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All Journal Jurnal Natur Indonesia Jurnal Perlindungan Tanaman Indonesia Indonesian Journal of Agricultural Research Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Journal of Saintech Transfer
Saputra, Rachmad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Environmental Science Social Sciences Veterinary Other

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Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro Elfina, Yetti; Ali, Muhammad; Saputra, Rachmad
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.615 KB) | DOI: 10.31258/jnat.16.2.79-90

Abstract

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.
PEMBERDAYAAN KELOMPOK TANI DALAM PENGENDALIAN PENYAKIT TANAMAN DENGAN TEKNOLOGI BIOFUNGISIDA PLUS DI KABUPATEN KAMPAR Puspita, Fifi; Saputra, Rachmad; Adiwirman, Adiwirman; Ali, Muhammad; Idwar, Idwar; Armaini, Armaini; Nurbaiti, Nurbaiti; Yoseva, Sri; Zuhry, Elza
Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Vol 7 No 2 (2020): Mei
Publisher : Lembaga Penelitian, Penerbitan dan Pengabdian Masyarakat (LP3M) UNSIQ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/ppkm.v7i2.963

Abstract

Kabupaten Kampar merupakan satu diantara beberapa sentra penanaman padi di Provinsi Riau. Kendala dalam melaksanakan budidaya tanaman padi yang masih ditemukan diantaranya adalah kurangnya pengetahuan petani tentang teknik budidaya dan pengendalian penyakit pada tanaman padi. Tujuan kegiatan ini ialah untuk mendorong petani agar dapat membuat biofungisida berbahan aktif konsorsium Trichoderma virens dan Pseudomonad berflourescens dalam formulasi Biofungisida Plus untuk pencegahan dan pengendalian penyakit yang menyerang tanaman padi. Kegiatan pemberdayaan petani di Desa Sawah melalui penerapan teknologi pengendalian penyakit tanaman padi dengan biofungisida plus dilaksanakan dengan menerapkan Model Community Development. Pada metode ini secara langsung dilibatkan sebagai subyek dan obyek dalam pelaksanaan kegiatan. Hasil yang diperoleh dari kegiatan pemberdayaan masyarakat ini diantaranya ialah, masyarakat bersedia untuk menerapkan teknologi Biofungisida Plus di lahan sawahnya. Berdasarkan hasil kuisioner juga diketahui pemahaman dan wawasan petani bertambah setelah diperkenalkan dengan Teknologi Biofungisida Plus ini. Aplikasi Biofungisida Plus di lahan petani menunjukkan kondisi tanaman yang lebih baik dibandingkan dengan teknik budidaya yang biasa digunakan oleh petani.
Assistance for Oyster Mushroom Entrepreneurs in Making Fish Feed Formulations for African Night Crawler Worm (ANC) and Trichoderma sp. Fermented Bran Dini, Isna Rahma; Saputra, Rachmad; Hapsoh, Hapsoh; Salbiah, Desita; Yoseva, Sri; Masjudi, Heri
Journal of Saintech Transfer Vol. 6 No. 2 (2023): Journal of Saintech Transfer
Publisher : Talenta Publisher Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jst.v6i2.10223

Abstract

Utilization of baglog waste as a growth medium for ANC worms as a raw material for making formulated fish feed provides opportunities as a side business for oyster mushroom entrepreneurs to increase income. The purpose of this service is to provide assistance to target partners, in this case oyster mushroom entrepreneurs, in utilizing ANC worm flour as a substitute for fish meal and then formulated with Trichoderma sp. fermented bran. The results of the dedication that has been carried out show that there has been an increase in partners' skills in making formulated fish fefazidah@usu.ac.ided and developing the potential of science and technology-based independent entrepreneurs who develop from mushroom businesses. After this dedication, it is hoped that the partners can continue to develop this worm farming business and the application of worm-formulated feed for fish farming which has also been developed by partners so as to create an integrated oyster mushroom-based farming center.
Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro Saputra, Rachmad; Arwiyanto, Triwidodo; Wibowo, Arif
Indonesian Journal of Agricultural Research Vol. 2 No. 3 (2019): InJAR, Vol. 2, No. 3, November 2019
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1041.563 KB) | DOI: 10.32734/injar.v2i3.1389

Abstract

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.
Molecular Characterization of Mungbean yellow mosaic India virus and Tomato leaf curl New Delhi virus in Sukoharjo and Magelang Regencies, Indonesia Surwadinata, Antama; Putro, Ananda Restu; Pradhana, Adhi; Shalihah, Filzah; Pancasona, Muhammad Garda; Saputra, Rachmad; Haj Ali, Mayadah A.; Santosa, Adyatma Irawan
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.101371

Abstract

Diseases caused by Begomoviruses in black-eyed pea (Vigna unguiculata subsp. unguiculata) and cucumber (Cucumis sativus) are remains insufficiently explored in Indonesia. One symptomatic black-eyed pea and two cucumber samples were collected from Sukoharjo and Magelang Regencies, respectively, to be molecularly tested using Krusty/Homer and SPG1/SPG2 primer pairs for universal detection of Begomoviruses. NCBI BLAST analysis on the obtained nucleotide sequences confirmed mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) infection in black-eyed pea sample and tomato leaf curl New Delhi virus (ToLCNDV, Begomovirus solanumdelhiense) infection in both cucumber samples. Sequences of partial AV1, and partial AC1 and AC2 genes of the three isolates were registered with accession nos. PQ539469-71 and PQ539476-78, respectively, in NCBI GenBank. No recombination signal was detected in the sequences of the new isolates according to scan by Recombination Detection Program (RDP v.5.30). In the phylogenetic trees built by MEGA 11 software with Tamura-Nei parameter model, MYMIV H-2 as well as ToLCNDV N-8 and V-97 isolates shared basal nodes with Indonesian isolates, indicated their close genetic relationship with other isolates also found in the country. While expanding our information regarding genetic diversity of Begomoviruses, this study also reported the first cases of MYMIV in black-eyed pea in Indonesia and ToLCNDV in cucumber in Magelang, to the best of our knowledge.
Co-Authors Adiwirman Adiwirman, Adiwirman Adyatma Irawan Santosa Arif Wibowo Armaini, Armaini Desita Salbiah Elza Zuhry Fifi Puspita Haj Ali, Mayadah A. Hapsoh Idwar, Idwar Isna Rahma Dini Masjudi, Heri MUHAMMAD ALI Nurwijayanti Pancasona, Muhammad Garda Pradhana, Adhi Putro, Ananda Restu Shalihah, Filzah Sri Yoseva Surwadinata, Antama Triwidodo Arwiyanto Yetti Elfina