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THE EFFECT OF SEVERAL TOOTHPAST ON THE GROWTH OF Streptococcus mutans Nawaly, Hermanus; Pagaya, Joseph; Kaihena, Martha
RUMPHIUS Vol 2 No 1 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i1p011-018

Abstract

This study aims to determine the effect of several toothpastes on the growth of Streptococcus mutans which is the main cause of tooth decay. The study used 5 types of toothpaste, namely toothpaste A, toothpaste B, toothpaste C, toothpaste D, and toothpaste E. The antibacterial testing method used the diffusion method with the cup-plate technique. The significance level of each type of toothpaste was measured using a completely randomized design followed by a Tukey test to see differences in toothpaste treatment. The results showed that toothpaste A with active ingredients Monofluorophosphate Sodium 1.12%, Triclosan 0.3%, Calcium Glycerophosphate 0.13% had the largest average resistance zone of 27.3 mm. Whereas Toothpaste E with the active ingredient 0.08% Sodium Monofluorophosphate, 0.01% Sodium Fluoride has the smallest average resistance zone, which is 14.25 mm. The diameter of the ANOVA test zone of several types of barrier toothpaste against Streptococcus mutans at 95% confidence intervals showed that there was a significant difference.
MENGENAL SISI LAIN POHON MAYANG BAGI KEHIDUPAN MANUSIA Kaihena, Martha; Nusaly, Windy Natalia; Patty, Kristi Lenci; Salamena, Fuadiska; Manurung, Brian Saputra
Balobe: Jurnal Pengabdian Masyarakat Vol 3 No 2 (2024): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.3.2.113-117

Abstract

The community service activity with the title "Knowing the Other Side of Mayang Tree for Human Life" is a form of concern for academics in the field of Biology for the spread of society and the development of home industry products that have an impact on improving the health and economy of the community. The transfer of information in the form of processing techniques, ways of utilisation, manufacturing procedures, and marketing techniques is carried out to educate the community in order to increase knowledge about the products and its biological processes. Scientifically, in the procedure of utilisation and processing of palm fruit (mayang), there are many science studies found in everyday life. In addition, indirectly, community students as recipients of information can apply it and become home industry producers. This is very helpful for the community in the utilisation of local wisdom in the area considering kolang kaling is a by-product of palm fruit (mayang) processing.
EFFECTIVENESS OF Sargassum sp EXTRACT IN REDUCE BLOOD SUGAR LEVELS AND ACCELERATE WOUND HEALING ON THE SKIN OF DIABETES MELLITUS MICE (Rattus novergicus) Kaihena, Martha; Umagapi, Muhammad Cesar; Kaliky, Arief Rahman Shabri; Tarangi, Filep Marfil; Ukratalo, Abdul Mahid
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p093-100

Abstract

Diabetes Mellitus is a metabolic disorder characterised by elevated blood sugar levels beyond normal ranges, resulting from reduced secretion of the hormone insulin by pancreatic β cells and/or impaired insulin action. Diabetes therapy is often overseen by regulating blood glucose levels regularly and preventing or reducing the risk of complications. Sargassum sp is a variety of brown seaweed native to Indonesia with possible antioxidant and antidiabetic properties. This study seeks to evaluate the efficacy of Sargassum sp extract in lowering blood sugar levels and speeding up wound healing on the skin of diabetic mice. This is an experimental study that uses mice as experimental subjects. The mice were categorised into 5 groups (K-, K+, P1, P2, and P3). Their initial blood sugar levels were recorded, a skin incision was performed, and STZ was administered. When blood sugar levels rise, the K+ group receives metformin; on the other hand, P1, P2, and P3 are administered Sargassum sp extract at a specified dosage. The data were examined using ANOVA and SPSS. The analysis revealed that the water content of Sargassum sp was 4.32%, and the yield value of the concentrated extract was 8.75%. The ethanol extract of Sargassum sp has been revealed to lower blood sugar levels and speed up the wound healing process in mice with diabetes mellitus. The effects observed include decreased blood sugar levels and enhanced wound healing percentage, which are dose-dependent.
Penguatan Pengetahuan Masyarakat Desa Allang Asaude, Kabupaten Seram Bagian Barat tentang Tumbuhan Berkhasiat Obat Tuhumuri, Evelin; Kaihena, Martha; Ukratalo, Abdul M; Mainassy, Meilissa C; Kakisina, Pieter; Pagaya, Joseph; Seumahu, Cecelia Anna; Killay, Amos
Bakti : Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2025): Bakti: Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Layanan Pendidikan Tinggi (LLDIKTI) Wilayah XII Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51135/bakti.5.1.1-9

