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All Journal Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Mitra Mahajana: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Jurnal Agro Veteriner

Tatik Hernawati

Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3693289

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Identification of Acanthocephala on Water Monitor Lizard (Varanus salvator) Human Consumed in Sidoarjo Ahmad Herdianto Wicaksono; Emmanuel Djoko Poetranto; Tatik Hernawati; Boedi Setiawan
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Water monitor lizards are consumed by humans for its meat. The demand this reptile consumption because the taste and belief of the people in healing disease. Based on habitat and food, they have the potential to transmit zoonosis and have a high possibility of being infested by parasites. One of the possible disease that could infect water monitor and transmitted to human is acanthocephaliasis, infection from Acanthocephalan. Which previous research found Acanthocephala on intestine of frog. This research aims to know and identify Acanthochepala worms that infect water monitor lizards. Based on the results, 40 samples were used and there are 2 positive samples with Acanthocephalan in the body cavity.

PENAMBAHAN PROTEIN INSULIN LIKE GROWTH FACTOR-I COMPLEX DALAM PENGENCER PEMBEKUAN SEMEN TERHADAP KUALITAS SPERMATOZOA KAMBING PADA WAKTU EKUILIBRASI Suherni Susilowati; Tatik Hernawati
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untuk meningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen. Pada tahap pertama dilakukan identifikasi IGF-I complex dengan menggunakan gel native polyacrylamide gel electrophoresis dan isolasi IGF-I complex. Pada tahap kedua dilakukan aplikasi penambahan protein IGF-I complex pada prosesing semen beku. Semen dikoleksi dengan menggunakan vagina buatan dan kemudian disentrifus selama 5 menit dengan kecepatan 1800 rpm. Kemudian semen dibagi menjadi tiga kelompok. Pada kelompok I, II, dan III ditambahkan protein IGF-I complex masing-masing 0, 12, dan 618 ng/ 3X10 sperma. Selanjutnya dilakukan ekuilibrasi selama 1 jam dan dilanjutkan dengan evaluasi motilitas, viabilitas, dan membran sperma. Hasil penelitian menunjukkan perbedaaan motilitas, viabilitas, dan membran sperma yang signifikan (P0,05) di antara tiga kelompok perlakuan. Dapat disimpulkan bahwa penambahan protein IGF-I complex dapat meningkatkan kualitas semen beku kambing pada fase ekuilibrasi.

Perbedaan Sarang Alami dengan Semi Alami Mempengaruhi Masa Inkubasi dan Keberhasilan Menetas Telur Penyu Lekang (Lepidochelys olivacea) Pantai Boom Banyuwangi Samosir, Sabrina Hatoguan; Hernawati, Tatik; Yudhana, Aditya; Haditanojo, Wiyanto
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Penelitian ini bertujuan mengetahui pengaruh kondisi lingkungan dalam aspek pH, suhu dan kelembaban antara sarang alami dengan sarang semi alami terhadap presentase daya tetas dan masa inkubasi telur Penyu Lekang (Lepidochelys olivacea) di kawasan Pantai Boom Banyuwangi. Sampel dipilih dengan metode purposive sampling berupa 20 telur Penyu Lekang yang berasal dari satu induk yang sama. Telur Penyu Lekang yang di inkubasi pada sarang alami dan sarang semi alami masing-masing berjumlah 10 butir. Rancangan penelitian menggunakan metode deskriptif, dengan melakukan observasi terhadap kondisi lingkungan antara sarang alami dengan semi alami. Uji t independent digunakan untuk membandingkan presentase daya tetas. Analisis dilakukan dengan program SPSS. Hasil penelitian menunjukkan presentase daya tetas pada sarang alami berbeda sangat nyata dengan sarang semi alami, sedangkan masa inkubasi tidak dapat diambil data. Presentase daya tetas pada sarang alami lebih baik dibandingkan dengan sarang semi alami. Sarang semi alami memiliki pH yang lebih asam dibandingkan pH pada sarang alami. Simpulan penelitian ini terdapat perbedaan kondisi lingkungan pada aspek pH, suhu, dan kelembaban antara sarang alami dan sarang semi alami yang mempengaruhi masa inkubasi dan daya tetas telur Penyu Lekang.

