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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Teknologi Pangan dan Gizi Jurnal Sumberdaya Hayati Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Filailla, Euis
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Immunology & microbiology

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PENGARUH PENAMBAHAN STARTER DAN WAKTU INKUBASI: DARK COKLAT (Theobromo cacao L.) TERHADAP AKTIVITAS ANTIOKSIDAN DAN KUALITAS MINUMAN PROBIOTIK Mulyani, Hani; Sundowo, Andini; Filailla, Euis; Ernawati, Teni
Jurnal Teknologi Pangan dan Gizi Vol 18, No 1 (2019)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.33508/jtpg.v18i1.1984

Abstract

Pada pembuatan yogurt terjadi konversi gula susu (laktosa) menjadi asam laktat melalui proses fermentasi oleh bakteri asam laktat (BAL). Konsentrasi stater dan waktu fermentasi yang digunakan akan mempengaruhi kecepatan perombakan laktosa menjadi asam laktat. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan stater dengan variasi sebesar : 1, 5, 10, 15 dan 20% dan waktu fermentasi pada 24 jam dan 48 jam terhadap asam laktat. Parameter yang diuji diantaranya adalah pH, kadar laktosa, kadar protein, kadar asam lakat, dan aktivitas antioksidan. Parameter yang diuji dari hasil proses fermentasi adalah pH, laktosa, asam laktat, protein terlarut. Pengujian laktosa dan asam laktat dilakukan dengan menggunakan HPLC, pengujian konsentrasi protein terlarut dengan menggunakan metode Lowry. Asam laktosa dan asam laktat tertinggi diperoleh dari fermentasi dengan variasi konsentrasi BAL starter 15% dalam waktu fermentasi selama 24 jam. Hasil analisis % inhibisi pada yogurt konsentrasi stater 10% dan waktu fermentasi 48 jam tanpa penambahan dark coklat dan penambahan dark coklat menunjukkan % inhibisi antioksidan paling tinggi dibandingkan dengan yang lainnya sebesar 20,84 % dan 95,58%. Hasil penelitian Variasi starter konsentrasi BAL dan lamanya proses fermentasi tidak mempengaruhi pH dan kadar protein terlarut.
Antidiabetic Activity of Cocor Bebek Leaves (Kalanchoe pinnata Lam.Pers.) Ethanolic Extract from Various Areas Dewiyanti, Indah Dwiatmi; Filailla, Euis; Megawati, Megawati; Yuliani, Tri
Journal of Tropical Life Science Vol 2, No 2 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antidiabetic activity of Cocor Bebek leaves (Kalanchoe pinnata Lam.Pers.) ethanolic extract from Bogor city, Kabupaten Bogor and south of Tangerang city has been studied. The study was conducted in vitro using glucosidase inhibitor method. The results of the study showed that IC50 of the extract from Bogor city, Kabupaten Bogor, and Tangerang Selatan city is 40.94 ppm, 33.58 ppm and 16.12 ppm respectively. Meanwhile, IC50 of quersetin which has antidiabetic activity is 10.22 ppm. The results showed that Cocor Bebek leaves (Kalanchoe pinnata Lam.Pers.) ethanolic extract had antidiabetic activity with IC50 less than 100 ppm. However, the activity is lesser than quercetin.Keywords:Kalanchoe pinnata Lam.Pers, antidiabetic activity, glucosidase inhibitor
Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.) : Antibacterial and Antioxidant Properties of Bioactive Compounds Derived from Endophytic Bacteria Isolated from Nyatoh (Palaquium amboinense B.) Putra, Rayhan Helmyana; Retnowati, Dwi; Cordova, Datu Muhammad; Zahra, Aliya Azkia; Primahana, Gian; Dewi, Rizna Triana; Filailla, Euis; Sukirno; Prastya, Muhammad Eka
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.25-32

Abstract

Nyatoh plant (Palaquium amboinense B.) is native to Indonesia which is known to have the ability to produce potential secondary metabolites. The aim of this study was to evaluate the antibacterial and antioxidant activities from bioactive compounds produced by endophytic bacteria from nyatoh leaves. Five endophytic bacteria were tested against 4 targeted bacteria including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659, and Staphylococcus aureus ATCC 6538. Bacterial isolate which had the strongest antibacterial activity was then fermented and extracted. The corresponding extract was further tested on disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, antioxidant evaluation, and analyzed for its bioactive compound components via GC-MS analysis. The results showed that one isolate, namely D1 can produce secondary metabolite which act as antibacterial and antioxidant potentials. This corresponding extract has the strongest MIC and MBC against B. subtilis with value of 39.5 and >78.1 µg/ml and exhibited antioxidant activity against 2,2-difenil-1-pikrilhidrazil (DPPH) with inhibition value of 85.37%. Based on GC-MS analysis, secondary metabolites derived from this potential extract contained some major active constituents including Tributyl acetylcitrate and bis (2-ethylhexyl) ester which might act as antibacterial and antioxidant agents.
Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract Budiari, Setyani; Hamidatun, Hamidatun; Filailla, Euis; Artanti, Nina; Devi, Anastasia Fitria; Melanie, Hakiki; Maryati, Yati; Mulyani, Hani; Susilowati, Agustine; Aspiyanto, Aspiyanto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 3 (2023): December 2023
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.788

Abstract

Chlorella sp. microalgae is a potential source of natural antioxidants and other bioactive compounds used in the food and pharmaceutical industries. Ultrasound pretreatments followed by enzymolysis are widely used to improve the functional properties of biological materials. This work aimed to investigate the effects of ultrasound pretreatments followed by bromelain hydrolysis on the proteins, phenolic content, and antioxidant activity of Chlorella sp. crude extract. The experimental design for the study involved two factors: ultrasound pretreatment time (0, 5, and 10 min) and bromelain hydrolysis time (0, 2, 4, and 6 hours). The protein was measured using Lowry assay while the total phenolic content was measured using a Folin–Ciocalteu assay. The highest protein content (7.09 ± 0.44 mg/mL) and phenolic concentrations (0.28 ± 0.01 ìg/mL) were obtained at 5 min ultrasound pretreatment and 6 hours enzymolysis. For antioxidant activity, 5 min ultrasound pretreatment followed by 4 hours of enzymolysis resulted in the highest DPPH (1,1-diphenyl-2-picrylhydrazyl radical) inhibitory activity (81.74%). Meanwhile, ultrasound pretreatment for 10 min followed by 6 hours of enzymolysis resulted in the highest ABTS (2,20 azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) inhibitory activity (94.10%). This study showed that variation in ultrasound pretreatments followed by bromelain hydrolysis could be used to improve the protein content, phenolic content, and antioxidant activity of Chlorella sp. crude extract. 
Co-Authors Agustine Susilowati Andini Sundowo Aspiyanto Aspiyanto Budiari, Setyani Cordova, Datu Muhammad Devi, Anastasia Fitria Dewiyanti, Indah Dwiatmi Dwi Retnowati Hakiki Melanie Hamidatun Hamidatun, Hamidatun Hani Mulyani, Hani Megawati - Nina Artanti Prastya, Muhammad Eka Primahana, Gian Putra, Rayhan Helmyana Rizna Triana Dewi SUKIRNO Teni Ernawati Tri Yuliani, Tri Yati Maryati Zahra, Aliya Azkia