Abstract

Desa Allang Asaude, yang terletak di Kabupaten Seram Bagian Barat, merupakan daerah yang kaya akan sumber daya alam, terutama keanekaragaman tumbuhan. Masyarakat setempat telah lama memanfaatkan berbagai jenis tumbuhan untuk kebutuhan sehari-hari, khususnya dalam pengobatan tradisional. Kegiatan pengabdian masyarakat ini dilaksanakan dengan tujuan untuk memberikan informasi dan pengetahuan kepada masyarakat Desa Allang Asaude tentang berbagai tumbuhan yang memiliki khasiat sebagai obat. Kegiatan ini dilaksanakan pada hari Sabtu, 11 November 2024. Metode yang digunakan adalah penyuluhan langsung kepada masyarakat melalui ceramah dan diskusi interaktif. Hasil kegiatan menunjukkan bahwa materi yang disampaikan sangat bermanfaat dan berhasil meningkatkan pengetahuan masyarakat mengenai berbagai tumbuhan obat yang ada di sekitar mereka. Masyarakat kini lebih memahami cara pengolahan yang benar dan cara pemanfaatan tumbuhan untuk mengatasi penyakit ringan, seperti demam, batuk, dan gangguan pencernaan. Selain itu, kegiatan ini juga meningkatkan kesadaran masyarakat akan pentingnya melestarikan tumbuhan obat sebagai bagian dari warisan budaya dan sumber daya alam yang perlu dijaga agar dapat dimanfaatkan oleh generasi mendatang.
POTENSI ANTIBAKTERI EKSTRAK ETANOL DAUN KECIPIR (Psophocarpus tetragonolobus (L.) DC) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus IN VITRO Henaulu, Adudin H; Kaihena, Martha
Biofaal Journal Vol 1 No 1 (2020): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.407 KB) | DOI: 10.30598/biofaal.v1i1pp44-54

Abstract

Kecipir (Psophocarpus tetragonolobus (L.) DC) merupakan tanaman polong-polongan (fabaceae) yang sudah lama di kenal masyarakat Indonesia. Buahnya dimanfaatkan oleh masyarakat sebagai sayuran dan pengobatan seperti obat bisul, daunnya secara empiris digunakan sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui potensi dari daun kecipir (P.tetragonolobus(L.) DC)) sebagai antibakteri terhadap pertumbuhan Escherichia coli dan Staphylococcus aureus. Penelitian ini menggunakan daun kecipir segar yang dikeringanginkan dan diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji aktivitas antibakteri terhadap E. coli dan S. aureus menggunakan metode difusi agar dengan pembandingceptazidine 30 μg.Penelitian terdiri dari 6 perlakuan dan 3 kali ulangan yaituP0 (menggunakan aquades steril sebagai control negatif),P1 (konsentrasi EEDK 20%), P2 (konsentrasi EEDK 40%), P3 (konsenterasi EEDK 60%),K4 (konsenterasi EEDK 80%), P5 (konsentrasi EEDK100%) dan P6 (menggunakan ceptazidime 30 μg) sebagai kontrol positif. Hasil penelitian menunjukkan ekstrak etanol daun kecipir berpotensi sebagai antibakteri terhadap E. colidan St. aureus, hal ini dibuktikan dengan adanya zona bening yang dihasilkan EEDK dengan konsentrasi 20;40;60,80 dan 100% untuk E.coli berturut turut dari P0 sampai P6 sebesar0,00; 3.83; 9.86; 12,17;15,63;17;17 dan 17.69. sedangkan besar zona hambat untuk St.aureus berturut turut dari P0 sampai P6 sebesar 0,00; 0,00; 3,67; 4,55; 5,33; 6.17 dan 13.83dimana EEDK lebih berpotensi menghambat E. coli dibandingkan St. aureus
Uterine Performance of Rats Exposed to Cigarette Post-Therapy Ethanol Extract of Kebar Grass (Biophytum petersianum Klotzsch) Unitly, Adrien Jems Akiles; Kaihena, Martha; Kubela, Dormatina
Devotion : Journal of Research and Community Service Vol. 4 No. 6 (2023): Devotion: Journal of Research and Community Service
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/devotion.v4i6.492

