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All Journal Jurnal Veteriner Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Mitra Mahajana: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Jurnal Agro Veteriner

Tatik Hernawati

Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3693289

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Pengimbuhan Minyak Jagung pada Pakan Meningkatkan Hirarki Folikuler dan Produktivitas Burung Puyuh (CORN OIL SUPLEMENTATION IN FEED INCREASE FOLICULLAR HIERARCHY AND PRODUCTIVITY OF JAPANESE QUAIL) Herinda Pertiwi; Imam Mustofa; Tatik Hernawati
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to measure the effect of corn oil in feed on estrogen hormone level in blood, hierarchy follicular performance and productivity of Japanese quail. Thirty female Japanese quail were housed in caged and fed ad libitum, the birds were randomly divided into three grouped namely: Po, P1 and the (P0) was fed with commercial feed without corn oil, whereas the first test treatment (P1) was fed with commercial feed mixture containing 3% corn oil, and the second test treatment (P2) was feed with commercial feed mixture containing 6% corn oil along the study. Every day the egg production was recorded. After eight weeks, blood samples were collected from jugular vein to isolate the serum. Then all samples were sacrificed to observe the hierarchy follicular performance. Based on this study it was found that 3% and 6 % corn oil supplementation on feed (P1 and P2) did not cause any significant changes in serum estrogen concentration, and productivity, but had significant changes on hierarchy follicular. This result show that hierarchy follicular increasing due to ovarian activity optimizing induced by polyunsaturated fatty acid (PUFA)by consumed corn oil. Thus, the suplementation of corn oil can be an alternative to increase egg productivity of quail farm. ABSTRAK Penelitian ini bertujuan untuk mengukur pengaruh pemberian minyak jagung dalam pakan terhadap kadar estrogen serum darah, hirarki folikular dan produktivitas burung puyuh. Sebanyak 30 ekor burung puyuh betina dibagi menjadi tiga perlakuan. Perlakuan kontrol diberi pakan komersial tanpa suplementasi minyak jagung, sedangkan perlakuan P1 dan P2 diberi pakan komersial dengan suplementasi minyak jagung sebanyak 3% dan 6%. Setiap hari dilakukan pencatatan terhadap produktivitas. Setelah delapan minggu, dilakukan koleksi sampel darah, kemudian semua burung puyuh dikorbankan nyawanya untuk mengamati hirarki folikular yang terbentuk. Pada P1 dan P2 tidak telihat perbedaan yang nyata terhadap peningkatan kadar estrogen serum darah dan produktivitas dibandingkan dengan P0, akan tetapi terlihat perbedaan nyata pada hirarki folikular yang terbentuk. Hasil ini menunjukkan bahwa peningkatan hirarki folikular yang terbentuk terjadi karena peningkatan kinerja ovarium dipicu oleh polyunsaturated fatty acid (PUFA) yang berasal konsumsi minyak jagung. Dengan demikian, suplementasi minyak jagung dapat dijadikan alternatif untuk meningkatkan produksi telur dalam peternakan burung puyuh.

Penambahan Osteopontin dalam Pengencer Semen Beku Sapi Perah Friesian Holstein Meningkatkan Ekspresi B-Cell Cll/Lymphoma-2 Spermatozoa Postthawing (ADDITIONAL OSTEOPONTIN INTO FROZEN FRIESIAN-HOLSTEIN SEMEN DILUTER INCREASES THE EXPRESSION OF B-CELL CLL/L Abdul Samik; Yudit Oktanella; Tatik Hernawati; Ngakan Made Rai Widjaja; Intan Purwa Dewanti
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.606 KB)