Pengaruh Pemberian Ekstrak Kedelai (Glycine max) Terhadap Jumlah Pertumbuhan Folikel Ovarium Mencit (Mus musculus) Pramesti, Nugraheni Anggara; Restiadi, Tjuk Imam; Yudhana, Aditya; Hernawati, Tatik; Hamid, Iwan Sahrial
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kedelai (Glycine max) terhadap jumlah pertumbuhan folikel ovarium pada mencit (Mus musculus). Sampel terdiri dari 20 ekor mencit yang secara acak dibagi menjadi lima kelompok. Kelompok kontrol (K) tidak diberi ekstrak kedelai. Kelompok P1 diberi ekstrak kedelai dengan dosis 0,05 mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 0,010 mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 0,015 mg/kgBB. Kelompok P4 diberikan ekstrak kedelai dengan dosis 0,020 mg/kgBB. Ekstrak kedelai diberikan selama 14 hari. Analisis data menggunakan uji One Way ANOVA dan dilanjutkan dengan uji Honestly Significant Difference (HSD). Hasil penelitian menunjukkan kelompok kontrol (K) memiliki perbedaan signifikan dengan kelompok P1, P2, P3 dan P4. Hasil rata-rata jumlah folikel primer tertinggi terdapat pada perlakuan 4 (P4) dengan dosis 0,020 mg/kgBB dan jumlah rata-rata folikel sekunder tertinggi pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB. Penelitian ini dapat disimpulkan bahwa ekstrak kedelai yang diberikan pada mencit betina dapat meningkatkan jumlah pertumbuhan folikel ovarium dan jumlah rata-rata folikel tertinggi adalah pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB.

Honey Can Increase The Length of The Small Intestinal Villi in Malnourished Albino Rats Listyorini, Linda; Mustofa, Imam; Hernawati, Tatik; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Safitri, Erma -
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.175-179

The aim of this study was to determine the potential of honey to the length of small intestine villi of albino rats due to nutritional deficiencies. The experimental animals were used 24 male albino rats with a body weight of 250-300 grams, divided into four groups and each group contained six replication. Negative control (K-) was a group of rat without fasting and without honey. Positive control (K +) was a group of rat with fasting conditions for 5 days and without honey. The treatment group was the rat with fasting conditions for 5 days and given honey therapy at a dose of 30% (P1) and 50% (P2). Data analysis used ANOVA (Analysis of Variants) then continued with the Duncan Multiple Range test with a significance level of 5% it can be seen that the length of small intestine villi was obtained at (K-) which is 531,321 ± 0,64 which is not significantly different (p>0,05) from (P2), which is 519,919 ± 0,49, but is significantly different (p<0,05) with other treatments (K+) 360,564 ± 0,87 and (P1) 434,275 ± 0,40. The conclusion of this study is that giving honey has the potential to increase the length of the intestinal villi of albino rats due nutritional deficiencies.

The Potential of Kaliandra Honey (Calliandra sp.) on Sertoli Cell Counts in Malnourished Albino Rats Erlangga, Made Bagus; Srianto, Pudji; Safitri, Erma; Rimayanti, Rimayanti; Hernawati, Tatik; Hermadi, Herry Agoes
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.88-92

This study was performed to investigate the effect of calliandra honey (Calliandra sp.) on the number of sertoli cells in malnourished male albino rats. A total of 24 male rats were divided into four groups, i.e. (K) without treatment, (P0) fasted, (P1 and P2) respectively fasted and administered honey concentrations of 30% and 50% for 10 days after fasting. The results showed that administration calliandra honey with a concentration of 30% and 50% significantly increased the number of sertoli cells. It can be concluded that administration calliandra honey can have a positive effect on malnourished male rats, especially in improving the number of sertoli cells.

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years Purnawan, Ahmad Budi; Rimayanti, Rimayanti; Susilowati, Suherni; Mustofa, Imam; Hernawati, Tatik; Safitri, Erma
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.162-172

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.

Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing Wijayanti, Ani; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Hernawati, Tatik; Sardjito, Trilas; Saputro, Amung Logam; Amaliya, Anny; Sulistyowati, Deny
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.66-74

This study aimed to determine the effect of adding 96% ethanol extract of green tea (Camellia sinensis) in egg yolk tris diluter on the quality of spermatozoa of Bali cattle (Bos sondaicus) after freezing. The samples were divided into four different treatment groups, which contained semen sample + 13% glycerol + each egg yolk tris diluter + green tea extract (P0) 0 mg/100 ml, (P1) 0,05 mg/100 ml, ( P2) 0,10 mg/100 ml, and (P3) 0,15 mg/100 ml. The variables observed included motility, viability, and spermatozoa abnormalities. Data were analyzed using OneWay ANOVA and continued with Duncan's test (p<0,05). The results showed that the P3 group had motility of 49,00 ± 2,15, viability of 61,92 ± 1,70, and abnormality of 4,24 ± 2,16 significantly compared to the other groups. In conclusion, the P3 group with the addition of 0,15 mg/100 ml of green tea could increase the motility, viability, and abnormal spermatozoa of Bali cattle.

The addition of duck egg yolk in skim milk – fructose extender maintains sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage Isti Annisa Ramahtia; Tatik Hernawati; Tri Wahyu Suprayogi; Tjuk Imam Restiadi; Djoko Legowo; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.1-8

This study aimed to determine the addition of duck egg yolk in skim milk – fructose extender on sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage. Ejaculates of Muscovy duck were divided into four portions equally. The first portion was for the control group (diluted in skim milk – fructose extender), and the second, third, and fourth portions were for the treatment groups of T1, T2, and T3 (diluted in skim milk – fructose extender added with 2.5, 5.0, and 7.5% v/v duck egg yolk). Sperm motility and viability were evaluated two hourly until the motility percentage reached 40%. The data were analyzed using ANOVA and Duncan test. The result showed that the highest percentage of spermatozoa motility and viability (p<0.05) was in the T2 group compared to the other groups. It could be concluded that the Muscovy duck semen extended in skim milk - fructose with 2.5 to 7.5% egg yolk of duck qualified for artificial insemination at storage up to 10 hours in 5°C storage.

The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.

Co-Authors Abdul Samik Abdul Samik, Abdul Ageng Ilham Ramadhani Ahmad Hanif Azzam Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Alifia, Annisa Amaliya, Anny Anas Bad’rosana Prawindi Ani Wijayanti Annisa Nur Fitria Rahma Anwar Ma'ruf Boedi Setiawan BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Dadik Rahardjo Dadik Rahardjo, Dadik Dewi, Aprilia Kurnia Djoko Legowo Eka Pramyrtha Hestianah Emmanuel Djoko Poetranto Emy Koestanti Sabdoningrum Erlangga, Made Bagus Erma Safitri Estoepangestie, A.T.Soelih Ferdiansyah, Muhammad Billy Haditanojo, Wiyanto Hani Plumeriastuti Hardian, Andreas Bandang Herinda Pertiwi Hermadi, Herry Agus Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Imam Mustofa Indah Norma Triana, Indah Norma Intan Purwa Dewanti Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Juliano, Raffael Darrel Kadek Rachmawati Koesnoto Supranianondo Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani Mas'ud Hariadi Meles, Dewa Ketut Meydilasari, Nanda Irsa Moh. Zaky ‘Ubaidillah Muhammad Ikmal Robih Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nabila Ayu Saraswati Nanik Hidayatik Ngakan Made Rai Widjaja Niken Meyliana Sari Nove Hidajati Nurhusien Yimer Paramita L, Widya Pitaloka, Dinda Galuh Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Prastyorini, Vina Ari Pudji Srianto Purnawan, Ahmad Budi Reisinta, Dinda Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Rochmah Kurnijasanti Rosyada, Zulfi Nur Amrina Safitri, Erma - Samosir, Sabrina Hatoguan Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Sherina Lashita Candrakirana Soeharsono Soeharsono Sosilowati, Suherni Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistyowati, Deny Supranianondo, Koesnoto Supriyadi Supriyadi Supriyadi Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tsaniya Atikahani Shabirah Utama, Dirga Januar Surya Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Wawid Purwatiningsih Widiantoro, Krisna Widiyatno, Thomas V. Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yudit Oktanella, Yudit

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