Abstract

This study aims to determine the effect of giving ethanol extract of kebar grass (Biophytum petersianum Klotzsch) on the uterine performance of Rattus norvegicus rats exposed to cigarette smoke. The experimental design used was a completely randomized design (CRD). The results obtained were analyzed by Analysis of Variance (ANOVA) then continued with Duncan's test at a significant level of α = 0.05 using SAS software. The sample consisted of 12 rats, which were divided into 4 groups. The rat group (+), 0.067, 0.0135, decreased uterine function by exposure to 10 cigarettes/head/day for 28 days. Mice (-) were the negative control group, namely rats that were not exposed to cigarette smoke and ethanol extract of coriander grass. Mice (+) are the positive control group, namely rats exposed to cigarette smoke 10 cigarettes/head/day for 28 days. Groups 0.067 and 0.135 were exposed to cigarette smoke 10 cigarettes/head/day for 28 days and then given 0.067 mg of coriander grass ethanol extract for the 0.067 group and a dose of 0.135 mg for the rat group 0.135. The results showed that there was an effect of increasing the performance of the rat uterus after administration of ethanol extract of coriander grass. It can be concluded that administration of the ethanol extract of the grass Biophytum petersianum Klotzsch kebar can increase uterine weight and uterine length of rats, with a good dose of 0.135 mg.
Electric Mosquito Repellent Effect of Male Breadfruit Flower Mat Causes Mosquito Mortality Anopheles sp. Moniharapon, Debby Dijola; Kaihena, Martha; Unitly, Adrien Jems Akiles
Devotion : Journal of Research and Community Service Vol. 4 No. 6 (2023): Devotion: Journal of Research and Community Service
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/devotion.v4i6.494

Abstract

Through mosquito bites, parasites of the genus Plasmodium spread the disease known as malaria. Malaria-carrying mosquitoes of the genus Anopheles sp. One secure method of controlling the Anopheles sp. mosquito population is biological mosquito control. Since the biological method does not use chemicals but rather the phytochemicals found in plants, one of which contains compounds from the male breadfruit flower and is capable of acting as a vegetable larvicide or mosquito repellent. The aims of the study is to evaluate the effectiveness of electric mosquito mats that contain male breadfruit flowers to kill or cause mosquitoes (Anopheles sp.) to die. A completely randomized design (CRD) was used for the experiment. The results showed a change in the mortality of Anopheles sp. mosquito larvae. After exposure to breadfruit flower mat doses of 0.5gr, 1gr, 1.5gr, 2gr compared to no administration. Thus, it was concluded that male breadfruit flower mats with concentrations of 0.5gr, 1gr, 1.5gr, and 2gr had an insecticidal effect on the mortality of Anopheles sp. mosquitoes. with an effective concentration of 2gr (96.5% mortality), of which 50% mortality (LC50) of Anopheles sp. larvae. Occurred at a concentration of ethanol extract of male breadfruit flowers dose 0.36gr.
EFFECTIVENESS OF FINGER LEAF INFUSION (Psophocarpus tetragonolobus (L.) DC) ON THE MALARIA MODEL OF RATS (Rattus norvegicus) Kaihena, Martha; Samson, Efraim
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p025-032

