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All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Journal of Tropical Crop Science
Matra, Deden Derajat
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Interaksi Nisbah Daun: Buah dan Pupuk Daun pada Kualitas Buah Abiu (Pouteria caimito Radlk.) Thamara, Aria; Susanto, Slamet; Matra, Deden Derajat; Widodo, Winarso Drajad
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 4 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.4.519

Abstract

Abiu (Pouteria caimito Radlk.) is a plant of Sapotaceae family that can be consumed fresh, processed, or as a medical product. The fruit and leaves of this plant contain bioactive compounds. This research aims to inform the interaction between leaf:fruit ratio treatment and foliar fertilizer on the quality of the abiu fruit. The study was conducted at Kebun Balungbang Jaya, Dramaga, Bogor, West Java Province, from July 2022 to November 2022. The experiment used a factorial split plot design. The main plot was a leaf:fruit ratio treatment (N1, 45 leaves:1 fruit, and N2, 30 leaves:1 fruit) on each tertiary branch, and the tiller plot was foliar fertilizer (P1: 1 g L-1, P2: 2 g L-1, and P3: 3 g L-1), which consisted of 3 replications. The results showed that treatment of the leaf-fruit ratio and foliar fertilizer did not affect the quality of the abiu fruit. The interaction between leaf:fruit ratio and foliar fertilizer had a significant effect on ATT, as indicated by the lowest acidity value in the leaf:fruit ratio treatment of 45:1 and a fertilizer concentration of 1 g L-1 with a value of 0.12% but has no significant effect on the external and internal quality of other fruits. Keywords: Bioactive compounds, sapotaceae, tertiary branch, titratable acidity
Pengaruh Kombinasi Media dan Lebar Keratan terhadap Pertumbuhan Cangkok Jeruk Pamelo (Citrus maxima (Burm.) Merr.) Widyaswara, Muhammad Heraldi Garda; Susanto, Slamet; Matra, Deden Derajat
Buletin Agrohorti Vol. 12 No. 3 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i3.51581

Abstract

Pamelo (Citrus maxima (Burm.) Merr.) merupakan buah yang banyak dibudidayakan di kawasan Asia, termasuk Indonesia. Perbanyakan pamelo lebih efektif dilakukan secara vegetatif karena memiliki keunggulan pada sifat anakan yang serupa dengan induknya. Cangkok merupakan salah satu cara perbanyakan vegetatif yang banyak digunakan karena memiliki keberhasilan berakar yang tinggi pada tanaman berkayu. Penelitian bertujuan mengevaluasi efek media dan lebar keratan terhadap keberhasilan cangkok pamelo. Penelitian dilakukan di Kebun Percobaan Cikabayan, Institut Pertanian Bogor, pada bulan April hingga November 2021. Percobaan dilakukan menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal terdiri atas sembilan perlakuan kombinasi. Perlakuan yang digunakan adalah jenis media (pupuk kandang dan tanah (1:1), arang sekam dam serbuk sabut kelapa (1:1), dan sphagnum moss dan tanah (1:1)), masing-masing dikombinasikan dengan lebar keratan (3 cm, 6 cm, dan 9 cm), menghasilkan 9 kombinasi perlakuan. Hasil percobaan menunjukkan kombinasi perlakuan media dan lebar keratan berpengaruh nyata terhadap keberhasilan cangkok. Kombinasi perlakuan media arang sekam dan cocopeat (1) dan lebar keratan 3 cm merupakan kombinasi perlakuan dengan persentase keberhasilan cangkok paling tinggi dan memiliki rata-rata jumlah akar paling banyak dibandingkan kombinasi perlakuan lainnya. Kata kunci: cocopeat, sphagnum moss, perbanyakan vegetatif, jumlah akar, panjang akar
Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) Magandhi, Mahat; Sobir; Matra, Deden Derajat; Wahyu, Yudiwanti; Sudarmono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.132-139

Abstract

Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources.
Development of Few Significant SNP Markers from Transcriptomic Data for Selection of Sengon (Falcataria falcata (L.) Greuter and R. Rankin) Resistant to Boktor Stem Borer and Gall Rust Disease Nugroho, Aditya; Anita, Vilda Puji Dini; Matra, Deden Derajat; Siregar, Iskandar Zulkarnaen; Siregar, Ulfah Juniarti
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.110-125

Abstract

Sengon (Falcataria falcata (L.) Greuter and R. Rankin) plantations in Indonesia are threatened by attacks from Boktor stem borers and gall rust disease. Controlling pests and diseases is difficult therefore, planting resistant trees obtained from tree selection programs is necessary. Currently, genomic breeding often incorporates GWAS, which uses thousands of SNP markers to identify markers with significant associations with the traits studied. This study aimed to bypass such expensive studies by identifying and developing SNP markers from sequences of putative resistance genes to Boktor stem borer and gall rust disease, identified from sengon transcriptomic data analysis. A total of 496,194 putative SNP sites were identified from transcriptomic sequences using the SAMtools and BFCtools programs, of which 119 SNP sites were associated with resistance genes. Of the 101 non-synonymous SNPs selected, only 12 were located in the conserved domain of each gene and were used for primer design. Of the 13 primers designed, only 10 were successfully amplified. Validation of 10 developed SNP markers on 100 sengon accessions using the HRM method confirmed a significant association between SNP markers and resistance traits, with a -log 10 (P-value) between 10.49 and 16.63. A few SNPs markers developed from putative resistance gene sequences are associated with resistance traits in sengon. Therefore, the SNP markers could be applied in selection programs for sengon trees resistant to Boktor stem borers and gall rust disease.
Growth Response, Physiology, Metabolomic, and Production of Micro-Tom Tomatoes to Additional Lighting with White and Purple Light-Emitting Diode Lubis, Wahyu Muhammad Yuha; Hapsari, Dhika Prita; Poerwanto, Roedhy; Matra, Deden Derajat
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.284-295

