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Kemampuan Kitinase Streptomyces RKt5 sebagai Antijamur terhadap Patogen Fusarium oxysporum Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The purpose of the reasearch is to determine of antifungal activity from chitinase from Streptomyces RKt5 to inhibite growth of Fusariumoxysporum. The chitinase of Streptomyces RKt5 produced in liquid chitin medium with optimum conditions (inoculum concentration, pHand incubation time) and then partially purified with ammonium sulphate. The enzyme products were tested the antifungal activity againstF.oxysporum. The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test. Thepartial purified chitinase enzyme couldn’t inhibit the fungal growth. But if the mycellium fragmented, the enzyme could degrade the fungalcell wall in incubation time. The frequency of fungal cell wall lysis and levels of N-acetylglucosamine released that have been increasingalong with the length of incubation time.

Eksplorasi dan Bioasai Berbagai Isolat Bacillus thuringiensis Lokal terhadap Larva Beberapa Jenis Serangga Suryanto, Dwi; Chairani, Chairani; Rusika, Deddy; Lubis, Nita A.; Yurnaliza, Yurnaliza
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.

Kondisi Optimum untuk Produksi Kitinase dari Streptomyces Rkt5 dan Karakterisasi pH dan Suhu Enzim Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.

The utilization of organic waste into fermented goat fodder in Tanjung Rejo village, Deli Serdang regency Yeanny, Mayang Sari; Yurnaliza; Hannum, Saleha
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2019): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Community service activities with the title of The Utilization of Organic Waste into Fermented Goat Fodder in Tanjung Rejo Village, Deli Serdang Regency will be held in May - November 2019.The purpose of this community service is to utilize organic waste into fermented goat fodder in Tanjung Rejo village.Organic waste such as straw, banana tree and others can be converted into fermented goat fodder which is economical, nutritious and environmentally friendly.The specific target of this community service is that the fermented goat fodder produced can be stored for a long time with good quality.The method used is a combination of counselling, training, hands-on practice and work evaluation.The procedures of making fermented goat fodder are as follows; (1) Chop straw or banana tree using a chopper machine, this stage serves to make the fermentation process easier. (2) Prepare the place of manufacture, can use tarpaulins, large plastic or large barrels (3). Dissolve sugar or sugar cane with water plus SOC-HCS probiotic solution. (4) Enter the straw, husk and bran at the place of preparation that has been prepared previously. (5) Flush all materials that have been arranged evenly in the place of manufacture with a solution of sugar and SOC-HCS that had been dissolved with water. (6) Mix all ingredients evenly. (7) After everything has been stirred evenly, then cover the place of manufacture with a tarp or with anything, make sure to be airtight. (8) Wait for 1 - 14 days. The fermented fodder that you have made from straw will be ready or if the fermentation feed is made using a banana tree, it can be faster.All products produced can be used for their own needs or sold according to the needs of the people of Tanjung Rejo village, especially the Tanjung Rejo goat breeders group.

EFEKTIVITAS BERKUMUR LARUTAN EKSTRAK ETANOL KULIT KAYU MANIS DALAM MENURUNKAN AKUMULASI PLAK GIGI Syahdiana Waty; Dwi Suryanto; Yurnaliza Yurnaliza
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 12 No. 1 (2017): Jurnal Ilmiah PANNMED Periode Mei - Agustus 2017
Publisher : Poltekkes Kemenkes Medan

Kulit kayu manis merupakan salah satu obat tradisional yang diduga mengandung beberapa senyawa antibakteri seperti flavonoid, saponin dan cinnamaldehid yang dapat menghambat pertumbuhan bakteri di rongga mulut. Efek ekstrak kulit kayu manis secara in vitro terhadap bakteri di rongga mulut telah sering dilakukan namun pengaruhnya sebagai obat kumur dalam menurunkan indeks plak gigi perlu dikaji. Penelitian ini bertujuan untuk mengetahui efektivitas berkumur dengan larutan ekstrak etanol kulit kayu manis dalam menurunkan akumulasi plak gigi. Kulit kayu manis diekstrak secara maserasi dengan etanol 80%. Obat kumur kulit kayu manis dibuat pada konsentrasi 6.25%. Sampel dalam penelitian ini merupakan responden yang berkunjung di klinik Drg. Syahdiana Waty, Medan, Sumatera Utara. Pengukuran indeks plak gigi dilakukan dua kali yaitu sebelum berkumur dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis. Analisis data yang digunakan adalah uji Wilcoxon untuk mengetahui perbedaan yang signifikan antara indeks plak gigi sebelum berkumur dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis. Hasilnya menunjukkan bahwa ekstrak etanol kulit kayu manis mengandung senyawa metabolit sekunder golongan alkaloida, flavonoid, saponin dan glikosida. Perbedaan indeks plak gigi yang signifikan antara sebelum dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis 0.000 (<0.05). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa larutan ekstrak etanol kulit kayu manis efektif menurunkan akumulasi plak gigi.

