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All Journal Indonesian Journal of Tropical and Infectious Disease Jurnal Medik Veteriner
Safitri, Erma -
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health Veterinary

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EFFECTIVENESS OF MENIRAN (PHYLLANTHUS NIRURI LINN) AS ANTIBACTERIAL FOR RESISTANCE ANTIBIOTICS PREVENTION OF ENTEROTOXIN ESCHERICHIA COLI Hidanah, Sri; Sabdoningrum, Emy Koestanti; Wahyuni, Retno Sri; Dewi, Arini Rahmi; Safitri, Erma -
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 2 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.153 KB) | DOI: 10.20473/ijtid.v7i2.7328

Abstract

Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which showed 21% resistance to various antibiotics, such as enterotoxigenic E.coli. Alternative efforts are needed to overcome this, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn). Meniran plant is an immunomodulator that serves to repair the immune system of the body. The aim of research is the research was done through several stages: isolation and identification of enterotoxigenic E. coli from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against enterotoxigenic E. coli n The study was divided into five treatments: T0+ (group of chickens were infected by enterotoxigenic E. coli T0- (control group, not infected), T1 (infected by enteroxigenic E. coli + 20% meniran extract), T2 (infected by enterotoxigenic E. coli + 25% extract meniran), T3 (infected by enterotoxigenic E. coli + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results show that all of E. coli DNA isolates which tested by the PCR method show positive reactions at 600 bp. The next stage, the enterotoxigenic E. coli are resistant to some antibiotics, such as amoxicillin, ampicillin, erythromycin, cephalosporins, tetracycline, cloxacillin and gentamicin. Furthermore, the 30% Phyllanthus niruri Linn extract is effective as an antibacterial for antibiotic resistance enterotoxigenic E. coli. next necessary to write that: The 30% meniran extract is potential for kill of enterotoxigenic E. coli
Honey Can Increase The Length of The Small Intestinal Villi in Malnourished Albino Rats Listyorini, Linda; Mustofa, Imam; Hernawati, Tatik; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Safitri, Erma -
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.175-179

Abstract

The aim of this study was to determine the potential of honey to the length of small intestine villi of albino rats due to nutritional deficiencies. The experimental animals were used 24 male albino rats with a body weight of 250-300 grams, divided into four groups and each group contained six replication. Negative control (K-) was a group of rat without fasting and without honey. Positive control (K +) was a group of rat with fasting conditions for 5 days and without honey. The treatment group was the rat with fasting conditions for 5 days and given honey therapy at a dose of 30% (P1) and 50% (P2). Data analysis used ANOVA (Analysis of Variants) then continued with the Duncan Multiple Range test with a significance level of 5% it can be seen that the length of small intestine villi was obtained at (K-) which is 531,321 ± 0,64 which is not significantly different (p>0,05) from (P2), which is 519,919 ± 0,49, but is significantly different (p<0,05) with other treatments (K+) 360,564 ± 0,87 and (P1) 434,275 ± 0,40. The conclusion of this study is that giving honey has the potential to increase the length of the intestinal villi of albino rats due nutritional deficiencies.
Co-Authors Dewi, Arini Rahmi Hidanah, Sri IMAM MUSTOFA Listyorini, Linda Rimayanti Rimayanti Sabdoningrum, Emy Koestanti Tatik Hernawati Tri Wahyu Suprayogi Wahyuni, Retno Sri