Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Kultivasi Caraka Tani: Journal of Sustainable Agriculture
Ezura, Hiroshi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
The effect of fruit maturation stage on seed germination of iaa9-3 and iaa9-5 tomato mutants Al Aufa, Elfa Muhammad Ihsan; Mubarok, Syariful; Nuraini, Anne; Budiarto, Rahmat; Waluyo, Nurmalita; Ezura, Hiroshi
Kultivasi Vol 23, No 1 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i1.40859

Abstract

One of the internal factors that play an important role in seed quality is the level of the phytohormone auxin. Efforts to increase auxin in seeds include developing new varieties with increased indigenous auxin, such as those found in mutant tomatoes from Micro-Tom, namely iaa9-3 and iaa9-5. This research was conducted to determine the germination response of iaa9-3 and iaa9-5 mutant tomato seeds at different levels of the fruit maturation stage. The research was carried out at the Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University, and the Seed Testing Laboratory of the Center for Standard Testing of Vegetable Plant Instruments. The response design used was a completely randomized factorial design consisting of two factors and repeated three times. The first factor was the iaa9-3, iaa9-5, and Wild-Type Micro-Tom (WT-MT) mutants as controls. The second factor is the fruit maturation stage, which consists of Breaker, Pink, and Red. The research results showed that there was an interaction between genotype and fruit maturation stage on the parameters of germination, growth simultaneity, and hypocotyl length.  The harvest stage for tomatoes to produce normal strong seed germination was the pink stage in all tomato genotypes tested. The best germination rate was shown by WTMT seeds at the pink stage, genotype iaa9-3 at the red stage, and genotype iaa9-5 at the red or pink stage. The effect of fruit maturity stage on the synchronization of sprout growth was relatively not significantly different in mutant tomatoes, but it had an effect in WTMT tomatoes, namely the best pink stage.
Tomato Mutants SlIAA9 Exhibit Thermo-Morphophysiological Characters and Enhanced SIDREBA4 Gene Expression Rafsyanyani, Cory; Anjalani, Titah Rigel; Rahayu, Anisa Esti; Mubarok, Syariful; Widiastuti, Ani; Matra, Deden Derajat; Ezura, Hiroshi; Jadid, Nurul
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 1 (2026): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i1.108173

Abstract

Rising temperatures associated with climate change threaten tomato productivity, yet the contribution of auxin signaling components to heat-stress adaptation remains incompletely understood. The IAA9 gene, encoding an Aux/IAA transcriptional repressor, is well known for its role in auxin-regulated development, but its role in heat responses is still unclear. This study aims to elucidate the function of IAA9 in modulating tomato responses under heat stress conditions. Researchers utilized tomato iaa9-3 and iaa9-5 mutants and exposed them to prolonged elevated temperatures of 40 to 45 °C for 6 weeks to assess morphophysiological traits, and to 38 to 40 °C for 6 days to evaluate molecular responses through SlDREBA4 gene expression analysis. Under prolonged heat stress, all genotypes exhibited reduced leaf area, leaf number, and total chlorophyll content, accompanied by increased plant height compared to plants grown under normal conditions. Specifically, wild-type Micro-Tom (WT-MT) showed the lowest values in leaf area (165.89 cm²), leaf number (23 leaves), and total chlorophyll content (115.7 µg g-1). In contrast, the iaa9-3 and iaa9-5 mutants recorded the highest plant heights at 11.98 and 12.13 cm, respectively, indicating a differential growth response under stress. Gene expression analysis revealed that SlDREBA4 expression was upregulated in both iaa9-3 and iaa9-5 mutants compared to normal temperature conditions, with increases of 0.45-fold and 1.78-fold, respectively. These results indicate that IAA9 mutations confer enhanced thermotolerance in tomato, as reflected by altered morphology and increased heat-responsive gene expression. This study highlights IAA9 as a potential genetic target for improving heat stress resilience in tomato breeding programs.
Co-Authors Al Aufa, Elfa Muhammad Ihsan Ani Widiastuti, Ani Anjalani, Titah Rigel Anne Nuraini Matra, Deden Derajat Nurul Jadid Rafsyanyani, Cory Rahayu, Anisa Esti Rahmat Budiarto Syariful Mubarok Waluyo, Nurmalita