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All Journal Jurnal Biologi Tropis Jurnal Laot Ilmu Kelautan
Yusmalinda, Ni Luh Astria
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Immunology & microbiology

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Molecular Identification of Wedgefishes Stingrays (Rynchobatus sp.) at PPI Rigaih, Aceh Jaya Regency by using COI Gene Amin, Waldi; Bahri, Samsul; Malik, Danie Al; Yusmalinda, Ni Luh Astria; Ningsih, Enex Yuniarti; Pertiwi, Ni Putu Dian; Sembiring, Andrianus
Jurnal Laot Ilmu Kelautan Vol 5, No 2 (2023): Jurnal Laot Ilmu Kelautan
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/jlik.v5i2.7655

Abstract

Indonesia is one of the largest shark and ray producing countries in the world. Based on FAO (Food and Agriculture Organization) data, Indonesia is the top country which produce plenty of sharks and rays every year. One of the stingray shark caught by the fishermen called ikan pari kekeh (kekeh stingray), data from the IUCN( International Union for Conservation of Nature and Natural Resources)  this ray is included in Red List and categorized in Appendix II CITES (Convention on international trade of endangered species) due to excessive fishing. In most cases, the identification of kekeh ray is not easy because the information and conservation data are very limited. This study was conducted to identify the species of kekeh stingray landed at Rigaih Fish Landing Port, Aceh Jaya Regency using the COI gene (Cytochrome c oxidase subunit I) molecular analysis.  Sampling was carried out in June 2021 and then analysed at Indonesian Biodiversity Foundation (BIONESIA) laboratory. The analysis results were adjusted to GenBank data via BLAST (Basic Local Alignment Search Tools). The results of the analysis found per value. The ident of 10 samples ranged around 99-100% each and the query qover value each sample is 100%. The farthest genetic distance value is 0.34. The phylogenetic tree managed to classify species from each sample and form a cluster together with the data obtained from the NCBI genbank
Genetic Diversity and Species Identification of Unhatched Sea Turtle Eggs from Southern Bali Hatcheries Dewana, I Gede Jose; Pertiwi, Ni Putu Dian; Yuliastuti, Yuliastuti; Yusmalinda, Ni Luh Astria; Luh Putu Candra Apriliani; Savitri, Ni Komang Rossa Sri; Rachmawati, Rita; Sembiring, Andrianus
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9897

Abstract

Genetic data of sea turtles in Southern Bali remains limited as most previous studies rely on adult or live samples, while unhatched eggs are rarely utilized as a genetic resource. This study aims to identify the species and genetic diversity of unhatched sea turtle eggs from conservation sites in Southern Bali using mitochondrial control region. A total of six non-viable eggs were analyzed through DNA extraction, PCR, sequencing, and data analysis using MEGA 11, NCBI BLAST, and DnaSP v6. The identification results revealed that all samples belonged to the species of Lepidochelys olivacea (olive ridley turtle), with sequence similarity ranging from 99.54% to 100% and query coverage of 89% to 99% compared to reference data from GenBank. Phylogenetic analysis showed that all samples clustered within the same clade, with intraspecific genetic distances ranging from 0 to 0.008. Genetic diversity analysis revealed a haplotype diversity (Hd) of 0.86667 and nucleotide diversity (π) of 0.00306, with a total of four distinct haplotypes and six polymorphic sites. These findings indicate that unhatched sea turtle eggs still contain viable genetic material for analysis and suggest that Lepidochelys olivacea is the dominant nesting species found in Southern Bali. This study carries important implications for advancing conservation policy, enhancing habitat protection, minimizing inbreeding risks, and strengthening the use of species identification through forensic genetics.
Co-Authors Amin, Waldi Andrianus Sembiring, Andrianus Dewana, I Gede Jose Luh Putu Candra Apriliani Malik, Danie Al Ni Putu Dian Pertiwi, Ni Putu Dian Ningsih, Enex Yuniarti Rita Rachmawati, Rita Savitri, Ni Komang Rossa Sri Veithzal Rivai Zainal Yuliastuti Yuliastuti