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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Media Penelitian dan Pengembangan Kesehatan
Saputra, Nicko Pisceski Kusika
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Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Medicine & Pharmacology Social Sciences

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ANALISIS FAKTOR RISIKO DISMENORE PRIMER DAN DISMENORE SEKUNDER PADA MAHASISWI Putra, Amrullah Syah; Saputra, Nicko Pisceski Kusika; Noviardi, Noviardi; Ismawati, Ismawati
Media Penelitian dan Pengembangan Kesehatan Vol. 34 No. 1 (2024): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v34i1.1974

Abstract

While the incidence of dysmenorrhea is relatively high worldwide, the prevalence of dysmenorrhea reported in the literature varies substantially. This study aimed to analyze the risk factors for primary dysmenorrhea and secondary dysmenorrhea. This research is a cross analytical design. The sample in this study was all medical faculty students at Riau University in 2019-2022, namely 410 people. From the research results, it was found that there was a relationship between menstrual blood volume, history of food allergies and stress with the incidence of primary dysmenorrhea. Meanwhile, the incidence of secondary dysmenorrhea was related to age, menstrual cycle, history of the sample's gestational age at birth, history of low birth weight (LBW), history of emotional violence, stress and birth history of samples from preeclamptic mothers at the time of delivery. This research concluded that there were several significant findings regarding factors related to the occurrence of dysmenorrhea in Riau University medical students in 2019 - 2022. The research showed the importance of paying attention to factors such as the length of the menstrual cycle, history of food allergies, history of emotional violence, stress, and history of maternal preeclampsia in efforts to prevent and manage dysmenorrhea in the population. It is recommended that future research further explore the association between the identified risk factors and the incidence of dysmenorrhea, potentially revealing additional insights to guide clinical practice and public health initiatives.
Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Yuhendri, Vitriyanna Mutiara; Pratiwi, Nurfi; Paulina, Ariza Julia; Ismawati, Ismawati; Saputra, Nicko Pisceski Kusika; Arfianti, Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Co-Authors Arfianti Arfianti Ariza Julia Paulina Ismawati ismawati Noviardi Noviardi, Noviardi Nurfi Pratiwi, Nurfi Putra, Amrullah Syah Yuhendri, Vitriyanna Mutiara