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Journal : Rumphius Pattimura Biological Journal

Three Behaviors of Apis cerana Fabricius in Response to Supplementary Feed of Pulp and Paste of Tongka Langit Banana Hartanto, Muchmin; Lamerkabel, Jacobus Suruka Aifaman; Mustapeng, Andi Maryani A; Riupassa, Pieter Agusthinus
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p069-077

Abstract

Apis cerana Fabricius is a honeybee commonly cultivated in Indonesia to produce honey. Additional feed can acts as a controller of honeybee colonies to protect them from migrate, in uncertain local weather conditions. Providing additional feed based on ‘tongka langit’ banana, Musa troglodytarum L. which have a high β-carotene nutritional content influences the adaptation, eating, and resting behaviors of Apis cerana. This study describes and analyses those three behaviors of Apis cerana to the provision of the feed. The study was conducted for 10 days with the provision of three treatment feeds, namely K+ (sugar water), P1 (fresh banana pulp), and P2 (banana paste) on the first day, and data collection was carried out the next day with a total of 5 times data collection on the number of individuals. Data represents for three types of behavior parameters (adaptation, eating, and resting) will be subjected to a two-way analysis of variance and continued with Duncan's test. Of the three treatments, result was found that P2 has the largest number of individuals in adaptation behavior, while in eating behavior it showed that K+ (as control) has the largest. Treatment K+ and P2 were also not significantly different, after Duncan’ comparison test. The number of individuals of resting behavior from P2 treatment observed on ninth day slightly increased to that of K+ treatment. This is reflected that P2 has potential as additional natural feed for Apis cerana, with high β-carotene content, then might inclusively turn to the honey, even in low levels.
Measurement of Nitrate Reductase Activity in Vivo Using Sepctrophotometry Method Mose, Windi; Dorlandt, Mauritius; Riupassa, Pieter Agusthinus; Wael, Syahran
RUMPHIUS Vol 7 No 1 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i1p025-030

Abstract

Spectrophotometry is a quantitative measurement technique of a compound based on the measurement of absorbance or light transmission passing through the compound. The spectrophotometric method can be used to analyze the content of compounds. One of the enzymes whose activity can be measured in vivo is the nitrate reductase enzyme. The nitrate reductase enzyme is an enzyme that plays a role in the synthesis of ammonium and amino acids. The results of measuring the activity of the nitrite reductase enzyme with spectrophotometry showed that the highest NRA value was found in the leaves of red chili plants and the lowest was in the sugarcane leaf sample. NRA can be used as a selection parameter to predict the results of a plant, and has the potential to be applied to seed plants.