellular carcinoma (HCC). Liver damage causes elevated levels of serum alanine aminotransferase (ALT). Previous studies have shown that IL-10 gene polymorphism (-819 C/T) is linked to several diseases, including inflammatory, autoimmune diseases, and cancer. Purpose: To investigate the relationship between IL-10 gene polymorphisms (-819 C/T) and liver damages in chronic hepatitis B patients at Arifin Achmad Regional Hospital, RiauProvince.Method: This study was an analytical study with a cross-sectional approach. Liver damages were examined by measuring ALT levels, and IL-10 gene polymorphisms were analyzed using Amplification-Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR). Results: There were 74 subjects included in this study, aged 45.47±13.42 years old,consisting of 43 males (58.1%) and 31 females (41.9%). Of 74 subjects, 35 CHB patientswere presented without complication, 26 CHB patients were complicated with cirrhosis, and 13 CHB patients were complicated with hepatoma. The genotype of the IL-10 (-819) gene polymorphism in this study was found as follows: CT genotype in 34 subjects (45.9%), TT genotype in 30 subjects (40.5%), and CC genotype in 10 subjects (13.5%). There was nosignificant relationship between IL-10 gene polymorphisms (- 819) and liver damage inchronic hepatitis B patients (p> 0.05).Conclusion: IL-10 gene polymorphism (-819) may not contribute to liver damages amongCHB patients.
Pengaruh Kelainan Refraksi terhadap Prestasi Belajar Murid Sekolah Dasar X Pekanbaru Yulia Wardany; Nurul Humairah Arfiza; Arfianti Arfianti
Jurnal Kesehatan Melayu Vol 1, No 2 (2018): April 2018
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.691 KB) | DOI: 10.26891/jkm.v1i2.2018.81-87

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Prestasi belajar dipengaruhi oleh faktor internal dan eksternal. Salah satu faktor internalnya adalah kesehatan mata. Anak yang mengalami kelainan refraksi berpotensi untuk mengganggu prestasi belajar. Penelitian ini bertujuan untuk mengetahui pengaruh kelainan refraksi terhadap prestasi belajar siswa SD X Pekanbaru. Design penelitian ini adalah penelitian analitik dengan pendekatan cross-sectional. Teknik pengambilan sampel menggunakan metode total sampling pada 555 murid. Pemeriksaan visus digunakan untuk mendeteksi kelainan refraksi sedangkan ranking di sekolah digunakan sebagai acuan prestasi belajar siswa. Prevalensi kelainan refraksi ditemukan pada 16%. Hasil uji statistik tidak memperlihatkan adanya perbedaan prestasi belajar pada anak dengan atau tanpa kelainan refraksi. Oleh karena itu, penelitian ini menyimpulkan tidak adanya pengaruh kelainan refraksi terhadap prestasi belajar pada murid sekolah dasar.
Faktor yang Memengaruhi Kesalahan Persepsi Ibu tentang Status Gizi Anak Arfianti Arfianti; Tri Muhti Puja Kesuma; Muhammad Beni Septima; Riefni Silara Dini; Fifia Chandra
Media Kesehatan Masyarakat Indonesia Vol. 15 No. 4: DESEMBER 2019
Publisher : Faculty of Public Health, Hasanuddin University, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.758 KB) | DOI: 10.30597/mkmi.v15i4.7461

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Malnutrition is a significant health problem among Indonesian children. Mothers play a very importantrole in the prevention of childhood malnutrition. This study aimed to compare the child’s nutritional statusbased on mothers’ perception and anthropometry measurement and to analyze contributing factors of maternalmisclassification of a child’s weight status. This was an analytical observational study with a cross-sectional design.Research subjects included 250 mothers of grade 4 and 5 students from 6 elementary schools in Pekanbaru cityrecruited by the proportional quota sampling method. The Nutritional status of children was classified accordingto the 2006 World Health Organization (WHO) anthropometry standard. Mothers’ perception of child’s weightstatus was collected using questionnaires. Based on anthropometry measurement, 28% and 10% of children wereoverweight and underweight, respectively. In contrast, according to mothers, 4,8% of children were overweightand 12,8% ofchildren were underweight. As many as 34.8% of mothers had incorrect perceptions about thenutritional status of their children and there was a poor agreement of mothers’ perception and child’s nutritionalstatus (Kappa coefficient of 0,02). Working mothers were more likely to underestimate their child’s weight statuscompared to stay-at-home mothers (p=0,004).
Faktor Risiko Obesitas dan Profil Lipid Darah pada Mahasiswa Baru Universitas Riau Elda Nazriati; Arfianti Arfianti; Ismawati Ismawati
Jurnal Ilmu Kedokteran Vol 4, No 2 (2010): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.022 KB) | DOI: 10.26891/JIK.v4i2.2010.123-132

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Obesity is a substansial public-health crisis in numerous developing country including Indonesia. Morbidity and mortalityin adult population is increased in individuals who were overweight in adolescence even if they lose the extra weightduring adulthood. Many factor influencing occurence of obesity, such as food pattern and activity pattern.The aims ofthis study was to describe risk factor of obesity of obese students. Samples were 2006’s obese students of RiauUniversity who had doing medical Check-up in Balai Kesehatan of Riau University. A descriptive study conducted on27 obese students in Riau University class 2006. variables of this study are risk factor of individual and family, foodpattern, and activity pattern. Obese criterian was taken by measured BMI. Food pattern and activity pattern was takenby using questionnaire. This study showed that 15 students (55,5 %) wereobese II (BMI > 30), 100 % students arecentral obesity, 59% students had mild consumtion risk factor and 22% students had high consumtion risk factor. 16% student had mild and 74 % student had high activity risk factor.