Abstract

Tomatoes are a widely produced and consumed fruit-vegetable belonging to the Solanaceae family. It contains minerals, vitamins, essential amino acids, sugars, and fiber. Micro-Tom is a wild-type cultivar from a cross between the Florida Basket and Ohio 4013-3 cultivars. Micro-Tom has two mutant types, the iaa9-3 and iaa9-5 mutants, which exhibit strong parthenocarpic properties. It is classified as a mutant tomato due to a mutation in the IAA9 gene, which belongs to the Auxin/IAA (Indole-3-Acetic-Acid) gene family and plays a role in suppressing the endogenous auxin signal transcription pathway. Using artificial light in cultivation techniques shortens the plant cycle and accelerates the juvenile phase. This study aimed to investigate the morphological, physiological, and production responses of Micro-Tom tomatoes to supplemental LED lighting. The research was conducted at the Leuwikopo experimental field of IPB University from March to November 2023. A completely randomized design was employed, with two factors (LED spectrum and Micro-Tom genotyping) and nine replicates. The study also examined the potential benefits of combining polychromatic and monochromatic light-emitting diodes (LEDs). The response of Micro-Tom tomatoes to artificial lighting with purple and white LEDs did not show significant differences in growth and production parameters. However, significant differences were observed between the tomato types, specifically between the Wild-type and the iaa9-3 mutant. No significant differences were found in LED treatment or Micro-Tom genotype for physiological parameters such as chlorophyll content and glucose-fructose levels. Regarding fruit quality, no significant differences were observed for parameters such as total soluble solids (TSS, Brix) to total titratable acidity (TTA, acidity), glucose and fructose, and malic acid. The LC-MS/MS analysis of leaves exposed to purple LED light revealed a profile dominated by secondary metabolites from the fatty acid compound group, suggesting the potential benefits of this lighting combination. In conclusion, using LED lights can accelerate the plant life cycle and shorten the juvenile phase, as evidenced by the first flower emergence, which occurred 20 days after transplanting (DAT) in the wild-type genotype and 16 DAT in the mutant genotype.
Keragaman Genetik Matoa (Pometia pinnata) menggunakan Penanda Molekuler SSR Saputra, Maliki Febriantono; Hapsari, Dhika Prita; Matra, Deden Derajat
Buletin Agrohorti Vol. 13 No. 1 (2025): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v13i1.61774

Abstract

Indonesia adalah negara yang kaya akan keanekaragaman hayati dengan berbagai jenis spesies tumbuh-tumbuhan. Keanekaragaman tersebut terdiri dari tanaman buah yang salah satunya berasal dari buah Matoa (Pometia pinnata). Matoa merupakan tanaman dari famili Sapindaceae yang tersebar di wilayah tropis. Tanaman matoa banyak dimanfaatkan sebagai obat-obatan tradisional yang diketahui mengandung kelompok senyawa diantaranya flavonoid, tanin, dan saponin. Analisis keragaman genetik matoa menjadi informasi dasar untuk pelaksanaan kegiatan seleksi dan persilangan lanjutan dalam program pemuliaan matoa. Marka mikrosatelit merupakan salah satu metode analisis marka molekuler kodominan. Tujuan dari penelitian ini untuk melakukan seleksi primer ssr untuk analisis kekerabatan 10 aksesi matoa Dramaga dengan 4 outgrup Pometia pinnata kalimantan melalui pendekatan marka mikrosatelit. DNA diisolasi menggunakan CTAB. Sepuluh primer mikrosatelit yang digunakan adalah Pp.e.6402R(L), Pp.e.66857R(L), Pp.e.80271R(L), Pp.e.89451R(L), Pp.e.115726R(L), Pp.e.161167R(L), Pp.e.186462R(L), Pp.e.187105R(L), Pp.e.238124R(L), Pp.e.287861R(L). Amplifikasi mengacu pada protokol ThermoScientific DreamTaq Green PCR Master Mix. Rata-rata alel yang didapat dari 10 lokus yang diuji ± 2. Ditemukan 1 pasang lokus yang memiliki NA paling rendah yaitu lokus Pp.e.287861R(L) yang memiliki alel 1, dan hanya 2 DNA yang dapat diamplifikasi pada 10 pasang lokus primer yang digunakan, yaitu DNA yang berasal dari aksesi IPBA8, IPBA9. Kata kunci: alel, genetik, keanekaragaman genetik, marka mikrosatelit, tanaman buah
Co-Authors Aditya Nugroho Anita, Vilda Puji Dini Dhika Prita Hapsari ISKANDAR ZULKARNAEN SIREGAR Lubis, Wahyu Muhammad Yuha Mahat Magandhi Roedhy Poerwanto Saputra, Maliki Febriantono Slamet Susanto Sobir Sobir Sudarmono Thamara, Aria Ulfah Juniarti Siregar Widyaswara, Muhammad Heraldi Garda Winarso D. Widodo Yudiwanti Wahyu E. Kusumo