Post thawed frozen semen viability is one of the most important keys in artificial inseminationdepended on two major cell death mechanism, apoptosis and necrosis. It has been known that good fertilitydiary bull’s seminal plasma contains high concentration of osteopontin (OPN). Osteopontin also known ascell survival protein via inhibition to apoptotic cell death, and B-cell CLL/Lymphoma-2 (Bcl-2)expressionmostly related to the ability of cell survival. The aim of this study was to investigate the influence ofadditional OPN into frozen semen diluter on post thawed sperm Bcl-2 expression. Fresh semen collectedfrom ±4 year Friesian Holstein bull. Treatment group divided into four groups i.e.: control group (P0:without OPN supplementation), (P1: fresh semen with OPN supplementation 5 ?g/5.107 spermatozoa), (P2:fresh semen with OPN supplementation 10 ?g/5.107 spermatozoa), (P3: fresh semen with OPNsupplementation 20 ?g/5.107spermatozoa). Bcl-2 sperm expression was determined usingimunocytochemistry. The result of this study showed that there was no significant difference betweengroup P0 and P1 (p>0,05), but both group P2 and group P3 showed a significant difference with P0 (p<0,05).Nevertheless, there was no significant difference between group P2to group P3 on post thawed FriesianHolstein sperm Bcl-2 expression (p>0,05). The conclusion of this study is osteopontin supplement in frozensemen diluter is capable to increase post thawed Friesian Holstein sperm Bcl-2 expression.

Identification of Acanthocephala on Water Monitor Lizard (Varanus salvator) Human Consumed in Sidoarjo Ahmad Herdianto Wicaksono; Emmanuel Djoko Poetranto; Tatik Hernawati; Boedi Setiawan
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Water monitor lizards are consumed by humans for its meat. The demand this reptile consumption because the taste and belief of the people in healing disease. Based on habitat and food, they have the potential to transmit zoonosis and have a high possibility of being infested by parasites. One of the possible disease that could infect water monitor and transmitted to human is acanthocephaliasis, infection from Acanthocephalan. Which previous research found Acanthocephala on intestine of frog. This research aims to know and identify Acanthochepala worms that infect water monitor lizards. Based on the results, 40 samples were used and there are 2 positive samples with Acanthocephalan in the body cavity.

PENAMBAHAN PROTEIN INSULIN LIKE GROWTH FACTOR-I COMPLEX DALAM PENGENCER PEMBEKUAN SEMEN TERHADAP KUALITAS SPERMATOZOA KAMBING PADA WAKTU EKUILIBRASI Suherni Susilowati; Tatik Hernawati
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untuk meningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen. Pada tahap pertama dilakukan identifikasi IGF-I complex dengan menggunakan gel native polyacrylamide gel electrophoresis dan isolasi IGF-I complex. Pada tahap kedua dilakukan aplikasi penambahan protein IGF-I complex pada prosesing semen beku. Semen dikoleksi dengan menggunakan vagina buatan dan kemudian disentrifus selama 5 menit dengan kecepatan 1800 rpm. Kemudian semen dibagi menjadi tiga kelompok. Pada kelompok I, II, dan III ditambahkan protein IGF-I complex masing-masing 0, 12, dan 618 ng/ 3X10 sperma. Selanjutnya dilakukan ekuilibrasi selama 1 jam dan dilanjutkan dengan evaluasi motilitas, viabilitas, dan membran sperma. Hasil penelitian menunjukkan perbedaaan motilitas, viabilitas, dan membran sperma yang signifikan (P0,05) di antara tiga kelompok perlakuan. Dapat disimpulkan bahwa penambahan protein IGF-I complex dapat meningkatkan kualitas semen beku kambing pada fase ekuilibrasi.

Tyrosine Kinase Gene Polymorphisms in Limousin (Bos taurus) Bull Correlation with Fresh Semen Qualities Ageng Ilham Ramadhani; Yudit Oktanella; Wawid Purwatiningsih; Andreas Bandang Hardian; Tatik Hernawati
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.480

Tyrosine kinase (TEK) is the subgroup of protein kinases class. Tyrosine kinase (TEK) is a protein in spermatozoa plasm that functions as a mediator during spermatozoa penetration to pellucide zone 3 (PZ3) of the ovum. The purpose of this study was to analyse the variation of receptor tyrosine kinase genes related to the fresh semen qualities of limousin bulls, using Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) method with HindIII restriction enzyme in A/AGCTT restriction site. This study used 20 blood samples of limousin bull from BBIB Singosari Malang. DNA amplification carried out by PCR method using forward primer (TEK_F) 5'-TAGATTGTCGCTTGCCTGGG-3’ and reverse primer (TEK_R) 5'-CCTGTGCCGACAGGTTTACT-3’. The data analysis were obtained by counting the total of RFLP DNA fragments as the genetic variation. The correlation between the total of RFLP DNA fragments and the fresh semen qualities were analysed by Spearman correlation test with RStudio software. The polymorphic DNA fragments were on 136 bp, 88 bp, 72 bp, and 12 bp range. Monomorphic DNA fragment was on 39 bp range. Correlation coefficient between the RFLP DNA fragments total with semen pH was -0,158 (negative correlation) with the p-value 0,912; semen volume was -0.105 (negative correlation) with the p-value 0,659, sperm motility was -0,050 (positive correlation) with the p-value 0,831; and sperm concentration was 0,044 (positive correlation) with the p-value 0,852. The RFLP DNA fragment total with some fresh semen evaluation parameters had very low correlation with no significancy because of the p-value >0,05.

