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Aluminosilikat Berpotensi Menekan Gangguan Reproduksi Mikotoksin Zearalenon Berdasarkan Pengamatan Jumlah Folikel dan Ekspresi Caspase-9 Ovarium Muhammad Thohawi Elziyad Purnama; Imam Mustofa; Tri Wahyu Suprayogi; Abdul Samik; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.997 KB) | DOI: 10.19087/jveteriner.2017.18.2.175

Abstract

Zearalenone is a resorcylic acid lactone produced by fungal Fusarium graminearum in contaminated edible grains and can cause reproduction disorder in animals by binding to estrogen receptors on target cells. The aim of this study was to assess the potential use of aluminosilicates as mycotoxin binders to eliminate the adverse effect of zearalenone by examining the number of follicles and caspase-9 expression in the ovary of mice. The study adopted a completely randomized simple design using 20 mices which were randomly divided into five group each of which consisted of four mices. Five treatment groups consisted of K+ (without zearalenone and aluminosilicates); K- (treated with zearalenone 0.1 mg/mice/day); P1 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 0.5 mg/mice/day); P2 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 1 mg/mice/day); and P3 were treated with zearalenone 0,1 mg/mice/day and aluminosilicates 2 mg/mice/day) with gastric tube daily for 10 days. The data obtained from this study were analyzed by analysis of variance and proceeded with Duncan test. The result showed that the primary follicles, secondary follicles, tertiary follicles and de Graaf follicles increased significantly on P3 treatment group. Caspase-9 expressions decreased significantly in all of aluminosilicates groups as compared to positive control. The treatment of mice with zearalenone and aluminosilicates increases the number of follicles and decreased caspase-9 expression in the ovary of mice. ABSTRAK Zearalenon merupakan senyawa resorcylic acid lactone yang diproduksi oleh jamur Fusarium graminearum dan dapat mengakibatkan gangguan reproduksi pada ternak dengan membentuk ikatan pada reseptor estrogen. Penelitian ini bertujuan untuk menguji potensi aluminosilikat terhadap mencit yang telah dipapar zearalenon pada aspek jumlah folikel dan ekspresi caspase-9 organ ovarium. Penelitian ini menggunakan 20 ekor mencit yang dibagi menjadi lima kelompok perlakuan dengan masing-masing empat ulangan, yakni K+ tanpa dipapar zearalenon dan aluminosilikat; K- dosis zearalenon 0,1 mg/ekor/hari; P1 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 0,5 mg/ekor/hari; P2 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 1 mg/ekor/hari; P3 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 2 mg/ekor/hari dengan sonde lambung selama sepuluh hari. Data hasil skoring dan perhitungan dianalisis dengan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa jumlah folikel primer, sekunder, tersier dan de Graaf pada P3 terjadi peningkatan yang signifikan. Ekspresi caspase-9 ovarium menunjukkan penurunan pada semua perlakuan aluminosilikat. Simpulan penelitian ini adalah mencit yang dipapar zearalenon dan kemudian diberi aluminosilikat mengalami peningkatan jumlah folikel dan penurunan ekspresi caspase-9 pada organ ovarium.
Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing Lilik Maslachah; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5209.205 KB) | DOI: 10.22146/jpkm.28219

Abstract

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product
TRAINING OF MAKING FEED SUPPLEMENT OF UMB HERBAL IN PARENGAN, TUBAN REGENCY Siti Eliana Rochmi; Herinda Pertiwi; Agung Budianto Achmad; Retno Sri Wahjuni; Tri Wahyu Suprayogi; Tri Bhawono Dadi
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 2 No. 1 (2020): JUNE 2020
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (813.371 KB) | DOI: 10.20473/dc.V2.I1.2020.15-19

Abstract

Background: Subdistrict Parengan has high livestock potential especially Suciharjo Village and Margorejo Village. The existing agricultural waste is not treated properly and livestock cannot reach maximum weights. The provision of quality animal feed is one of the efforts to improve the welfare of the community. Quality of animal feed can be increasing cattle productivity so that the selling value will increase. Purpose: This Community Service activity aims to improve the economic strengthening of the community through breeders through the introduction, dissemination, through technology UMB Herbal based on local resources that can increase the weight gain of beef cattle. UMB Herbal is a concentrate feed that uses raw materials based on raw materials (agriculture) with curcuma which is widely available in the regions. Improving skills makes UMB Herbal can be a provision for the community to be able to increase income. Methods: The method used in community service is the counseling for animal feed and health, training to make UMB Herbal and focus group discussions then applicating of UMB Herbal to pilot cattle. Results: The results of this community service include an increase in beef cattle body weight,  decreasing the gastrointestinal worm infestations, and increasing the knowledge and skills of the participants of the community service. Conclusion: Community Service Activities have a positive impact related to the development of beef cattle farms. 
PENGARUH PEMBERIAN EKSTRAK KULIT BAGIAN DALAM SEMANGKA (Citrullus lanatus) TERHADAP KEUTUHAN MEMBRAN PLASMA DAN ABNORMALITAS MORFOLOGI TIKUS JANTAN (Rattus norvegicus) SETELAH DIPAPAR SUHU PANAS Dinda Reisinta; Widya Paramita L; Tatik Hernawati; Dewa Ketut Meles; Sri Pantja Madyawati; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.658 KB) | DOI: 10.20473/ovz.v7i2.2018.137-142