Eksplorasi dan Bioasai Berbagai Isolat Bacillus thuringiensis Lokal terhadap Larva Beberapa Jenis Serangga Dwi Suryanto; Chairani Chairani; Deddy Rusika; Nita A. Lubis; Yurnaliza Yurnaliza
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2538

Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.

Kondisi Optimum untuk Produksi Kitinase dari Streptomyces Rkt5 dan Karakterisasi pH dan Suhu Enzim Yurnaliza Yurnaliza; Sebastian Margino; Langkah Sembiring
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i3.2571

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.

Peningkatan produksi petani Jamur Tiram dengan teknik kultur di Kota Medan Yurnaliza Yurnaliza; Liana Dwi Sri Hastuti; Riyanto Sinaga; Adrian Hartanto; Wita E Tarigan
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.335-343

Oyster mushrooms are one of the commodities that people are interested in to meet the needs of healthy and nutritious foods, where the demand of this fungus increases every year. In the city of Medan, oyster mushroom cultivation has begun to develop in the home industry, small to medium scale industries, but in fact it has not yet fulfilled the market demand. The low production of oyster mushrooms caused by the low knowledge of the farmer in preparation of the seed, either F-0, F-1, F-2 or F-3 and ultimately fail in baglog preparation as the final media stage for oyster mushroom production. In the seedlings and backlogs manufacturer should follow the technical culture standard preparation of seedling. Socialization has been carried out by Tim ABDIMAS with the demonstration and introducing culture and sterilization technique using saving energy autoclave, efficient in use for the mushroom farmers and expected after the program the farmers can be confident and become independent in spawn or seedling producing by themselves. The use of autoclave is very effective and significant in reducing the number of contaminated seedlings, as seen in the graph which is also presented in this paper. Knowledge of making mushroom flour with a simple method with low cost in contemporary and hygienic packaging ready to be marketed as a basic ingredient in food processed products or as an ingredient in traditional medicine is also provided.

Bioprospecting of Chitinolytic Diazotroph Rhizospheric Bacterial Isolated From Mucuna bracteata as Biocontrol Against Ganoderma boninense Yurnaliza
International Journal of Ecophysiology Vol. 3 No. 2 (2021): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v3i2.10100

Ganoderma boninense is an infectious phytopathogenic fungus of basal stem rot in oil palm with the need of significant biocontrol strategy or alternative. Bioprospecting of chitinolytic diazotroph bacteria from rhizosphere of Mucuna bracteata as Legume Crop Cover (LCC) in oil palm plantations is potential to investigated. The aims of this study were to obtain total population of diazotroph bacteria and chitinolytic diazotroph bacteria, to evaluate its antagonistic properties followed by identification of the isolate based on 16S rRNA gene encoding. Diazotroph bacteria were isolated from M. bracteata rhizosphere originating from three oil palm plantations (PTPN III, PTPN IV unit Adolina Medan, and Perkebunan rakyat Simalingkar), followed with chitinolytic assay, morphological characterization, antagonistic assay against G. boninense, lytic assay of G. boninense mycelium by using crude chitinase and molecular identification of potential isolate. Total population of diazotroph bacteria in M. bracteata rhizosphere ranged between 2.80–3.83 × 106 CFU/g. Screening of chitinolytic diazotrophs using colloidal chitin medium obtained 23 bacterial isolates and 14 of them were known as antagonists to G. boninense. Five isolates with the highest anti Ganoderma were DK17 (71,15%), DK10 (69,70%), DK07 (59,63%), DK21 (53,48%), and DK19 (52,0%). The highe1st lytic activity of crude chitinase to G. boninense mycelium was produced by DK17. Molecular identification of five potential isolates revealed different identities, e.g. Enterobacter aerogenes (DK07), Mycobacterium senegalense (DK10), Bulkhoderia cepacia (DK17), Pseudomonas stutzeri (DK19), and Bulkhoderia cepacia (DK21). Based on these results, chitinolytic diazotroph bacteria isolated from M. bracteata rhizosphere were able to control G. boninense

Effect of Incubation Time on Amylase Enzyme Activity in Cassava Tape Yurnaliza
International Journal of Ecophysiology Vol. 4 No. 2 (2022): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v4i2.11377

Cassava tape is a product made by a fermentation process that requires the help of microorganisms. The tape fermentation process involves the enzyme amylase. Amylase is a glycoside hydrolase enzyme that catalyzes the breakdown of starch into simple sugars. Enzyme activity can be calculated by measuring the amount of product formed, or by calculating the amount of substrate reduction in a given unit of time. This study aims to determine the effect of incubation time on amylase enzyme activity in cassava tape. The research method used was the descriptive experimental method. The results of the experiment showed a reduction in the substrate due to amylase enzyme activity. The highest percentage of substrate reduction occurred at 48 hours incubation time and the lowest percentage at 144 hours incubation time. An organoleptic test was conducted with color, aroma, texture, and taste parameters conducted on 30 panelists. Incubation time affected the percentage of substrate reduction and organoleptic parameters

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