Perbedaan Sarang Alami dengan Semi Alami Mempengaruhi Masa Inkubasi dan Keberhasilan Menetas Telur Penyu Lekang (Lepidochelys olivacea) Pantai Boom Banyuwangi Samosir, Sabrina Hatoguan; Hernawati, Tatik; Yudhana, Aditya; Haditanojo, Wiyanto
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Penelitian ini bertujuan mengetahui pengaruh kondisi lingkungan dalam aspek pH, suhu dan kelembaban antara sarang alami dengan sarang semi alami terhadap presentase daya tetas dan masa inkubasi telur Penyu Lekang (Lepidochelys olivacea) di kawasan Pantai Boom Banyuwangi. Sampel dipilih dengan metode purposive sampling berupa 20 telur Penyu Lekang yang berasal dari satu induk yang sama. Telur Penyu Lekang yang di inkubasi pada sarang alami dan sarang semi alami masing-masing berjumlah 10 butir. Rancangan penelitian menggunakan metode deskriptif, dengan melakukan observasi terhadap kondisi lingkungan antara sarang alami dengan semi alami. Uji t independent digunakan untuk membandingkan presentase daya tetas. Analisis dilakukan dengan program SPSS. Hasil penelitian menunjukkan presentase daya tetas pada sarang alami berbeda sangat nyata dengan sarang semi alami, sedangkan masa inkubasi tidak dapat diambil data. Presentase daya tetas pada sarang alami lebih baik dibandingkan dengan sarang semi alami. Sarang semi alami memiliki pH yang lebih asam dibandingkan pH pada sarang alami. Simpulan penelitian ini terdapat perbedaan kondisi lingkungan pada aspek pH, suhu, dan kelembaban antara sarang alami dan sarang semi alami yang mempengaruhi masa inkubasi dan daya tetas telur Penyu Lekang.

Pengaruh Pemberian Ekstrak Kedelai (Glycine max) Terhadap Jumlah Pertumbuhan Folikel Ovarium Mencit (Mus musculus) Pramesti, Nugraheni Anggara; Restiadi, Tjuk Imam; Yudhana, Aditya; Hernawati, Tatik; Hamid, Iwan Sahrial
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kedelai (Glycine max) terhadap jumlah pertumbuhan folikel ovarium pada mencit (Mus musculus). Sampel terdiri dari 20 ekor mencit yang secara acak dibagi menjadi lima kelompok. Kelompok kontrol (K) tidak diberi ekstrak kedelai. Kelompok P1 diberi ekstrak kedelai dengan dosis 0,05 mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 0,010 mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 0,015 mg/kgBB. Kelompok P4 diberikan ekstrak kedelai dengan dosis 0,020 mg/kgBB. Ekstrak kedelai diberikan selama 14 hari. Analisis data menggunakan uji One Way ANOVA dan dilanjutkan dengan uji Honestly Significant Difference (HSD). Hasil penelitian menunjukkan kelompok kontrol (K) memiliki perbedaan signifikan dengan kelompok P1, P2, P3 dan P4. Hasil rata-rata jumlah folikel primer tertinggi terdapat pada perlakuan 4 (P4) dengan dosis 0,020 mg/kgBB dan jumlah rata-rata folikel sekunder tertinggi pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB. Penelitian ini dapat disimpulkan bahwa ekstrak kedelai yang diberikan pada mencit betina dapat meningkatkan jumlah pertumbuhan folikel ovarium dan jumlah rata-rata folikel tertinggi adalah pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB.