Abstract

This study aims to determined the effect of watermelon’s inner rind extract (Citrullus lanatus) towards the integrity of the plasma membrane and morphological abnormalities of male Norway rats’s spermatozoa (Rattus norvegicus) after being exposed to hot temperature. The content of lycopene in the inner rind of watermelon serves an antioxidant counteracting free radicals. A total of 20 male Norway rats (Rattus norvegicus) were used in this study with an average age of 2 months. The study was divided into five treatments and four replications, consisting of: K(-) as negative control given CMC Na 0.5% as much as 0.5 ml, P0 as positive control was exposed to 40° C for 1 hour and CMC Na 0.5% of 0.5 ml. P1, P2 and P3 are exposed to 40° C for 1 hour and inner rind extracts of  watermelon with a multilevel dose of P1: 20mg/head/day, P2: 40mg/head/day, and P3: 80mg/head/day that each dose was dissolved in CMC Na 0.5% of 0.5 ml. The results showed that giving inner rind extract of  watermelon to the rats increased the integrity of the plasma membrane and decreased morphological abnormalities of male rat’s spermatozoa (Rattus norvegicus). The result of increased the integrity of the plasma membrane and highest decreased morphological abnormality was shown by P3 group given a dose of 80 mg/head/day.
PENGARUH PENEMBAKAN LASERPUNKTUR PADA TITIK REPRODUKSI ITIK CAMPBELL (Anas platyrhynchos domesticus) BETINA TERHADAP PRODUKTIVITAS TELUR Sena Sangga Renata; Tri Wahyu Suprayogi; A.T Soelih Estoepangestie; R.T. Santanu Adikara; Benjamin Chr. Tehupuring; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.475 KB) | DOI: 10.20473/ovz.v8i1.2019.17-21

Abstract

The technology of laserpuncture in livestock is an acupuncture technique using Laser (Light Amplification by Stimulated Emission of Radiation) that is fired at acupuncture points as biological receptors that are related to organs that can provide stimulatory effects. This research was conducted in the laboratory of Airlangga University for 30 days using a sample of 18 cambell ducks divided into 3 treatments and laser shot with 3 days interval. Treatment 0 as control, treatment 1 got laser treatment dose 0,2 joule and treatment 2 got laser treatment dose 0,5 joule. On the final result of the eggs that have been in the production of duck research for 29 days. P2 given laser shootings with a dose of 0.5 joule yielded more eggs with 158 grains compared to P1 given laser shootings with a dose of 0.2 joules yielding 126 grains and P0 with inactivated laser yielding a total of 86 grains. The conclusion of this study is the shooting of laserpuncture dose of 0.2-0.5 joule can increase egg productivity
HUBUNGAN ANTARA BODY CONDITION SCORE (BCS) DENGAN PRODUKSI SUSU SAPI PERAH FRIESIAN HOLSTEIN (FH) Melsa Netika; Roesno Darsono; Budi Utomo; Imam Mustofa; Ismudiono Ismudiono; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.995 KB) | DOI: 10.20473/ovz.v8i2.2019.89-93

Abstract

The aim of this research was to observe the Body Condition Score (BCS) and milk production of Friesian Holstein (FH) dairy cattles. 144 of dairy cattles divided into 3 groups based on BCS 1-9 scale. The results showed that Y = 6.62 + 5.54X - 5.78X2. Coefficient of determination (R2) of 20.6 this mean any increase in milk production by 20.6% is affected by BCS. It can be concluded that there is a correlation between Body Condition Score (BCS) and milk production. The higher BCS (BCS 7-8) the lower milk production, while in ideal BCS production (5-6) the production of milk is optimize.
PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING Mirza Asafaizh Arini; Tri Wahyu Suprayogi; Sunaryo Hadi Warsito; Trilas Sardjito; Suherni Susilowati; Indah Norma Triana
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.668 KB) | DOI: 10.20473/ovz.v8i2.2019.110-115

Abstract

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 μg / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5μg / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 μg / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Itrah Khoirunnisa; Suherni Susilowati; Lilik Maslachah; Tri Wahyu Suprayogi; Rochmah Kurnijasanti; Hermin Ratnani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.347 KB) | DOI: 10.20473/ovz.v8i2.2019.127-131

Abstract

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.
DAMPAK PEMBERIAN ROTI AFKIR TERHADAP EFISIENSI REPRODUKSI SAPI PERAH Friesian Holstein DI WILAYAH KERJA KPSP SETIA KAWAN NONGKOJAJAR, PASURUAN Ricko Nurdianto S.; Sri Hidanah; Sri Pantja Madyawati; Abdul Samik; wurlina wurlina; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.279 KB) | DOI: 10.20473/ovz.v8i2.2019.144-148