Abstract

Winged bean is a type of plant that is used as traditional medicine. This study aims to determine the effectiveness of winged bean leaf infusion (Psophocarpus tetragonolobus(L.) DC) against white rat (Rattus norvegicus) model of malaria. The method used in this research is completely randomized design with 4 treatment groups and 3 replications consisting of a control group without being given an infusion (P1), a group that was given an infusion of concentrations: 31.25 mg/mL (P2), 62.5 mg/ mL(P3), and 125 mg/mL (P4). This study used 20 male rats, of which 5 rats including as donor mice. Donor mice were infected with Plasmodium berghe and left until the parasitemia percentage reached> 20%. Then 4 groups of model mice were infected with Plasmodium berghei. Observations were made for 7 days, starting from day 0 (before treatment), 4 days during treatment and 2 days after treatment. The percentage of parasitemia was calculated starting from the day before administration of winged bean leaf infusion (IDK) until the 7th day. The results of this study indicate that IDK can inhibit parasite growth by reducing the level of parasitemia along with increasing concentrations, namely (P2) of 73.78%; (P3) of 89.33%; and (P4) of 93.69%. It can be concluded has potential in inhibiting the growth of Plasmodium berghei in the rat model of malaria with an effective concentration of 31.25 mg/mL.
Keamanan Dan Keselamatan Kerja (K3) Di Laboratorium IPA Pada SMP Negeri 7 SATAP Lapu, Petrus; Riupassa, Pieter A.; Pesik, Anneke; Kaihena, Martha; Patty, Kristi Lenci; Hiariej, Adriana
PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya Vol 1 No 2 (2023): PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya
Publisher : Jurusan Matematika FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pengamatanv1i2p35-42

Abstract

Keamanan dan keselamatan kerja (K3) Laboratorium adalah semua upaya untuk menjamin keselamatan dan kesehatan pekerja laboratorium dari risiko-risiko yang ada di laboratorium. Kecelakaan di laboratorium dapat terjadi akibat kurangnya pengawasan, pengguna laboratorium kurang memahami bahaya dan cara menanganinya ketika terjadi kecelakaan yang dapat berdampak fatal bagi praktikan dan lingkungan sekitarnya. Kegiatan pengabdian kepada masyarakat kepada guru dan siswa SMP Negeri 7 SATAP Leihitu berupa sosialisasi pentingnya keaemanan dan keselamatan kerja (K3) di laboratorium dengan tujuan untuk meningkatkan pemahaman tentang pentingnya K3 di laboratorium sehingga dapat meminimalkan kecelakaan yang terjadi di laboratorium.
Pembuatan Preparat Tumbuhan Sebagai Media Pembelajaran IPA Biologi Bagi Siswa SMP Negeri 7 SATAP Leihitu Hiariej, Adriana; Kaihena, Martha; Riupassa, Pieter Agusthinus; Lapu, Petrus; Pesik, Anneke
PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya Vol 3 No 1 (2025): PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya
Publisher : Jurusan Matematika FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pengamatanv3i1p01-08

Abstract

Plant cell differentiation can only be understood visually by students through observation with a microscope. The ability of junior high school students to use microscopes is still limited so that practicum activities for making preparations are needed. This activity aims to improve student understanding and skills about monocular and binocular microscopes and making plant preparations to observe stomatal and epidermal cells. The implementation method includes lectures, interactive discussions, question and answer sessions, and making fresh plant preparations and proper microscope usage techniques. The results of the activity showed that participants were able to operate a microscope, make fresh plant preparations, and observe cells. The level of enthusiasm of the participants was quite high, especially when they managed to observe stomatal and epidermal cells directly through the microscope.