Honey Can Increase The Length of The Small Intestinal Villi in Malnourished Albino Rats Listyorini, Linda; Mustofa, Imam; Hernawati, Tatik; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Safitri, Erma -
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.175-179

The aim of this study was to determine the potential of honey to the length of small intestine villi of albino rats due to nutritional deficiencies. The experimental animals were used 24 male albino rats with a body weight of 250-300 grams, divided into four groups and each group contained six replication. Negative control (K-) was a group of rat without fasting and without honey. Positive control (K +) was a group of rat with fasting conditions for 5 days and without honey. The treatment group was the rat with fasting conditions for 5 days and given honey therapy at a dose of 30% (P1) and 50% (P2). Data analysis used ANOVA (Analysis of Variants) then continued with the Duncan Multiple Range test with a significance level of 5% it can be seen that the length of small intestine villi was obtained at (K-) which is 531,321 ± 0,64 which is not significantly different (p>0,05) from (P2), which is 519,919 ± 0,49, but is significantly different (p<0,05) with other treatments (K+) 360,564 ± 0,87 and (P1) 434,275 ± 0,40. The conclusion of this study is that giving honey has the potential to increase the length of the intestinal villi of albino rats due nutritional deficiencies.

The Potential of Kaliandra Honey (Calliandra sp.) on Sertoli Cell Counts in Malnourished Albino Rats Erlangga, Made Bagus; Srianto, Pudji; Safitri, Erma; Rimayanti, Rimayanti; Hernawati, Tatik; Hermadi, Herry Agoes
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.88-92

This study was performed to investigate the effect of calliandra honey (Calliandra sp.) on the number of sertoli cells in malnourished male albino rats. A total of 24 male rats were divided into four groups, i.e. (K) without treatment, (P0) fasted, (P1 and P2) respectively fasted and administered honey concentrations of 30% and 50% for 10 days after fasting. The results showed that administration calliandra honey with a concentration of 30% and 50% significantly increased the number of sertoli cells. It can be concluded that administration calliandra honey can have a positive effect on malnourished male rats, especially in improving the number of sertoli cells.

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years Purnawan, Ahmad Budi; Rimayanti, Rimayanti; Susilowati, Suherni; Mustofa, Imam; Hernawati, Tatik; Safitri, Erma
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.162-172

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.

Co-Authors Abdul Samik Abdul Samik, Abdul Ageng Ilham Ramadhani Ahmad Hanif Azzam Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Alifia, Annisa Amaliya, Anny Anas Bad’rosana Prawindi Ani Wijayanti Annisa Nur Fitria Rahma Anwar Ma'ruf Boedi Setiawan BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Dadik Rahardjo Dadik Rahardjo, Dadik Dewi, Aprilia Kurnia Djoko Legowo Eka Pramyrtha Hestianah Emmanuel Djoko Poetranto Emy Koestanti Sabdoningrum Erlangga, Made Bagus Erma Safitri Estoepangestie, A.T.Soelih Ferdiansyah, Muhammad Billy Haditanojo, Wiyanto Hani Plumeriastuti Hardian, Andreas Bandang Herinda Pertiwi Hermadi, Herry Agus Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Imam Mustofa Indah Norma Triana, Indah Norma Intan Purwa Dewanti Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Koesnoto Supranianondo Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani Mas'ud Hariadi Meles, Dewa Ketut Meydilasari, Nanda Irsa Moh. Zaky ‘Ubaidillah Muhammad Ikmal Robih Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nanik Hidayatik Ngakan Made Rai Widjaja Niken Meyliana Sari Nove Hidajati Nurhusien Yimer Paramita L, Widya Pitaloka, Dinda Galuh Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Prastyorini, Vina Ari Pudji Srianto Purnawan, Ahmad Budi Reisinta, Dinda Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Rochmah Kurnijasanti Rosyada, Zulfi Nur Amrina Safitri, Erma - Samosir, Sabrina Hatoguan Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Sherina Lashita Candrakirana Soeharsono Soeharsono Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistyowati, Deny Supranianondo, Koesnoto Supriyadi Supriyadi Supriyadi Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tsaniya Atikahani Shabirah Utama, Dirga Januar Surya Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Wawid Purwatiningsih Widiantoro, Krisna Widiyatno, Thomas V. Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yudit Oktanella, Yudit

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