Abstract

The purpose of this research is to know the impact of bread waste to efficiency reproduction of Friesian Holstein cow in regional working KPSP Setia Kawan Nongkojajar, Pasuruan by value Service per Conception (S/C), Conception Rate (CR), Days Open (DO). Method in this research is the dependent variable observed is the reproductive efficiency, the dependent variable as a supporter is the cow has given birth, the healthy dairy cow, the feed volume given. The data obtained is processed by using descriptive analysis to get the reproduction efficiency figure. The result of the research indicate that the value of Service per Conception (S/C) = 1.5 ± 0.69 times, Days Open (DO) = 95.6 ± 11.32 days and Conception Rate (CR) = 65% and Fertility Status (FS) = 72,4%. Results of this study can be concluded that bread waste provided by the breeder as an additional feed for dairy cow has no significant effect.
CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI BALI HASIL INSEMINASI BUATAN DI KABUPATEN SUMBAWA BARAT TAHUN 2017 Asih Deskayanti; Trilas Sardjito; Agus Sunarso; Pudji Srianto; Tri Wahyu Suprayogi; Herry Agoes Hermadi
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.879 KB) | DOI: 10.20473/ovz.v8i2.2019.159-163

Abstract

The study was conducted to know Conception Rate and Service per Conception of  Bali cattle acceptors in west Sumbaw district. Data were collected fot the periode of January to September 2017 from inseminator in west Sumbawa district. The data were taken by primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variable include the identitiy of the breeder, the cages, feeding, drinking and knowladge of breeders. The secondary data were obtained by recording of the officer insemination. All data was analyzed to find a percentage of Conception Rate and find number of Service per Conception (S/C) by analyzed descriptive of SPSS. Risults of this research showed that Conception Rate and Service per Conception’s average of Bali Cattle is 60 % and 1,7. The conculusion of this research showed that Conception Rate is lower than normal value and Service per Conception of Bali cattle in West Sumbawa district is better. This research was expected to be a reference for the next artificial insemination programmed by the government.
Co-Authors A.T Soelih Estoepangestie AA Sudharmawan, AA Abdul Samik Abrian Panggalih Indra Pratama Aditya Irawan Aditya Sukma Pawitra Agung Budianto Achmad Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Al Arif, M Anam Aldin Akbar Rahmatullah Alfiutha Propha Bhaskara Aliyah, F. Himmatul Amaliya, Anny Amrullah, Muhammad Fajar Andrika Indra Pratomo Ani Wijayanti Anisa, Nabila Berlianny Nur Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardina Sahra Miranda Arinda octavia Asih Deskayanti Astrid Annisya Adhy Putri Aswin Rafif Khairullah Ayudani, Saskia Nadhira Az'zahroh, Na'ilah Putri Azhari, Akbar Maulana Belgania, Ristaqul Husna Benjamin Chr. Tehupuring Boedi Setiawan Brahmantya, Chandra BUDI UTOMO Budi Utomo Budi Utomo Candika, Yossy Imam Dadik Rahardjo Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Dinda Reisinta Djoko Legowo Djoko Legowo Dwi Novita Sari Eduardus Bimo Aksono Herupradoto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestianah Epy M. Luqman Erma Safitri Erma Safitri Fajar Ramadhan Subiyantoro Febby Fairy Brillianti Febriani, Nur Rahma Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidayatik, Nanik Himatul Oktavia I. Ismudiono IMAM MUSTOFA Imam Mustofa Indah Norma Triana Indah Norma Triana Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Itrah Khoirunnisa Iwan Sahrial Hamid Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Kadek Rachmawati Khuliyah Candraning Diyanah Kukuh Prastyaningtyas Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lilik Maslachah Linda Listyorini Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lusiandari, Ria Masyitoh, Herlina Mas’ud Hariadi Mas’ud Hariadi Meles, Dewa Ketut Melsa Netika Mirni Lamid Mirza Asafaizh Arini Mufasirin Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nidom, Chairul Anwar Norma Amalia Ulfah Nove Hidajati Nusdianto Triakoso Oky Susandani Palupi, Tantri Dyah Whidi Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Pristafania, Triana Pudji Srianto R.T. Santanu Adikara Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Restiadi, Tjuk Imam Retno Sri Wahjuni Ricko Nurdianto S. Rifky Nugroho Rimayanti Rimayanti Rochmah Kurnijasanti Rochmi, Siti Eliana Roesno Darsono Rofiudin, Muhammad Ilham Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Ardan Saputra, Rakan Mahiid Saputro, Amung Logam Seagames Waluyo, Seagames Sena Sangga Renata Septiana Alafia Yusi Ardiyansyah Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharti Aryanata Suherni Susilowati Suherni Susilowati Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Sunaryo Hadi Warsito Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Trilas Sardjito Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Aulia Ristiani Widya Paramita L Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yossy Imam Candika Yustisiane Ruth Rahadi